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Ionic Liquid 1-Vinyl-3-dodecylimidazole Bromide Embedded Hybrid Monolithic Column and Its Versatile Post-modification with Amino Acids

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Abstract

The amino acids post-modified ionic liquids-based hybrid monolithic columns were successfully developed. For the first step, using 1-vinyl-3-dodecylimidazole bromide (VDI·Br) and polyhedral oligomeric silsesquioxane methacryl substituted (POSS-MA) as monomer and crosslinking agent, the monolithic POSS-VDI matrix material was polymerized in 100 μm capillary. The prepared POSS-VDI column is of ion-exchange/reverse-phase mixed-mode retention characteristics. The column efficiency reached 137,000 N/m for thioureas. The POSS-VDI column has also been successfully applied to recognize and analyze the effective components of Traditional Chinese Medicines Fangji and the Root of Kudzu Vine. Furthermore, based on its good chromatographic performances, in the second step, amino acids l-Proline (l-Pro), Glycine (Gly) or DL-Phenylalanine (DL-Phe) were further exploited as the anion exchangers to post-modify the POSS-VDI column. The new POSS-VDI-Pro, POSS-VDI-Gly and POSS-VDI-Phe columns exhibited different separation selectivity for the complex egg white protein sample. The present work provides a good route to fabricate versatile amino acids and ionic liquids modified monolithic columns with good separation selectivity.

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Funding

We thank financial support from Hundred Outstanding Innovative Talents in Universities of Hebei Province (SLRC2019016), Natural Science Foundation of Hebei Province (B2019201327), Young Talent of Hebei Province, National Natural Science Foundation of China (21675039) and Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (2015116).

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Authors and Affiliations

  1. College of Pharmaceutical Sciences, Key Laboratory of Public Health Safety of Hebei Province, Hebei University, Baoding, 071002, China

    Yufeng Zhou, Yixin Huang, Yaqi Liu, Lijuan Wang & Xiaoqiang Qiao

  2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China

    Yufeng Zhou, Lijuan Wang & Xiaoqiang Qiao

  3. The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China

    Liguang Duan

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  1. Yufeng Zhou
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  2. Liguang Duan
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Correspondence to Xiaoqiang Qiao.

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Zhou, Y., Duan, L., Huang, Y. et al. Ionic Liquid 1-Vinyl-3-dodecylimidazole Bromide Embedded Hybrid Monolithic Column and Its Versatile Post-modification with Amino Acids. J. Anal. Test. 5, 387–395 (2021). https://doi.org/10.1007/s41664-021-00171-2

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