Uncertainty Analysis in Life Cycle Assessments Applied to Biorefineries Systems: A Critical Review of the Literature

The aim of the present article was to investigate the relationship between uncertainty analysis and life cycle assessment (LCA) in scientific publications that address the application of LCA in biorefineries systems. Uncertainty analysis and its relationship with environmental impact assessment studies, especially those that address the application of LCA, is a research topic that requires attention because of its possible influence on results. A reference base was defined using a systematic approach and bibliometric analysis, with 64 scientific publications extracted from the Web of Science (WoS) and Scopus® databases, which were analyzed using two computational tools: VOSviewer and SciMAT. This group of publications helped establish the correlation and evolution over the last 10 years of the three key themes: “uncertainty analysis,” “LCA,” and “biorefineries.” The results of bibliometric analysis for the established framework pointed to a close and important relationship among these themes. The results were presented quantitatively and qualitatively, and the latter were visualized using infographics, co-occurrence networks, and strategic keyword diagrams. Although the study confirmed the relevance of uncertainties analysis to support LCA studies, it was identified a secondary role for scientific studies analyzed. The study also presents the analysis and discussions of the main publications found in the scientific literature. Future studies should conduct a more in-depth analysis of advanced knowledge representation and reasoning strategies about uncertainty, such as probabilistic ontologies.


Introduction
The environmental performance of products and services has become an important factor in the new economic context that has been emergent in recent years. This context is guided by the principles of bioeconomies and their relationships with circular economies. A bioeconomy is defined as an economy whose basic pillars of production, such as materials, chemicals, and energy products, are derived from renewable sources, providing alternatives to fossil fuel-based products and energy (European Commision 2015; Embrapa 2018). Circular economy is defined by Saavedra et al. (2018) as the study of real-life nonlinear systems to facilitate efficient flows of materials, energy, work, and information, promoting circular flows that reduce environmental impacts and optimize resource efficiency. In circular economies, the economic and environmental value of materials is preserved as long as possible, keeping them in the economic system, whether by increasing the lifetime of the products derived from them or returning them to the system for reuse (den Hollander et al. 2017;Saavedra et al. 2018). The relevance of these two concepts is that both offer approaches and challenges for handling environmental impacts, such as climate change, land and water use, and toxic emissions, which are the target of discussions in forums around the world, such as the United Nations Climate Change Conference and Europe's Bioeconomy Strategy.
Several tools can be used to measure these impacts: life cycle assessment (LCA), strategic environmental assessment (SEA), environmental impact assessment (EIA), environmental risk assessment (ERA), cost-benefit analysis (CBA), material flow analysis (MFA), and the ecological footprint (EF) method. The studies of Finnveden et al. (2009) and Stavropoulos et al. (2016) showed that LCA is one of the most well-known approaches. According to Finnveden et al. (2009) and Klöpffer (2014), interest in LCA grew quickly in the 1990s, and during this period, two of the method's main features were presented: (1) comparison of production systems using a functional unit, e.g., a 1000 L container of a beverage and its transportation to the sales point, and (2) the possibility of cradle-to-grave analysis, including assessment of all the important phases of a product or service's life cycle. Life cycle assessment has been applied in several cases. Cherubini and Ulgiati (2010), presented a review of the assessment of potential environmental impacts of biomass-derived energy. You et al. (2012) determined greenhouse gas (Gg) balance, global carbon balance, and renewable energy returns in comparison with fossil fuel inputs. There have also been studies assessing the environmental impact of the production of bioproducts and renewable chemicals that can be obtained from biorefinery systems (Yue et al. 2014;Carneiro et al. 2017), among others.
The LCA technique identifies potential environmental impacts, and in this context, it does not predict absolute or precise impacts because, among other factors, of uncertainties inherent in data collection and modeling of these impacts (Klöpffer 2014). An adequate analysis of these uncertainties helps prioritize the choices made throughout the study of impact analysis using LCA. Identifying these uncertainties, as well as their sources and types, allows for increased data transparency and reliability of results, preventing unfeasible or misguided decisions (Baker and Lepech 2009;Curran 2012).
Sources of uncertainty have been defined in Finnveden et al. (2009) as input data for LCA methods that may be uncertain and directly related to several types of uncertainty. Uncertainty in LCA studies can stem from various sources, including low quality and/or absence of data, system limitations, and allocation principles; the time frame adopted for impact assessment; assumptions based on the wrong models; and phenomena in domains subject to statistical fluctuations (Curran 2012;Guo and Murphy 2012;Gargalo et al. 2016;Carvalho et al. 2016).
Types of uncertainty can be understood as aspects that can be wrong (unreliable) if derived from these sources (Finnveden et al. 2009). Some examples of types of uncertainty include choices inconsistent with the goal and scope of the impact assessment study; technological evolution; changes in incentives and government policy; different allocation methods for different supply chains; weather variations; supply of raw materials (feedstocks); and price and cost volatility (Frischknecht et al. 2005;Finnveden et al. 2009;Awudu and Zhang 2012;You et al. 2012;Yue et al. 2014;Mirkouei et al. 2017).
The main goal of the present study was to identify the extent to which uncertainty analysis is addressed in impact assessment studies using the LCA method applied to technological systems defined as biorefineries, through a systematic and bibliometric specialized literature review. In addition, the study indicates the most cited authors and publications, providing their main outputs. Table 1 presents the definitions adopted for the three key terms used in this study: uncertainty analysis, LCA, and biorefinery.

Methodology
The methodology used in this survey consisted of three phases. (1) systematic approach, (2) bibliometric analysis, and (3) discussion of the results of phase 2 (Cronin et al. 2008;Saavedra et al. 2018).
Phase 1 was further divided into three steps: (1) defining the conceptual problem, (2) adopting inclusion and exclusion criteria, and (3) data collection. In step 1, the terms life cycle assessment*, life cycle assessment*, life cycle analy*, life cycle analy*, life cycle assessment (LCA), LCA, uncertaint*; uncertaint* analy*; bioref*, and bio-ref* were established as the keywords to qualify the themes uncertainty analysis, LCA, and biorefineries. In steps 2 and 3, only publications written in English from two scientific literature databases were analyzed, the Web of Science (WoS) and Scopus®, starting from the first year available in the database up to the year of the study. Because the WoS does not allow for direct searches in its keyword field, which is different from the Scopus® database, the Boolean operators "or" and "and" were used in the search fields identified as "Topic" (WoS) and "Title-Abstract-Keywords" (Scopus®). These fields are equivalent.
Using these parameters, 55 publications were found in WoS and 33 in Scopus®, being that the WoS included 72.7% of the results found in Scopus®. For this reason, the WoS was chosen as the reference base for the computational data analysis tools. However, to ensure that the results of both databases were considered in the study, the remaining nine publications (27.3%) were also included. Thus, the basis of reference consisted of 64 publications. The search was conducted in the third week of October 2018.
In Phase 2, bibliometric analysis was conducted to characterize, structure, and quantitatively analyze the field of research and the impact of a set of researchers, for a set of scientific journals or a given publication (Cobo et al. 2011;Gutiérrez-Salcedo et al. 2017;Marques et al. 2018). This approach has been used in bibliographic reviews in the very different fields of knowledge. van Leeuwen (2006)  . The present study used VOSviewer (version 1.6.9) and SciMAT (version 1.1.04); both are opensource software and compatible with the WoS and Scopus® database formats. The VOSviewer is a tool for constructing and visualizing bibliometric networks, presenting correlations between the analyzed data (such as authors, keywords and journals), while SciMAT provides important characteristics that allow for data preprocessing, statistical analysis, graphic representation of results, and clustering algorithms for complete bibliometric analysis. A complete review of the features of these tools is presented by Gutiérrez-Salcedo et al. (2017).
The VOSviewer was set up as follows: (1) a thesaurus file with equivalent terms was created; (2) instead of full counting, a fractional counting algorithm was applied, based on the methodology presented by Perianes-Rodriguez et al. (2016); (3) a minimum number of keyword occurrences were set (V min. occurr. ), which indicates the number of publications in which the keyword appeared at least once, and V min. occurr. = 5 was defined to exclude terms with a low number of occurrences (V min. occurr. < 5) and to avoid the indication of a low number of terms, restricting the analysis set (V min. occurr. > 5); and (4) the identified terms were conceptualized as "main keywords." The SciMAT program was set up in three steps: (1) selecting time periods for analysis, (2) eliminating false positives and negatives, and (3) defining calibration parameters for the bibliometric analysis algorithms. In Step 1, the analyzed time frame was split into two periods: 2008-2014 and 2015-2018. This allowed for a good distribution of publications for scientific mapping analysis, with a total of 26 publications in 2008and 38 publications in 2015-2018 Step 2, search algorithms were used for plurals and distance, which were integrated into the tool, thus grouping similar keywords such as biofuel and biofuels, biorefinery and biorefineries, LCA and life-cycle assessment, and uncertainty and uncertainties. In this step, keywords that were not meaningful in the context of the study or were too broad in meaning were excluded, such as model, case study, design, challenges, perspective, methodology, technologies, management, and system. Next, in Step 3, the keyword was selected as the unit of analysis, and the other parameters were defined based on the methodologies presented by Cobo et al. (2011), Cavalcanti (2016), Burnard et al. (2017), and Castillo-Vergara et al. (2018).
Phase 3 consisted of data processing and presenting the results in both quantitative (descriptive) and qualitative (content) formats, following the methodology proposed by Castillo-Vergara et al. (2018) and Cobo et al. (2011Cobo et al. ( , 2012Cobo et al. ( , 2018. Descriptive analysis showed the main areas of related research areas, the main authors, publications, journals, and the geographical scope of the scientific productions. For content analysis, the main correlations and thematic evolutions among the themes uncertainty analysis, LCA, and biorefineries were presented using infographics, keyword co-occurrence networks, strategic diagrams and analysis, and discussion of the most-cited publications for the three main themes identified in each time frame assessed in the present study.  Tang et al. 2015). In addition to this definition, it is also important to consider the sensitivity analysis concept as an approach to identify which input parameters carry out more or less influence on the final results on LCA model (Baker and Lepech 2009) LCA A method that assesses the environmental impact of a given product or service throughout its lifetime; its goal is to make the environmental performance of products and services comparable, in addition to allowing consumers to choose the ones with the lowest burden. Four phases are applied when using this technique, in conformity with ISO 14040 (principles and structure) and 14044: definition of scope and objective, inventory (data collection), impact assessment, and interpretation (Rebitzer et al. 2004;Guinée et al. 2011;Curran 2012;Klöpffer 2014;Stavropoulos et al. 2016) Biorefineries Industrial plants that carry out sustainable conversion processes of biomass into products (such as chemical materials and feedstock) and/or energy (electricity, fuel, and heat). Biorefinery systems can mitigate the impacts of climate change, reduce dependence on fossil fuels, and increase cleaner supply chains based on the sustainable processing of biomass (

Results and Discussion
Descriptive Analysis Figure 1 depicts the 10 main areas of research identified, with engineering representing 37 studies (58%) of the total of 64 publications in the basis of reference. The studies in this area focused on chemical and biochemical engineering, especially because of the relevance of the challenges, issues, and uncertainties related to the biomass supply chain for energy conversion, biofuels, and value-added coproducts. The figure shows that the total number of documents was higher than that of publications; i.e., the same publication could be related to more than one field of research. Table 2 lists the five main authors with the most published studies, classified according to their respective impact factors (h-index or HI). The first two authors were Fengqi You from Cornell University, with studies about biofuel and bioenergy supply chain modeling and optimization, and Gürkan Sin from the Technical University of Denmark, with studies about uncertainty analysis and risk management in environmental sustainability processes. Considering a total of the 17 publications listed in the table, most authors were affiliated with institutions in the USA, accounting for 53% of the scientific production. Table 3 presents the five most-cited publications. The top two were by Cherubini et al. (2009) and addressed issues regarding LCA studies for biofuel and bioenergy and relevant recommendations, while You et al. (2012) investigated models for optimizing biofuel supply chains. Figure 2 presents the top ten journals, their respective numbers of publication, and impact factors. These journals included 51.5% of the total number of publications, and the Journal of Cleaner Production headed the list with six publications in the research area and an impact factor of 5.651. Figure 3 shows the ten countries with the highest number of publications. The USA and Denmark accounted for 44% and 12.5%, respectively, while Brazil represented 6.3% of the total. The countries identified on the map in descending order of publications are USA (28), Denmark (8), Brazil, China and Portugal (4 each), and Austria, Holland, France, Spain, and Belgium (3 each). Figure 4 presents an infographic with the main keywords extracted by VOSviewer. The top five terms were LCA (37), biofuels (31), energy (21), and biomass and biorefinery (18 each), while uncertainty and uncertainty analysis resulted in 13 and 5 occurrences, respectively. It is worth noting that the low occurrence of a keyword does not imply irrelevance or lack of correlation with the other main keywords. Figure 5 illustrates the correlations among the main keywords using a co-occurrence network, in which the thickness of the lines indicates the strength of the correlations between the nodes (keywords), calculated by counting the number of publications in which the two keywords occur together. The size of the nodule reveals its weight (representativeness) in the network, which is determined by the total number of occurrences of the node. The network clearly shows a connection among the three keywords investigated in this study (uncertainty analysis, LCA, and biorefinery) through the strength of correlations among the keywords LCA, biofuels, energy, biomass, and biorefinery and, at a lower level, between the keywords uncertainty analysis and LCA, uncertainty and LCA, and biofuels and biorefinery. Van Eck and Waltman (2018) established the following rules for adequately interpreting a co-occurrence network: (1) the closer to yellow and green the node, the higher its relevance in the network; (2) the numeric scale presented in the lower right corner of the figure presents the average number of citations of publications To visualize the thematic evolution of the main keywords, Fig. 6 presents a strategic diagram for each time period. A strategic diagram is a bidimensional graphic representation that shows the thematic nuclei (most representative keywords) arranged in four groups and measured according to two standards: centrality (level of interaction) and density (weight). The greater the centrality and density, the more relevant the thematic nucleus in the context of the study. The groups are represented by the following quadrants: (1) motor themes (upper right quadrant), which are well-developed themes that are important to structuring a field of research; (2) basic and transversal themes (lower right quadrant), which are important to a field of research but not sufficiently developed; (3) themes that are emergent or decadent (lower left quadrant); (4) peripheral and isolated themes (upper left quadrant); or (5) themes that are internally well-developed but isolated from other themes and are of marginal importance to developing the field of research.

Content Analysis
The study of Cobo et al. (2018) established that (1) the main thematic nuclei are formed by well-defined motor and basic themes and that (2) the diameter of the sphere is proportional to the number of publications that cite each thematic nucleus. Figure 6 shows that in 2008-2014, three main thematic nuclei stood out, and of these, biofuels and biorefinery were clearly identified as motor themes, while biomass emerged as a basic theme. In 2015-2018, three main thematic nuclei also emerged, in which the LCA was a motor theme, and biofuels and biomass remained as motor and basic themes, respectively. In both periods, the term supply chain evolved as a motor theme (high density and moderate centrality), defined mainly by analysis in scenarios uncertainty (Nguyen et al. 2014;Osmani and Zhang 2014;Tong et al. 2014); impact assessments involving environmental, social, and economic aspects (Bakshi 2014); and supply chain optimization studies and also scenarios uncertainty, especially in terms of the pathways for biofuel and energy production inputs (Malça and Freire 2010;Awudu and Zhang 2012;You et al. 2012;Gong and You 2014).
The term biorefinery, which was considered a motor theme in 2008-2014, turned to be a peripheral theme in 2015-2018. It may suggest that the research related to the topic shifted from a concept development scenario to a more consolidated theme, especially because of emerging practical applications and its undisputed relevance in studies related to bioeconomy, especially in Europe, as can be seen in the European plan of action for the circular economy (European Commision 2015). Considering this consolidation of the term biorefinery, the term LCA evolved as a motor theme in 2015-2018. One factor that explains this thematic evolution is the advent of the circular economy for the business world in mid-2014, indicating that sustainability and environmental impact reduction as strategic requirements for the life cycle perspective in each step of the value chain in the process production of products and servicese.g., feedstocks supply chain, transportation logistics, and waste management (Ellen MacArthur Foundation 2014; European Commision 2015; CNI 2018; Saavedra et al. 2018).
The themes uncertainty analysis and uncertainty were not identified as the main thematic nuclei in any of the analyzed periods. Together with the low occurrence of these themes (Figs. 4 and 5), this demonstrates that although it is an important issue, uncertainty analysis in LCA is a secondary study and has not been cited as frequently in the keywords of  (2010) International Journal of Life Cycle Assessment publications, or is not commonly conducted in practice, and when it is, it still represents a lower percentage of studies (Gargalo et al. 2016). The theme technoeconomic analysis saw a decline between the analyzed periods, in addition to low occurrence (Figs. 4 and 5). However, it is necessarily addressed in studies that discuss, among other things, economic performance in the production of products derived from renewable resources (Paap et al. 2013;Pourhashem et al. 2013).
Tables 4 and 5 present the performance of each of the main thematic nuclei (TN), considering the following measurements: number of publications (NP), citations (CT), h-index (HI), centrality, and density. For each TN, the five most cited publications were included based on the bibliometric analysis results of the SciMAT tool. The data are arranged by level of density. The three themes with the best performance in 2008-2014 were biofuels, biorefinery and biomass, while LCA, biofuels, and biomass stood out in 2015-2018.  Tables 4 and 5. Seven of them (35%) addressed uncertainties analysis aiming to increase the reliability of data obtained with LCA, as emphasized on Baker and Lepech (2009), and some of the studies also cited some uncertainties related sources and specific methods. The highlights for these publications are presented as follow: Cherubini et al. (2009) pointed out the difficulty of completely avoiding uncertainty when applying LCA to bioenergetic systems, because some of the parameters relevant to these systems are not well known, such as nitrous oxide emissions and changes in soil carbon reservoirs; You et al. (2012) suggested further studies to investigate other issues, as uncertainties related to fluctuations in the demand for ethanol, interruptions in biomass supply, emergence of more efficient conversion technologies, and changes in government incentives, among others, that are involved in biofuel supply chains, in order to solidly create and operate these chains; Yue et al. (2014) emphasized the relevance of LCA in assessing the environmental impacts on these chains and highlighted the importance of uncertainty analysis to ensure the reliability of LCA results. In addition to the correlation between sources and types of uncertainty, usually found within the scope of biofuel and bioenergy supply chains, the authors also suggested potential approaches for uncertainties analysis, including scenario analysis, stochastic programming, robust optimization, and fuzzy methods; Čuček et al.(2014) present a multiperiod synthesis and optimization model for integrated biorefinery systems (biofuels) and suggested that this model can improve the uncertainties analysis for these biorefineries; Carneiro et al. (2017) emphasized the relevance of addressing potential uncertainties in the algae biomass supply chain, which stem from various production pathways analyzed, especially when it comes to producing third-generation biofuels, because of this technology's stage of development and the many existing pathway options; Cong et al. (2017) addressed the need to discuss uncertainties related to the option of CH 4 (methane) compared to diesel in the transport sector's and the some examples of types of uncertainty that should be discussed relates to vehicles cost and storage infrastructure in the CH 4 supply chain; and Maes et al. (2015) concluded that when there are uncertainties about the labeling of residue that is added to the process, the results do not reflect a wideranging view of the production chain, and they integrate low impacts regarding toxic emissions and reduce the precision of the allocation of these impacts to the products and coproducts generated. The opposite is true when inflows are labeled clearly as value-added residue. This shows the need to be careful and precise when dealing with different sources and types of uncertainty in material supply pathways, as well as the input parameters of impact assessments. Table 6 presents the other 13 studies (65%), directly related to challenges and approaches for uncertainties analysis in LCA, applied to biorefineries systems and/or their components (e.g., supply chain, economic analysis, environment impact), considering the following characteristics: reference (RF), type of publication (TP), strategy adopted (ST), the approach (AP), and tool used. The main aspects about uncertainties discussions are presented as follow.

From 2008 to 2014
Cherubini and Ulgiati (2010) presented an investigation applying sensitivity analysis for various input parameters and resulting greenhouse gas emissions, measuring the effects of land use changes and corn stover crop residue removal and its use as a raw material in biorefineries. The authors approached this technique with the explicit goal of reviewing the results of the analysis, identifying the parameters ("key parameters") that most influenced the results, and verifying the precision of the data. They concluded that for the case of the LCA study presented in the publication, the most uncertain parameter was the factor used to estimate the amount of carbon reservoir reduction as a result of corn stover removal. This reduction turns to be the key parameter, which strongly affects the final balance of greenhouse gas emissions for a biorefinery system. Cherubini and Jungmeier (2010) applied a sensitivity analysis with same goal addressed by Cherubini and Ulgiati (2010), i.e., review the results, identifying the parameters that most influence the results (change in soil C pools and N 2 O soil emissions), and check the data accuracy. Both studies applied LCA to biorefinery case studies. Floudas et al. (2012) analyze optimization thermochemical process to fuel production from three feedstocks (coal, natural gas, and biomass). The authors present a review about important approaches for these feedstock supply chains, among them are LCA, sensitivity/ uncertainty analysis, and related approaches such as MC, MILP, MINLP, and FP. They also indicate that uncertainty is a "challenge and opportunity" component to be addressed in energy balance processes for feedstock supply chains. Osmani and Zhang (2014) present a review of uncertainty scenarios such as: biomass supply chain, bioethanol (lignocellulosic fuel) demand, feedstock purchase price, and bioenergy product sale price. This research focuses on the financial and environmental performance of the bioethanol feedstock supply chain, in particular, to minimize C emissions. The study also emphasizes that uncertainty scenarios are best evaluated by stochastic methods (e.g., MC) than deterministic methods (e.g., linear optimization models as MILP and MINLP) and presents the results comparing these two approaches and presents a hybrid model (two-stage SMILP). Likewise Cherubini and Ulgiati (2010) and Cherubini and Jungmeier (2010), the authors also applied a sensitivity analysis to verify the influence of uncertainty parameters. Nguyen et al. (2014) focused on the supply chain of the lignocellulosic fuel production, identifying corn stover as the main feedstock at Kansas, USA. The use of MC approach was discussed, by applying Oracle Crystal Ball® tool (Oracle Corporation 2017) for the sensitivity and uncertainty analysis of various uncertainty scenarios around this supply chain on biorefinery systems. In the same way of the previous study, Paap et al. (2013) detailed the strategy to identify the influence of some parameters related to the pretreatment, hydrolysis, and fermentation steps, in the evaluation of uncertain scenarios for a technology under development and commercial scale unproven. The authors evaluated the production process of products derived from switchgrass feedstock and analyzed the influence of these parameters on the production process by using a MC approach in the Crystal Ball® add-in for Microsoft Excel® tool.

From 2015 to 2018
Souza et al. (2015) presented an interesting case study involving an integrated biorefinery system. The study compared the impact assessment results for the traditional ethanol production system, the production of algal biomass-derived biodiesel, and the proposed biorefinery system. Due to the specificities of each system, which could cause inconsistencies in the results, sensitivity and uncertainty analysis was conducted for the input parameters using the traditional MC approach in the Oracle Crystal Ball®. The authors emphasized that the analysis were based on projected performance, which indicates the presence of high levels of uncertainty and must be potentially treated. Farzad et al. (2017) presented a sensitivity analysis related to price parameters aiming to demonstrate the economic viability of installing a biorefinery to produce lignocellulosic sugarcane biofuel. The approach applied for the sensitivity analysis was MC, and the LCA was performed with SimaPro. Boldrin and Astrup (2015) discussed how the uncertainty analysis can be applied to distinct sustainability policies (e.g., European Union Renewable Energy Directive -RED and United States Renewable Fuel Standard -RFS) with  MC approach and SimaPro tool applied to assess uncertain scenarios around the production system of biofuels on biorefineries. Agostinho et al. (2015) adopted an uncertainty analysis with a MC approach through the MC-Simulation Add-In for Microsoft Excel® tool developed by Barreto and Howland (2010). Like the previous studies, the authors performed an uncertainty analysis to assess potential uncertainties in input parameters related to cellulase enzyme industrial production, in this case. In the same way of Osmani and Zhang (2014), the authors briefly discussed the differences between stochastic and deterministic models, to evaluate uncertainty scenarios in LCA studies. Bairamzadeh et al. (2018) presented a robust MILP approach to verify sources and types of uncertainty on lignocellulosic biofuel (bioethanol) supply chain. The authors identified the main sources, types, and uncertainties approaches that can applied in this supply chain through a literature review. Pérez-López et al. (2018) also addressed an analysis around uncertainties parameters related to economic and environmental assessment on a biorefinery system in the same way of You et al.(2012), Yue et al. (2014), Cong et al. (2017), andFarzad et al. (2017). In this study the authors evaluate a microalgal biorefinery to produce renewable fuel and coproducts. Considering that the discussion addressed technologies under development, there were potential uncertainties scenarios to be analyzed. The authors investigated the influence of these uncertainties applying sensitivity and uncertainty analysis, using a MC approach through the @RISK add-in for Microsoft Excel® tool. Gong and You (2017) presented a processing network with over 46,000 alternative production pathways for fuels and value-added products based on algae biomass, indicating a robust mathematical model to support decisions about obtaining fuels and microalgae-based bioproducts with a maximum return on investment (ROI). For a study with this scope, an elevated level of uncertainty in the model's input parameters is to be expected. To process these uncertainties, the authors developed a deterministic model, 2-Stage ARMIFP, in order to identify, within the network and based on the indicated parameters, the optimal pathway that yields the highest ROI. An optimal processing pathway was proposed with the following characteristics: open pond, polyelectrolyte-based flocculation, filtration, high-pressure homogenization, supercritical CO2 extraction, and transesterification catalyzed with sodium methoxide, among others. The model also indicated that the highest productivity would be 25.94%. For future studies, the authors indicated the need for species of microalgae with higher oil productivity and high biomass concentration.

Final Considerations
The present study, which utilized a systematic and bibliometric approach to a literature review, resulted in a broad scientific mapping of how the theme uncertainty analysis is discussed, together with LCA and biorefinery. The results clearly indicate that in the last 10 years, uncertainty analysis has been treated primarily as a way to help validate the results of LCA studies, when it is applied in scenarios that involve multiple sources and different types of uncertainties, as is the case for biorefinery systems. A total of 64 scientific publications came from the WoS and Scopus® databases, and 72.7% of the publications from Scopus® were also listed in the WoS. Thus, the WoS was considered the basis of reference for the computational analysis. The publications included research carried out in the last 10 years and were divided into two time periods: 2008-2014 and 2015-2018. The results were presented both in quantitative (descriptive) and qualitative (content) formats. Regarding the descriptive results, the studies were mostly concentrated in the fields of chemical and biochemical engineering. The journal with the highest number of publications was the Journal of Cleaner Production, edited by Elsevier, which addresses research and practices in sustainability, environment, and cleaner production. In geographical terms, as observed for the authors with the greatest impact factors and most-cited publications, most studies were from the USA, with 44%, followed by Europe, with 42%, while Brazil accounted for 6.3% of the scientific production found in the present study.
Regarding content analysis, the correlations and evolution of the studied themes were investigated through infographics, co-occurrence networks, and strategic keyword diagrams. Graphic analysis showed that in 2008-2014, the theme biorefinery stood out, a concept that gained greater consolidation as a motor theme in the context of bioeconomy. In 2015-2018, the motor theme was LCA, which was strongly correlated to studies about bioenergy, with relevant discussions about the sustainability and viability of fuel and energy production pathways through renewable resources. This study revealed low occurrence of uncertainty analysis in both periods. This low occurrence does not indicate the theme's irrelevance, but only that the studied publications treated uncertainties as a secondary subject, serving to support the validation of results. The results displayed in the graphic analysis were explored by analyzing and discussing the most-cited publications that were related to the main thematic nuclei of each period. The strategy used in this study contributed to a detailed understanding and scientific mapping of various issues and challenges when handling uncertainties in order to minimize risks and errors in environmental impact assessment diagnoses using the LCA technique. It can also help identify the various sources and types of uncertainty, as well as possible approaches to LCA, such as statistical and probabilistic models, and fuzzy methods, among others.
Future studies in the area should include in-depth investigations for the use of advanced probabilistic techniques, such as probabilistic ontologies, to add value to investigations of knowledge representation and reasoning about uncertainties in all stages of LCA studies, especially in the context of complex environments such as biorefinery systems. There are still few guidelines provided in the literature in the area of study of probabilistic ontologies. However, details and discussions in this field of research and yours evolutions have been addressed by Carvalho et al. (2011Carvalho et al. ( , 2013Carvalho et al. ( , 2016Carvalho et al. ( , 2017 and Riali et al. (2019).