Abstract
In the scenario of rising multidrug resistance and allied diseases, antimicrobial peptides can be a considerable alternative for conventional antibiotics due to their broad spectrum activity and ability to bypass common resistance mechanisms. Histone derived antimicrobial peptides are found to have antibacterial, antifungal as well as anticancer properties. Here we have investigated a histone derived peptide sequence from blue swimmer crab, Portunus pelagicus for its general and immunological properties. The Pp-H2A histone derived peptide is an 8.5 kDa peptide with helical conformation on a coiled backbone. Analysis of its physicochemical characteristics such as hydrophobicity, stability and cationicity revealed its suitability as a potential drug candidate possessing antimicrobial function. According to various prediction servers, antibacterial, antifungal, anti-biofilm and anticancer properties could be attributed to the molecule. The study shall serve to strengthen the theoretical framework before proceeding for a time consuming and costly antimicrobial analysis of the molecule.
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Acknowledgement
The authors are grateful to Cochin University of Science and Technology for providing necessary facilities to carry out this work. The first author gratefully acknowledges CSIR (Council of Scientifc & Industrial Research) for the award of a fellowship and the corresponding author to UGC, Government of India for the BSR Faculty Grant (F.18-1/2011(BSR) dt. 16 May 2019). The authors thank Ministry of Earth Sciences (MoES), Govt. of India for the financial assistance (MoES/10-MLR/01/2012) and scientific support for the work. Molecular graphics and analyses performed with UCSF Chimera, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from NIH P41-GM103311
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This work was supported by funding from Ministry of Earth Sciences (MoES), Government of India (MoES/10-MLR/01/2012). The first author received research support from the Council of Scientific and Industrial Research (CSIR) (09/239(0566)/2020-EMR-I) and the corresponding author from UGC, Government of India for the BSR Faculty Grant (F.18-1/2011(BSR) dt. 16 May 2019).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Anooja V V with the support of Athira P P, Dhanya Kesavan, Neelima S, Anju M V, Archana K, Muhammed Musthafa S. The work was carried out under the supervision of Dr. Rosamma Philip and Dr. C K Radhakrishnan. The manuscript was written by Anooja V V and corrected by Dr. Rosamma Philip.
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Anooja, V.V., Athira, P.P., Dhanya, K. et al. A Histone H2A Derived Antimicrobial Peptide from the Blue Swimmer Crab, Portunus pelagicus Linnaeus 1758: In silico Characterization and Phylogenetic Analysis. Thalassas 40, 65–79 (2024). https://doi.org/10.1007/s41208-023-00607-9
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DOI: https://doi.org/10.1007/s41208-023-00607-9