Abstract
Among different strategies for the treatment of bone-related abnormalities, bone regeneration or replacement has been accepted as most successful. However, much attention is required for the creation of artificial bone implants. Although there are still many obstacles to overcome before safe and dependable clinical applications are realized, remarkable progress in tissue engineering has showed considerable promise in bone repair. This review explores the potential of graphene and its derivatives as superior scaffolds in the field of bone tissue engineering, highlighting their adaptable physical, chemical, and biological properties. A primer on the characteristics of graphene and its analogues is presented here with further fascinating advancement and challenges in bone tissue engineering with focus on their applicability in fabricating bone support system.
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References
Langer R, Vacanti JP (1993) Tissue engineering. Science 260:920–926
Habibovic P (2017) Strategic directions in osteoinduction and biomimetics. Tissue Eng Part A 23:1295–1296
Perez JR, Kouroupis D, Li DJ, et al (2018) Tissue engineering and cell-based therapies for fractures and bone defects. Front Bioeng Biotechnol 6
Clynes MA, Harvey NC, Curtis EM et al (2020) The epidemiology of osteoporosis. Br Med Bull 133:105–117
Arvidson K, Abdallah BM, Applegate LA et al (2011) Bone regeneration and stem cells. J Cell Mol Med 15:718–746
Wang P, Zhao L, Liu J et al (2014) Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells. Bone Res 2:1–13
Willie BM, Petersen A, Schmidt-Bleek K et al (2010) Designing biomimetic scaffolds for bone regeneration: why aim for a copy of mature tissue properties if nature uses a different approach? Soft Matter 6:4976
Killington K, Mafi R, Mafi P et al (2018) A systematic review of clinical studies investigating mesenchymal stem cells for fracture non-union and bone defects. Curr Stem Cell Res Ther 13:284–291
Fuchs JR, Nasseri BA, Vacanti JP (2001) Tissue engineering: a 21st century solution to surgical reconstruction. Ann Thorac Surg 72:577–591
Saltzman WM, Saltzman WM (2004) Tissue engineering: engineering principles for the design of replacement organs and tissues. Oxford University Press, Oxford
Fu Q, Saiz E, Rahaman MN et al (2011) Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. Mater Sci Eng C 31:1245–1256
Asnaghi A, Macchiarini P, Mantero S (2009) Tissue engineering toward organ replacement: a promising approach in airway transplant. Int J Artif Organs 32:763–768
Haas NP (2000) Callusmodulation – Fiktion oder Realität? Chir 71:987–988
Minas T, Peterson L (1999) Advanced techniques in autologous chondrocyte transplantation. Clin Sports Med 18:13–44
Wu G-H, Hsu S (2015) Review: polymeric-based 3D printing for tissue engineering. J Med Biol Eng 35:285–292
Sah MK, Rath SN (2016) Soluble eggshell membrane: a natural protein to improve the properties of biomaterials used for tissue engineering applications. Mater Sci Eng C 67:807–821
Aggarwal A, Sah MK (2021) Chapter Three: Electrospun materials as scaffolds in tissue engineering and regenerative medicine, In: Kasoju N, Ye H (eds), Biomedical applications of electrospinning and electrospraying, Woodhead Publishing, 83–121
Spain Tl, Agrawal CM, Athanasiou KA (1998) New technique to extend the useful life of a biodegradable cartilage implant. Tissue Eng 4:343–352
Ghorbel H, Guidara A, Guidara R et al (2020) Assessment of the addition of Fluorapatite-alumina coating for a durable adhesion of the interface prosthesis/bone cells: implementation in vivo. J Med Biol Eng 40:158–168
Filippi M, Born G, Chaaban M, et al. (2020) Natural polymeric scaffolds in bone regeneration. Front Bioeng Biotechnol 8
Oladeji LO, Stannard JP, Cook CR et al (2017) Effects of autogenous bone marrow aspirate concentrate on radiographic integration of femoral condylar osteochondral allografts. Am J Sports Med 45:2797–2803
Du Z, Feng X, Cao G et al (2021) The effect of carbon nanotubes on osteogenic functions of adipose-derived mesenchymal stem cells in vitro and bone formation in vivo compared with that of nano-hydroxyapatite and the possible mechanism. Bioact Mater 6:333–345
Wang Q, Yan J, Yang J et al (2016) Nanomaterials promise better bone repair. Mater Today 19:451–463
Du Z, Wang C, Zhang R et al (2020) Applications of graphene and its derivatives in bone repair: advantages for promoting bone formation and providing real-time detection, challenges and future prospects. Int J Nanomed 15:7523–7551
Eivazzadeh-Keihan R, Maleki A, de la Guardia M et al (2019) Carbon based nanomaterials for tissue engineering of bone: building new bone on small black scaffolds: a review. J Adv Res 18:185–201
Li G, Zhou T, Lin S et al (2017) Nanomaterials for craniofacial and dental tissue engineering. J Dent Res 96:725–732
Katz JL, Meunier A (1987) The elastic anisotropy of bone. J Biomech 20:1063–1070
Rho J-Y, Kuhn-Spearing L, Zioupos P (1998) Mechanical properties and the hierarchical structure of bone. Med Eng Phys 20:92–102
Cheng J, Liu J, Wu B, et al. (2021) Graphene and its derivatives for bone tissue engineering: in vitro and in vivo evaluation of graphene-based scaffolds, membranes and coatings. Front Bioeng Biotechnol 9
Ho-Shui-Ling A, Bolander J, Rustom LE et al (2018) Bone regeneration strategies: engineered scaffolds, bioactive molecules and stem cells current stage and future perspectives. Biomaterials 180:143–162
Iaquinta MR, Mazzoni E, Manfrini M et al (2019) Innovative biomaterials for bone regrowth. Int J Mol Sci 20:618
Rahat Rahman M, Rashid MdM, Islam MdM et al (2019) Electrical and chemical properties of graphene over composite materials: a technical review. Mater Sci Res India 16:142–163
Lee C, Wei X, Kysar JW et al (2008) Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 321:385–388
Young RJ, Kinloch IA, Gong L et al (2012) The mechanics of graphene nanocomposites: a review. Compos Sci Technol 72:1459–1476
Gao C, Feng P, Peng S et al (2017) Carbon nanotube, graphene and boron nitride nanotube reinforced bioactive ceramics for bone repair. Acta Biomater 61:1–20
Trusek A, Kijak E, Granicka L (2020) Graphene oxide as a potential drug carrier: chemical carrier activation, drug attachment and its enzymatic controlled release. Mater Sci Eng C 116:111240
Chung C, Kim Y-K, Shin D et al (2013) Biomedical applications of graphene and graphene oxide. Acc Chem Res 46:2211–2224
Loh KP, Bao Q, Eda G et al (2010) Graphene oxide as a chemically tunable platform for optical applications. Nat Chem 2:1015–1024
Park S, Ruoff RS (2009) Chemical methods for the production of graphenes. Nat Nanotechnol 4:217–224
Smith AT, LaChance AM, Zeng S et al (2019) Synthesis, properties, and applications of graphene oxide/reduced graphene oxide and their nanocomposites. Nano Mater Sci 1:31–47
Priyadarsini S, Mohanty S, Mukherjee S et al (2018) Graphene and graphene oxide as nanomaterials for medicine and biology application. J Nanostruct Chem 8:123–137
Dash BS, Jose G, Lu Y-J et al (2021) Functionalized reduced graphene oxide as a versatile tool for cancer therapy. Int J Mol Sci 22:2989
Yuan Q, Lin C-T, Chee KWA (2019) All-carbon devices based on sp2-on-sp3 configuration. APL Mater 7:030901
Marcano DC, Kosynkin DV, Berlin JM et al (2010) Improved synthesis of graphene oxide. ACS Nano 4:4806–4814
Rao CNR, Sood AK, Subrahmanyam KS et al (2009) Graphene: the new two-dimensional nanomaterial. Angew Chem Int Ed 48:7752–7777
Shin YC, Bae J-H, Lee JH et al (2022) Enhanced osseointegration of dental implants with reduced graphene oxide coating. Biomater Res 26:11
Du X, Skachko I, Barker A et al (2008) Approaching ballistic transport in suspended graphene. Nat Nanotechnol 3:491–495
Gómez-Navarro C, Weitz RT, Bittner AM et al (2007) Electronic transport properties of individual chemically reduced graphene oxide sheets. Nano Lett 7:3499–3503
Karki N, Tiwari H, Tewari C et al (2020) Functionalized graphene oxide as a vehicle for targeted drug delivery and bioimaging applications. J Mater Chem B 8:8116–8148
Hu M, Mi B (2014) Layer-by-layer assembly of graphene oxide membranes via electrostatic interaction. J Membr Sci C, 80–87
Medhekar NV, Ramasubramaniam A, Ruoff RS et al (2010) Hydrogen bond networks in graphene oxide composite paper: structure and mechanical properties. ACS Nano 4:2300–2306
Zhang N, Cong X (2018) Enhanced nonlinear absorption performance of reduced graphene oxide nanohybrid covalently functionalized by porphyrin via 1,3-dipolar cycloaddition. Mater Sci Appl 9:972–984
Song P, Xu Z, Wu Y et al (2017) Super-tough artificial nacre based on graphene oxide via synergistic interface interactions of π–π stacking and hydrogen bonding. Carbon 111:807–812
Cheng J, Liu J, Wu B et al (2021) Graphene and its derivatives for bone tissue engineering: In vitro and in vivo evaluation of graphene-based scaffolds, membranes and coatings. Front Bioeng Biotechnol 9:734688
Shin SR, Li Y-C, Jang HL et al (2016) Graphene-based materials for tissue engineering. Adv Drug Deliv Rev 105:255–274
Daneshmandi L, Barajaa M, Tahmasbi Rad A et al (2021) Graphene-based biomaterials for bone regenerative engineering: a comprehensive review of the field and considerations regarding biocompatibility and biodegradation. Adv Healthc Mater 10:2001414
Banerjee AN (2018) Graphene and its derivatives as biomedical materials: future prospects and challenges. Interface Focus 8:20170056
Cheng X, Wan Q, Pei X (2018) Graphene family materials in bone tissue regeneration: perspectives and challenges. Nanoscale Res Lett 13:289
Hermenean A, Codreanu A, Herman H et al (2017) Chitosan-graphene oxide 3D scaffolds as promising tools for bone regeneration in critical-size mouse calvarial defects. Sci Rep 7:16641
Nayak TR, Andersen H, Makam VS et al (2011) Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 5:4670–4678
Shadjou N, Hasanzadeh M (2016) Graphene and its nanostructure derivatives for use in bone tissue engineering: recent advances. J Biomed Mater Res A 104:1250–1275
Georgakilas V, Tiwari JN, Kemp KC et al (2016) Noncovalent functionalization of graphene and graphene oxide for energy materials, biosensing, catalytic, and biomedical applications. Chem Rev 116:5464–5519
Bahrami S, Baheiraei N, Shahrezaee M (2021) Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration. Sci Rep 11:16783
Pei B, Wang W, Dunne N et al (2019) Applications of carbon nanotubes in bone tissue regeneration and engineering: Superiority, concerns, current advancements, and prospects. Nanomaterials 9:1501
Bruschi A, Donati DM, Choong P et al (2021) Dielectric elastomer actuators, neuromuscular interfaces, and foreign body response in artificial neuromuscular prostheses: a review of the literature for an in vivo application. Adv Healthc Mater 10:2100041
Driscoll J, Moirangthem A, Yan IK, et al (2021) Fabrication and characterization of a biomaterial based on extracellular-vesicle functionalized graphene oxide. Front Bioeng Biotechnol 9
Gu M, Liu Y, Chen T et al (2014) Is graphene a promising nano-material for promoting surface modification of implants or scaffold materials in bone tissue engineering? Tissue Eng Part B Rev 20:477–491
Pahlevanzadeh F, Ebrahimian-Hosseinabadi M (2019) Poly (methyl methacrylate)/biphasic calcium phosphate/nano graphene bone cement for orthopedic application. J Med Signals Sens 9:33–41
Chen J, Zhang X, Cai H et al (2016) Osteogenic activity and antibacterial effect of zinc oxide/carboxylated graphene oxide nanocomposites: preparation and in vitro evaluation. Colloids Surf B Biointerfaces 147:397–407
Kaur T, Thirugnanam A, Pramanik K (2017) Effect of carboxylated graphene nanoplatelets on mechanical and in-vitro biological properties of polyvinyl alcohol nanocomposite scaffolds for bone tissue engineering. Mater Today Commun 12:34–42
Tavafoghi M, Brodusch N, Gauvin R et al (2016) Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid. J R Soc Interface 13:20150986
Paz E, Ballesteros Y, Forriol F et al (2019) Graphene and graphene oxide functionalisation with silanes for advanced dispersion and reinforcement of PMMA-based bone cements. Mater Sci Eng C 104:109946
Vuppaladadium SSR, Agarwal T, Kulanthaivel S et al (2020) Silanization improves biocompatibility of graphene oxide. Mater Sci Eng C 110:110647
Sun J, Deng Y, Li J et al (2016) A new graphene derivative: hydroxylated graphene with excellent biocompatibility. ACS Appl Mater Interfaces 8:10226–10233
Lu J, Cheng C, He Y-S et al (2016) Multilayered graphene hydrogel membranes for guided bone regeneration. Adv Mater Deerfield Beach Fla 28:4025–4031
Cheng J, Liu H, Zhao B et al (2015) MC3T3-E1 preosteoblast cell-mediated mineralization of hydroxyapatite by poly-dopamine-functionalized graphene oxide. J Bioact Compat Polym 30:289–301
Jia Z, Shi Y, Xiong P et al (2016) From solution to biointerface: graphene self-assemblies of varying lateral sizes and surface properties for biofilm control and osteodifferentiation. ACS Appl Mater Interfaces 8:17151–17165
Padmavathy N, Jaidev LR, Bose S et al (2017) Oligomer-grafted graphene in a soft nanocomposite augments mechanical properties and biological activity. Mater Des 126:238–249
Sharma R, Kapusetti G, Bhong SY et al (2017) Osteoconductive amine-functionalized graphene–poly(methyl methacrylate) bone cement composite with controlled exothermic polymerization. Bioconjug Chem 28:2254–2265
Liu X, Ma D, Tang H et al (2014) Polyamidoamine dendrimer and oleic acid-functionalized graphene as biocompatible and efficient gene delivery vectors. ACS Appl Mater Interfaces 6:8173–8183
Dou C, Ding N, Luo F et al (2018) Graphene-based MicroRNA transfection blocks preosteoclast fusion to increase bone formation and vascularization. Adv Sci 5:1700578
Eckhart KE, Holt BD, Laurencin MG et al (2019) Covalent conjugation of bioactive peptides to graphene oxide for biomedical applications. Biomater Sci 7:3876–3885
Li K, Zhang Z, Li D et al (2018) Biomimetic ultralight, highly porous, shape-adjustable, and biocompatible 3D graphene minerals via incorporation of self-assembled peptide nanosheets. Adv Funct Mater 28:1801056
Kang E-S, Kim D-S, Han Y et al (2018) Three-dimensional graphene–RGD peptide nanoisland composites that enhance the osteogenesis of human adipose-derived mesenchymal stem cells. Int J Mol Sci 19:669
Kim K-H, No Y-S (2017) Subwavelength core/shell cylindrical nanostructures for novel plasmonic and metamaterial devices. Nano Converg 4:32
Zhang W, Yang G, Wang X et al (2017) Magnetically controlled growth-factor-immobilized multilayer cell sheets for complex tissue regeneration. Adv Mater 29:1703795
Yao Q, Liu Y, Sun H (2018) Heparin–dopamine functionalized graphene foam for sustained release of bone morphogenetic protein-2. J Tissue Eng Regen Med 12:1519–1529
Rajan Unnithan A, Ramachandra Kurup Sasikala A, Park CH et al (2017) A unique scaffold for bone tissue engineering: An osteogenic combination of graphene oxide–hyaluronic acid–chitosan with simvastatin. J Ind Eng Chem 46:182–191
Sun H, Zhang L, Xia W et al (2016) Fabrication of graphene oxide-modified chitosan for controlled release of dexamethasone phosphate. Appl Phys A 122:632
Liang C, Luo Y, Yang G et al (2018) Graphene oxide hybridized nHAC/PLGA scaffolds facilitate the proliferation of MC3T3-E1 cells. Nanoscale Res Lett 13:15
Weng W, Nie W, Zhou Q et al (2017) Controlled release of vancomycin from 3D porous graphene-based composites for dual-purpose treatment of infected bone defects. RSC Adv 7:2753–2765
Qiu J, Guo J, Geng H et al (2017) Three-dimensional porous graphene nanosheets synthesized on the titanium surface for osteogenic differentiation of rat bone mesenchymal stem cells. Carbon 125:227–235
Lyu H, He Z, Chan YK et al (2019) Hierarchical ZnO nanotube/graphene oxide nanostructures endow pure Zn implant with synergistic bactericidal activity and osteogenicity. Ind Eng Chem Res 58:19377–19385
Shahin M, Munir K, Wen C et al (2020) Magnesium-based composites reinforced with graphene nanoplatelets as biodegradable implant materials. J Alloys Compd 828:154461
Zhao Y, Chen J, Zou L et al (2019) Facile one-step bioinspired mineralization by chitosan functionalized with graphene oxide to activate bone endogenous regeneration. Chem Eng J 378:122174
Oğuz ÖD, Ege D (2019) Preparation of graphene oxide-reinforced calcium phosphate/calcium sulfate/methylcellulose-based injectable bone substitutes. MRS Commun 9:1174–1180
Dhavale VM, Singh SK, Nadeema A et al (2015) Nanocrystalline Fe–Fe2O3 particle-deposited N-doped graphene as an activity-modulated Pt-free electrocatalyst for oxygen reduction reaction. Nanoscale 7:20117–20125
Li J, Jiang H, Ouyang X et al (2016) CaCO3/tetraethylenepentamine–graphene hollow microspheres as biocompatible bone drug carriers for controlled release. ACS Appl Mater Interfaces 8:30027–30036
Tang J, Cao W, Zhang Y et al (2019) Properties of vaterite-containing tricalcium silicate composited graphene oxide for biomaterials. Biomed Mater 14:045004
Dai C, Li Y, Pan W et al (2020) Three-dimensional high-porosity chitosan/honeycomb porous carbon/hydroxyapatite scaffold with enhanced osteoinductivity for bone regeneration. ACS Biomater Sci Eng 6:575–586
Li J, Liu X, Tomaskovic-Crook E et al (2019) Smart graphene-cellulose paper for 2D or 3D “origami-inspired” human stem cell support and differentiation. Colloids Surf B Biointerfaces 176:87–95
Liu S, Zhou C, Mou S et al (2019) Biocompatible graphene oxide–collagen composite aerogel for enhanced stiffness and in situ bone regeneration. Mater Sci Eng C 105:110137
Zhang D, Wu X, Chen J et al (2018) The development of collagen based composite scaffolds for bone regeneration. Bioact Mater 3:129–138
Unagolla JM, Jayasuriya AC (2019) Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds. Mater Sci Eng C 102:1–11
Bhusari SA, Sharma V, Bose S et al (2019) HDPE/UHMWPE hybrid nanocomposites with surface functionalized graphene oxide towards improved strength and cytocompatibility. J R Soc Interface 16:20180273
Feng Z, Li Y, Hao L et al (2019) Graphene-Reinforced Biodegradable Resin Composites for Stereolithographic 3D Printing of Bone Structure Scaffolds. J Nanomater 2019:e9710264
Liu C, Wong HM, Yeung KWK et al (2016) Novel electrospun polylactic acid nanocomposite fiber mats with hybrid graphene oxide and nanohydroxyapatite reinforcements having enhanced biocompatibility. Polymers 8:287
Mahdavi R, Belgheisi G, Haghbin-Nazarpak M et al (2020) Bone tissue engineering gelatin–hydroxyapatite/graphene oxide scaffolds with the ability to release vitamin D: fabrication, characterization, and in vitro study. J Mater Sci Mater Med 31:97
Dalgic AD, Alshemary AZ, Tezcaner A et al (2018) Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering. J Biomater Appl 32:1392–1405
Lee JH, Shin YC, Lee S-M et al (2015) Enhanced osteogenesis by reduced graphene oxide/hydroxyapatite nanocomposites. Sci Rep 5:18833
Purohit SD, Bhaskar R, Singh H et al (2019) Development of a nanocomposite scaffold of gelatin–alginate–graphene oxide for bone tissue engineering. Int J Biol Macromol 133:592–602
Zhou K, Yu P, Shi X et al (2019) Hierarchically porous hydroxyapatite hybrid scaffold incorporated with reduced graphene oxide for rapid bone ingrowth and repair. ACS Nano 13:9595–9606
Wang W, Caetano G, Ambler WS et al (2016) Enhancing the hydrophilicity and cell attachment of 3D printed PCL/graphene scaffolds for bone tissue engineering. Materials 9:992
Wang W, Junior JRP, Nalesso PRL et al (2019) Engineered 3D printed poly(ɛ-caprolactone)/graphene scaffolds for bone tissue engineering. Mater Sci Eng C 100:759–770
Șelaru A, Herman H, Vlăsceanu GM et al (2022) Graphene-oxide porous biopolymer hybrids enhance in vitro osteogenic differentiation and promote ectopic osteogenesis in vivo. Int J Mol Sci 23:491
Yang Z, Liu J, Liu J et al (2021) Investigation on physicochemical properties of graphene oxide/nano-hydroxyapatite composites and its biomedical applications. J Aust Ceram Soc 57:625–633
Nosrati H, Le DQS, Emameh RZ, et al (2019) Characterization of the precipitated dicalcium phosphate dehydrate on the graphene oxide surface as a bone cement reinforcement
Saravanan S, Chawla A, Vairamani M et al (2017) Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo. Int J Biol Macromol 104:1975–1985
Wu X, Zheng S, Ye Y et al (2018) Enhanced osteogenic differentiation and bone regeneration of poly(lactic-co-glycolic acid) by graphene via activation of PI3K/Akt/GSK-3β/β-catenin signal circuit. Biomater Sci 6:1147–1158
Kolanthai E, Sindu PA, Khajuria DK et al (2018) Graphene oxide—a tool for the preparation of chemically crosslinking free alginate–chitosan–collagen scaffolds for bone tissue engineering. ACS Appl Mater Interfaces 10:12441–12452
Chu J, Shi P, Yan W et al (2018) PEGylated graphene oxide-mediated quercetin-modified collagen hybrid scaffold for enhancement of MSCs differentiation potential and diabetic wound healing. Nanoscale 10:9547–9560
Guo W, Wang S, Yu X et al (2016) Construction of a 3D rGO–collagen hybrid scaffold for enhancement of the neural differentiation of mesenchymal stem cells. Nanoscale 8:1897–1904
Zhou C, Liu S, Li J et al (2018) Collagen functionalized with graphene oxide enhanced biomimetic mineralization and in situ bone defect repair. ACS Appl Mater Interfaces 10:44080–44091
Yang K, Li Y, Tan X et al (2013) Behavior and toxicity of graphene and its functionalized derivatives in biological systems. Small 9:1492–1503
Iannazzo D, Pistone A, Ziccarelli I et al (2018) Chapter 8: Graphene-based materials for application in pharmaceutical nanotechnology. In: Grumezescu AM (ed) Fullerens. William Andrew Publishing, Graphenes and Nanotubes, pp 297–329
Duch MC, Budinger GRS, Liang YT et al (2011) Minimizing oxidation and stable nanoscale dispersion improves the biocompatibility of graphene in the lung. Nano Lett 11:5201–5207
Tahriri M, Del Monico M, Moghanian A et al (2019) Graphene and its derivatives: opportunities and challenges in dentistry. Mater Sci Eng C 102:171–185
Liu Z, Davis C, Cai W et al (2008) Circulation and long-term fate of functionalized, biocompatible single-walled carbon nanotubes in mice probed by Raman spectroscopy. Proc Natl Acad Sci 105:1410–1415
Dasari Shareena TP, McShan D, Dasmahapatra AK et al (2018) A review on graphene-based nanomaterials in biomedical applications and risks in environment and health. Nano-Micro Lett 10:53
Geetha Bai R, Muthoosamy K, Manickam S et al (2019) Graphene-based 3D scaffolds in tissue engineering: fabrication, applications, and future scope in liver tissue engineering. Int J Nanomedicine 14:5753–5783
Ruiz ON, Fernando KAS, Wang B et al (2011) Graphene oxide: a nonspecific enhancer of cellular growth. ACS Nano 5:8100–8107
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The authors express their gratitude to the Ministry of Education (MoE), Government of India, for awarding the scholarship to the initial two authors.
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Handa, S., Dan, S. & Sah, M.K. Graphene and its derivatives as support system ingredient for bone fracture repair. Graphene and 2D mater 8, 43–58 (2023). https://doi.org/10.1007/s41127-023-00060-8
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DOI: https://doi.org/10.1007/s41127-023-00060-8