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Antimycobacterial, Anticancer, Antioxidant and Photocatalytic Activity of Biosynthesized Silver Nanoparticles Using Berberis Integerrima

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Abstract

Berberis integerrima with various secondary metabolites is used in the treatment of various diseases. In this project, silver nanoparticles (AgNPs) were prepared through a green process using a fruit extract of B. integerrima. Several techniques such as UV–Vis absorption spectroscopy, TEM, PXRD, FTIR and zeta potential analysis were used in characterization of biosynthesized AgNPs. The biosynthesized AgNPs were in spherically shaped with a mean diameter of 30 nm. Moreover, the biosynthesized AgNPs seemed to exhibit a higher bactericidal and antifungal activity against infective bacteria (S. aureu s, E. coli, P. aeruginosa) and pathogendic fungi (Candida species). Also, green fabricated nanoparticles show suitable photocatalytic activity that AgNPs destroyed up to 82.5% of the methylene blue dye from aqueous environment. Antioxidant test based on total phenolic compounds showed the content of total phenol in the B. integerrima extract and AgNPs was 51 and 21 mg gallic acid equivalent, respectively. The anticancer effect against human liver cancer cell line (HepG2) showed that high anticancer effects depend on the time and concentration of extract and AgNPs. These results suggest that the these nanoparticles could be efficiently apply as an alternate antimicrobial/anticancer agent in contrast to infections and cancerous cell lines, and is beneficial for purification of water and pollutants solutions in environment.

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Abbreviations

TEM:

Transmission electron microscopy

PXRD:

Powder X-ray diffraction

FTIR:

Fourier transform infrared spectroscopy

AgNPs:

Silver nanoparticles

DMEM:

Dulbecco's modified Eagle's medium

FBS:

Fetal bovine serum

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

SPR:

Surface plasmon resonant

MIC:

Minimal reticence concentrations

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Acknowledgements

This project was performed with the support of the Mashhad University of Medical Sciences (Grant no. 971634) funded by Iranian government, Islamic Republic of Iran.

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Authors and Affiliations

  1. Medical Toxicology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Ehsan Taghavizadeh Yazdi, Hamid Mozafarri & Seyed Hadi Mousavi

  2. Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Majid Darroudi

  3. Department of Biology, Payame Noor University, Tehran, Iran

    Mohammad Sadegh Amiri

  4. Allergy Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Hossein Zarrinfar

  5. Chemistry Department, Payame Noor University, 19395-4697, Tehran, Iran

    Hasan Ali Hosseini

  6. Pharmaceutical Technology Institute, Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Mohammad Mashreghi

  7. Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, 91779-44553, Mashhad, Iran

    Ahmad Ghorbani

  8. Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Seyed Hadi Mousavi

Authors
  1. Mohammad Ehsan Taghavizadeh Yazdi
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  2. Majid Darroudi
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  3. Mohammad Sadegh Amiri
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  4. Hossein Zarrinfar
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  5. Hasan Ali Hosseini
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  6. Mohammad Mashreghi
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  7. Hamid Mozafarri
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  8. Ahmad Ghorbani
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  9. Seyed Hadi Mousavi
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Correspondence to Seyed Hadi Mousavi.

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Taghavizadeh Yazdi, M.E., Darroudi, M., Amiri, M.S. et al. Antimycobacterial, Anticancer, Antioxidant and Photocatalytic Activity of Biosynthesized Silver Nanoparticles Using Berberis Integerrima. Iran J Sci Technol Trans Sci 46, 1–11 (2022). https://doi.org/10.1007/s40995-021-01226-w

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  • DOI: https://doi.org/10.1007/s40995-021-01226-w

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