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Damage quantification in Physalis peruviana L. infected by the new putative sobemovirus physalis rugose mosaic virus

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Abstract

A putative new virus, physalis rugose mosaic virus (PhyRMV), has been reported more frequently in Physalis peruviana associated with severe symptoms. In this study the damage caused by sap-inoculated PhyRMV in P. peruviana was assessed based on the comparison of host vegetative and reproductive parameters between virus-infected and healthy plants. Infected plants confirmed using molecular assays, showed a reduction in growth, leaf area, specific leaf area (SLA) and relative chlorophyll content. PhyRMV-infected plants yielded 70% less fruit of general lower quality parameters, except for pH, compared with the healty plants. Finally, seeds from PhyRMV-infected plants showed a reduced germination rate and, in accelerated aging assay, vigor of seeds were also significantly reduced.

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Acknowledgements

This study was funded by Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina (FAPESC- PROC619/2017) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq – 437059/2018-9). The authors thank Dr. Altamir Frederico Guidolin for the use of his laboratory and supply of some reagents. The authors are also grateful to two anonymous referees and the editors for suggestions to improve the readability of the manuscript.

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Authors and Affiliations

  1. Universidade do Estado de Santa Catarina, Lages, Santa Catarina, 88520-000, Brazil

    Eduardo Silva Gorayeb, Amanda Savi, Mayra Juline Gonçalves, Cileide Maria Medeiros de Souza, Amauri Bogo, Ricardo Trezzi Casa & Fabio Nascimento da Silva

  2. Instituto Federal Catarinense, Rio do Sul, Santa Catarina, 89163-356, Brazil

    Clenilso Sehnen Mota

  3. Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, 96160-000, Brazil

    Danielle Ribeiro de Barros

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  1. Eduardo Silva Gorayeb
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  2. Amanda Savi
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  3. Mayra Juline Gonçalves
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  4. Clenilso Sehnen Mota
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  7. Amauri Bogo
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  9. Fabio Nascimento da Silva
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Contributions

Design of the study: FNS, EGS, AS. Conducting experiments: EGS, AS, MJG, CSM. Statistical analysis: EGS, AS. Data interpretation: FNS, EGS, AS, CMMS, CSM. Paper preparation: FNS, EGS, AS. Contributed reagents and materials: FNS, CMMS, DRB, AB, RTC. Critical paper review: FNS, EGS, AS, MJG, CSM, CMMS, DRB, AB, RTC. Language editing: FNS, ESG, AB. All authors discussed the results and contributed to the final manuscript.

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Correspondence to Fabio Nascimento da Silva.

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Section Editor: Juliana Freitas-Astua

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Gorayeb, E.S., Savi, A., Gonçalves, M.J. et al. Damage quantification in Physalis peruviana L. infected by the new putative sobemovirus physalis rugose mosaic virus. Trop. plant pathol. 45, 476–483 (2020). https://doi.org/10.1007/s40858-020-00354-9

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