Plant growth and their root development after inoculation of arbuscular mycorrhizal fungi in coal mine subsided areas

Coal mining often cause serious land degradation, soil erosion, and desertiﬁcation affecting growth of the local vegetation, especially the roots. Arbuscular mycorrhizal fungi (AMF) inoculation is considered a potential biotechnological tool for mined soil remediation because mycorrhizal fungi could improve plant growth environment, especially under adverse conditions due to their good symbiosis. A ﬁeld experiment was conducted to study the ecological effects of AMF ( Funneliformis mosseae , Rhizophagus intraradices ) on the growth of Amygdalus pedunculata Pall. and their root development in the regenerated mining subsidence sandy land. The reclamation experiment included four treatments: inoculation of Funneliformis mosseae (F.m), inoculation of Rhizophagus intraradices (R.i), combined inoculation of F.m and R.i and non-inoculated treatment. Root mycorrhizal colonization, plant height, crown width, soil moisture, root morphology and certain soil properties were assessed. The results showed that AMF improved the shoot and root growth of Amygdalus pedunculata Pall., and signiﬁcantly increased root colonization after 1 year of inoculation. Available phosphorus content, activities of phosphatase as well as electrical conductivity in soil rhizosphere of all the three inoculation treatments were higher than that of the non-inoculated treatment. AMF increased the quantity of bacteria and fungi in soil rhizosphere compared with the non-inoculated treatment. Our study indicates that revegetation with AMF inoculum could inﬂuence plant growth and root development as well as soil properties, suggesting that AMF inoculation can be effective method for further ecological restoration in coal mine subsided areas.


Introduction
China is the world's largest coal producing and consuming country (Li et al. 2011). Although coal brings huge economic benefits to China, coal mining causes numerous environmental problems, such as drastic reduction in the number of biological communities in the reconstructed soil induced by any disturbance in coal mining, plant root damage caused by mining subsidence, reduced topsoil fertility, water-fertilizer disharmony in soil, and pollution by filling materials (Zang et al. 2010;Tripathi et al. 2009). Simultaneously, the vegetation coverage is gradually decreasing and thus severely restricts the sustainable development of eco-environment in Western China. Previous studies demonstrated that microbial activity is a key factor affecting the functioning of all terrestrial systems (Quadros et al. 2016).
Soil microbiota communities play crucial role in maintaining the soil ecological balance as well as the sustainability of both natural ecosystem and agro-ecosystem. Beneficial rhizospheric microbe-plant interactions have a great influence on plant health and soil quality (Wu et al. 2008). Among these beneficial rhizospheric microbes, arbuscular mycorrhizal fungi (AMF) and plant-growth promoting rhizobacteria (PGPR) can be considered. Arbuscular mycorrhizal fungi are obligate symbionts that colonise the roots of most cultivated plant species. Mycorrhizal symbiosis can be found in nearly all types of ecological situations and most plant species are able to form this symbiosis naturally (Gong et al. 2012). These associations occur naturally when plantlets are transplanted into the field, favouring plant development by increasing nutrient uptake, growth rates and hormonal activities (del Carmen Jaizme-Vega et al. 2006;Chandanie et al. 2009). Mycorrhizae may also increase plant tolerance to stress conditions such as salinity and drought (Latef and He 2011), heavy metals (Tian et al. 2013), root soil borne pathogens (Miller and Jastrow 1992) and the improvement of soil structure (Matthiasc et al. 2010).
Amygdalus pedunculata Pall. is a native tree specie with quick adaptability and high economic and ecological value, which is one of the economic plants used as ecological restoration pioneering species in Shendong coal mine areas (Ma et al. 2006). Studies concerning Amygdalus pedunculata Pall. mainly focus on the physiological and ecological characteristics, plant breeding methods, resources development and utilization, etc., mechanisms and effects of AMF on it has rarely been studied (Ma et al. 2006;Xu and Chu 2013;Chen et al. 2013;Cao et al. 2016). In this study, the impact of two Glomus species on the plant growth and their root development in coal mining subsided land were studied.

Study site description
The study was conducted at Daliuta mining area located at the transition zone between Loess Plateau in Northern Shaanxi and the Mu Us Desert. Its altitude ranges from 1000 to 1250 m and has a semiarid continental monsoon climate. The average annual temperature is about 8.4°C with the highest temperature of 38°C and the lowest of -28°C. Mean annual precipitation is 441.2 mm, which is concentrated between June and September. Annual average evaporation is 2211.2 mm. The area has thick loosened aquifer, and the soil is sandy (Bi and Zhang 2014).

Experimental materials
Two AM fungi were used in the study. Funneliformis mosseae (F.m)was cultured under maize with 90% of root colonisation, and Rhizophagus intraradices(R.i)was cultured under maize with 84% of root colonization. They are two kinds of AMF with wide application and strong ability to adapt to the coal mine environment. The tested plant is Amygdalus pedunculata Pall. under field conditions. They were planted in April 2012. For the inoculation treatments, 50 g of sagent were inoculated near by the root after digging out the root carefully in the following July at the beginning of the nursery phase. After microbial inoculants were evenly applied on the plants, soil was filled back and compacted.

Experimental design
The experiment was conducted by a completely random design method to 4 treatments: (1) non-inoculated as the control treatment(CK); (2) single inoculation of F.m, (3) single inoculation of R.i, and (4) combined inoculation of F.m and R.i. The soil samples were collected in September 2013, 1 year after inoculation. Each treatment was replicated ten times.

Experimental methods
After choosing standard plants randomly in each small quadrat and removing the surface litter around plant roots, the rhizosphere soil in depths of 0-20 cm was collected into sterile plastic bags, frozen and quickly transported back to the laboratory for analysis. The soil samples were divided into two parts, one was sieved through a mesh of \ 2 mm in diameter, and preserved at 4°C for analysis of microbial biomass and enzymatic activity; the other was naturally air-dried, and sieved through a mesh of \ 1 mm in diameter for analysis of physical-chemical properties. Microbial populations were conducted by dilution plate method (Shen et al. 1999). The medium for actinomycesis named improved Gao compound medium, including soluble starch 2g, KNO 3 0.1g, K 2 HPO 4 0.05g, MgSO 4 Á7H 2 O 0.05g, NaCl 0.05g, FeSO 4 Á7H 2 O 0.001g, agar 1.8g, deionized water l00 mL, pH 7.4-7.6; the medium for fungi is named Martin-Bangladesh red medium, including Rose Bengal agar 3.5g, deionized water l00mL; the medium for bacteria is named beef extract peptone agar medium including beef extract (0.3%), peptone (0.5%) and agar (1.5%) in water. Root morphology was measured by CI-600. Fine roots were cleared in 10% KOH, stained with 0.05% w/v trypan blue for 24 h, and then were cut into 1.5cm-long segments (Phillips and Hayman 1970). Mycorrhizal infection rate was measured by the glass slide method. Mycorrhizal infection rate(%) = formation of twigs of the number of root segments/number of the measured root segments 9 100.
Soil pH and electrical conductivity (Ec) (soil:deionized water 1:5) were determined with a pH meter and electrical conductivity meter, respectively. Soil acid phosphatase activity was measured by the improved method of Tabatabai and Brimner (Zhao and Jiang 1986). Available phosphorus by the NaHCO 3 extraction colorimetric method (Bao 1998).

Statistical analysis
All data were subjected to one-way analysis of variance using SPSS program. Means were compared by Duncan's test at the 5% level.

Plant growth and root morphology
The impact of different Glomus species on the plant growth of Amygdalus pedunculata Pall. under field conditions is shown in Fig. 1. Compared with the non-inoculated control treatment, the plant height of single inoculation of R.i, single inoculation of F.m, co-inoculation of F.m and R.i increased by 40%, 21%, 19% respectively, and the plant crown width increased by 26%, 10%, 11%. Analysis of variance showed that single inoculation of R.i and single inoculation of F.m significantly increased plant height compared to the control treatment. Single inoculation of R.i proved better than the single inoculation of F.m and the coinoculation of F.m and R.i, while there was no significant difference between single inoculation of F.m and the coinoculation of F.m and R.i. There were no difference in the crown width between inoculated and non-inoculated control treatment. The data indicated that AM fungi can enhance plant growth of Amygdalus pedunculata Pall., in favor of revegetation in the mining subsidence area for ecological recovery.
The impact of different Glomus species on the root growth of Amygdalus pedunculata Pall. is shown in Table 1. Compared with the non-inoculated control treatment, single inoculation of R.i and single inoculation of F.m increased the root projected area and the number of root tips significantly, and single inoculation of R.i increased the root surface area significantly, while there was no significant difference in the average root diameter and volume.

Mycorrhizal colonization rate
Mycorrhizal colonization rate of different treatments for Amygdalus pedunculata Pall. is shown as Fig. 2. The average percentage of root colonization of three treatments was 78%, higher than that of the non-inoculated control treatment (59%), indicating that all the three Glomus species can increase root colonization. Among the three inoculation treatments, the percentage of root colonization by the single inoculation of F.m was the highest (91%), and the combined inoculation of F.m and R.i was the second (75%), and the single inoculation of R.i was the lowest (67%).

Soil water content
Soil water content in different treatments was shown in Fig. 3. One year after inoculation, soil water content was not significantly affected. The soil water content of single inoculation of F.m, single inoculation of R.i, and the coinoculation of F.m and R.i was 6.64%, 5.97%, 5.38% respectively, higher than that of the non-inoculated control treatment (4.71%), indicating that three inoculation treatments all had certain effect on increasing soil moisture, probably due to the increasement of soil organic matter and soil water capacity. There was no significant difference between these treatments.

Soil pH and soil electrical conductivity
Soil pH in different treatments for Amygdalus pedunculata Pall. after inoculation 1 year is shown in Fig. 4. There were some differences for different Glomus species, that is the soil pH of single inoculation of R.i and the co-inoculation of F.m and R.i were both higher than that of the noninoculated control treatment, while the soil pH of single inoculation of F.m was lower than that of the non- inoculated control treatment; there was no significant difference between single inoculation of R.i and the coinoculation of F.m and R.i. The electrical conductivity for different treatments is shown in Fig. 4. We can see that all the three inoculation treatments were higher than the noninoculated control treatment, indicating that inoculating AM fungi can increase the soil electrical conductivity.

Soil available phosphorus and acid phosphatase
The effect of different treatments on phosphorus and acid phosphatase activity are shown in Fig. 5. Phosphorus content of all the three inoculation treatments were higher than that of the non-inoculated treatment, indicating that inoculating AM fungi can increase soil available phosphorus, which is vital to the growth and health of plants, and favors the vegetation reconstruction in mining subsidence area. Soil acid phosphatase plays an important role in the transformation and bioavailability of organic phosphorus into inorganic phosphorus. The activity of soil acid phosphatase for different treatment is shown in Fig. 5. The activities of phosphatase of all the three inoculation treatments showed higher than the non-inoculated treatment.AM fungi accelerated the activities of phosphatase in soil rhizosphere, which can promote the transformation of soil insoluble phosphorus into plant available phosphorus, increasing soil available phosphorus.

Soil microbe populations
The effect of different treatments on soil microbe populations was shown in Fig. 6. The quantity of bacteria and fungi of all the three inoculation treatments tended to be higher than that of the non-inoculated control treatment, indicating that AM fungi increased the quantity of bacteria and fungi, especially bacteria. The population of different  soil microbes tended to decrease in the following order: bacteria (about 80% of the total number of microbes) [ actinomycetes (about 10%-20% of the total number of microbes) [ fungi (about 0.2%-0.7% of the total number of microbes). The study area was located at southeast Mu Us desert with poor soil quality, the increase in soil bacteria and fungi can improve the soil microbial and nutritional environment, conducive to the plant growth and local ecology recovery.

Discussion
Mycorrhizas are symbiotic associations established between certain soil fungi and most vascular plants. They play a good role in optimizing plant fitness and soil quality (Barea et al. 2011). In this study, different kinds of fungi species could infect plant roots of Amygdalus pedunculata Pall., which indicate that good symbiosis between AM fungi and Amygdalus pedunculata Pall. has been formed. Mycorrhizal colonization rate of different treatments were higher than that of the non-inoculated control treatment, which showed artificial inoculation to be effective in increasing root mycorrhizal colonization. In addition, the infection rate of different treats were different, showing that host plant and fungi select each other and its colonization rate may be probably influenced by host plants and AM fungal isolates. Mycorrhizal symbiosis could improve the resilience of plant communities against environment stresses, including nutrient deficiency, drought and soil disturbance and promote the growth rate of the plant. After inoculation for 1 year, plant height of inoculation treats presented significantly higher than that of control treatment. At the same time, certain indicators of the roots of inoculated plant were higher than that of no-inoculated plant. Rapid growth of plant is beneficial to the local ecological restoration, especially for ecological fragile mining areas. These results are consistent with former research (Wang et al. 2014).
Water is a key factor of plant growth, especially for the arid mining area where water loss is serious. Mycorrhiza can enhance nutrient acquisition and water absorption of host plants by extending hyphae beyond the nutrient depletion zone of roots (Wu et al. 2015). Through the development of hyphae network from mycorrhizal roots into the surrounding soil, hyphae directly enmesh soil particles. Besides AMF could release glomalin-related soil protein (GRSP) into soils, which could improve soil structure and improve water retention of rhizosphere (Bedini et al. 2009;Matthiasc et al. 2010). In this study, rhizosphere soil moisture content increase than control, probably due to the increase of soil organic matter and soil water holding capacity. After mycorrhizal inoculation, plant rhizosphere environment has also changed soil pH value and conductivity changed after mycorrhizal inoculation for 1 year, which may be due to the secretion caused a certain effect on rhizosphere soil biochemical reaction after mycorrhizal inoculation. Meanwhile, the content of available P for different inoculation treatments showed higher than that of non-inoculated treatment, which may be the result of higher soil acid phosphatase activities, which could promote the transformation of soil insoluble phosphorus into plant available phosphorus.
Microorganisms in the soil are critical in decomposing organic residues and recycling soil nutrients, and is one of the indicators of soil quality evaluation. Soil microorganism quantity and enzyme activity changes because of mining activities (Quadros et al. 2016). Arbuscular mycorrhizal inoculation can significantly improve the survival environment of rhizosphere microbes, is conducive to the improvement of the quality of the soil and the growth of plants. Research conclusions of our study were consistent with Camilla (Camilla et al. 2003).

Conclusion
Coal mining subsidence causes numerous environmental problems which have negative effects on vegetation growth. In this study, we found that AMF inoculation could promote the shoot growth and the root development of Amygdalus pedunculata Pall. The AMF inoculated plant showed higher root mycorrhizal colonization, soil acid phosphatase and available phosphorus as well as soil electrical conductivity compared to control. Single inoculation of R.i had good performance in plant height, soil moisture, and soil microbe population quantity; while single inoculation of F.m had good performance in soil available phosphorus concentration and soil fungi quantity. Associated inoculation of F.m and R.i can increase the soil electric conductivity and enhance soil acid phosphatase activity. AMF inoculation can be effective method for further ecological restoration in fertile environment in coal mine subsided areas.