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Caloglossa species diversity (Delesseriaceae, Rhodophyta) based on morphology and DNA data with emphasis on southeastern Brazil

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Abstract

Species of the marine red algal genus Caloglossa are among taxonomically confuse taxa as a consequence of their widespread phenotypic plasticity. Therefore, in this study, we assessed the specific diversity of Caloglossa from southeastern Brazil by combining both morphological observations and molecular markers. We used cytochrome oxidase subunit I (COI-5P) and partial sequences of ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene to investigate 15 taxa of Caloglossa. The four species previously referred to Brazil, C. apomeiotica, C. confusa, C. leprieurii, and C. ogasawaraensis, were also confirmed in this study, and C. rotundata is reported for the first time in the Atlantic Ocean; tetrasporangia are described for the first time for the latter species. Caloglossa confusa has its geographical distribution extended to northeastern Brazil, and C. apomeiotica represents the first record for São Paulo State. Furthermore, the rbcL sequence data indicate that the molecular distinction within the same species ranges from 0 to 5.4%, whereas interspecific divergence ranged between 2.4 and 14.5%, and COI-5P interspecific divergence ranged from 2.5 to 3.3%. The low intraspecific divergence observed (0-0.01%) for COI-5P results from the few samples sequenced.

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Acknowledgements

This work was partially supported by research grants from ‘Fundação de Amparo à Pesquisa do Estado de São Paulo’ (FAPESP, Proc. 2012/18775-6) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. 484647/2012-1). The first author thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Proc. 23038.000077/2010-38) for the Master’s dissertation fellowship. MTF thanks CNPq for the Productivity Fellowship (Proc. 303915/2013-7).

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Authors and Affiliations

  1. Postgraduate Program in “Biodiversidade Vegetal e Meio Ambiente”, Instituto de Botânica, Av. Miguel Estéfano, 3687, 04301-902, São Paulo, Brazil

    Cecilia Hissami Kano

  2. Postgraduate Program in “Botany”, Departamento de Botânica, Instituto de BiociênciasUniversidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, Brazil

    Fernando Santos de Sena

  3. Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão 277, 05508-090, São Paulo, Brazil

    Fernando Santos de Sena & Valéria Cassano

  4. Núcleo de Pesquisa em Ficologia, Instituto de Botânica, Av. Miguel Estéfano, 3687, 04301-902, São Paulo, Brazil

    Cecilia Hissami Kano & Mutue Toyota Fujii

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  1. Cecilia Hissami Kano
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  2. Fernando Santos de Sena
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Correspondence to Mutue Toyota Fujii.

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Kano, C.H., de Sena, F.S., Cassano, V. et al. Caloglossa species diversity (Delesseriaceae, Rhodophyta) based on morphology and DNA data with emphasis on southeastern Brazil. Braz. J. Bot 40, 551–564 (2017). https://doi.org/10.1007/s40415-016-0360-1

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