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Catalytic DNA Origami-based Chiral Plasmonic Biosensor

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Chemical Research in Chinese Universities Aims and scope

Abstract

Plasmonic circular dichroism(CD) has been emerged as a promising signal for building biosensors due to its high sensitivity and specificity. In the past years, DNA nanotechnology enabled diverse chiral plasmonic devices, which can response biomolecules and then generate dynamic plasmonic CD signals at the visible range. Although some of them have been successfully employed as biosensors, the detection sensitivity is still relatively low. Herein we report a chiral plasmonic sensor with an improved detection sensitivity by integrating catalytic hairpin assembly circuits into DNA origami structures. We tested two kinds of tumor marker RNA sequences as detection targets and it turns out that the detection limit is below 10 pmol/L, improving one order of magnitude compared to previous work. The chiral plasmonic sensor with internal signal amplification circuits can stimulate a variety of smart nano-sensors for biological detection and offer a promising strategy for pathogenic RNA detection with plasmonic CD output.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China(No.21977112), the Natural Science Foundation of Jiangsu Province, China(No.BK20190227), and the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000).

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Authors and Affiliations

  1. School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei, 230026, China

    Zhenyu Liu, Jiahao Pan, Chao Zhou & Qiangbin Wang

  2. CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and i-Lab, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123, China

    Zhenyu Liu, Jinyi Dong, Jiahao Pan, Chao Zhou & Qiangbin Wang

  3. School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China

    Jinyi Dong

  4. Frontiers Science Center for Transformative Molecules, School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200024, China

    Chunhai Fan

Authors
  1. Zhenyu Liu
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  2. Jinyi Dong
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  3. Jiahao Pan
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  4. Chao Zhou
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  5. Chunhai Fan
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  6. Qiangbin Wang
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Correspondence to Chao Zhou, Chunhai Fan or Qiangbin Wang.

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Liu, Z., Dong, J., Pan, J. et al. Catalytic DNA Origami-based Chiral Plasmonic Biosensor. Chem. Res. Chin. Univ. 37, 914–918 (2021). https://doi.org/10.1007/s40242-021-1115-x

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  • DOI: https://doi.org/10.1007/s40242-021-1115-x

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