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Electrochemical immunosensor for the detection of anti-thyroid peroxidase antibody by gold nanoparticles and ionic liquid-modified carbon paste electrode

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Abstract

In this work, a sandwich-type electrochemical immunosensor was designed based on the modification of the carbon paste electrode consisting of ionic liquid and graphite for the effective detection of anti-thyroid peroxidase antibody (anti-TPO). The ionic liquid carbon paste electrode surface was electrodeposited by biocompatible and electrically conductive gold nanoparticles (NPs) as a sensing platform. The human recombinant TPO (Ab1), anti-TPO antibody (Ag) and horseradish peroxidase (HRP)-anchored anti-TPO secondary antibody (HRP-Ab2) were successively immobilized on the Au NPs/CILE surface to construct the immunosensor. The immunosensor response signal was recorded by differential pulse voltammetry based on HRP catalysis in the reaction of H2O2 with O-aminophenol. An elevation in anti-TPO antibody concentration increased the current responses, probably due to the immobilization of a high amount of HRP-Ab2 on the electrodes for higher anti-TPO antibody concentration. The immunosensor, under the optimized circumstances, presented a low limit of detection (LOD) (6.0 ng mL−1) towards the anti-TPO antibody determination with a broad linear range (0.02–60.0 μg mL−1). Moreover, the prepared immunosensor showed good reproducibility and good stability.

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Acknowledgements

This research was financially supported by the Iranian National Science Foundation (INSF) (Grant No. 95012096) and Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.

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Authors and Affiliations

  1. Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

    Somayeh Tajik & Hadi Beitollahi

  2. Biotechnology Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

    Masoud Torkzadeh-Mahani

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  1. Somayeh Tajik
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  2. Hadi Beitollahi
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Correspondence to Hadi Beitollahi.

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Tajik, S., Beitollahi, H. & Torkzadeh-Mahani, M. Electrochemical immunosensor for the detection of anti-thyroid peroxidase antibody by gold nanoparticles and ionic liquid-modified carbon paste electrode. J Nanostruct Chem 12, 581–588 (2022). https://doi.org/10.1007/s40097-022-00496-z

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