Enhanced bio-composting of rice straw using agricultural residues: an alternate to burning

Rice straw which predominantly contains cellulose, hemicellulose and lignin, can be converted into value-added product such as bio-compost. The present study was planned to carry out rice straw degradation, added with agricultural residues like rice bran and fruit waste in different combinations, with standard fungal culture of Trichodermaharzianum MTCC 8230. Rice straw added with fruit waste and rice bran was moistened with sterilized water in five different proportions. The experimental trays were inoculated with spore suspension (1 × 108 spores/ml) of T. harzanium. The trays were observed for change in pH, appearance and chemical fibres (acid detergent fibre, neutral detergent fibre, and acid detergent lignin). A continuous increase in bulk density accompanied with a decrease in volume was observed in all the experimental trays including the control. There was an initial increase in pH from 7 to 9 which stabilized to 8 after 12 days of incubation. The crystalline structure of rice straw was transformed into amorphous one in a time frame of 28 days, with a significant decrease in lignin from 20–25% to 13–15% and an increase in crude protein from 5–17%. The crystalline rice straw was reduced to a brown crumpled and compact value-added composted biofertlizer with a high carbon and crude protein content.


Introduction
Amongst the different lignocellulosic crop residues in India, rice straw constitutes the largest proportion, with a production of 112 million metric tons per annum (Sukumaran et al. 2010). However, a vast proportion of this rice straw is subjected to open-field burning which is hazardous for the environment. Rice straw possess various several characteristics that make it an ideal feedstock for value-added products such as animal feed, bioethanol, lignocellulose enzyme, and bio-compost. The rice straw is chiefly composed of cellulose (32-47%), hemicellulose (19-27%) and lignin (5-24%) (Garrote et al. 2002). Therefore, the hidden value of rice straw can be explored by biological conversion of polysaccharides into aforementioned value-added products.
Composting is a bio-oxidative process which involves the conversion of organic waste to a stabilized final product free of phytotoxicity and pathogens, by the process of mineralization and partial humification (Inckel et al. 1996). It can be made on the farm at very low cost and can be used as organic fertilizer (Zucconi et al. 1981). Therefore, the rationale of the present study was to convert rice straw into organic fertilizer to mitigate its open-field burning.

Collection of rice straw and fungal culture
Rice straw was collected from School of Renewable Energy Engineering, Punjab Agricultural University (PAU), Ludhiana. The rice straw was washed thoroughly with tap water, chopped and sieved to 30 mesh size. Rice bran and fruit waste were collected from the local market. The materials 1 3 were stored in clean plastic bags at room temperature for further analysis and treatment. The fungal culture Trichodermaharzianum MTCC 8230 was procured from department of Microbiology, PAU, Ludhiana.

Experimental design
Ten clean and dried plastic trays were taken for the experimentation and 50 g (each) of rice straw was added in them. Fruit waste and rice bran were supplemented along with sterilized water in five different proportions ( Table 1). The contents were mixed thoroughly and inoculated with spore suspension (1 × 10 8 spores/ml) of Trichodermaharzianum @ 10% (v/v). The trays were covered with muslin cloth and incubated at 30 °C in a BOD incubator. The trays were observed every 48 h for change in physical appearance and sprinkled with water to prevent surface drying. The contents were weighed and their volumes were noted in a 1.0 L capacity measuring cylinder after every 4 th day till their bulk densities became constant. The data were used to calculate bulk density by the following method: The pHs of the contents were also recorded by using a pH paper strip of range 3-10. The contents were replenished in trays that were placed in BOD incubator maintained at 30 °C.

Chemical fibre analysis
To evaluate the biodegradation rate, the samples were analyzed for acid detergent fibre (ADF), neutral detergent fibre (NDF) and acid detergent lignin (ADL) by the method of Goering and Van Soest (1970). The crude fibre protein determination was carried out with the help of department of Bulk density g∕cm 3 = Weigh of contents (g) Volume cm 3 .

Statistical analysis
All experiments were carried out in triplicates and all the values are mean ± standard error (SE), calculated using the MS excel program.

Changes in bulk density, pH, and physical parameters
The results presented in Fig. 1 revealed a continuous increase in bulk density in all the trays including the control, though there was more increase in the first 12 days. The increase in bulk density represented the compactness of rice straw which is due to decrease in volume. The volume decrease was also accompanied with decrease in weight due to reduction in moisture level and volatile solids. The reduced weight and stability of compost facilitates its storage and transportation to faraway nursery and residential value-added markets (NRAES 2001). The pH of the compost showed an initial increase from 7 to 9 which stabilized to 8 after 12 days of incubation. A previous study on composting of cow manure and rice straw with cow urine addition (pile A) and cow manure and rice straw without cow urine addition (pile B) reported a rise in pH with start of composting and a final pH of 7.8 for pile A and 6.7 for pile B (Phong and Quynh 2018). Dewes (1996) reported pH of 7.0-8.5 to be optimum range for composting that could reduce volatilization speed of ammonia and the nitrogen losses. Smars (2002) found that composting is inhibited at pH below 6. Sundberg and Jonsson (2008) reported faster decomposition in biowaste and more stable compost product at higher pH condition. The morphological appearance of the compositing rice straw depicted change in color, structure, and odor. The color changes of the compost help in evaluation of compost maturity in the field in a swift and convenient manner. As observed in the present study, the rice straw compost turned dark brown from an initial yellow color and on 15th day, a profuse growth of fungal mycelium became apparent. A change in texture to crumpled and crusty was observed from an initial smooth texture (Table 2, Fig. 2). The degrading material had putrid odor during 12-15 days of incubation and was pleasant earthy at the end of the compositing process (28 days). A previous study also recorded similar physical characteristic features of rice straw and sewage sludge compost. The color of the blend at the start of the composting process was observed to be yellowish grey while that at the end was

Changes in chemical characteristics of compost.
The recalcitrant nature of rice straw is due to the presence of crystalline cellulose with a high degree of polymerization and branched low-molecular weight hemicelluloses, which are laminated partly, by a lignin matrix. The lignin component of the rice straw is decomposed into fulvic acid and humic acids by lignocellulolytic fungi, by humification process, which finally makes the substantial proportion of soil organic matter (Huang et al. 2008). These humic substances serve to provide energy for microbial growth and help in the regulation of carbon cycle (Veeken et al. 2000). The compost material in the present study depicted change in composition of rice straw during 28 days of experiment. The crystallinity of rice straw was transformed into amorphous with a significant decrease in lignin from 20-25% to 13-15%, thus making cellulose and hemicelluloses available for microbial degradation. The maximum reduction in lignin was observed in treatment number 5 that had high fruit waste addition and moisture level (Table 3, Fig. 3). The crude protein also increased to 17% in the compost material which was as low as 5% at the start of the experiment (Table 3).

Conclusion
The crystalline rice straw with a low bulk density, which occupied a large volume was reduced to a brown, crumpled and compact value-added composted biofertilizer. The said biofertilizer possessed a high carbon and crude protein content that can help provide a green technique for the improvement of soil health by supplementing the C and N pool of the soil and thus alleviate the farming cost.