Design of biogas digester with thermophilic pretreatment for reducing fruits wastes

This study aimed to design a biogas digester that works thermophilically and mesophilically and tested its performance to produce biogas and digestate. This study used some experimental methods, which consists of: (1) design and construction process of the digester which can facilitate the thermophilic process as a pretreatment of the feedstock and the anaerobic digestion process of the substrate; (2) determination of the quantity of biogas, liquid digestate, and compost; and (3) testing digestate quality as a liquid organic fertilizer for Ipomoea reptans. The built biogas digester was able to accommodate thermophilic digestion that runs intensely in the pretreatment tank, where the complex organic compounds, namely cellulose and hemicellulose, decomposed intensively, so that it becomes a suitable substrate. As raw material, every 4 kg of banana waste, can produce biogas, digestate and dry compost of 10,200 cm3 (highest yield), 5900 mL, and 1420 g, respectively. The highest Ipomoea reptans growth was achieved by digestate treatment from banana waste. Thermophilic pretreatment could shorten the processing time to 3 days. Digestate treatment as a liquid organic fertilizer is able to provide a better supply of nutrients for plants.


Introduction
The handling of waste must begin to be realized as part of all citizens' responsibility, so that the conventional handling waste "throw-take-carry-pile" is not justified. This method often leads to accumulation problems if the number of employee and fleets was inadequate, so this method has to be improved and even replaced (Indonesian Government Regulation Number 81 of 2012). Biogas technology is a waste processing technology that has several advantages, such as it can be applied in each location and without forming a by-product. This technology can prevent environmental problems that commonly occur in open dumping and centralized processing. A broad landfill area can be used for more productive activities. This process will lead to sustainable and balanced production of organic fertilizer for soil applications, which will increase yields and improve soil health and environmental quality. The method can be a model of waste processing that can be replicated, especially for developing countries (Sudharmaidevi et al. 2017).
Organic waste is a valuable resource for fertilizer, compost, and fuel. Organic waste is a valuable source of fertilizer, compost and fuel. This biogas production with anaerobic digestion also leaves organic residues in the form of digestate and compost that are rich in nutrients. The use of biogas fuel compared to fossil fuels will reduce the carbon footprint. If these digestates are applied in crop production, the available nutrients which already exist in the nutrient cycle will be recycled and the sustainability of the biogas production process will increase. The use of organic fertilizer also contributes to maintaining and/or improving soil quality (Sogn et al. 2018).
The food waste is assumed to be shredded, sieved and then sent to a digestion tank. The output of this biological process is two valuable products: biogas which may be used 1 3 for either heat or power generation; and digestate that may be utilized as a soil fertiliser (Salemdeeb et al. 2018).
The waste management in Indonesia has been changed by reducing the source and recycling approach of materials, namely 3R (reuse, recycle, reduction). Therefore, all citizens are expected to change the way they view and treat waste as an alternative resource, which can be used optimally or over-used and recycled (Indonesian National Legislation Number 18 of 2008. Furthermore, Indonesian Government Regulation Number 81 of 2012 provides that waste management consists of five stages: segregation, collection, transportation, processing and final processing of waste by all citizens. The rules are applied gradually, planned, and based on clear policies and strategies. Temperature is one of the most important factors in the succession of composting micro-organisms, and it is relatively easy to ascertain the microbial succession from mesophiles to thermophiles, or conversely from thermophiles to mesophiles, using different incubation temperatures with, for example, the dilution plating method (Nakasaki et al. 2005).
The advantage of using the thermophilic enzymes for conducting biotechnological processes at elevated temperatures are: reducing the risk of contamination by mesophilic microorganisms; decreasing the viscosity of the reaction medium; increasing the bioavailability and solubility of organic compounds; increasing the diffusion coefficient of substrates and products resulting in higher reaction rates (Gomes et al. 2016).
The creative idea of the research offered was to construct a digester that could guarantee the bacteria consortium to effectively work on thermophilic process of 55-60 °C as a pretreatment action against complex organic matter, such as in fruit wastes, so it became a ready feedstock for the anaerobic digestion process, which generates biogas and digestate.

Digester design
Design a digester that can guarantee: (1) a consortium of microbes working thermophilic (55-60 °C) in the predigester tank; and (2) the microbial consortium works with anaerobic digestion (methanogenesis) in the main digester tank.
The understanding of the design process is important both to manage the design activity and to aid the improvement of products and the overall efficiency of engineering-based companies; it is also the foundation on which a lot of design research is based. It is suggested that understanding this process relative to the creative process will give insight into where and when resources should be focused to enhance creative performance and also the resulting 'quality' of the product designed (Howard et al. 2008). The designers generally worked in sequence according to these stages, but it was not impossible that they returned to a previous step if there were mistakes (Rahmat et al. 2013). The stages of the study are shown in Fig. 1.
The digester testing consisted of two experimental stages, namely: (1) determining the digester capacity that included production of biogas, digestate, and compost; and (2) determining the digestate quality as an organic fertilizer.

Materials
Preparation of fruit waste. Banana and papaya wastes were collected from five different waste ponds (10 kg each) as samples representing the Cilembang vegetable market, Tasikmalaya, Indonesia. The waste is mixed homogeneously and then crushed with an electric blender. The crushed wastes were converted into slurry by mixing hot water in weight ratio of 1:1. Furthermore, the mixture was made as the object of thermophilic pretreatment.
Preparation of thermophilic bacteria. Consortium of thermophilic bacteria was obtained from organic matter that decomposed in hot springs at Galunggung Volcano in Tasikmalaya District. The inoculants of thermophilic bacteria were cultured in the laboratory at temperatures of 55-60 °C for 30 days.

Arrangement of treatment
The aim of the experiment was to test the effect of four waste treatments biogas production and digestate performance. The treatment of fruit waste was varied as A (banana waste), B (papaya waste), C (banana waste + papaya waste in ratio of 1.5:1), and D (banana waste + papaya waste in ratio of 1:1), respectively.
The experimental design was used in a randomized block design and five treatments were carried out. Data analysis was done by analysis of variance (ANOVA) and Duncan's test (Gomez and Gomez 1984).

Materials
Preparation of Ipomoea reptans nursery. Each Ipomoea reptans planting unit consists of 20 plants on soil-growing media in plastic tray size of 30 cm × 30 cm × 5 cm. The experiment had 5 treatments and 5 repetitions, so it took 25 planting units.
Arrangement of treatment. The experiment aimed to test the effectiveness of four digestate as liquid organic fertilizer on the Ipomoea reptans growth. The treatment of digestate was varied as A′ (digestate from treatment A), B′ (digestate from treatment B), C′ (digestate from treatment C), D′ (digestate from treatment D), and E (water without digestate as comparator), respectively.

Experimental set up
The experimental design was used in a randomized block design and five treatments were carried out. Data analysis was done by analysis of variance (ANOVA) and Duncan's test (Gomez and Gomez 1984).
Variables of response as growth parameters were measured on indicator of (1) length of shoot; and (2) weight of plant biomass. Measurement was taken on a 21-day-old plant.
The performance of a biogas digester is determined by many factors, among which are microbial population, acidity (pH), carbon-nitrogen mass ratio (C/N ratio), operating temperature, substrate particle size, organic loading rate, hydraulic retention time, total solids content, and reactor configuration, i.e., batch or continuous, single stage or two stages (Haryanto et al. 2018). Those things explained that variations in design, dimensions, and operational parameters of a digester will effect on its performance.
The effect of stirring, operation temperature, organic loading rate and reactor design on the methane production rate and methane yield was studied. For the same duration of digestion, the unmixed digester produced 20-50% more methane than mixed system. Two-stage design which separated the soluble components from the solids and treated them separately had more rapid kinetics than one-stage system, producing the target methane potential in one-half the retention time than the one-stage system. The two-stage system degraded 6% more solids than the single-stage system (Dhoble and Pullammanappalli 2014). The study was intended to understand the biogas production process at mesophilic temperature (37 °C). The organic waste from kitchen was used to make up substrate, which was anaerobically digested in an experimental continues stirred tank reactor (CSTR). Inhibition phase was detected when the graph was plotted for first 60 days and about 28th day, it was inhibited. Simple regression yielded a good predictive model that gave a correlation of 0.995, despite including the inhibition phase in complete analysis. Such models can also be used to keep check on digestion process for optimization of biogas yield and deciding the substrate feeding rate and concentration on time scale (Ali et al. 2016).
Biogas produced from cow manure in a 25-L semicontinuous digester and working at ambient temperatures turned out that the loading rate affects the yield and productivity of biogas, where the increase in substrate loading rate decreased the production and yield of biogas (Haryanto et al. 2018).
The batch experiment was carried out in a 250-mL Duran bottle as a bioreactor with a working volume of 200 mL placed in a water bath shaker (100 revolutions per min) under the thermophilic temperature (55 ± 2 °C). The twostage thermophilic digestion process could effectively enhance biohydrogen and biomethane production, as well as reducing organic waste simultaneously (Wongthanate and Mongkarothai 2018). As found previously, thermophilic bacteria are of great importance in organic matter decomposition during thermophilic composting. No remarkable changes were observed among colonies on agar plates over the first 3 days of the process, suggesting that the thermophilic microflora stabilized soon with no significant succession within this time. Mesophilic fungi were detected only in the initial stages of composting, before quickly diminishing. Thermophilic fungi were not detected at time during the experiments that composting temperatures of 60 °C or higher are incompatible with the growth of even thermophilic eumycetes (Nakasaki et al. 2005).

Biogas production
The volume of daily biogas yield from the four treatments is shown in Fig. 3. The feedstock of all treatments contain easily hydrolyzed organic compounds such as sugar, fatty acids, and amino acids, which can be quickly converted into biogas and other gases, and peak after two digestive days. Observed after 4 days of digestion, the daily volumes of biogas yield were close to zero, because feedstock was almost completely digested. This result contrasted with previous studies, which concluded that the biogas yield on the 10th day was 7.23 L in the 36-L digester which operated at a daily temperature of 28.7-29.1 °C. The best biogas productivity was 77.32 L/kg VS found at an organic loading rate of 1.31 g/L/day (Haryanto et al. 2018). The above research proved that the thermophilic pretreatment process in fruit waste could provide more feedstock for the next process of anaerobic digestion (methanogenesis), so the process runs faster and more biogas and digestate were produced.
The highest total biogas production results in treatment C, followed by treatments A, B and D (Table 1). Treatment C has a C/N ratio which is more suitable as a substrate for anaerobic digestion process. Ward et al. (2008) stated that a very strong reason for anaerobic feedstock digestion is adjustment of carbon-nitrogen ratio (C/N). Microorganisms generally use carbon and nitrogen in a ratio of 25-30: 1, but C/N ratio is often much lower than this ideal. Subsequent study show that there was an interaction effect between temperature and C/N ratio on the performance of anareobic digestion. When the temperature is increased, it is necessary to increase the C/N ratio of the substrate, so that the risk of ammonia inhibition is reduced. Increased C/N ratio reduces the negative effects of ammonia and maximum methane yield potential is achieved at C/N 25 and 30 ratios at 35 ° C and 55 °C, respectively (Wang et al. 2014). The efficiency of anaerobic digestion of food waste increased from 69 to 74%, when the C/N 1 3 ratio was 17-26, respectively. The results generally show that increasing the C/N ratio of food waste results in better pH stability and increases methanogenic activity.
Previous research has shown that the generation of thermophilic biohydrogen from food waste was achieved at optimal conditions at initial pH 7.0 and C/N 30 ratio. Hydrogen-fermented waste can be used as a substrate for the production of thermophilic biomethane, which was obtained optimally at an initial pH of 7.0 and a C/N ratio of 30. In this optimal condition, COD removal efficiency can be achieved in the range of 70-90%. Therefore, a two-stage process in a controlled system to process organic food waste material at optimal conditions can increase bioenergy production (Wongthanate and Mongkarothai 2018).
The total of biogas production per 8 L of processed substrate for the 7 days retention time in series was: B (6050 cm 3 ), D (6950 cm 3 ), A (10,200 cm 3 ), and C (12,750 cm 3 ). The highest production in C treatment was due to the substrate condition with the pH and C/N ratio approaching the optimal value. The optimum conditions for acidogenesis and methanogenesis at pH range from 6.4 to 7.2, while for the anaerobic digestion process in C/N range from 20 to 30 (Wang et al. 2014).
Pre-treatment (PT) of active waste sludge (WAS) was optimized by thermochemical treatment at 70 °C and 0.04 M NaOH solution for 1 h. The maximum PT efficiency in sludge disintegration can be achieved in combination with heat treatment and low chemical-related costs. Some advantages of the PT were increasing the yield of methane and biogas, causing increased degradation of sludge during anaerobic digestion, the drainage capacity of higher digestible sludge, and reducing the amount of waste to be disposed of in relation to WAS from waste water treatment plants (Nagler et al. 2018).
Referring to the daily and total biogas production, it is proven that single or mixed fruit waste can produce biogas. The biogas production through anaerobic digestion offers significant advantages over other forms of bioenergy production. The digestate allows an accurate dosage and integration in a fertilization plan with a reduced application of additional mineral nitrogen fertilizers (Weiland 2010).

Digestate yield
As shown in Table 2, papaya waste produced the highest volume of digestate and was followed by decreasing by mixture of banana waste-papaya waste in weight ratio of 1:1, banana waste-papaya waste in weight ratio of 1.5:1, and banana waste. The difference in the quantity of digestate results was due to the water content of the waste papaya higher than the others. In contrast, the highest weight of dry compost was produced by banana waste and followed by a decreasing by mixture of banana waste-papaya waste (1.5:1), papaya waste, and mixture of banana waste-papaya waste (1:1). This condition can be explained that the weight  1 3 of dry compost, as plant solid-biomass was constitutive to the quantity of digestate.
Digestate quality testing Table 3 shows that all treatments provide different levels of ground spinach growth than controls. The highest growth was achieved by the treatment of liquid organic fertilizers derived from banana digestate liquid (LD) followed by decrease by digestate from a mixture of banana-papaya (1.5:1), mixture of banana-papaya (1:1), and papaya digestate. The experiment proved that LD's treatment as a liquid organic fertilizer was able to be an adequate supplier of nutrients for ground spinach plans. Digestates are content pH, NH 3 /NH 4 , and metal elements, mainly Cu, Zn and Mn higher than livestock manures. Digestates may have an N, P and K macronutrient composition that was not optimal for plant growth needs, but variations in P and K content were within the suitable range for crop production (Sogn et al. 2018). The anaerobic digestion process results in a mineralization of organically bounded nutrients, in particular nitrogen and in a lowering of the C/N ratio. Both effects increase the short-term N fertilization effect. Due to the improved flow properties, the digestate can penetrate faster in the soil which reduces the risk for nitrogen losses by ammonia emissions. Anaerobic digestion results also in a significant reduction of odors and in a positive change in the composition of odors. Measurements have shown that up to 80% of the odors in the feedstock can be reduced (Weiland 2010).
Sediment from biogas digester can be used as material for the generation of vermicompos. The vermicomposts are rich in N-P-K nutrients and the C/N ratio is below 15 which shows the agronomic role as a soil conditioner and manure. The quality of raw materials determines the physico-chemical characteristics of vermicompost. The results show that vermicompost can be introduced as a technology to convert organic waste into value-added products (Yadav and Garg 2016).

Conclusions
The biogas digester was performing of thermophilic initial decomposition in its pre-treatment tank. In the pretreatment tank, complex organic compounds, cellulose and hemicellulose were decomposed intensively converted as substrates for anaerobic digestion.
The thermophilic process involved as a pretreatment can shorten digestion time to 3 days. As feedstock, each 4-kg banana waste, papaya waste, mix of banana-papaya waste in weight ratio of 1:1, and mix of banana-papaya waste in weight ratio of 1.5:1 produced biogas of 10,200 cm 3 , 6050 cm 3 , 12,750 cm 3 , and 6950 cm 3 , respectively. Furthermore, digestate of 5900 mL, 6800 mL, 6300 mL, and 6500 mL was produced; also dry compost of 1420 g, 1130 g, 1337 g and 1098.58 g was produced, respectively.