Manurial value of khat waste vermicompost from Awday, Harar town, Ethiopia

Catha edulis, commonly called as khat or chat is an evergreen plant cultivated in Ethiopia for its stimulant leaves. The young leaves are chewed as a part of social recreation and the older leaves along with the twigs are discarded. This forms the major part of municipal solid waste that is dumped in large quantity in Harar region. The present study was carried in Haramaya University, Ethiopia to decompose the khat wastes along with crop residues and different animal manures (cow dung, goat manure, poultry manure and swine manure) using the earthworm Eisenia foetida. The collected khat wastes along with crop residues and different animal manures were vermicomposted and assessed for nutrient contents using standard procedures. The result of the experiment indicated that all the parameters were improved by the decomposition using earthworms and the C/N ratio was within the limits that indicate the maturity of the compost. The organic carbon, organic matter, total nitrogen, phosphorus, copper, iron, zinc and manganese contents were analyzed and found to be improved after decomposition. The added crop residues and animal manures enhanced the decomposition process and manurial value of the khat leaf waste. The nutrient content of the vermicomposts revealed khat to be a suitable substrate for vermicomposting thereby making efficient utilization of solid wastes. This can be an effective technology to reduce the solid waste and the resultant product can be used for improving soil fertility and crop production by the farmers.


Introduction
In Ethiopia, Horn of Africa, there are different organic wastes being produced annually by different activities like agriculture, agro industries, industries and municipal solid waste. Agricultural wastes are most preferably used as fodder or biofuel. The solid wastes are being dumped or burnt without proper recycling. The capital city of Ethiopia, Addis Ababa produces 0.5 kg per capita per day solid waste and the management of these wastes is difficult (Desta et al. 2014). It includes organic and inorganic wastes. There are different management problems encountered by the public and the municipal authorities (Regassa et al. 2011). Next to this, Bahir Dar, another major city of Ethiopia, also faces difficulty in handling of wastes generated daily (Lohri et al. 2014). The city of Debre Birhan produces much of organic waste that mainly includes food and kitchen wastes in urban solid waste and the recycling possibilities are yet to be exploited (Tyagi et al. 2014). Similarly, Debre Markos also faces the difficulty of management of solid wastes produced by the city (Tiwari and Tiwari 2012). Jimma city of Ethiopia produces a huge amount of waste that needs to be recycled (Getahun et al. 2012). Dessalegn et al. (2012) have suggested that the source separated municipal solid waste can be composted and used as manure. Shambu town of Ethiopia produces a large quantity of solid waste which needs to be managed effectively (Aboma and Reddy 2013). Dire Dawa city also produces a large quantity of municipal solid waste that could be converted into manure (Alemayehu 2013).
Khat is the export crop after coffee and oil seeds and plays a considerable role in the national economy. Its trade has a great influence on the farmers who cultivate them, the traders and exporters, and also have a high impact on the social, economic and health status of the society (Aden et al. 2006;Bongard et al. 2011). Catha edulis Forsk is an evergreen tree of Celastraceae family cultivated for its stimulant leaves primarily in East Africa. Presently Ethiopia, is the main producer and exporter of khat (Andualem 2002;Bongard et al. 2011;Ezekiel 2005;Yeshigeta and Abraham 2004;Wabel 2011). The culture of chewing khat has increased the demand for leaves but results in consequences of health and socio economic conditions (Dawit et al. 2005;Kandari et al. 2014). The leaves are chewed by a large population in Ethiopia (Dawit et al. 2005). The leaves are now common among students in different khat using countries in addition to motor vehicle and truck drivers and other working community (Yeshigeta and Abraham 2004;Reda et al. 2012;Alsanosy et al. 2013;Megerssa et al. 2014). The negative social and physical effects need to be considered while substituting the coffee cultivation by khat (Dechassa 2001). The Harar town of Ethiopia also produces large quantity of municipal solid waste. Major portion of it is khat waste discarded by users and exporters. The waste production is also enormous that needs proper disposal mechanism. Figure 1 shows the cultivation of khat in different parts of Ethiopia.
The khat is now cultivated in almost one-third area of coffee cultivation (Telake 2007). The transport facilities have expanded the khat utilization across the country (Gesese 2013;Andualem 2002;Guesh 2012). Khat is replacing coffee (Dube et al. 2014) and cereals (Taye and Jens 2003) in different parts of Ethiopia due to its economic benefits. It is bringing economy to the country, giving livelihood to a number of people by involving in different activities starting from agriculture to the export. The young leaves are chewed and the older leaves along with the twigs are discarded that forms the major part of municipal solid waste in Harar region which is a main place of khat cultivation, use and export. The town of Awadey known for its export of khat here adds a large quantity of solid waste as the older leaves are discarded along with twigs by export packagers and chewers of khat that are accumulated everyday in the town. Argaw et al. (2015) have shown that the khat waste could be a promising substrate for mushroom cultivation. It promises the utility of decomposition of the waste and converting it into manure. Different cities of Ethiopia producing bulk amount of municipal solid waste (organic waste) can be converted into organic manure by vermicomposting technology.
Vermicomposting is a bioconversion process where earthworms feed on the organic waste to produce more earthworms, vermicomposts and vermiwash as products. The vermicomposting technology is fast growing with its pollution-free, cost-effective and efficient nature to convert the rural and urban wastes into organic manure. Hemalatha (2012) has reported that the vermicomposts prepared from the partially decomposed fruit waste with paper and tannery industry sludge using Eudrillus eugeniae was found to be an effective method. The waste materials are being Fig. 1 Commercially significant khat-growing regions in Ethiopia. The map is produced by superimposing the growing regions identified during field visits on existing roadmaps of the country. Source Gessesse (2013) produced enormously that need to be managed without any adverse environmental impacts. There are many implications due to the accumulation of wastes in the soil. It gives immense odor due to the production of different gases, and pollutes the natural sources of water and also soil gets contaminated with pathogens. To overcome these difficulties, vermicomposting can be considered as an option that can reduce the pollution due to waste and produce valuable manure that can be used by farmers for improving soil fertility and crop production in a sustainable way. Gezahegn et al. (2012) have vermicomposted coffee husk, enset waste, khat waste and vegetable waste using the epigeic earthworm Eisenia foetida and found to be as a good option for improving solid waste management in Ethiopia and production of excellent biofertilisers for agronomic purposes. The analysis of vermicomposts revealed that there is an increase in nutrient content and reduced C/N ratio. Thus, the vermicomposting plays a significant role in protecting environment as it uses the waste as raw material and building up the soil fertility and improving the soil health for sustainable agriculture.
Different species of earthworms are used for composting of animal, plant, pharmaceutical, food waste and sewage waste over vermicomposting periods ranging from 28 to 120 days using these worms. The predecomposition process of substrates for vermicomposting ranges with a varying temperature from 18 to 67°C, pH 5.9-8.3 and moisture content up to 80 %. The vermicomposts and vermiwash produced are rich in nitrogen, phosphorus and potassium and can be applied to plants as biofertilisers (Manyuchi and Phiri 2013). Pigatin et al. (2016) have documented that co-vermicomposting of filter cake and orange peel with cattle manure is effective to reduce the wastes and utilize it for application in sustainable agriculture. Parthasarathi et al. (2016) have reported vermitechnology as an effective method of producing bioorganic fertilizer from cashew leaf litter and animal dungs. The present work was carried out to prepare vermicompost using khat waste from Awday town of Harar enriched by different combinations of farm wastes and organic manures.

Description of the composting site
Research field of RARE of Haramaya University campus is located in East Hararghe zone of western Oromiya National Regional State. The experimental field is located at 9°26 0 N latitude and 42°3 0 E longitude. The average altitude is about 1980 m asl. It is located in the semi-arid tropical belt of eastern Ethiopia and is characterized by a sub-humid type of climate with mean annual rainfall of 780 mm. The site receives bimodal rainfall (March-April 25 % rain and June-September 45 %). The mean monthly temperature ranges from 9.2 to 23.2°C.

Raw materials and composting process
Catha edulis leaf wastes were collected from Awday town and cut into small pieces. The farm wastes were collected from fields of Haramaya University and they too were cut into smaller pieces of 2.5-5.0 cm. The animal manures were obtained from agricultural farm of Haramaya University. The substrates in the ratio of 10:1:1 (weight in kg) were used for the vermicomposting process as indicated below.

Substrates and combinations used in vermicomposting
Vermicomposting process Cement tanks measuring 1 m 9 1 m 9 1 m were used for the vermicomposting. The substrates were mixed in 10:1:1 ratio. The mixtures were left with moisture for 30 days to degrade into smaller particles to make it palatable for the worms. The moisture and temperature were maintained by sprinkling water and turning the mixture. Composting earthworm species E. foetida commonly known as red worm were introduced after 30 days. The moisture content was maintained to 60-70 % and the tanks were covered with moist jute bags to protect the worms from predators and prevent moisture loss. After 60 days of vermicomposting the samples were collected and analyzed for quality.

Assessment of quality of the vermicomposts
The collected samples were shade dried and processed as required for different physicochemical analyses in Soil Chemistry laboratory of Haramaya University, Dire Dawa, Ethiopia. Total organic carbon content in the samples was measured by Walkley and Black (1936) method and the organic matter was calculated by multiplying the percent organic carbon by 1.724 assuming that organic matter is composed of 58 % carbon. The total nitrogen (TKN) was determined by micro-Kjeldahl method (Humphries 1956). The available phosphorus was estimated according to Jackson (1973). The micronutrients namely zinc, copper, iron and manganese were assessed using atomic absorption spectrophotometer (Jackson 1973) method. The C/N ratio was calculated by dividing the total nitrogen by the organic carbon content. The nutrient value of the khat waste materials is indicated in Tables 1 and 2. This gives an idea about the substrate material that was utilized in the present study.

Nutrient status of khat vermicomposts
The results obtained from the analysis of decomposed khat waste are recorded in Table 3. It exhibits the manurial value of the decomposed products in dry weight basis.
The contents of total organic carbon, organic matter, total nitrogen, available phosphorus, and micronutrients namely zinc, copper, iron and manganese varied among the treatments. The C/N ratio of mature compost was within the limits and the end product was with earthy aroma and dark color.
The values of organic carbon (2.56 and 2.55 %) and organic matter (4.33 and 4.38 %) obtained from the analyses were comparable in T 2 (C. edulis leaf wastes ? farm wastes ? goat manure) and T 4 (C. edulis leaf wastes ? farm wastes ? swine manure), respectively. This could be due to the mineralization of animal manure applied in the treatments that enhanced the decomposition process and worm activity. The total nitrogen was high in T 3 (1.56 %), poultry manure-added vermicomposts. This could be attributed to the high nitrogen mineralization of volatile nitrogen in poultry manure. Available phosphorus was comparable among the treatments with a value of 60.49 ppm in T 2 . A high value of copper (20.93 ppm), iron (443.33 ppm) and zinc (42.31 ppm) was also observed in T 3 (with poultry manure). Manganese content was high in T 4 (108.17 ppm). The organic carbon and organic matter were comparable in T 2 and T 4 whereas the nitrogen and micronutrients, copper, iron and zinc were high in T 3 , manganese was in T 4 . The C/N ratio of the prepared vermicomposts ranged from 2.21 in T 3 to 9.52 in T 4 . The values were found to be within the permissible limits for composted materials.

Discussion
Similar to the present study, the content of organic carbon (C) was 1.03 % and organic matter was found to be 1.78 % in Parthenium vermicomposted with cowdung using E. eugeniae earthworms (Hiranmai et al. 2012). Mekonnen and Argaw (2015) have also reported the beneficial effect of using khat waste after vermicomposting and applying for improving soil fertility and crop production. They decomposed khat waste with eucalyptus leaves and recorded extractable phosphorus (0.57 %) and total nitrogen (1.4 %) which is comparable to the present study. The values obtained for nitrogen were within the range of 0.51-1.61 % as referred from Nagavellamma et al. (2004). Vermicomposting of vegetable wastes with cowdung and earthworm Eudrillus eugeniae were also found to improve  The values are means of triplicates the quality of end product in addition to reducing the waste quantity (Hiranmai and Vijayakumari 2002). The substrates used in vermicompost influence the stability of the final product of decomposition (Hu et al. 2008). It was observed in earlier studies by Vijayakumari and Hiranmai (2004) that there was a gradual reduction in organic C content from fresh poultry droppings (2.00 %) to composted (0.61 %) and vermicomposted (0.93 %) samples. The organic matter content was also lower in composted poultry droppings (1.05 %) compared to fresh poultry droppings (3.44 %) and vermicomposted poultry droppings (1.59 %). The decomposition of poultry droppings using E. eugeniae was found to be beneficial in improving the quality to be used as organic manure (Vijayakumari and Hiranmai 2005). Gezahegn et al. (2012) have vermicomposted coffee husk, enset waste, khat waste and vegetable waste using the epigeic earthworm E. foetida and found it as a good option for improving solid waste management in Ethiopia and production of excellent biofertilisers for agronomic purposes. Parthenium was vermicomposted with farm wastes and animal manures (cow dung, goat manure, poultry manure and swine manure) using E. foetida, the red worm. The pH, organic carbon, organic matter, total nitrogen, phosphorus, potassium, sodium, calcium, magnesium, copper, iron, zinc and manganese contents were analyzed and found that these nutrient contents were improved. The results revealed the economic feasibility of the vermicompost (the organic manure) production and certify the ecofriendly nature of its technology Hiranmai 2015).
The vermicompost prepared with cattle manure, food waste and paper was found to be rich in nutrients and vermicomposts contain plant growth-regulating materials like plant growth hormones and humic acids that can increase the germination, growth and yield of plants (Atiyeh et al. 2002;Arancon et al. 2006). Compost maturity is the level of decomposition that can be defined by the microbial biomass and decomposition of phytotoxic compounds in compost (Wu et al. 2000;Wu and Ma 2001). The co-composting by improved nutrient release reduces the cost and use of fertilizers. Hiranmai and Anteneh (2015) have recorded C/N ratios to be within limits for paper vermicompost prepared using E. eugeniae. Hait and Tare (2011) have also reported C/N ratios less than 20 for activated sludge vermicompost. This indicates organic matter stabilization and reflects a satisfactory degree of decomposition and maturity of organic wastes. The loss of organic carbon as CO 2 due to microbial respiration and addition of earthworm N excrement results in good C/N ratios. Hemalatha (2012) has also reported that the vermicompost prepared from the partially decomposed fruit waste with paper and tannery industry sludge using E. eugeniae was found to be an effective method. The analysis of vermicomposts revealed that there is an increase in nutrient content and reduced C/N ratio. Thus, the vermicomposting plays a significant role in protecting environment as it uses the waste as raw material and building up the soil fertility and improving the soil health for sustainable agriculture.
The substrate used in the present study was khat leaves, farm wastes and animal manures. This was to assess the efficiency of different manures and farm wastes in decomposition process. Ethiopia being agriculturebased country with farm and animals, the availability of wastes and manures varies in different regions. This can be used by the farmers for production of organic manures and used to maintain soil fertility and improve crop production. The country faces the soil fertility problems in different regions that can be managed by the use of available organic resources. In addition, the use of expensive chemical fertilizers that reduce the soil fertility can be minimized by developing the on-farm compost methods that help the farmers to use locally available resources.
As the obtained results show improvement in different parameters in different treatments, the study encourages the use of animal manure available to the farming community to produce vermicompost and use it in agriculture. Further use of different combinations of organic wastes may help to improve the quality of the product which has further application in crop production.

Conclusion
The present encourages the utility of locally available organic sources like organic municipal solid wastes, farm wastes and animal manures for production of vermicompost in developing countries like Ethiopia. This could be a good source of organic manure for farmers to reduce the chemical fertilizers and lead to sustainable agriculture. Production of compost or vermicompost from organic wastes can be considered as an entrepreneurship for the youth and farmers, thereby an economic and ecofriendly method can be developed. Further studies can be conducted to develop different integrated soil fertility management practices using the khat waste in combination with organic wastes available to the farmers.

Compliance with ethical standards
Conflict of interest The authors declare that there is no conflict of interest.
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