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Effects of unripe apple polyphenols on the expression of matrix metalloproteinase-1 and type-1 procollagen in ultraviolet irradiated human skin fibroblasts

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Abstract

Bioassay-guided isolation of an aqueous ethanolic extract from the unripe fruits of Malus pumila cv. Fuji using matrix metalloproteinase-1 (MMP-1) inhibition and type-1 procollagen inducing assays led to isolation of eight phenolic compounds, reynoutrin (1), quercitrin (2), isoquercitrin (3), phloridzin (4), phloretin-2′-O-xyloglucoside (5), 5-O-p-courmaroylquinic acid (6), chlorogenic acid methyl ester (7), and chlorogenic acid (8). The structures were established on the basis of nuclear magnetic resonance and mass spectroscopic data interpretations. All isolates were evaluated for their inhibitory effects on MMP-1, and compounds 1, 2, and 6 exhibited potent inhibitory effects on MMP-1 with IC50 values of 1.3±0.1, 1.4±0.1, and 3.2±0.6 μM, respectively. When evaluated for their effects on type I procollagen synthesis on human fibroblast cells, compounds 1 and 3 were respectively found to display potent activity for induction of type-1 procollagen by 78.5 and 78.6% at the tested concentration of 12.5 M.

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Authors and Affiliations

  1. Department of Oral Pathology, School of Dentistry, Kyungpook National University, Daegu, 700-412, Republic of Korea

    Eui Kyun Park & So Ra Ahn

  2. Team of Product Development, Institute for Korea Traditional Medicinal Industry, Gyeongsan, 712-749, Republic of Korea

    Dong-Hee Kim

  3. Department of Life Science and Biotechnology, Dong-Eui University, Busan, 614-714, Republic of Korea

    Eun-Woo Lee, Hyun Ju Kwon & Byung Woo Kim

  4. Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan, 614-714, Republic of Korea

    Eun-Woo Lee, Hyun Ju Kwon, Byung Woo Kim & Tae Hoon Kim

  5. Department of Herbal Medicinal Pharmacology, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Tae Hoon Kim

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  1. Eui Kyun Park
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  2. So Ra Ahn
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  3. Dong-Hee Kim
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  4. Eun-Woo Lee
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  5. Hyun Ju Kwon
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  6. Byung Woo Kim
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  7. Tae Hoon Kim
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Correspondence to Tae Hoon Kim.

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Park, E.K., Ahn, S.R., Kim, DH. et al. Effects of unripe apple polyphenols on the expression of matrix metalloproteinase-1 and type-1 procollagen in ultraviolet irradiated human skin fibroblasts. J Korean Soc Appl Biol Chem 57, 449–455 (2014). https://doi.org/10.1007/s13765-014-4128-7

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  • DOI: https://doi.org/10.1007/s13765-014-4128-7

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