New acetogenin katsuurallene from Laurencia saitoi collected from Katsuura, Japan

We examined the chemical constitution of the red alga Laurencia saitoi Perestenko, collected from Katsuura, Boso Peninsula, Chiba Prefecture, Japan. This specimen produced a new polyhalogenated acetogenin, named katsuurallene (1), which structure was determined by the spectral methods, along with known diterpene, deoxyparguerol (2) and triterpene, thyrsiferol (3). In this paper we describe the structural elucidation of katsuurallene together with some biological activities.


Introduction
Red algae of the genus Laurencia (Rhodomelaceae, Ceramiales) are the most intensively studied of all algal genera. More than 1000 secondary metabolites with intriguing skeletal structures, including about 800 halogenated compounds, were reported from this unique genus [1][2][3][4]. New halogenated metabolites are being discovered from Laurencia [5,6], which seem to be an endless source of novel compounds.
The Laurencia species, from which halogenated metabolites have been isolated, possess "corps en cerise" in both superficial cortical cells and trichoblast cells. "Corps en cerise", an unusually swollen refractile inclusion, is recognized as the site of synthesis and/or storage of halogenated compounds [7]. On the other hand, some species without "corps en cerise" produce no halogenated compound. To date, the chemical constitution of 22 species of Japanese Laurencia with "corps en cerise", including 7 taxonomically undescribed species, have been investigated [8][9][10][11].
As part of our additional studies of the chemical diversity in the Japanese Laurencia species, we examined the chemical composition of Laurencia spp. from Katsuura, Boso Peninsula, Chiba Prefecture. Boso Peninsula is situated in the southeast side of Kanto area on Honshu, the largest island of Japan. Its coastline faces Tokyo Bay to the west and Pacific Ocean to the east and south.

Chemical composition of L. saitoi
The EtOAc-soluble fraction was subjected to a combination of column and preparative thin-layer chromatography to yield a halogenated acetogenin, named katsuurallene (1), along with two known terpenoids, deoxyparguerol (2) and thyrsiferol (3).
Katsuurallene ( [10]. Since the IR spectrum revealed no hydroxy and carbonyl absorptions, the two oxygen atoms in 1 were assumed to be involved in ether linkages. Detailed analysis of the 1 H-and 13 C-NMR spectra, as well as HMQC and 1 H-1 H COSY spectra, led to the partial structure 1a (Fig. 2) for katsuurallene (1). In 1a, the oxygen atoms at C-4, C-7, C-9, and C-13 were verified based upon the chemical shift values of the pertinent carbons at 73.79 (C-4), 76.05 (C-9), 77.94 (C-7), and 83.79 (C-13), respectively. Moreover, the substituent at C-6 and C-10 were proven to be chlorine atom by the chemical shifts at 62.83 (C-6) and 61.87 (C-10). This was also confirmed by observation of the halogen-induced 13 C isotope shifts [15] in the 13 C-NMR spectrum. Therefore, the remaining bromine atom is attached to C-12.
The 13 C-NMR spectrum showed that there were no other double bonds apart from those of the bromoallene moiety, and therefore katsuurallene (1), having four degrees of unsaturation, must be composed of two oxide rings. In the HMBC spectra of 1, the longrange correlations between H-4/C-7, H-9/C-13 and H-13/C-9 (Table 1) were observed. This meant that two ether rings must be formed between C-4 and C-7 and between C-9 and C-13, leading to a planar structure 1 for katsuurallene.  The relative stereochemistry was partly determined as follows. In the NOESY spectrum of 1, the nuclear Overhauser effect was observed between H-9 and H-13, thus indicating that both H-9 and H-13 have axial configurations on a tetrahydropyran ring with a chair-like conformation (Fig. 3). Furthermore, in the NMR spectrum (in C 6 D 6 ) of 1 (Table 1, footnote), the H-12 showed the coupling constants of J 12,13 = 10.9 Hz, J 11a,12 = 11.9 Hz and J 11b,12 = 4.6 Hz, which are typical axial/axial, axial/ axial, and axial/equatorial coupling constants, respectively, indicating that the H-12 has axial configuration (equatorial bromine atom) on a tetrahydropyran ring. On the other hand, the H-9 showed the coupling constant of J 9,10 = 2.3 Hz, which is a typical equatorial/axial coupling constant, indicating that the H-10 has an equatorial configuration (axial chlorine atom) on a tetrahydropyran ring.
The relative configuration on the oxolane ring was also determined by the NOESY spectrum. The cis-relationship of the substituents at C-6 and C-7 was shown by a NOE correlation between H-6 and H-7. H-6 was further correlated to Ha-5. On the other hand, Hb-5 was correlated to H-4, thus indicating the trans-relationship between H-4 and H-7 (Fig. 3).
In view of the positive sign of the optical rotation of 1, the absolute configuration of the bromoallene moiety was suggested S-configuration, according to Lowe's rule [16,17], though a few exceptions to Lowe's rule were reported in the case of microcladallenes [18] and also (E)-and (Z)-9-epi-omaezallene [10]. Consequently, the structure of katsuurallene would be represented by formula 1.

Conclusion
As described above, the specimen of Laurencia saitoi from Katsuura contained a new halogenated acetogenin, katsuurallene (1), as a characteristic major metabolite, along with deoxyparguerol (2) and thyrsiferol (3). On the other hand, the specimens from Teuri Island [23][24][25][26][27] and Suttsu [28] in Hokkaido contained diterpenes and triterpenes as characteristic metabolites. Since L. saitoi Perestenko has passed under the name L. obtusa (Hudson) Lamouroux in Japan [29], the former specimen from Teuri Island was first reported as L. obtusa. Three Chinese specimens of L. saitoi have also been examined. The specimen collected from the coast of Yantai, Shandong Province, produced several pargueranediterpenes and two triterpenes thyrsiferol and thyrsiferyl 23-acetate [30], which are very similar to the metabolites of the specimens from Hokkaido. However, the specimen collected from the coast of Rongcheng, northern Shandong Province, produced halogenated chamigrane-, bisabolane-and laurane-sesquiterpenes [31]. And the specimen collected from Hainan coastlines produced halogenated snyderane-sesquiterpenes [32]. The difference in chemical composition of the specimens of L. saitoi strongly requires doing taxonomical reexamination.

Plant material
Laurencia saitoi Prestenko was collected from the coast of Yoshio (35°8′N, 140°17′E), Katsuura, Boso Peninsula, Chiba Prefecture, on 18 May 2018. The voucher specimen has been deposited in the Herbarium of the Coastal Branch of Natural History Museum and Institute, Chiba (CMNH).

Extraction and isolation of L. saitoi
The algal sample (58.0 g dry weight) was extracted twice with MeOH. The resulting MeOH solution was concentrated in vacuo and partitioned between EtOAc and H 2 O. The EtOAc layer was washed with water, dried over dry Na 2 SO 4 and evaporated to leave an oily substance. The EtOAc-soluble extract (545 mg) was then fractionated by Si gel column chromatography with a step gradient (hexane and EtOAc). A portion (120 mg) of the fraction (158 mg) eluted with hexane-EtOAc (4:1) was further subjected to prep. TLC with toluene to yield a crude substance which was purified by prep. TLC with hexane-EtOAc (9:1) to give katsuurallene (1) (19.6 mg). A portion (85 mg) of the fraction (99 mg) eluted with hexane-EtOAc (1:1) was subjected to prep. TLC with hexane-EtOAc (1.5:1) to yield a crude substance which was further subjected to prep. TLC with toluene-EtOAc (2:1) to yield two fractions. The less polar fraction (25 mg) was then purified by prep. TLC with CHCl 3 -MeOH (95:5) to give deoxyparguerol (2) (6.3 mg). The polar fraction (30 mg) was purified by prep. TLC with CHCl 3 -MeOH (95:5) to give thyrsiferol (3) (7.7 mg).   Fig. S8) were found to be identical to those previously reported [33].

Biological activity 3.4.1 Insect repellent assay
The repellent activities of some isolated compounds against the maize weevils Sitophilus zeamais were evaluated using the filter paper impregnation method as previously described [35]. The numbers of adult beetles present in each Petri dish were recorded after 24 h of exposure. Each treatment was repeated three times. Pyrethrin standard was used as a positive control.

Growth inhibition assay
The wild-type Arabidopsis seeds (Col-0) were immersed in 70% ethanol for 5 min and then 1.5% NaClO with Tween 80 for 5 min. Seeds were subsequently rinsed with distilled water, and then soaked in 0.1% agar solution for several hours at 4 °C. Surface sterilized seeds were grown in half-strength Murashige and Skoog (MS) medium supplemented with 0.8% agar and 1% sucrose for 1 week at 22 °C. For the plant growth assay, well-grown seedlings were selected and transferred to 12-well plate containing 1/2 MS medium with 1% sucrose. Test samples were dissolved in DMSO and prepared to a final concentration of 1 mg/mL (less than 1% DMSO). After the treatment of samples, the plates were incubated on a rotary shaker for 4 days at 22 °C under light-dark cycle conditions (12L:12D). The growth of the seedlings was estimated by the individual weights. All the experiments were performed three times.

Antioxidant assay
An aliquot of antioxidant Trolox or compounds diluted with ethanol (20 μL) was mixed with the 80 μM Tris-HCl buffer (pH 7.4) and then added to 100 μL of 200 μM 2,2-diphenyil-picrylhydrazyl (DPPH) (Alfa Aesar) in ethanol. The mixture was shaken vigorously and left to stand for 30 min at room temperature in the dark. The absorbance at 515 nm by DPPH was measured by UV-Vis spectrophotometer.

Brine shrimp assay
A bioassay of toxicity toward brine shrimp was performed as described in the literature [36]. Briefly, the compounds dissolved in ethanol were made up to 5, 10 and 50 μg/mL in artificial seawater. Serial dilution was made in the wells of 24-well microplates (Iwaki, Asahi Techno Glass Co., Tokyo, Japan) in triplicate in artificial seawater (2 mL). Brine shrimp eggs obtained locally (Japan Pet Design Co., Ltd., Tokyo, Japan) were hatched in artificial seawater at 25 °C. After 48 h, a suspension of nauplii containing 10-20 organisms (100 µL) was added to each well and incubated at 25 °C for 24 h and the numbers of non-motile and total nauplii in each well were counted in turn.

Antimicrobial assay
Antibacterial bioassays were carried out using Escherichia coli NBRC-3972 strain. Organism was precultured in LB medium for 2 days. The turbidity of the culture was adjusted to 10 7 cells/mL using hemocytometer. Then 0.2 mL of the precultured bacterial suspension was used to seed LB agar plate. Paper discs (6 mm; ADVANTEC Toyo, Tokyo, Japan) impregnated with various amounts of the respective pure compounds were placed on the seeded agar plates and the diameters of the inhibitory zones were measured after the plates were incubated at 37 °C for 2 days.