A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes

6α-Hydroxylup-20(29)-en-3-on-28-oic acid (1), a natural triterpenoid, was found to possess the ability in a dose-dependent manner inhibiting hormone-induced adipocyte differentiation in 3T3-L1 preadipocytes, and restoring glucose consuming ability in dexamethasone (DXM)-induced insulin resistant 3T3-L1 adipocytes. Compound 1 was also found to ameliorate DXM-induced adipocyte dysfunction in lipolysis and adipokine secretion. Mechanistic studies revealed that 1 inhibited adipocyte differentiation in 3T3-L1 preadipocytes via down-regulating hormone-stimulated gene transcription of peroxisome proliferator-activated receptor γ and CCAAT-enhancer-binding protein alpha which are key factors in lipogenesis, and restored DXM-impaired glucose consuming ability in differentiated 3T3-L1 adipocytes via repairing insulin signaling pathway and activating down-stream signaling transduction by phosphorylation of signaling molecules PI3K/p85, Akt2 and AS160, thus leading to increased translocation of glucose transporter type 4 and transportation of glucose.


Introduction
Obesity and obesity-induced insulin resistance increases the likelihood of major diseases referred as metabolic syndrome, such as heart disease, type 2 diabetes, stroke, etc. [1,2]. Hypertrophic adipocytes in obesity release excess lipolytic products into blood, resulting in pathological changes and functional damage to organ tissues including pancreas, and inactivation of insulin receptor (IR), thus increase the risk of insulin resistance and type 2 diabetes [3,4]. In addition, adipose tissue in obesity secrets elevated level of proinflammatory adipokines such as leptin, tumor necrosis factora (TNF-a) and interleukins (ILs) but lowered level of antiinflammatory cytokine such as adiponectin, resulting in metabolic disorder and chronic inflammation of the body [5,6]. Abnormally secreted adipokines may also reduce insulin sensitivity of target tissues by impairing insulin signaling transduction [6,7]. Insulin resistance is characterized by reduced glucose uptake by target tissues or cells (adipose, muscle, liver) owing to impaired insulin signaling transduction and/or glucose transporting ability. In adipocyte, inactivation of insulin signaling molecules such as IR, insulin receptor substrate (IRS), PI3K and Akt, especially the down-stream PI3K and Akt, reduces the translocation of intracellular glucose transporter 4 (GLUT4) to plasma membrane and consequently impairs the glucose transporting ability of the cell [8]. Chinese herbal medicines contribute greatly to the research and development of new drugs. Structurally diverse compounds with bioactivities in reducing blood lipid and glucose level, or prevention of cardiovascular and metabolic diseases have been discovered, such as stilbenoids from Polygonum multiflorum and flavonoids from Crataegus pinnatifida [9,10]. In our attempt to search natural components with hypoglycemic or hypolipidemic potential, 6a-hydroxylup-20(29)-en-3-on-28-oic acid (1) -a lupane type triterpenoid from Viburnum odoratissimumwas found to be able to enhance glucose uptake in dexamethasone-induced insulin resistant 3T3-L1 adipocytes and HepG2 cells [11]. Further investigations were then conducted and here we give a detail report for the effects and working mechanisms of 1 on hormone-stimulated adipogenesis, and dexamethasone-induced insulin resistance and adipocyte dysfunction. Rosiglitazone, which is an insulin sensitizer working as agonist of peroxisome proliferatoractivated receptor c, was used as the drug control.

Compound 1 Inhibited ''Hormone Cocktail''-Induced Adipocyte Differentiation and Lipogenesis in 3T3-L1 Preadipocytes
In an 48-h proliferation assay, 1 at 20, 10 or 5 lM did not affect the viability of 3T3-L1 preadipocytes (Fig. 1), but in ''hormone cocktail''-induced adipocyte differentiation, 1 dose-dependently reduced the staining of cells by Oil red O, indicating inhibited adipocyte differentiation. Differentiation ratio of 3T3-L1 preadipocytes in the presence of 20, 10 or 5 lM of 1 was remarkably decreased from 100 % of the control to 36.8, 57.1 or 81.7 % (P \ 0.001 each), respectively (Fig. 2). In this experiment, rosiglitazone at 20 lM enhanced adipocyte differentiation as expected. Triglyceride (TG) is the indicator of lipogenesis in fat cell. Synthesis of TG by cultural fat cells relies on the decomposition of glucose to generate free fatty acids (FFAs). Glucose utilization and FFA production are therefore indirect indicators for lipogenesis. As shown in Fig. 3, glucose remaining in culture medium of 3T3-L1 cells with differentiation induction was much less than that in medium of cells without induction (6.0 mM vs. 13.6 mM, P \ 0.001), implying stimulated glucose utilization and lipogenesis in adipocyte differentiation.Unlike rosiglitazone which stimulated the glucose utilization and TG synthesis, 1 at 20 or 10 lM increased the glucose remaining (P \ 0.01 or 0.05), as a result, reduced FFA production (P \ 0.01 each) and cellular TG content (P \ 0.001 or 0.01) of induced cells, showing inhibitory effect on ''hormone cocktail''-induced adipocyte differentiation and lipogenesis. PPARc and C/EBPa are two key transcriptional factors in adipocyte differentiation and lipogenesis, both express in very low level in preadipocytes but are significantly upregulated in the process of adipocyte differentiation [12]. In this study, gene transcription of PPARc and C/EBPa in 3T3-L1 cells were remarkably stimulated by ''hormone cocktail'' (P \ 0.001 and 0.0001 vs. cells not induced), the addition of rosiglitazone (a PPARc agonist) further stimulated the expression of the two genes, but the addition of 1 (20 or 10 lM) into the differentiation medium caused marked reduction in cellular mRNA levels of PPARc (P \ 0.05 vs. induced cell control) and C/EBPa (P \ 0.01 or 0.05 vs. induced cell control) (Fig. 4). As a result, fatty acid synthase (FAS), the key enzyme catalyzing the synthesis of FFA [13], was suppressed by 1 (P \ 0.001) (Fig. 3). In this study, 1 inhibited hormone-stimulated adipocyte differentiation and lipogenesis through down-regulating gene expression of PPARc and C/EBPa, showing potential to deal with obesity and obesity-related health problems.

Compound 1 Restored Glucose Consuming Ability and Alleviated Dysfunction in Dexamethasone-Induced Insulin Resistant 3T3-L1 Adipocytes
As mentioned above, obesity is a primary cause for development of insulin resistance, and insulin resistance always accompanies with metabolic disorder. In this study, effect of 1 on improvement of glucose consuming ability and metabolic disorder was investigated in insulin resistant 3T3-L1 adipocytes. Dexamethasone (DXM), which impairs glucose transporting ability and causes insulin resistance and metabolic disorder in adipose tissue and adipocytes [14,15], was used to induced insulin resistant state of the cells.
Reduced glucose uptake is the character of insulin resistance. In our study, 3T3-L1 adipocytes in the presence of 1 lM DXM consumed much less glucose within 48 h than cells in the absence of DXM (P \ 10 -5 ), indicating that insulin resistant state in 3T3-L1 adipocytes was successfully induced by DXM treatment. 1 at 20 and 10 lM enhanced the glucose consuming ability of DXM-treated 3T3-L1 adipocytes in a dose-dependent way (P \ 10 -5 and 10 -4 ) like rosiglitazone did, implying that 1 restored the glucose transport ability of the cells (Fig. 5a).
Oversupplied lipolytic products contribute to insulin resistance. In this study, effect of 1 on lipolysis in DXMtreated 3T3-L1 adipocytes was monitored by measuring the glycerol concentration in culture medium. Result showed that, 3T3-L1 adipocytes in the presence of 1 lM DXM released much more glycerol into medium within 48 h than cells in the absence of DXM (P \ 0.001), indicating stimulated lipolysis by DXM. Compound 1 and rosiglitazone at 20 lM reduced the glycerol concentration in medium (P \ 0.05) (Fig. 5b), showing inhibitory effect on DXM-stimulated lipolysis in 3T3-L1 adipocytes. Overexpressed proinflammatory cytokines (TNF-a, IL-6, leptin, etc.) and reduced expression of anti-inflammatory cytokine (adiponectin) are responsible for chronic inflammation and the development of insulin resistance [16]. It was reported that obese mice lacking either TNF-a or IL-6 show protection against developing insulin resistance, while chronic exposure to TNF-a, IL-6 or leptin activates PTP1B gene and reduces the expression of IRS proteins in myocytes or adipocytes of mice [17][18][19]. The anti-inflammatory cytokine adiponectin is specifically expressed by adipocyte and directly sensitizes the body to insulin [20]. Lack of adiponectin causes obesity and contributes to insulin resistance, type 2 diabetes, and the metabolic syndrome, while administration of adiponectin improves insulin resistance [21,22]. Our study showed that, 3T3-L1 adipocytes in the presence of 1 lM DXM secreted more TNF-a (P \ 0.05) and IL-6 (P \ 0.01), but less leptin (P \ 0.05) and adiponectin (P \ 0.05) (Fig. 6) into medium than DXM-free ones, indicating severe disorder in cytokine secretion was induced by DXM treatment. Compound 1 at 20 lM significantly reduced the secretion of TNF-a (P \ 0.05), IL-6 (P \ 0.001) and leptin (P \ 0.001), and elevated the secretion of adiponectin (P \ 0.05) in DXM treated 3T3-L1 adipocytes, showing regulatory effect on DXM-induced adipocyte dysfunction   Fig. 6) that was different from rosiglitazone. Rosiglitazone at 20lM remarkably increased the secretion of TNF-a and IL-6 in 3T3-L1 adipocytes.

Compound 1 Repaired Insulin Signaling Pathway
and Activated PI3K/Akt2/AS160 Signaling in DXM-Induced Insulin Resistant 3T3-L1 Adipocytes In mature adipocytes, glucose uptake is carried out by GLUT4 which is driven by insulin signaling transduction that is activated by sequential posphorylation of insulin signaling proteins, including IR, IRS1, PI3K and Akt2. Phosphorylated Akt2 then stimulates the translocation of intracellular GLUT4 vesicles to plasma membrane for functioning as glucose transporters [23]. Inhibition of signaling proteins leads to inhibited GLUT4 translocation and weakened glucose transporting ability of cells [24]. In which, the phosphorylation of down-stream PI3K/p85 and Akt2 plays a key role in GLUT4 translocation [25]. AS160, a substrate of Akt2, also regulates GLUT4 trafficking in adipocytes. Nonphosphorylated AS160 binds to intracellular GLUT4 vesicles and inhibits GLUT4 translocation, and AS160 phosphorylation overcomes this inhibitory effect [26]. Effects of 20 lM 1 on expression and/or phosphorylation of IR, IRS1, PI3K, Akt2, GLUT4 and AS160 in DXM-induced insulin resistant 3T3-L1 adipocytes were investigated. Result showed that, in DXMtreated 3T3-L1 adipocytes, expression of IR-a, IRS1, PI3K/p85, Akt2 and GLUT4 was suppressed (Fig. 7a), phosphorylation of down-stream proteins PI3K/p85, Akt2 and AS160 was obviously inhibited, while the phosphorylation of upper-stream proteins IR-b and IRS1 kept unchanged (Fig. 7b). The addition of 20 lM 1 to DXMtreated 3T3-L1 adipocytes elevated the expression of IR-a, IRS1, Akt2 and GLUT4 (Fig. 7a), and increased the phosphorylation of PI3K/p85, Akt2 and AS160, but did not affect the phosphorylation level of IR-b and IRS1, (Fig. 7b), showing activity similar to rosiglitazone. Result suggested that, DXM impaired insulin signaling pathway and reduced glucose consuming ability in 3T3-L1 adipocytes not by inhibiting upper-stream IR signaling transduction, but by directly inhibiting the down-stream PI3K/ Akt2/AS160 signaling, thus leading to reduced GLUT4 translocation and glucose transporting ability. Compound 1 ameliorated DXM-induced impairment on insulin signaling pathway, and stimulated the phosphorylation of PI3K/ Akt2/AS160, showing potential to deal with DXM-induced insulin resistance.

Cell Line and Cell Culture
Murine preadipocyte cell line 3T3-L1 was kindly provided by Prof. WF Fong of Hong Kong Baptist University, and were grown in Dulbecco's Modified Eagle's Medium containing 4.5 g/L of glucose and 10 % fetal bovine serum (Gibco, Rockville, MD, USA), at 37°C, saturate humidity and 5 % CO 2 .

Cell Proliferation Assay
3T3-L1 preadipocytes in 100 lL growth medium were seeded in 96-well plates at a density of 5000 cells per well. Twenty-four hours later, different concentrations of 1 were added to the medium. After 48 h of incubation, cell viability was measured by using CCK-8 assay kit.

Induction for Adipocyte Differentiation
3T3-L1 preadipocytes were induced for adipocyte differentiation by the ''hormone cocktail'' [11]. Briefly, the 4th generation cells were grown in growth medium until confluence. Cells were induced for adipocyte differentiation in growth medium containing 0.5 mM IBMX, 1 lM DXM and 10 lg/mL insulin for 2 days, and in growth medium containing 10 lg/mL insulin for another 2 days, and then in drug free growth medium for 4 days. Medium was replaced every 2 days. To investigate the effect of 1 on ''hormone cocktail''-induced adipocyte differentiation, 1 of 20, 10 or 5 lM, or rosiglitazone of 20 lM was added together with the ''hormone cocktail'' on days 1-4.

Detection of Adipocytes
Differentiated adipocytes were detected by staining the cellular lipid red with Oil red O [27]. After taking pictures under an inverted microscope, cellular Oil red O was dissolved with certain volume of isopropanol. Absorbance which is positively correlated to the rate of successful differentiation was read at 492 nm.

Glucose Utilization, FFA and TG Synthesis in Adipocyte Differentiation
In 12-well plates, 3T3-L1 preadipocytes were induced for adipocyte differentiation for 8 days as described above, with 1.5 mL medium per well. 1 of various concentrations or rosiglitazone of 20 lM was added into the differentiation medium on days 1-4. Supernatant medium on days 6 was collected for measurement of glucose and FFA by using glucose assay kit and FFA detection kit, respectively. Cells on days 8 were washed with ice-cold PBS trice and then repeatedly frozen and thawed at -80/ 37°C until the cells were fully disrupted. After addition of 100 uL of PBS, cells were shaved by Cell Scraper and the mixed liquor was collected and centrifuged for 5 min at 12.5 9 10 3 g. Supernatant was collected for assessment of TG concentration by using the kit. Intracellular TG content was normalized to the concentration of total protein (TP) measured by using Enhanced BCA Protein Assay Kit.

Measurement of Cellular FAS Content
In 6-well plates, 3T3-L1 preadipocytes were induced for adipocyte differentiation for 8 days as described above. Cells were washed with 2 mL PBS, lysed with 150 lL cell lysis buffer on ice for 2 min. Cell lysate was collected by centrifuge at 13.5 9 10 3 g for 5 min at 4°C. Content of FAS was measured by using the kit, intracellular FAS level was normalized to the content of total protein measured by using the protein assay kit.  adiponectin, TNF-a and IL-6 were measured by using the kits.

RT-PCR Analysis
One microgram of total RNA was subjected to RT reaction using Bestar qPCR RT Kit (DBI Bioscience, Ludwigshafen, Germany). RT reaction product was then amplified by PCR with the gene specific primers listed in Table 1 (Sangon Biotech, Shanghai, China) and Bestar Ò SybrGreen qPCRmaster Mix (DBI) at 94°C for 2 min, 94°C for 20 s, 58°C for 20 s, 72°C for 20 s, and 40 cycles. The gene expression levels were analyzed by a StrataGene Mx3000P QPCR Real-Time PCR System (Agilent) and normalized using b-actin. Results were Mean ± SD from three independent experiments.

Western-Blot Analysis
Thirty micrograms of total protein were separated in 10 % SDS-PAGE and electro-transferred to PVDF membranes. After blocking with 5 % BSA, membranes were incubated with primary antibodies in 2.5 % BSA, 0.3 % Tween-20 for 2 h at room temperature followed by corresponding secondary antibodies for another 1.5 h at room temperature. Protein bands were visualized by ECL.

Statistical Analysis
All data are representative of three independent experiments. Values are expressed as mean ± SD. Statistical analyses were conducted using One-Way ANOVA. A value of P \ 0.05 was considered to indicate statistical significance.