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The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence

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Abstract

Mesenchymal stromal cells (MSC) are promising options to cellular therapy to several clinical disorders, mainly because of its ability to immunomodulate and differentiate into different cell types. Even though MSC can be isolated from different sources, a major challenge to understanding the biological effects is that the primary cells undergo replicative senescence after a limited number of cell divisions in culture, requiring time-consuming and technically challenging approaches to get a sufficient cell number for clinical applications. Therefore, a new isolation, characterization, and expansion is necessary every time, which increases the variability and is time-consuming. Immortalization is a strategy that can overcome these challenges. Therefore, here, we review the different methodologies available to cellular immortalization, and discuss the literature regarding MSC immortalization and the broader biological consequences that extend beyond the mere increase in proliferation potential.

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Funding

This work was supported by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, EDITAL FAPERGS 07/2021—PROGRAMA PESQUISADOR GAÚCHO—PqG (21/2551-0001947-6) and RITES (22/2551-0000385-0); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)MS-SCTIE-Decit/CNPq nº 12/2018 (441575/2018-8), MS-SCTIE-DECIT-DGITIS-CGCIS/CNPq nº 26/2020 (442586/2020-5), and National Institute of Science and Technology in 3D printing and Advanced Materials Applied to Human and Veterinary Health-INCT _3D-Saúde (406436/2022-3). Luana S. Lenz was a recipient of a Postdoc fellowship from (UFCSPA EDITAL Nº 07/2021). Márcia R. Wink is recipient of a Level 1 research productivity fellowship from the CNPq.

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  1. Laboratório de Biologia Celular, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, 90050-170, Brazil

    Luana Suéling Lenz & Márcia Rosângela Wink

  2. Departamento de Ciências Básicas da Saúde (DCBS), Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, 90050-170, Brazil

    Márcia Rosângela Wink

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MRW: first conceptualized the study and revised the work. LSL: performed literature searches, wrote the manuscript, and drew figures. Both authors read and approved the final manuscript.

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Correspondence to Márcia Rosângela Wink.

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Lenz, L.S., Wink, M.R. The other side of the coin: mesenchymal stromal cell immortalization beyond evasion of senescence. Human Cell 36, 1593–1603 (2023). https://doi.org/10.1007/s13577-023-00925-3

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