Abstract
The genetic transformation is a genotype specific phenomena in potato, which complicates the trait specific genetic manipulations and mandates optimisation of protocol for each cultivar. In the present study, heat shock treatment was used to develop genotype independent Agrobacterium-mediated genetic transformation protocol of potato. Initially six cultivars of potato namely, ‘Kufri Chipsona 1’ (CS-1), ‘Kufri Chipsona 2’ (CS-2), ‘Kufri Pukhraj’ (KP), ‘Kufri Khyati’ (KK), ‘Kufri Jyoti’ (KJ) and ‘Kufri Chandramukhi’ (KCM), were tested for genetic transformation efficiencies using previously optimised Agrobacterium mediated T-DNA delivery protocol and the outcome was highly genotype dependent. Based on the response, cultivars were grouped as ‘high’ (CS-1, CS-2, KK) and ‘low’ (KP, KCM, KJ) genetic transformation efficiency cultivars. Further, the attempts were made to understand the biochemical basis of variation in genetic transformation efficiencies of these cultivars. It was noteworthy that in ‘low genetic transformation efficiency’ cultivars, the level of antioxidant enzymes and phenols was significantly higher than ‘high genetic transformation efficiency’ cultivars. In order to optimise a genotype independent protocol, explants from all the cultivars (both groups) were subjected to heat shock treatment (30–50 °C; 15–45 min) immediately before infection. The heat shock treatment at 40 °C for 15 min resulted in a significant increase in transient GUS expression, callogenesis and regeneration of transgenic shoots. Using this approach, transgenic shoots were recovered from all the cultivars. Following the heat shock treatment, levels of antioxidant enzymes and phenols were significantly affected, thus it seems that cultivars with stronger defense system are likely to be more difficult for A. tumefaciens-mediated genetic transformation.
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Abbreviations
- BA:
-
6-Benzyladenine
- FW:
-
Fresh weight
- GA3:
-
Gibberellic acid
- GUS:
-
β-Glucuronidase
- HS:
-
Heat shock
- CS1:
-
Kufri Chipsona 1
- CS2:
-
Kufri Chipsona 2
- KP:
-
Kufri Pukhraj
- KK:
-
Kufri Khyati
- KJ:
-
Kufri Jyoti
- KCM:
-
Kufri Chandramukhi
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Acknowledgements
Authors are thankful to TIFAC-CORE, Thapar Institute of Engineering & Technology for providing facilities for conducting experiments. Authors also thank the Council of Scientific and Industrial Research, Extramural Research Division, New Delhi for funding this study through the Project 38(1465)/18/EMR-II.
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This study has received funding from Council of Scientific and Industrial Research, Extramural Research Division, New Delhi through the project 38(1465)/18/EMR-II.
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AK conducted the experiments, compiled data, analyzed the results and wrote the initial draft of the manuscript. AK and AK conceived and designed the experiments. MSR helped in statistical analysis and molecular studies. AK finalized the manuscript.
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Kaur, A., Reddy, M.S. & Kumar, A. Heat shock enhanced Agrobacterium tumefaciens mediated T-DNA delivery to potato (Solanum tuberosum L.). J. Plant Biochem. Biotechnol. 31, 853–863 (2022). https://doi.org/10.1007/s13562-021-00762-1
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DOI: https://doi.org/10.1007/s13562-021-00762-1