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An improved method for DNA isolation from polyphenols rich leaf samples of Annona squamosa L.

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Abstract

A reliable and efficient method for isolating Annona squamosa L. genomic DNA, free from polyphenols and polysaccharides has been developed. Different methods involving use of CTAB and SDS with or without modifications were used. A CTAB based extraction method which uses diatomite to remove polyphenols and polysaccharides proved to be the best. This method allowed recovery of good quality DNA in sufficient quantity suitable for complete digestion by restriction endonucleases and amplifiable in polymerase chain reaction as compared to other methods.

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Fig. 1
Fig. 2

Abbreviations

CTAB:

Cetyl trimethylammonium bromide

TE buffer:

Tris EDTA Buffer

EDTA:

Ethylenediaminetetraacetic acid

RAPD:

Random Amplified Polymorphic DNA

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Acknowledgement

The financial support provided to the first author, Rakhi Nagori, under the Women Scheme - A by DST, New Delhi, is gratefully acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, 313001, India

    Rakhi Nagori & Sunil Dutta Purohit

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  1. Rakhi Nagori
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  2. Sunil Dutta Purohit
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Correspondence to Sunil Dutta Purohit.

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Nagori, R., Purohit, S.D. An improved method for DNA isolation from polyphenols rich leaf samples of Annona squamosa L.. J. Plant Biochem. Biotechnol. 21, 140–143 (2012). https://doi.org/10.1007/s13562-011-0075-0

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  • DOI: https://doi.org/10.1007/s13562-011-0075-0

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