Skip to main content
SpringerLink
Log in

Green synthesis of silver nanoparticles using Artemisia persica extract and evaluation of their activity on onion gray rot fungus

  • Original Article
  • Published:
Biomass Conversion and Biorefinery Aims and scope Submit manuscript

Abstract

Gray rot is a severe onion disease caused by the Botrytis fungus. Currently, green synthesis of silver nanoparticles (Ag NPs) using plants is being explored as an alternative to chemical poisons. In this research, pathogenic fungi were isolated and identified from onions with rotting symptoms. The biosynthesis of Ag NPs was performed using the aqueous extract of Artemisia leaves. The synthesis of Ag NPs was characterized using various techniques such as UV–Vis, FTIR, and TEM. The biosynthesis of Ag NPs was confirmed by observing the color change from light yellow to brown after 15 min at room temperature and dark conditions. The UV spectra revealed that the surface plasmon resonance is at 420 nm. According to TEM analysis, the average size of Ag NPs was approximately 20 nm. The synthesized Ag NPs were then tested against Botrytis fungus. Ten Botrytis strains were isolated, and the S3 isolate from the Islamabad region was selected. In vitro conditions showed that the growth inhibition rate of Ag NPs and Carbendazim poison, alone and in combination, against Botrytis fungus were 53.84%, 100%, and 100%, respectively. Moreover, Ag NPs and Carbendazim poison, alone and in combination, showed 100% shelf life of onions in storage within 3 and 2 months, respectively. Therefore, the synthesized Ag NPs were found to be effective in controlling pathogenic fungi without using fungicides.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11

Similar content being viewed by others

Data availability

All the data generated/analyzed during the study are available with the corresponding author on reasonable request.

References

  1. M Steentiges O Scholten J Vankan 2020 Peeling the onion: towards a better understanding of Botrytis diseases of onion https://doi.org/10.1094/PHYTO-06-20-0258-IA

  2. Araujo E, Alves D, Higashikawa F (2018) Cultivar resistance and chemical, biological and fertilizer treatments for controling Botrytis leaf blight of onion. Trop Plant Pathol 43:160–164. https://doi.org/10.1007/s40858-017-0193-x

    Article  Google Scholar 

  3. Sadeghieh AS, Farzaneh A, Amani F, Audri Sh (2011) Epidemiology of agricultural pesticide poisoning in patients referred to Imam Khomeini and Social Security Hospitals in Ardabil. J Sanit Health 5(3):240–247 ((In Persian))

    Google Scholar 

  4. Kuzmanovska B, Rusevski R, Jankulovska M, Katerina B (2018) Antagonistic activity of Trichoderma asperellum and Trichoderma harzianum against genetically diverse Botrytis cinerea isolates. Chil J Agric Res 78(3):391–400

    Article  Google Scholar 

  5. Zhou H, Yuan X, Zhou H et al (2022) Surveillance of pine wilt disease by high resolution satellite. J For Res 33:1401–1408. https://doi.org/10.1007/s11676-021-01423-8

    Article  Google Scholar 

  6. Karakaş İ, Sağır LB, Hacıoğlu Doğru N (2023) Biological activities of green synthesis silver nanoparticles by Plantago lanceolata L leaves. Biol Pharm Sci 22(02):290–296

    Article  Google Scholar 

  7. Ekhtiari Yingjeh Sh, Ezzatzadeh E (2017) Rapid and biocompatible synthesis of silver nanoparticles using Thymus talashi leaf and evaluation of its antimicrobial activity. Appl Res Chem 11(4):51–59 ((In Persian))

    Google Scholar 

  8. Darbahania R, Norouzian Sham Esbid D, Akbarzadeh KA (2015) Biostosis of gold nanoparticles using Staphylococcus aureus and investigation of its antibacterial and cytotoxicity. Cell Mol Biol 6(21):67–73 ((In Persian))

    Google Scholar 

  9. Nowak B, Krug H, Height M (2011) 120 Years of nanosilver history: implications for policy makers. Am Chem Soc 45(4):1177–1183

    Google Scholar 

  10. Bayda S, Adeel M, Tuccinardi T, Cordani M (2020) The history of nanoscience and nanotechnology: from chemical–physical applications to nanomedicine. Molecules 25(1):112

    Article  CAS  Google Scholar 

  11. Ajitha B, Reddy Y, Reddy P, Jin JH (2016) Role of capping agents in controlling silver nanoparticles size, antibacterial activity and potential application as optical hydrogen peroxide sensor. RSC Adv 42:130–139

    Google Scholar 

  12. Lamsal K, Kim SW, Jung JH, Kim YS, Kim KS, Lee YS (2011) Application of silver nanoparticles for the control of Colletotrichum species in vitro and pepper anthracnose disease in field. Mycobiology 39(3):194–199

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Salari S, Esmaeilzadeh BS, Yousef ZF (2016) The effect of bio-particulate nanoparticles on plant pathogens. J Biosaf 9(1):1–8 ((In Persian))

    Google Scholar 

  14. Mittal AK, Kaler A, Banerjee UC (2012) Free radical scavenging and antioxidant activity of silver nanoparticles synthesized from flower extract of Rhododendron dauricum. Nano Biomed Eng 4:118–124

    Article  CAS  Google Scholar 

  15. Rasheed T, Bial M, Iqbal H, Li C (2017) Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids Surf, B 158:408–415

    Article  CAS  Google Scholar 

  16. Venat O, Iacomi B, Peticila G (2018) Original article in vitro studies of antifungal activity of colloidal silver against important plants pathogens. Not Bot Horti Agrobo 46(2):533–537

    Article  CAS  Google Scholar 

  17. Muthu K, Akilandaeaswari B, Mangala NS (2019) Green synthesis of silver nanoparticles using Polyalthia longifolia stem bark extract and its catalytic reduction of 4-Nitrophenol. Asian J Chem 31(11):2439–2442

    Article  CAS  Google Scholar 

  18. Khatoon N, Ahmad R, Sardar M (2015) Robust and fluorescent silver nanoparticles using Artemisia annua: biosynthesis, characterization and antibacterial activity. Biochem Eng J 102:91–97

    Article  CAS  Google Scholar 

  19. Ouda S (2014) Antifungal activity of silver and copper nanoparticles on two plant pathogens, Alternaria and Botrytis cinerea. Res J Microbiol 9(1):34–42

    Article  Google Scholar 

  20. Baladi N, Zarei G (2021) Biosynthesis of silver nanoparticles by Achillea eriophora DC. extract. J Nanoanalysis 8(3):176–183. https://doi.org/10.22034/jna.002

  21. Mohan SC, Sasikala K, Anand T, Vengaiah P, Krishnaraj S (2014) Green synthesis antimicrobial and antioxidant effects of silver nanoparticles using Canthium coromandelicum leaves extract. Res J Microbiol 9:142–150

    Article  Google Scholar 

  22. Kavosi S, Yaghoubi H (2017) Synthesis of silver nanoparticles by green method using the extract of European marjoram and investigation of its antimicrobial effects. J Cell Mol Res 30(2):161–174 ((In Persian))

    Google Scholar 

  23. Vanjana M, Paulkumar K, Gnanajobitha G, Rajeshkumar S (2014) Herbal plant synthesis of antibacterial silver nanoparticles by Solanum trilobatum and its characterization. Int J Metals. https://doi.org/10.1155/2014/692461

    Article  Google Scholar 

  24. Vidyasagar A, Ritu Raj Patel A, Sudhir KS, Meenakshi S (2023) Green synthesis of silver nanoparticles: methods, biological applications, delivery and toxicity. Mater Adv 4:1831–1849

    Article  CAS  Google Scholar 

  25. Ali M, Kim B, Belfield K, Norman D (2016) Green synthesis and characterization of silver nanoparticles using Artemisia absinthium aqueous extract — A comprehensive study. Mater Sci Eng 58:359–365

    Article  CAS  Google Scholar 

  26. Jafari PM, Afsharzadeh S, Akafi H, Abbasi Sh (2017) Floristic study of rangelands in mountain ridge in Isfahan province Iran. New Find Biolo Sci 4(3):236–245 ((In Persian))

    Google Scholar 

  27. Doosti B, Nabipour F, Haji AA (2019) Green synthesis of silver nanoparticles using aqueous extract of fumitory and investigating its antimicrobial and anti-oxidant properties. Razi J Med Sci 26(6):105–116 ((In Persian))

    Google Scholar 

  28. Khalili H, Baghbani AF (2017) Green synthesis of silver nanoparticles using Artemisia extract and cytotoxicity on colon cancer cell lines. J Ilam Univ Med Sci 25(2):91–101 ((In Persian))

    Google Scholar 

  29. Adly SM, Zarifian SHA, Hosseini Lineage SD (2012) Factors affecting the symility of agricultural operations in Jiroft (case study: onion, potatoes and tomatoes). Agric Econ Dev 4:459–468 ((In Persian))

    Google Scholar 

  30. Mavandadi A, Khaje AJ, Sharif NB (2015) Efficacy of common fungicides in control of tomato gray mold. Sci Technol Greenh Cult 6(24):181–180 ((In Persian))

    Google Scholar 

  31. Chilvers M (2006) Detection and identification of Botrytis species associated with neck rot, scape blight, and umbel blight of onion. Plant Health Progress. https://doi.org/10.1094/PHP-2006-1127-01-DG

  32. Mirzaei S, Goltapeh M, Bakhsh M. 2007. Identification of Botrytis spp. on plants grown in Iran. J Phytopathol, https://doi.org/10.1111/j.1439-0434.2007.01317.

  33. Kaviani N, Asfoori M (2017) Biosynthesis of silver nanoparticles using Artemisia annua. J Biotechnoly Tarbiat Modares Univ 9(1):12–19 ((In Persian))

    Google Scholar 

  34. Heydari R, Rashidpour M, Azadpour M (2016) Green synthesis of silver nanoparticles by aqueous extract of Rosemary and investigation of its antimicrobial properties. Nanomaterials 26(8):99–106 ((In Persian))

    Google Scholar 

  35. Hajian MH, Ghorbanpour M, Abtahi F, Hadian J (2022) Differential effects of biogenic and chemically synthesized silver-nanoparticles application on physiological traits, antioxidative status and californidine content in California poppy (Eschscholzia californica Cham. Environ Pollut 292:118300

    Article  CAS  PubMed  Google Scholar 

  36. Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Ram Kumar Pandian S, Muniyandi J, Hariharan N, Hyun ES (2009) Biosynthesis, purification, and characterization of silver nano particles using Escherichia coli. Colloids Surf, B 74(1):328–335

    Article  CAS  Google Scholar 

  37. Ibrahim E, Luo J, Ahmed T, Wu W, Yan C, Li B (2020) Biosynthesis of silver nanoparticles using onion endophytic bacterium and its antifungal activity against rice pathogen Magnaporthe oryzae. J Fungi (Basel) 6(4):294. https://doi.org/10.3390/jof6040294

    Article  CAS  PubMed  Google Scholar 

  38. Yan F, Li C, Ye X, Lian Y, Wu Y, Wang X (2020) Antifungal activity of lipopeptides from Bacillus amyloliquefaciens MG3 against Colletotrichum gloeosporioides in loquat fruits. Biol Control 146:104281. https://doi.org/10.1016/j.biocontrol.2020.104281

    Article  CAS  Google Scholar 

  39. Hussein MMA, Abo-Elyousr KAM, Hassan MAH et al (2018) Induction of defense mechanisms involved in disease resistance of onion blight disease caused by Botrytis allii. Egypt J Biol Pest Control 28:80. https://doi.org/10.1186/s41938-018-0085-5

  40. Safari MM, Jafari N (2020) Biological control of Botrytis cinerea, the causal agent of rose gray mold disease by antagonistic fungi. Int J Pest Manag. https://doi.org/10.1080/09670874.2020.1807654

    Article  Google Scholar 

  41. Darvish NM, Rezaei NA, Delghan B (2015) Effect of Satureja Khuzestani essence, Reshingari، Carvacrol and Benomil fungicide on growth inhibition of Botrytis cinerea fungi causing gray fruit disease. Agron Agric 17(2):531–541 ((In Persian))

    Google Scholar 

  42. Rama P., Mariselvi P., Sundaram R. and Muthu K. 2023. Eco-friendly green synthesis of silver nanoparticles from Aegle marmelos leaf extract and their antimicrobial, antioxidant, anticancer and photocatalytic degradation activity. Heliyon.1–13. https://doi.org/10.1016/j.heliyon.2023.e16277

  43. Tariq M, Mohammad KN, Ahmed B, Siddiqui MA, Lee J (2022) Biological synthesis of silver nanoparticles and prospects in plant disease management. Molecules 27(15):4754. https://doi.org/10.3390/molecules27154754

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Elmer W, Ma Ch, White J (2018) Nanoparticles for plant disease management. Curr Opin Environ Sci Health 6:66–70

    Article  Google Scholar 

  45. Thangapushbam V, Muthu K (2022) Biosynthesis of silver nanoparticles using Martynia annua and its antimicrobial and cytotoxic activities. Mater Technol 37(14):3174–3183. https://doi.org/10.1080/10667857.2022.2135475

    Article  CAS  ADS  Google Scholar 

  46. Qiu L, Yang H, Lei F, Fan Sh (2014) Studies on the bacteriostatis of nanosilver on the pathogenic fungus Botrytis cinerea from illes plants. Appl Mech Mater 63:653–661

    Google Scholar 

  47. Mathew S, Victorio CP, Sidhi J, Bh BT (2020) Biosynthesis of silver nanoparticle using flowers of Calotropis gigantea (L.) WT Aiton and activity against pathogenic bacteria. Arab J Chem 13(12):9139–9144

    Article  CAS  Google Scholar 

  48. Ganash EA, Altuwirqi RM (2021) Size control of synthesized silver nanoparticles by simultaneous chemical reduction and laser fragmentation in Origanum majorana extract: antibacterial application. Materials (Basel) 14(9):2326. https://doi.org/10.3390/ma14092326

    Article  CAS  PubMed  ADS  Google Scholar 

  49. Elangovan K, Elumalai D, Anupriya S, Shenbhagaraman R, Kaleena PK, Murugesan K (2015) Phyto mediated biogenic synthesis of silver nanoparticles using leaf extract of Andrographis echioides and its bioefficacy on anticancer and antibacterial activities. J Photochem Photobiol, B 151:118–124. https://doi.org/10.1016/j.jphotobiol.2015.05.015

    Article  CAS  PubMed  Google Scholar 

  50. Priyadarshini JF, Sivakumari K, Selvaraj R, Ashok K, Jayaprakash P, Rajesh S (2018) Green synthesis of silver nanoparticles from propolis. Res J Life Sci Bioinform Pharm Chem Sci 4:23–36. https://doi.org/10.26479/2018.0404.02

    Article  CAS  Google Scholar 

  51. Muthu K, Priya S (2017) Green synthesis, characterization and catalytic activity of silver nanoparticles using Cassia auriculata flower extract separated fraction. Spectrochim Acta A Mol Biomol Spectrosc 179(15):66–72. https://doi.org/10.1016/j.saa.2017.02.024

    Article  CAS  PubMed  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Pathology, Jiroft Branch, Islamic Azad University, Jiroft, Iran

    Zeinab Fotoohiyan

  2. Department of Microbiology, Jiroft Branch, Islamic Azad University, Jiroft, Iran

    Mansour Karimdadi

  3. Horticultural and Crops Research Department Southern Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Jiroft, Iran

    Ali Salehi Sardoei

  4. Department of Agronomy and Plant Breeding, Islamic Azad University, Arak, Iran

    Fatemeh Bovand

  5. Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, 38156-8-8349, Iran

    Mansour Ghorbanpour

  6. Institute of Nanoscience and Nanotechnology, Arak University, Arak, 38156-8-8349, Iran

    Mansour Ghorbanpour

Authors
  1. Zeinab Fotoohiyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mansour Karimdadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ali Salehi Sardoei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fatemeh Bovand
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mansour Ghorbanpour
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

A.S.S. supervised the research. Z.F. and F.S. performed the experiment and collected the data. M.G., F.B., and M.K advised the research and revised the manuscript critically. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Ali Salehi Sardoei or Mansour Ghorbanpour.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fotoohiyan, Z., Karimdadi, M., Salehi Sardoei, A. et al. Green synthesis of silver nanoparticles using Artemisia persica extract and evaluation of their activity on onion gray rot fungus. Biomass Conv. Bioref. (2024). https://doi.org/10.1007/s13399-024-05377-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13399-024-05377-9

Keywords

Search

Navigation