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Characterization of a metagenome-derived thermostable xylanase from Tengchong hot spring

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Abstract

A novel xylanase gene (denominated xynDRTY1) was identified from Tengchong hot spring by a metagenomic approach. Its amino acid sequence was 73.43% identical to a hypothetical protein from Bryobacterales bacterium. The codon-optimized XynDRTY1 gene was synthesized and overexpressed in Escherichia coli. The XynDRTY1 was purified by using Ni–NTA affinity chromatography. It exhibited activity with natural glycosides, such as beechwood xylan (21.2 ± 3 U/mg) and oat spelt xylan (8.2 ± 0.3 U/mg). Its optimum pH was determined to be 6.0 and optimum temperature of 65 ℃, along with its stability over 140% and 110% relative enzyme activity after incubation at 60 ℃ for 20 min and 120 min, respectively. Based on these findings, we believe that XynDRTY1, as thermostable xylanase, may prove useful for biotechnological applications.

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Acknowledgements

We gratefully acknowledge Prof. Wen-Jun Li and his team from Sun Yat-sen University for their guidance on our metagenomic data compilation and analysis.

Funding

This research was supported by the National Natural Sciences Foundation of China Regional Program (Grant Nos. 31660015 and 31860243) and Yunnan Applied Basic Research Projects (Grant Nos. 202101AU070138, 2017FB024, and 2017FH001-032).

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  1. Yi-Rui Yin and Lei Li contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture and Biological Science, Dali University, Dali, 671003, People’s Republic of China

    Yi-Rui Yin, Lei Li, Run-Feng Yang, Xin-Wei Li, Hong-Yan Liu, Peng Sang & Li-Quan Yang

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  1. Yi-Rui Yin
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  2. Lei Li
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Contributions

YRY, PS, and LQY conceived the study. YRY and WH cloned the gene and cultured strains. RFY and HYL purified the recombinant protein. LL measured enzymatic activity. XWL and ZLL performed the data analysis and mapping. YRY, WH, PS, and LQY wrote the manuscript. All authors discussed the results and commented on the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yi-Rui Yin or Li-Quan Yang.

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Yin, YR., Li, L., Yang, RF. et al. Characterization of a metagenome-derived thermostable xylanase from Tengchong hot spring. Biomass Conv. Bioref. 14, 10027–10034 (2024). https://doi.org/10.1007/s13399-022-03296-1

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