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Viral genome silencing by neuronal sirtuin 1

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Abstract

Neurotropic viruses remain dormant in sensory neurons for years, but upon reactivation, they can produce multiple disease states including pain symptoms. Latent viral DNA is extrachromosomal, maintained as a circular episome bound to histones. Here, we show the regulation of an adenoviral genome by the nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylator Sirt1 in dorsal root ganglion neurons. Pharmacological modulation of Sirt1 and Sirt1 overexpression both affected viral transgene expression. We propose that age or stress-related neuronal NAD+ depletion may be a trigger for viral reactivation.

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Acknowledgements

The adenovirus encoding mouse Sirt1 was a kind gift from M. Zhang, Boston University. We thank L. Christen and W. Ruyechan for help with adenovirus. We thank W. Sirgurdson for help with microscopy. This study was supported by a John R. Oishei Foundation grant to A.B.

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Authors and Affiliations

  1. Program in Neuroscience, The State University of New York at Buffalo, 102 Farber Hall, 3435 Main Street, Buffalo, NY, 14214, USA

    Kelly E. Picchione & Arin Bhattacharjee

  2. Department of Pharmacology and Toxicology, The State University of New York at Buffalo, 102 Farber Hall, 3435 Main Street, Buffalo, NY, 14214, USA

    Arin Bhattacharjee

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  1. Kelly E. Picchione
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  2. Arin Bhattacharjee
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Correspondence to Arin Bhattacharjee.

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Picchione, K.E., Bhattacharjee, A. Viral genome silencing by neuronal sirtuin 1. J. Neurovirol. 17, 184–188 (2011). https://doi.org/10.1007/s13365-010-0012-3

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  • DOI: https://doi.org/10.1007/s13365-010-0012-3

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