Rescue of Methyl-CpG Binding Protein 2 Dysfunction-induced Defects in Newborn Neurons by Pentobarbital

Rett syndrome is a neurodevelopmental disorder that usually arises from mutations or deletions in methyl-CpG binding protein 2 (MeCP2), a transcriptional regulator that affects neuronal development and maturation without causing cell loss. Here, we show that silencing of MeCP2 decreased neurite arborization and synaptogenesis in cultured hippocampal neurons from rat fetal brains. These structural defects were associated with alterations in synaptic transmission and neural network activity. Similar retardation of dendritic growth was also observed in MeCP2-deficient newborn granule cells in the dentate gyrus of adult mouse brains in vivo, demonstrating direct and cell-autonomous effects on individual neurons. These defects, caused by MeCP2 deficiency, were reversed by treatment with the US Food and Drug Administration-approved drug, pentobarbital, in vitro and in vivo, possibly caused by modulation of γ-aminobutyric acid signaling. The results indicate that drugs modulating γ-aminobutyric acid signaling are potential therapeutics for Rett syndrome. Electronic supplementary material The online version of this article (doi:10.1007/s13311-015-0343-0) contains supplementary material, which is available to authorized users.


Introduction
Rett syndrome (RTT) is an X-linked dominant progressive neurodevelopmental disorder that primarily affects females and is sporadic in most cases, with a worldwide incidence of 1 in 10,000-15,000 female births [1]. Girls born with this syndrome develop normally until 6-18 months of age and then fail to achieve, or regress from, the expected developmental growth milestones. They also lose motor skills and speech, and display stereotypic hand-wringing motions. Most patients also develop other symptoms such as breathing abnormalities, hypoactivity, ataxia, seizures, and scoliosis, followed by cognitive deterioration [2]. Spontaneous mutations in the gene encoding the methyl-CpG-binding protein 2 (MeCP2) located at Xq28, have been identified as the underlying cause of most cases of RTT [1]. As the name suggests, MeCP2 binds preferentially to DNA on methylated CpG and recruits other co-repressors, such as SIN3 transcription regulator family member A (Sin3A) histone deacetylases or nuclear receptor co-repressor 2/SMRT co-repressor complexes, to influence transcription [3,4]. MeCP2 is responsible for stable and reversible repression of downstream methylated targets, although emerging evidence suggests that MeCP2 may also switch on certain genes and regulate RNA splicing [5]. Thus, a loss of MeCP2 would lead to inappropriate gene expression.
As the phenotypes of RTT manifest themselves over specific stages and develop gradually during childhood, MeCP2 deficiency has been most prominently linked to deficiencies in postnatal neuronal development. However, a study demonstrating effects of knocking out MeCP2 in adult animals indicates that MeCP2 is also required for adult neural function [6]. The progression and pathology of RTT and MeCP2-related disorders are still poorly understood. Phenotypic reversal of MeCP2 deficiency was achieved by restoring MeCP2 function in a RTT syndrome mouse model (MeCP2 knockout) [7], as well by systemic administration of an adeno-associated virus bearing MeCP2 cDNA [8], suggesting that RTT may be potentially treatable in humans. However, gene therapy for neurologic ailments in human patients has been difficult to achieve. Therefore, it is desirable to identify drugs that can overcome the defects associated with RTT. Aminoglycoside antibiotics, such as gentamicin, have been shown to cause a partial read-through of nonsense mutations within MECP2 [9][10][11][12]. Gentamicin has also been shown to increase MeCP2 protein levels and synapse numbers in neurons differentiated from induced pluripotent stem cells derived from patients with RTT [13]. Growth factors such as insulin-like growth factor-1 can also partially reverse RTT-like symptoms and phenotypes in MeCP2 mutant mice and neurons differentiated from RTT induced pluripotent stem cells [13,14], while acute benzodiazepine treatment can transiently abolish the breathing defects of MeCP2-deficient mice and alter expression of brainderived neurotrophic factor in mouse hippocampus [15,16]. However, none of these approaches causes complete recovery of normal function, so that identification of better therapeutic agents for RTT is necessary.
It has been shown that MeCP2-related disorders display neurologic phenotypes that can be attributed, in part, to postnatal hippocampal dysfunction [17][18][19]. We have therefore examined the cell-autonomous actions and functional role of MeCP2 in the development and maturation of newborn cells from the hippocampus of fetal brains, and also in newborn cells in the adult hippocampus in vivo. MeCP2 deficiency resulted in changes in dendritic structure, reduced synapse number and impaired synaptic transmission and reduced neuronal network activity. We also demonstrate that pentobarbital (PB) can reverse the structural and synaptic phenotypes resulting from MeCP2 deficiency, revealing the potential of γ-aminobutyric acid (GABA) A receptor (GABA A R) modulators such as PB as novel therapeutic treatments for RTT in the future

Construction, Production, and Stereotaxic Injection of Engineered Retroviruses
High titers of engineered self-inactivating murine retroviruses or lentiviruses (1 × 10 9 unit/ml) were produced as previously described [20,21]. Two short hairpin RNAs (shRNAs) targeting different regions of mouse MeCP2 and one control shRNA with a scrambled sequence were designed and cloned into retroviral or lentiviral vectors: GGGAAACTTGTTGT CAAGATGCC (sh834 or shMeCP2); GGAGTCTTCCAT ACGGTCT (sh1049); AGTTCCAGTACGGCTCCAA (shctrl). A shRNA with a mutated sh834 (GCGAAACTC-GTTGTTAAGATGGC) sequence was generated (shMut) to further validate the specific effects of MeCP2 knockdown in mice. GGGAAACTTCTCGTCAAGA (shMeCP2 against rat) was cloned into a lentiviral construct for knocking down MeCP2 in rat primary hippocampal neurons. Lentiviral expression constructs of mouse MeCP2-internal ribosomal entry site-enhanced green fluorescent protein were cotransfected with retroviral constructs carrying shctrl, sh834, shMut (shRNA with mutated sh834), or sh1049 into human embryonic kidney (HEK) 293 cells to validate the specificity and efficiency of the shRNAs using Western blot analysis. Lentivirus carrying either shctrl or sh834 (henceforth termed BshMeCP2^) were produced to infect postmitotic primary hippocampal neurons to confirm the specificity and efficiency of the shRNAs in these neurons using Western blot analysis [at 14 days in vitro (DIV)] and immunofluorescence (at 5 DIV).
For in vivo studies, adult (7-8 weeks old) female C57Bl/6 mice were anesthetized and different sets of retroviruses were stereotaxically injected into the dentate gyrus at 4 sites. The mice were euthanized at 14 days after viral injection for morphological analysis, as previously described [20,21]. All animal procedures and applicable regulations of animal welfare were in accordance with institutional animal care and use committee guidelines. For pharmacologic modulation in vivo, mice received intraperitoneal injections of PB (1 mg in 100 μl saline per mice) every 2 days after virus injection, until day 14, when they were euthanized for analysis.

Hippocampal Neuron Primary Cultures
Primary cultures of hippocampal neurons were isolated from embryonic day 18 (E18) Long-Evans rats as described previously [21][22][23]. Briefly, the hippocampus was carefully extracted from the brain and collected in buffer (127 mM NaCl, 5 mM KCl, 170 μM Na 2 HPO4, 205 μM KH 2 PO4, 5 mM glucose, 59 mM sucrose, 100 U/ml penicillin/streptomycin, pH 7.4). Cells were dissociated, washed, and collected in growth medium [Dulbecco's Modified Eagle's Medium w/ GlutaMax (Invitrogen, Carlsbad, CA, USA) containing 1 M 4-(-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 10% heat inactivated horse serum (Invitrogen), and 100 U/ml penicillin/streptomycin (pH 7.4)]. Cells were plated at 1.5 × 10 6 cells per 24-well plate for all in vitro experiments, including morphological analysis, calcium imaging, and electrophysiological recordings. Cytosine arabinoside (10 μM) was added to the neuronal culture to eliminate dividing cells (astrocytes and microglia). Neuronal cultures were infected with a lentiviral vector carrying shctrl or shMeCP2, and cultured for 12-14 days. For chronic drug treatment, neurons were infected with virus and then grown for another 24 h before being treated with vehicle, 5 μM PB, 5 μM bicuculline, or 25 μM musimol. Culture media were replaced with fresh media with drugs every other day throughout the entire culture period. At least 5-6 batches of culture were used for each experiment. Each batch of cultures was isolated from pooled hippocampus of all E18 pups (typically 8-10) from 1 animal.
Because the cultures consisted of both excitatory and inhibitory neurons, we took the following measures to ensure that differential contributions from neuronal subtypes did not bias the data: 1) neurons were randomly selected; 2) data were recorded from a large number of neurons (in all cases at least 10 cells, as indicated in the figure legends); 3) blind experiments were performed so that the person doing the recordings did not know which experimental group the neurons came from.

Calcium Imaging
Hippocampal primary neurons were washed twice with loading buffer containing the following: 118 mM NaCl, 4.69 mM KCl, 4.2 mM NaHCO 3 , 1.18 mM KH 2 PO, 0.8 mM MgCl 2 , 2.0 mM CaCl 2 , 20 mM HEPES, and 30 mM glucose (pH 7.4), and incubated with final concentration of 1 μM X-rhod-1 (Molecular Probes/Invitrogen, Carlsbad, CA, USA) for 30 min at 37°C. Excess dye was removed by washing twice with loading buffer, and an additional 20-min incubation was done to equilibrate the intracellular dye concentration and allow for de-esterification. Time-lapse image sequences of 500 frames were acquired with a sampling rate of 0.64 Hz with a region of 512 × 512 pixels, with 488-nm (fluorescein isothiocyanate) and 534-nm filters on a LSM 710 inverted fluorescence confocal microscope (Carl Zeiss, Singapore). Images were acquired with ZEN software (Carl Zeiss) and data analysis was done using MATLAB (Mathworks, Natick, MA, USA). At least 20 green fluorescent protein-positive neurons were randomly selected for calcium imaging (as indicated in the figure legends) from at least 3 coverslips/batch.
For each experiment, 20 regions of interests were selected to record the calcium intensity of 20 cells under fluorescence, with a sampling rate of 1.56 s/frame. The typical firing rate of these cells in these experiments is 2-20 spikes/min [24], and the duration of each calcium spike is normally longer than 2 s; therefore, our sampling rate was high enough to capture spike events. Peak detection was done in MATLAB according to previous studies [13], with the criterion of ΔF/F > 10%, where F represents calcium intensity and ΔF is the change of intensity in consecutive frames. The amplitude of each peak is measured by the difference between the peak value and the baseline.
The correlation coefficient r xy (τ) for each pair of neurons was computed based on previously published equations [25]. A peak at the center, where τ = 0, means the 2 neurons are synchronized, while a shifted peak indicates a likely causal relationship with certain delay. For each culture, the average correlation coefficient of all possible pairs among the 20 selected neurons was calculated. Neurons that did not generate detectable spikes were excluded from the calculation. To measure the strength of synchronicity in each culture, a synchronicity index was obtained by the average difference between the peak in the correlation coefficient and the baseline value [26,27]. Therefore, a higher synchronicity index indicates stronger synchronization in activity. The cells that did not have detectable spikes were excluded when calculating average peak amplitudes of calcium spikes. For interspike interval statistics, only the cells that had > 2 spikes were taken into consideration.

Confocal Imaging and Analysis
Cells were fixed and processed for the immunocytochemistry procedure using antibodies against Synapsin-1 (1:500; Abcam, Cambridge, UK), together with 4',6-diaminodino-2phenylindole (1:5000). Images were acquired on a LSM 710 confocal microscope or LSM 7 ELYRA PS.1 system (Carl Zeiss, Singapore). Coronal brain sections (40 μm) were prepared from virus-injected mice and processed as previously described [20,21]. The projected 3-dimensional reconstruction images were semiautomatically traced, and total dendritic length and branch number of each individual GFP-positive neuron in the granule cell layer were analyzed. The distribution patterns of dendritic arborization of each individual neuron under different conditions are shown in accumulative distribution plots. Statistical significance (p < 0.01) was assessed using the Kolmogorov-Smirnov test. Sholl analysis for dendritic complexity was carried out by counting the number of traced dendrites that cross a series of concentric circles at 5-μm intervals from the cell soma. Statistical significance (p < 0.05) was assessed using the Student's t test.

MeCP2 Deficiency Affects Neuronal Development
To examine the role of MeCP2 in neuronal development, we used shRNA to silence MeCP2 expression in HEK293 cells via retrovirus or in primary hippocampal neurons from rat fetal hippocampus by using lentivirus (Fig. 1a-d). The efficiency of knockdown was determined by Western blot analysis and immunocytochemistry ( Fig. 1b-d). We found that a specific MeCP2-directed shRNA (shMeCP2) was very effective in reducing MeCP2 levels, while a scrambled construct (shctrl) did not ( Fig. 1b-d). As a further control, we found that a point-mutated version of the active shRNA (shMut) was also ineffective in reducing MeCP2 levels (Fig. 1b).
Silencing MeCP2 in cultured neurons resulted in less elaborate outgrowth of neurites compared with cells infected with the scrambled control, shctrl (Fig. 1e,f). This was evident both as a reduction in the total length of dendrites and as a decrease in the number of dendrites (Fig. 1e,f). We also observed a significant reduction in the number of synapses per dendritic trunk (length in μm), as detected with immunostaining for synapsin-1 (Fig. 1g, h), a marker of presynaptic terminals.
Next, to examine the cell-autonomous effect of MeCP2 in vivo, we injected retrovirus carrying shRNA directed against MeCP2 in vivo into the dentate gyrus of adult mouse brains, allowing genetic manipulation of individual progenitor cells in the adult dentate gyrus. This resulted in less elaborate dendritic arborization than in control cells that received the control shRNA (Fig. 1i, j), indicating a direct and cellautonomous effect of MeCP2 on these cells. This was evident in measurements of the total length of dendritic processes and the number of dendritic branches (Fig. 1j, left and Fig. 1j, right, respectively). These results demonstrate that loss of MeCP2 has similar effects in dendrite development, both on cultured newborn hippocampal neurons in vitro from early development and on newborn neurons in vivo in developed adult brains.

MeCP2 Deficiency Affects Functional Properties of Neurons
To determine whether the dendrite defects caused by MeCP2 knockdown are translated into functional defects, we next examined the effects of MeCP2 knockdown on the functional properties of neurons. MeCP2 knockdown caused substantial changes in the intrinsic properties of cultured neurons. We found that the membrane capacitance (C m ) was smaller in MeCP2 knockdown neurons than in control cells (Table 1). Because C m is proportional to the plasma membrane surface area, this reduction in C m is presumably due to the shorter dendritic length of these cells (Fig. 1e,f). Likewise, the input resistance of MeCP2-deficient neurons was higher than that of control cells (Table 1). This is also consistent with the smaller plasma membrane area of these neurons because input resistance varies inversely with surface area. While the resting membrane potential of MeCP2 knockdown neurons was similar to that of control neurons, the number of action potentials evoked in response to depolarizing current pulses was higher (Fig. 2a,b). This presumably arises from the higher input resistance of these neurons, which creates larger voltage changes in response to a given amount of injected current. This can be seen in the current-voltage relationships shown in Fig. 2c; the slopes of these curves (the membrane resistance) were significantly different (t-stat = 9.149, p < 0.01) for control and MeCP2 knockdown neurons. A larger depolarization will generate more action potentials, as seen in Fig. 2a, b. There were no significant differences (p = 0.95) in action potential threshold or amplitude between control and knockdown neurons (Table 1). There was a modest (10%) reduction in spike duration, which might make a minor contribution to the increase in action potential firing. Thus, loss of MeCP2 does not significantly affect the intrinsic excitability of these cells but simply makes them smaller. This indicates that MeCP2 plays an indirect role in regulating neuronal excitability via changes in surface area.

MeCP2 Deficiency Affects Synaptic Transmission
To further examine the functional consequences caused by MeCP2 knockdown, we compared synaptic transmission in voltage clamped MeCP2 knockdown and control neurons. We first examined quantal release of the excitatory neurotransmitter, glutamate, by recording spontaneous mEPSCs (Fig. 2d). In these experiments, TTX was used to block action potential firing in presynaptic neurons, and the GABA receptor blocker bicuculline was also applied to eliminate inhibitory postsynaptic responses. The mean frequency of mEPSCs was markedly reduced in MeCP2-deficient neurons compared with control cells (Fig. 2e). Presumably, this is due to the decreased dendrite length and concomitant reduction in synaptic contacts observed in MeCP2 knockdown neurons ( Fig. 1e-h). In contrast, the amplitude of mEPSCs in MeCP2-deficient neurons was comparable with controls, evident both in the distribution of mEPSC amplitudes and in the mean amplitude of mEPSCs (Fig. 2f, g). This suggests that MeCP2 knockdown affects neither presynaptic packaging of glutamate into synaptic vesicles nor the properties of postsynaptic glutamate receptors.
To examine effects of MeCP2 knockdown on network synaptic activity, we recorded spontaneous postsynaptic currents in cultured hippocampal neurons in the absence of receptor blockers and without TTX (Fig. 3a). Two types of spontaneous EPSCs were observed: discrete, unitary EPSCs, and bursts of superimposed EPSCs that presumably represent synchronous excitation of the network of presynaptic neurons (see inset in Fig. 3a). Both types of EPSC were markedly reduced in MeCP2 knockdown neurons. Unitary EPSCs, including those resolvable during bursts, were present at a mean frequency of approximately 20 Hz in control neurons but their frequency was reduced by > 50% in MeCP2 knockdown cells (Fig. 3b). Analysis of the amplitudes of spontaneous EPSCs revealed a selective loss of relatively large events in response to MeCP2 knockdown (Fig. 3c), leading to a reduction in mean EPSC amplitude (Fig. 3d). Likewise, there was a reduced frequency of bursts of EPSCs in MeCP2 knockdown neurons (Fig. 3e). Analysis of peak EPSCs amplitude revealed 2 main populations of events: 1 group in the range of 10-100  pA, reflecting unitary events, and a larger group, in the range of 100-1000 pA, reflecting EPSCs in the bursts (Fig. 3f). While there were fewer events in both EPSC populations in MeCP2 KD neurons, there was a preferential reduction in the largest EPSCs within bursts (Fig. 3f). As a result, the maximum amplitude of EPSC bursts was reduced in MeCP2 knockdown neurons (Fig. 3g).
In control conditions, the spontaneous bursts of excitatory synaptic events could be observed to elicit action potentials (Fig. 3h, upper trace). Consistent with the reduction in the frequency of EPSC bursts in MeCP2 knockdown neurons (Fig. 3e), spontaneous action potentials evoked by spontaneous bursts of excitatory synaptic events were much rarer in these neurons (Fig. 3h, lower  trace). Quantification revealed that the frequency of these spontaneous action potentials was greatly reduced in the MeCP2 knockdown cells (Fig. 3i). Thus, the loss of MeCP2 translates to reduced excitation within synaptic networks, despite the fact that responsiveness to depolarizing currents is increased (Fig. 2b,c).

MeCP2 is Important for Neuronal Network Activity
We next looked at the effects of these network changes on the spatial organization of neuronal activity by using calcium imaging to measure the activity of many neurons simultaneously (Fig. 4). In these hippocampal neurons, neuronal activity is associated with spontaneous and synchronous rises in intracellular calcium concentration (calcium spikes) [28]. Here, we observed a decrease in the frequency of these calcium spikes in MeCP2deficient neurons compared with control neurons (Fig. 4a,b), presumably due to the lower rate of synaptically driven action potentials in these cells (Fig. 3h,i). Raster plots indicated that although calcium spike frequency was decreased in MeCP2-deficient neurons, these signals were much more synchronous across neurons (Fig. 4c). Cross-correlation analysis showed a higher degree of synchronization, evident as a higher peak at time = 0 (Fig. 4d). This was confirmed by calculating the synchronicity index (see BMaterials and Methods^), which was significantly higher in the MeCP2-deficient neurons (Fig. 4e). In addition, there was an increase in the amplitude of calcium spikes in the MeCP2-deficient neurons (Fig. 4a, f, g). Increased synchronicity and reduced spike frequency have been suggested to result from a decrease in the number of neurons firing independently [29]. Moreover, more neurons firing synchronously leads to larger calcium spikes [29].

PB Treatment Rescues Dendritic Defects and Synaptic Functions Associated with MeCP2 Dysfunction
MeCP2 plays a role in neuronal development and connectivity (Figs. 1, 2, 3 and 4), and GABA signaling has been implicated in both [20,[30][31][32]. Specifically, it has previously been demonstrated that conversion of GABA-induced depolarization into hyperpolarization promotes dendrite development of newborn neurons, and GABA regulates synaptic integration of these newly generated neurons in the adult brains [20]. Moreover, the level of GABA expression in the hippocampus of MeCP2-deficient mice at p55 (late disease stage) is significantly lower than in wild-type mice [30]. Therefore, we asked whether a drug that potentiates GABA signaling can promote   dendritic development of newborn neurons and thereby rescue the defects of MeCP2 dysfunction. We treated the cells with a US Food and Drug Administration-approved drug, PB, a positive allosteric modulator of GABA A R [33], used for the treatment of seizures and for preoperative sedation. Remarkably, compared with neurons treated with vehicle alone, chronic treatment of MeCP2-silenced neurons with PB significantly (p < 0.05) increased total neurite length (Fig. 5a), the number of dendritic branches (Fig. 5b), and the number of synapsin-1positive synapses (Fig. 5c). However, PB had no significant effect on control neurons expressing scrambled control shRNA (Fig. 5a-c).
To determine whether PB could also rescue the defects in synaptic function, mEPSCs were measured in MeCP2 knockdown neurons after chronic PB treatment.
In these experiments, the PB was removed immediately prior to starting the recordings in order to avoid any possible complications associated with acute effects of the PB on GABA receptors. In these conditions, the frequency of mEPSCs was significantly (p < 0.05) rescued by PB to values approaching those observed in control cells (Fig. 5d,e). This is consistent with the effect of PB treatment on synapse number (Fig. 5c). However, PB had no effect on mEPSC amplitude (Fig. 5d,f), which was unaffected by MeCP2 silencing. We also chronically treated the cells with an antagonist (bicuculline) and an agonist (muscimol) of GABA A R (Fig. 5g,h). Muscimol partially rescued the frequency of mEPSCs, while bicculline did not (Fig. 5g). This suggests that at least some of the effects of PB are due to the actions of this drug on GABA A R, though it is also likely that PB has additional targets.

PB Treatment Rescues Network Activity in MeCP2-deficient Neurons
We next asked whether PB could reverse the effects of loss of MeCP2 on network activity. We found that PB increased the frequency of calcium spikes (Fig. 6a), and reversed the synchronizing effect of MeCP2 KD (Fig. 6b,c), although both of these effects were also observed in cells with the control shRNA (Fig. 6a-c). PB did not have any effect on the amplitude of spontaneous calcium spikes in control cells but significantly decreased the amplitude of these calcium spikes in MeCP2-deficient cells (Fig. 6d). Chronic treatment with bicuculline reduced the frequency of calcium spikes, while treatment with muscimol or PB had the opposite effect (Fig. 6e). Interestingly, bicuculline and PB, but not muscimol, reversed the increase in calcium spike amplitude observed in MeCP2 knockdown neurons (Fig. 6f). Thus, all the data are consistent with the conclusion that the functional properties of GABAergic synapses are enhanced in MeCP2-deficient cells and rescued by PB treatment. As GABA regulates synaptic integration of newly generated neurons in the adult brains [20], we next determined whether PB, a modulator of GABA signaling, can rescue the structural defects caused by MeCP2 dysfunction in vivo. We analyzed neuronal morphologies after chronic PB treatment, employing the experimental design illustrated in Fig. 7a. Such chronic PB treatment increased total dendrite length and total number of branches (Fig. 7b-e). These findings suggest that PB can also compensate for the defects caused by MeCP2 dysfunction in newborn neurons in vivo. However, PB also enhanced dendrite outgrowth in control neurons (Fig. 7b,c). Interestingly, this morphological effect of PB on control neurons was not observed in cultured hippocampal neurons in vitro (Fig. 5a-c).
Taken together, our data indicate that MeCP2 knockdown causes both structural and electrophysiological phenotypes in fetal hippocampal neurons in culture. MeCP2 knockdown also causes similar, cell-autonomous defects in dendrite development in newborn neurons in the adult brain. These phenotypes can be reversed, at least in part, by chronic PB treatment both in vitro and in vivo, possibly through modulation of GABA signaling.

Experimental Strategy
MeCP2 has been found to be important for dendritic branching and spine density in various types of cells, including motor cortical neurons [34], cortical pyramidal neurons [35], and hippocampal pyramidal neurons [36][37][38]. However, the effects on neuronal morphology vary according to age, cell type, and the particular MeCP2 mutation considered [39]. Our study focused on the effects of MeCP2 knockdown in a single brain region, the hippocampus, where MeCP2 deficiency is known to affect hippocampal functions such as learning and memory [18]. This allowed us to study the effects of MeCP2 deficiency in a well-defined population of neurons both in vitro and in vivo.
In the hippocampus of a MeCP2 knockout mouse created by deleting exon 3, containing the methyl CpG binding domain of Mecp2, early neurogenesis is normal but maturation is impaired, leading to defects in presynaptic protein expression and the distribution of dendritic spines [40]. This could be due to direct effects upon hippocampal neurons. However, nonneuronal cells such as glia and microglia have also been shown to play a major role in regulating dendritic morphology and synapses [41][42][43][44]. Consequently, knockout mice in which MeCP2 has been deleted from all cell types cannot distinguish between cell-autonomous and non cell-autonomous (or secondary) effects of MeCP2 in neurons. We were able to address this issue by using a retrovirus strategy to manipulate genetically individual progenitor cells in normal adult mice (Figs. 1 and 7). Our strategy took advantage of the fact that the hippocampal dentate gyrus region is one of the few brain areas in which there is continuous neurogenesis in adults. Therefore, it is an excellent system to study cell-autonomous effects of MeCP2 in individual neurons using retrovirus-mediated gene knockdown. This allowed us to circumvent the limitations associated with all currently available MeCP2 knockout mouse models of RTT. The fact that we observed dendritic abnormalities in MeCP2-deficient dentate gyrus granule newborn neurons in the hippocampus of normal adult brain indicates a cell-autonomous effect of MeCP2 deficiency in these neurons.

Effects of MeCP2 Deficiency
We have observed that knocking down MeCP2 in cultured hippocampal neurons has several effects: it shortens dendrites,  increases responses to depolarizing current pulses, reduces the number of synapses, reduces the frequency of spontaneous synaptic events, reduces the frequency of spontaneous action potentials, and reduces the number of spontaneous calcium spikes, a measure of spontaneous neuronal firing. We propose that all of these arise from a common source, namely the control of dendrite growth by MeCP2. For a given depolarizing stimulus, shMeCP2 neurons fired action potentials at a higher frequency than control neurons (Fig. 2b). This presumably arises from the higher input resistance of these neurons, which creates a larger voltage response for a given amount of injected current, as observed in Fig. 2c. A larger depolarization will generate more action potentials, leading to the observed increased rate of action potential firing shown in Fig. 2b. The higher input resistance is consistent with, and presumably is due to, the smaller surface area of these cells (Fig. 1g). The smaller surface area can also explain the increased membrane capacitance of these cells. There were no significant differences in action potential threshold or amplitude between control and MeCP2-deficient cells. Thus, loss of MeCP2 does not significantly affect the intrinsic excitability of these cells but instead simply changes their passive electrical properties by making them smaller. There was a small (10%) reduction in spike duration, which might contribute modestly to the increased action potential firing.
The fewer synapses observed in the MeCP2 knockdown neurons is likely to be due to the less elaborate dendrites of these cells. Fewer synapses would reduce the frequency of spontaneous excitatory synaptic input, both mEPSCs and network-driven excitatory input, as observed (Figs 2e, and 3b, e). This reduction in excitatory drive should reduce spontaneous excitation of these neurons, precisely as observed (Fig. 3h, i). Further, this should decrease recurrent excitation of all neurons in the network, yielding the observed decrease in the rate of calcium spike activity.
The observed loss of synapses is in agreement with other observations demonstrating that MeCP2 regulates synaptogenesis [31,45]. MeCP2-dependent transcriptional repression can also regulate excitatory neurotransmission in cultured hippocampal neurons. This includes studies showing that key proteins in the glutamate pathway are spatially and developmentally affected in Mecp2-deficient mice [30].
Spontaneous calcium spikes play a role in neural development and plasticity [13,28], and our results showed neuronal development is affected upon knocking down MeCP2. Previous studies showed that signaling via calcium plays a substantial role in the function of MeCP2, both in regulating gene transcription and in neuronal morphology and function [36,46]. MeCP2 has also been associated with calcium signaling, including disturbance of calcium homeostasis during early postnatal development in MeCP2 knockout mice [46]. RTT patient-derived neurons also showed altered calcium transients generated by synaptic activity [13]. The increase in the amplitude of calcium spikes in the MeCP2-deficient neurons may result from increased synchrony [29]. Similar effects of increased amplitude and decreased frequency are also seen in cortical neurons acutely treated with the GABA antagonist bicuculline [24].

PB Rescues the Effects of MeCP2 Deficiency
The noncompetitive GABA A R antagonist, picrotoxin, ameliorates behavioral and synaptic impairments associated with overexpression of MeCP2 [47]. We therefore determined whether modulating GABA signaling could reverse the structural and electrophysiological phenotypes resulting from MeCP2 deficiency. We found that chronic PB treatment can reverse the morphological and functional defects caused by silencing MeCP2 in cultured primary hippocampal neurons, as well as the morphological effects observed in newly generated granule cells in vivo. The synaptic effects of MeCP2 loss also could be partially reversed by muscimol, a GABA receptor agonist. This suggests the involvement of GABA receptor signaling in the effects of PB.
PB is a barbiturate that binds to GABA A R and potentiates inhibition of GABA A -type receptors [33,48]. Depending on its concentration, PB can potentiate (approximately 10-100 μM), activate (approximately 100-800 μM), or block (approximately 1-10 mM) the GABA A R channel [49]. The mechanisms underlying these actions remain poorly understood. Moreover, these studies were done with acute PB treatment, and the effects may differ from those caused by chronic treatment, where processes such as adaptation and tolerance of neurons may also play important roles [50]. There are only a few studies on chronic PB treatment, and these have mainly investigated its effects on expression of GABA receptor subunits. Collectively, these studies show that long-term PB treatment causes region-specific changes in the expression of GABA receptor subunits, with expression usually slightly downregulated or not significantly changed [50]. There is also little change in the maximum number of binding sites for GABA agonists such as muscimol [50]. Nonetheless, PB also has other targets in the brains, and exerts diverse effects on synaptic function apart from potentiating GABAergic transmission [48]. A better understanding of which specific components in GABA signaling is regulated and influenced by MeCP2 will be important for finding a more appropriate drug or compound that can be use as treatment for RTT. It would also be of interest to study the effects of chronic PB treatment in other brain regions in the future, in part to determine whether PB treatment could ameliorate such effects on GABA signaling.
Our data demonstrate a role for MeCP2 in growth and maturation of newborn granule cells in developing fetal and adult hippocampus, likely through GABA signaling. However, it is not clear whether MeCP2 is acting by repressing genes involved in GABA signaling or has other roles that have yet to be identified. Our results suggest that PB and related drugs that modulate GABA signaling are potential therapeutic candidates for treating RTT.