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FGD5-AS1 promotes growth and mobility of trophoblast cells by regulating IGF2 via sponging miR-16-5p in pre-eclampsia

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Abstract

Background

PE is a common pregnancy disease that can cause various pathologies in pregnant women. GEO microarray data revealed low expression of FGD5-AS1 in placenta tissues from patients with severe PE. As predicted by ENCORI, FGD5-AS1 sponges miR-16-5p, and IGF2, and promotes cell proliferation, differentiation, and metabolism.

Objectives

This study aimed to interrogate the interaction of FGD5-AS1/miR-16-5p/IGF2 axis and the mechanism of FGD5-AS1 in hypoxia-induced trophoblast cell injury.

Results

Our findings revealed that FGD5-AS1 and IGF2 were substantially decreased in PE patients than that in normal pregnant females, whereas miR-16-5p was increased in PE patients than that in normal pregnant females. In vitro studies showed that FGD5-AS1 were reduced in trophoblast cells after hypoxia treatment, accompanied by decreased viability and increased apoptosis, also reduced invasion, migration and angiogenesis. However, FGD5-AS1 overexpression substantially reversed these effects. Further mechanistic analysis showed that FGD5-AS1 could target miR-16-5p to regulate IGF2. FGD5-AS1 promoted the expression of IGF2 by sponging miR-16-5p, thereby increasing the viability of trophoblast cells after hypoxia treatment and promoting invasion and migration.

Conclusion

Overall, our findings demonstrated that FGD5-AS1 promoted growth and mobility of trophoblast cells by regulating miR-16-5p/IGF2 axis in PE.

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Data availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

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Funding

This work was supported by the Key Science and Technology Research Program of Jinhua City, Zhejiang Province (Grant No. 2021-3-120).

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Authors and Affiliations

  1. Department of Obstetrics, Jinhua People’s Hospital, No. 267, Danxi East Road, Jinhua, 321000, Zhejiang, China

    Limei Quan, Yan Jin, Jianfang Wang, Xiwang Dai & Shuli Zhu

  2. Department of Medical Laboratory, Jinhua People’s Hospital, Jinhua, 321000, Zhejiang, China

    Zengwei Sheng

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  1. Limei Quan
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  2. Yan Jin
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  3. Jianfang Wang
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  4. Xiwang Dai
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  5. Shuli Zhu
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  6. Zengwei Sheng
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Contributions

LQ and YJ designed the study, supervised the data collection, JW analyzed the data and interpreted the data, XD, SZ, and ZS prepare the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Limei Quan.

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Conflict of interest

A Limei Quan declares that he/she has no conflict of interest; Yan Jin declares that he/she has no conflict of interest; Jianfang Wang declares that he/she has no conflict of interest; Xiwang Dai declares that he/she has no conflict of interest; Shuli Zhu declares that he/she has no conflict of interest; Zengwei Sheng declares that he/she has no conflict of interest.

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Ethical approval was obtained from the Ethics Committee of Jinhua People’s Hospital (IRB-2020042-R).

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Quan, L., Jin, Y., Wang, J. et al. FGD5-AS1 promotes growth and mobility of trophoblast cells by regulating IGF2 via sponging miR-16-5p in pre-eclampsia. Mol. Cell. Toxicol. 20, 409–419 (2024). https://doi.org/10.1007/s13273-023-00357-y

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