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Pasteurella multocida specific bacteriophage suppresses P. multocida-induced inflammation: identification of genes related to bacteriophage signaling by Pasteurella multocida-infected swine nasal turbinate cells

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Abstract

Background

Although Pasteurella multocida is highly prevalent pathogen in animals and plays an important role in swine respiratory diseases, only a few studies on the use of bacteriophages specific to Pasteurella multocida disease have been reported.

Objective

The object of this study was to investigate the therapeutic effect of specific P. multocida bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells.

Methods

Pas-MUP-1 phages were applied 24 h prior to P. multocida infection (1 × 107 cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on P. multocida and Pas-MUP-1 treatment, we performed RNA sequencing experiments.

Results

We found that P. multocida-infected PT-K75 cells show increased gene expression of IL-1β, IL-6, and Muc1 in a dose-dependent manner. Interestingly, these genes resulted in decreased expression in P. multocida pretreated with the P. multocida-specific Pas-MUP-1 bacteriophage. RNA sequencing analysis revealed that bacteriophage administration regulated genes associated with immune and inflammatory responses, and the regulated genes were dramatically concentrated in the cytokine/chemokine-based signaling pathways. Pas-MUP-1 treatment was shown to regulate P. multocida induced gene expression in the bacteria.

Conclusion

These results suggest the specific bacteriophage has therapeutic potential as an alternative to antibiotic treatment to defend against P. multocida infection by altering inflammatory gene expression profiles.

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Acknowledgements

This work was supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through Technology Commercialization Support Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (817029-03-2-SB010).

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Authors and Affiliations

  1. Department of Cell Biology, Kosin University College of Medicine, 34 Amnam-dong, Seo-gu, Busan, 49267, Korea

    Ga Young Park & Kyoung Seob Song

  2. Institute of Life Technology, iNtRON Biotechnology, Seongnam, Korea

    Hyun Jin Yu & Jee Soo Son

  3. College of Veterinary Medicine, Kyungpook National University, Daegu, Korea

    Sang Joon Park

  4. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, Korea

    Hee-Jae Cha

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  1. Ga Young Park
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  2. Hyun Jin Yu
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  3. Jee Soo Son
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  4. Sang Joon Park
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  5. Hee-Jae Cha
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Contributions

GYP and KSS: Designed the study. GYP, HJY, and JSS: Performed experiments. JSS, SJP, HJC, and KSS: Analyzed data. GYP: Wrote the manuscript.

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Correspondence to Kyoung Seob Song.

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Park, G.Y., Yu, H.J., Son, J.S. et al. Pasteurella multocida specific bacteriophage suppresses P. multocida-induced inflammation: identification of genes related to bacteriophage signaling by Pasteurella multocida-infected swine nasal turbinate cells. Genes Genom 42, 235–243 (2020). https://doi.org/10.1007/s13258-019-00898-4

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  • DOI: https://doi.org/10.1007/s13258-019-00898-4

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