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Development and characterization of Morinda tinctoria incorporated electrospun PHBV fiber mat for wound healing application

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Abstract

As synthetic medications have various limitations and side effects in wound treatment, alternatively active wound dressing material could be designed by incorporating natural extracts. Electrospun fibers infused with plant extract can act as an active wound dressing material to speed up the process of wound healing. In this connection, the wound-healing efficacy of an Indian traditional drug Morinda tinctoria was reported earlier, but not yet explored as a natural active ingredient in electrospun fibers. The present work focused on preparing the extracts of M. tinctoria leaf and analyzing their yield, total phenolic concentration (TPC) and GC–MS phytochemical profile. The safety of methanol extract was studied in PBMC cells through MTT and live/dead assays and also TNF-α & IL-6 levels were quantified. Electrospinning of M. tinctoria-infused PHBV fiber mat was developed and characterized through SEM, FT-IR and TG–DTA. Results indicated that the methanol extract has a greater yield (2.47%) and TPC (4260.72 mg/L) when compared to other solvent extracts due to the presence of high polar molecules in M. tinctoria. GC–MS study revealed the phytochemicals such as coumarin and methylanthraquinone in the methanolic extract. MTT assay and live/dead staining revealed that the M. tinctoria extract was safe with 99% cell viability with intact cell wall. M. tinctoria induced the levels of TNF-α (328.75 pg/mL) and IL-6 (1357.14 pg/mL), which could improve the migration and proliferation of fibroblasts during wound healing. Development of M. tinctoria-infused fiber mat was optimized with 12% polymer concentration, 18 kV voltage, 0.01 mL/min flow rate and 14 cm tip to collector distance. SEM analysis of M. tinctoria-infused fiber mat revealed bead-less smooth fibers of 1.19 ± 0.15 µm diameter and 0.024 ± 0.003 µm pore size. FT-IR spectra revealed functional groups such as phenols, amines and alkanes in extract-incorporated fibers. A drop in temperature (457 °C) was noticed in M. tinctoria-infused fiber mat when compared to the control (595 °C), which indicates the degradation of organic matters in the fiber mat. M. tinctoria-infused fiber mat could be applied as wound dressing material after conducting suitable animal experiments.

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Acknowledgements

Authors are thankful to the Management of SASTRA Deemed University, Thanjavur, Tamilnadu for their encouragement and support.

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  1. Chemical Biology Lab (ASK-II-409), School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India

    Joshua Sharon Nissi, Srinivasakumar Vyaishnavi, Ragu Sivaranjanee & Vellingiri Vadivel

  2. Tissue Engineering and Additive Manufacturing (TEAM) Lab, Centre for Nanotechnology and Advanced Biomaterials (CeNTAB), School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, Tamil Nadu, India

    Muthu Parkkavi Sekar & Dhakshinamoorthy Sundaramurthi

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JSN, SV, RS and MPS: performed the experiments, collected data, interpreted the results & wrote the draft of the article; DS and VV: developed the concept, designed the work, supervised & made a critical revision of the article.

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Correspondence to Vellingiri Vadivel.

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The authors obtained permission from the Institutional Bio-safety Committee (IBSC Ref. No. SASTRA/IBSC/8/2021 Dated: 15/12/2021) for working with a human pathogen (MRSA). There are no animal / human studies reported in this paper.

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Nissi, J.S., Vyaishnavi, S., Sivaranjanee, R. et al. Development and characterization of Morinda tinctoria incorporated electrospun PHBV fiber mat for wound healing application. Macromol. Res. 31, 393–405 (2023). https://doi.org/10.1007/s13233-023-00149-2

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