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Enhanced Silk Fibroin-Based Film Scaffold Using Curcumin for Corneal Endothelial Cell Regeneration

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Abstract

The high demand for transplantable corneas is unlikely to subside anytime soon as there is a persistent shortage of cadaveric cornea. The goal of this study was to fabricate film scaffolds with desirable properties of a corneal endothelial cells (CECs) carrier. We used biocompatible materials (curcumin (CC) and silk fibroin (SF)) to construct transparent film scaffolds for CEC regeneration. The film scaffolds were subjected to surface analysis, transparency, stiffness, thermal characterization, and hydrophilicity evaluation. Biological activity of CECs on CC/SF film was analyzed by MTT assay, morphological analysis, mRNA expression, and histological study. Our results showed that the CC/SF film scaffolds had enhanced roughness and transparency compared to the pristine SF film scaffold. The hydrophilicity of the CC/SF film scaffolds showed a topographical environment that encouraged cellular interaction and tissue integration. All the films showed stable thermal characters and an improved capacity for cell growth when a proper amount of CC was incorporated into the SF film scaffolds. The results indicate that a robust scaffold is suitable for CEC transplantation.

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Author notes

  1. Equally-Contributed Authors.

Authors and Affiliations

  1. Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk, 54896, Korea

    Do Kyung Kim, Joo Hee Choi, Jeong Eun Song & Gilson Khang

  2. CHA Graduate School of Medicine, 120 Hyeryong-ro, Pocheon, Gyeonggi, 11160, Korea

    Do Kyung Kim, Sanghyuk Lee, Bo Sung Jung & Ki Soo Kim

  3. 3B’s Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Taipas, Guimarães, Portugal

    Rui L. Reis

  4. ICVS/3B’s - PT Government Associate Laboratory, Braga/Guimarães, Portugal

    Rui L. Reis

Authors
  1. Do Kyung Kim
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  2. Sanghyuk Lee
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  3. Joo Hee Choi
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  4. Bo Sung Jung
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  5. Ki Soo Kim
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  6. Jeong Eun Song
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  7. Rui L. Reis
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  8. Gilson Khang
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Contributions

Author contributions: D. K. Kim and S. Lee conceived and designed the study. J. H. Choi, B. S. Jung, K. S. Kim, and J. E. Song contributed to the mechanical characterization. R. L. Reis interpreted the results. D. K. Kim and S. Lee wrote the manuscript with input from all authors. G. Khang supervised the overall study and provided the reagents and materials.

Corresponding author

Correspondence to Gilson Khang.

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Acknowledgments: This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1I1A1A0107365711). This research was also supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2020R1A2C2103089).

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Kim, D.K., Lee, S., Choi, J.H. et al. Enhanced Silk Fibroin-Based Film Scaffold Using Curcumin for Corneal Endothelial Cell Regeneration. Macromol. Res. 29, 713–719 (2021). https://doi.org/10.1007/s13233-021-9081-9

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  • DOI: https://doi.org/10.1007/s13233-021-9081-9

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