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In vitro selection of RNA aptamer specific to Staphylococcus aureus

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Abstract

Staphylococcus aureus is a major foodborne pathogen. Gram-positive bacteria have unique teichoic acids as cell-wall components. In order to identify ligands specific to the bacteria, we developed an RNA aptamer against the teichoic acid of Staphylococcus aureus using SELEX technology. To this end, we used a polystyrene 96-well-based selection method and confirmed the binding activity of the RNA aptamer to the teichoic acid using real-time PCR. Of note, the teichoic acid-specific RNA aptamer was observed to bind to S. aureus bacterial cells also. This RNA aptamer could therefore be useful as a diagnostic ligand against S. aureus-associated foodborne illness.

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Acknowledgments

We thank Jin-Soo Maeng and Yong-Jin Cho (Korea Food Research Institute, Korea) for supplying the reagents and for useful discussions. We are grateful to Young Ju Lee and Won Il Lee (Dankook University, Korea) for their technical assistances.

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Authors and Affiliations

  1. Department of Integrated Molecular Sciences and Molecular Biology, Institute of Nanosensor and Biotechnology, Dankook University, 126, Jukjeon-dong, Suji-gu, Yongin, 448-701, Republic of Korea

    Seung Ryul Han & Seong-Wook Lee

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  1. Seung Ryul Han
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  2. Seong-Wook Lee
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Correspondence to Seong-Wook Lee.

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Han, S.R., Lee, SW. In vitro selection of RNA aptamer specific to Staphylococcus aureus . Ann Microbiol 64, 883–885 (2014). https://doi.org/10.1007/s13213-013-0720-z

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  • DOI: https://doi.org/10.1007/s13213-013-0720-z

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