Abstract
The present investigation was aimed to phytosynthesize silver nanoparticles (AgNPs) by utilizing aqueous extract of Mentha longifolia branches to reduce mass Ag into Ag° and evaluation of their potential to treat HCT116 colon cancer, Leishmanial, and bacterial cells. A characteristic Surface Plasmon Resonance (SPR) band to confirm synthesis was observed at ~ 450 nm of λ light by UV–Visible spectrophotometer. SEM and AFM micrographs unraveled that nanostructures are anisotropic, spherical and have a size under ~ 100 nm. Particle size analysis confirmed that the majority of the nanoparticles exist in ~ 6–20 nm of the size range. EDX investigation represented the highest peak of Ag with ~ 74% signal intensity. FTIR analysis confirmed the presence of the N–H (amine) group, C–N (cyanide) group and C≡C (alkynes) stretch as the surface capping agents. AgNPs were found biocompatible against human RBCs at a lower dose and LD100 was recorded as 117 μg/ml, which shows that a very high dose of AgNPs is required to cause toxicity to RBCs. The selective dose-dependent response of AgNPs was reported against Leishmania tropica and decreasing the dose of the nanoparticles resulted in increasing the survival rate of the pathogen (P < 0.05);10 μg/ml of AgNPs killed practically 67% of cells. Antibacterial action against plant bacterial pathogens was seen between 2 and 12 μg/ml. Annexin V apoptosis analysis and SRB examination showed that the plant aqueous extract and AgNPs are incapable to control the development of HCT116 colon cancer cells and only 3.77% cells indicated apoptosis. The results show the biocompatibility of phyto-functionalized nano-silver to selectively treat Leishmania and plant bacterial pathogens.
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Javed, B., Nadhman, A. & Mashwani, ZuR. Phytosynthesis of Ag nanoparticles from Mentha longifolia: their structural evaluation and therapeutic potential against HCT116 colon cancer, Leishmanial and bacterial cells. Appl Nanosci 10, 3503–3515 (2020). https://doi.org/10.1007/s13204-020-01428-5
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DOI: https://doi.org/10.1007/s13204-020-01428-5