Assessment of physico-chemical parameters and trace heavy metal elements from different sources of water in and around institutional campus of Lumami, Nagaland University, India

The main emphasis of this study was to assess the status of drinking water quality from four different water sources available in and around an institutional campus of Lumami, Nagaland University, with special reference to the detection of trace heavy metal elements. The results of the physico-chemical parameters of the water show that values of DO, BOD5 and free CO2 at S3 are beyond the permissible limit set by WHO and BIS. The values of DO in all the three stations (S1, S2 and S4) are near the maximum permissible limit of drinking water, indicating the deterioration of water quality, whereas the rests of the parameters are below the permissible limit of WHO and BIS. The detection of Hg in all stations along with alarmingly high concentrations of Fe and others such as Cu, Zn, Mn and Se is perceived as a matter of concern. On the other hand, As and Pb were not detected in any station. The detection of Hg and Fe in all the four types of water sources is an indication of contaminant hazards and needs drinking water treatment practices in these areas which in turn have implications on human health.


Introduction
Natural water scarcity combined with the lack of access to safe drinking water and inadequate sanitation services is common problem associated with developing countries including India (WHO 2006). Toxic water contaminants such as heavy metals and carcinogens remain of primary concern due to their ability to cause adverse health effects after prolonged periods of exposure (DEWO 1989). Water contaminants are primarily sourced from geological conditions, industrial and agricultural activities, and water pollutants are further categorized as microorganisms, inorganic, organic, radionuclides and disinfectants (Nollet 2000). The contamination of potentially toxic elements (PTEs) released from both natural (weathering and erosion of bedrocks and ore deposits) and anthropogenic (mining, agricultural and industrial) activities (Antoniadis et al. 2017a;Shah et al 2012) and finally contaminates the various water sources (Khan et al 2018;Tripathee et al 2016) that is nowadays a major environmental issue globally because of their highly toxicity behavior. PTEs such as iron (Fe), zinc (Zn), copper (Cu) and manganese (Mn) are essential elements and required for normal human body functions. However, the excess of it is dangerous to health if exceeded beyond their threshold limits (Ullah et al 2017). Rest of the PTEs like lead (Pb), cadmium (Cd), nickel (Ni), cobalt (Co), arsenic (As) and mercury (Hg) are extremely hazardous if present in trace concentration (Shakoor et al 2017;Saddique et al 2018).
Nagaland is located along the northeast corner of India extending between 25 0 6′ N to 27 0 4′ N latitude and 93 0 20′ E to 95 0 15′E longitude covering a total geographical area of 16,579 sq km. The state enjoys sub-tropical to warm temperate monsoonic types of climate with an annual mean rainfall of approximately 2600 mm. In rural regions, where wellmanaged water transportation system and related infrastructures are not available, spring, streams and well water serve as chief source of drinking water. The main water sources of water in Nagaland are rivers and streams, which depend heavily on the amount of annual rainfall. Lumami campus of Nagaland University is situated in the rural and backward region of Nagaland in Zunheboto district. Pollution in river by way of using explosive for fish hunting, rock quarrying, sand mining, shifting cultivation practices, alteration of freshwater flows, road construction, soil erosion in agriculture and hilly areas, deforestation and dam building is prevalent in the state which finally leads to deterioration of freshwater ecosystems. Peoples living in and around the campus of the University heavily depend on tap water which source is mainly from the river and streams. Spring and well water available in and around the institutional campus come in need during shortage supply of tap water during winter. No information is available about the scientific studies of drinking water quality and potential sources of contaminations for all these water sources. The main hypothesis of this study is to test the extent of pollution level in the water quality so as we can check whether the water is suitable for domestic purposes. The study area being in the headquarter of Nagaland University, is therefore highly crucial to evaluate the drinking water quality status from different sources of water bodies available in the area in order to assure safe drinking water of its residents. Hence, we envisaged the evaluation of drinking water quality from different sources like tap, spring, well and stream water available in and around the institutional campus of Nagaland University, Lumami, with an objectives of determining the various physico-chemical properties of water from different sources and to compare the values obtained with the permissible limit of drinking water quality given by Bureau of Indian standard (BIS 1991) and WHO (2004). Also the study aims to detect the contaminants of PTEs and other trace heavy metal elements in the water samples.
This study will help in identifying some of the important parameters of physico-chemical variables which shows good indices of water quality. Different sources of pollution and heavy metals analyzed from the study may help in tackling the problems of pollution by way of awareness to the public for stopping them from further creation of such types of pollution. Recommendation measures and management program can be taken up by the people residing in the area. The results obtained will help the public precautious about the water they use for domestic purpose by way of water treatment, thereby improving the quality of human health. Implementation of improved management strategies of these water sources can be taken up. Further, data generated from the study will be useful by media, institutions, academicians, water treatment plants, municipality and the policy decision makers.

Description of the sampling stations
Surface water samples for the analysis were collected from 4 sampling stations in and around the institutional campus of Nagaland University, Lumami, India. The four sampling stations selected were: Station 1 (S1): stream, station 2 (S2): tap water, station 3 (S3): well water and station 4 (S4): natural spring. The description and geographical coordinates of each sampling stations are described in Table 1. Location map of the sampling stations is shown in Fig. 1.

Analysis of physico-chemical parameters of water
From each sampling station, three water samples were collected monthly from September 2017 to April 2018 during morning hours of 9 a.m to 10 a.m in polyethylene plastic bottles rinsed three times with deionized water. Analysis could not be carried out for the month of January as the University was closed for winter vacation. Water temperature was measured on the spot using water thermometer. pH was measured with pen-type digital pH meter. DO was analyzed by Winkler's titrimetric method. BOD was estimated by Winkler's titrimetric method after 5-day incubation at 20 0 C followed by subtracting the values of titration from the initial and final DO. Free CO 2 was determined by titrating 100 ml of the water sample against 0.05 N NaOH solution and using phenolphthalein indicator. Estimation of chloride was done by argentometric method by titrating the samples against 0.2 N AgNO 3 solution using K 2 Cr 2 O 4 (potassium  chromate) as indicator. Total alkalinity was analyzed by titrating 100 ml of water samples against 0.02 N conc. H 2 SO 4 using methyl orange and phenolphthalein as indicator. Total hardness, calcium and magnesium hardness were determined titrimetrically by EDTA method using ammonia buffer solution and Eriochrome black-T as indicator. All the analysis were done in triplicates following standard methods prescribed by Trivedy et al. (1984); APHA (2005).

Trace Heavy metal elements analysis
Three water samples of each location were collected in polyethylene bottles that were pre-washed with laboratory detergent and rinsed with deionized water. The samples were treated with 1.5 ml of nitric acid and stored at 4 °C until analysis was done. The total metal concentration of arsenic, lead, cadmium, copper, iron, manganese, mercury, selenium and zinc in the filtered and digestive samples is determined in mg/l by using Atomic Absorption Spectrophotometer (AAnalyst 600 model, Perkin Elmer Inc., MA, USA). The AAS was calibrated with relevant grade standards.

Statistical analysis
The entire data set was computed for correlation matrix using SPSS (version 21.00). In order to evaluate the relationships between water quality parameters, Pearson's correlation matrix was executed for 11 parameters in 4 sampling station for three different seasons (summer, rainy and winter) with p < 0.5 and 0.01 level of significance.

Water temperature
Temperature, the measure of intensity of heat stored in a volume of water, is highly correlated with atmospheric temperature and the morphometric features. Distribution of plankton and the other organisms depend largely on surface water temperature (Chandrahas 2001) as it regulates various physico-chemical and biological activities (Raney et al. 1969). As observed from the present study, average surface water temperature from the four stations ranges from 16.01 to 18.85 °C (Table 2). Minimum temperature of 11 °C was observed during Dec 2017 at S4 and maximum of 23 °C in March 2018 at S2 (Fig. 2a). Seasonally, the values of temperature were highest during rainy season (20.1 °C) followed by summer (19 °C) and winter (15.33 °C) as shown in Fig. 2b. In general, atmospheric and water temperature depend on geographical location and meteorological conditions such as rainfall, humidity, cloud cover, wind velocity, incoming solar radiation, humidity, altitude. pH pH is a valuable parameter which guides not only the status of acid-alkali balance of the water but also serves as an important index of the degree of pollution. The factors like respiratory activity, exposure to air, temperature and pH 7.01 ± 0.08 7.26 ± 0.10 6.64 ± 0. 11 6.89 ± 0.14 6.64 ± 0.11-7.26 ± 0.10 6.5-8.5 6.5-8.  (Saxena 1987). Minimum pH of 4.7 was exhibited at S4 in April 2018 and a maximum of 7.8 at S2 in Nov 2017 (Fig. 3a). Seasonally, the values of pH were low during summer at S4 (6.39) and high during winter at S2 (7.60) as shown in Fig. 3b. In this study, the concentration of average pH ranges from 6.64 to 7.26 and all the water samples analyzed have concentration within the safe limit of 6.5 to 8.5 standard set by the BIS and WHO (Table 2). Low pH during summer may be due to the formation of carbonic acid with the release of more free carbon dioxide due to microbial respiration at high temperature and increase decomposition rate from the organic waste deposited.

Free CO 2
Free CO 2 is the normal component of all natural water. Biological oxidation of organic matter and dissolved CO 2 from air increases the level of CO 2 in water (Rajput et al. 2004). Free CO 2 in water is mainly originated from the respiration of aquatic biota, decomposition of organic matter and infiltration through the soil. Minimum free CO 2 of 11 mg/l was recorded at S1 during November 2017 and maximum of 103.4 mg/l at S3 during November 2017 (Fig. 4a). Average free CO 2 ranged from 19.17 to 62.70 mg/l. Seasonally the values of free CO 2 was lowest during rainy season at S2 (11 mg/l) and highest in winter at S3 (103.40 mg/l) as depicted in Fig. 4b. Free carbon dioxide is beyond the permissible limit given by WHO and BIS at S3 in well water (Table 3). Maximum value of free CO 2 at S3 in well water in winter may be due to microbial activity and respiration of micro-organisms. It imparts acidity to water because of the formation of carbonic acid. High levels of carbon dioxide in well water indicate the presence of dead material undergoing decomposition.

Dissolved Oxygen
Dissolved oxygen (DO) is one of the most important parameter in the study of aquatic environment in view of its important participation in many chemical and biological reactions, and it plays a vital role in supporting aquatic life and very much susceptible to environmental changes. It is of prime important factor in natural water both as regular of metabolic process of biotic community and indicator of aquatic health. Dissolved oxygen content of any water body depends on the mixing and aeration of water, water temperature, duration of sunlight received and altitude of the area (Lamare and Singh 2014 and  (Fig. 5a). Average DO lies within the range 2.93-3.99 mg/l. Seasonal values of DO was maximum during winter at S4 (7.63 mg/l) and minimum during summer at S3 (1.78 mg/l) as given in Fig. 5b. Maximum DO in winter may be because of low temperature and shorter day length. The anthropogenic activities also change DO due to addition of organic wastes, surface runoff and removal of vegetation, etc. Higher DO in well water during summer may be due to decrease in water level, stagnation of water body, high temperature accompanied with increased microbial activity due to presence of organic waste. DO at S3, i.e., well water, is below the permissible limit given by BIS and WHO (4-6 mg/l) as interpreted in Table 2. Low value of DO at S3 may be due to high temperature in summer accompanied with high rate of respiration of microbes followed by rapid decomposition of organic matter. Values of DO were low when compared with the work reported by Kibami et al (2014) from different ward of Mokokchung town in Nagaland where the value reported ranged from 7.00 to 14.30 mg/l.

Total alkalinity
The alkalinity of water is due to the presence of mineral salts, primarily carbonate and bicarbonate ions, present in it. Alkalinity makes water tasteful and helps in coagulation (Hussain 1987); nonetheless, it is preferable at less than 100 mg/l for domestic use. Lowest monthly variation in total alkalinity was found in September 2017 at S3 (15 mg/l) and also maximum in December 2017 at S3 (42 mg/l) as shown in Fig. 7a. Average total alkalinity lies within the range 18.32-30.28 mg/l (Table 2). Seasonally, the value of total alkalinity was found to be low during rainy season at S3 (15 mg/l) and high in winter (49.5 mg/l) at S2 (Fig. 7b). According to ISI the desired limit and the maximum permissible limit for alkalinity in drinking water are 200 and 600 mg/l, respectively (Table 2). Therefore, the values of total alkalinity content in all sampling stations have been found to be low as compared to desired limit value.

Chloride
Sources of chloride in natural water may be from soil, municipal or industrial sewage and wastes of animal origin (Girija et al. 2017) and often serve as a chemical pollution indicator for sewage contamination. Chloride content in potable water is given by WHO (1983) in 250 mg/l. Lowest chloride content was recorded at S4 (19.88 ± 0.02 mg/l) in November 2017 and highest at S3 (79.52 mg/l) in September 2017 (Fig. 8a). Average chloride values lie within the range 32.25-38.34 mgl/ (Table 2). Seasonally, chloride content was higher during rainy season at S3 (79.52 mg/l) and lower during winter (26.98 mg/l) at S4 (Fig. 8b). The high content of chloride during rainy season in well water (S3) may be due to storage of accumulated sewage during rainy season coupled with decaying process that accomplished by the microbes (Kumar et al 2005). Higher value of during rainy season in well water may be due to leached from rocks and water by weathering. The concentration of chloride was found within the permissible limit of BIS (250 mg/l), thus indicating the absence of chloride pollution sources. Values of Cl were within the range reported by Kibami et al (2014) from Mokokchung town (11.36-63.90 mg/l) except the value in S3; the value of Cl is greater than their values.

Electrical conductivity
It is a measure of capacity of a solution to carry an electrical current and is a total parameter for dissolved and dissociated substances which also indicates the concentration of dissolved electrolytes. Minimum conductivity value was reported at S3 (38.4 µS/cm) in September 2017 and maximum in March 2018 at S4 with a value of 272 µS/cm (Fig. 9a). Seasonally the value of conductivity was found to  Fig. 9b.
In the present study, the average values of electrical conductivity ranged from 91.41 to 115 µS/cm which is very low when compared with the standard limit for drinking water given by WHO (750 µS/cm) indicating good water quality (Table 2).

Total hardness
Total hardness is the parameter of water quality used to describe the effect of dissolved minerals (mostly Ca and Mg), determining suitability of water for domestic, industrial and drinking purposes. Minimum value of hardness was observed at S1 in April 2018 (28 mg/l) and maximum at S3 in March 2018 (370 mg/l) as depicted in Fig. 10a. Average total hardness ranges from 54.85 to 138.57 mg/l (Table 2). Seasonally, total hardness was maximum during summer at S3 (180.66 mg/l) and minimum during winter at S1 (42.66 mg/l) as shown in Fig. 10b. Higher values of total hardness at S3 (well water) during summer may be due to natural accumulation of salts and decrease in water volume due to less rainfall. Moshood (2008) opined that decreased hardness in winter may be due to more productivity in this season. The desirable limit of total hardness in potable water is 300 mg/l as given by Indian standards for drinking water (Table 2). Except S3 in well water which is near to the permissible limit, the remaining stations are safe for drinking

Calcium hardness as CaCO 3
Minimum value of Ca hardness as CaCO 3 was exhibited at S4 in April 2018 (10.5 mg/l) and maximum also in December at S3 (134.4 mg/l) as given in Fig. 11a.
Average values ranged from 21.90 to 64.80 mg/l. Seasonal value was highest during summer (161 mg/l) at S3 and lowest in winter (14 mg/l) at S1 as shown in Fig. 11b. High content of Ca hardness at S3 (well water) may be due to the application of lime during April 2017 as disinfectant and is beyond the permissible limit setup by BIS and WHO (75 mg/l) as illustrated in Table 2. Values of Ca hardness were comparatively higher than the value recorded by Kibami et al (2014) from Mokokchung town.

Magnesium hardness
Minimum value of Mg hardness was recorded at S3 (0.21 mg/l) in April 2018 and maximum in September 2017 at S3 (31.35 mg/l) as depicted in Fig. 12a. Average values ranged from 7.05 to 9.76 mg/l as shown in Table 3. Seasonal value was highest during rainy season (31.35 mg/l) at S3 and lowest during summer (3.88 mg/l) at S3 as given in Fig. 12b. Mg hardness obtained in the present study was within the range as obtained by Kibami et al (2014), i.e., 2.43 -15.10 mg/l except the value of S3 (31.35 mg/l) in well water is higher than their recorded values.

Correlation among the physico-chemical parameters of water
A good relationship between two variables is indicated by a correlation coefficient value near 1 or −1, whereas a value  Tables 3, 4 and 5 show the relationship between water quality parameters for three different seasons (summer, rainy and winter) using correlation matrix analysis. During rainy season, chlorine was found to be highly significant positively with DO (p < 0.05) and negatively with free CO 2 (p < 0.05), and Ca hardness shows significant negative correlation with free CO 2 (p < 0.05) and positively with alkalinity (p < 0.05). Conductivity was found to be highly significant negatively with free CO 2  (Table 3). Significant positive correlation between Ca hardness and alkalinity is due to the presence of carbonates, bicarbonates and hydroxides causing alkalinity in water (Sharma and Jain 2006). In summer, highly significant negative correlation was found between free CO 2 with BOD, Mg hardness with temperature at p < 0.05 level (Table 4). In winter, significant positive correlation at p < 0.05 level was observed for BOD with pH, Ca hardness and temperature, total hardness with Ca hardness, conductivity with total hardness, while significant positive correlation at p < 0.01 level was observed for alkalinity with Mg hardness (Table 5). The highly significant correlation between BOD and pH is due to high microbial decomposition taking place in Fig. 9 a Monthly variation in conductivity from the 4 sampling stations. b Seasonal variation in conductivity from the 4 sampling stations the water bodies followed by formation of carbonic acid imparting acidity nature. During rainy season, conductivity was found to be strongly interrelated with temperature (r 2 = + 0.834), Cl (r 2 = −0.947), alkalinity (r 2 = + 0.913), Ca hardness (r 2 = + 0.915) and Mg hardness (r 2 = −0.656). Free CO 2 shows strong relation with temperature, chlorine and DO (r 2 = −0.842, + 0.887 and + 0.938). Alkalinity was found to correlate strongly with temperature, DO, Cl, pH with r 2 = 0.934, −0.866, −0.794, −0.807 and −0.827, respectively. Total hardness shows strongly interrelated with BOD and temperature (r 2 = −0.909 and −0.889) as illustrated in Table 3. Electrical conductivity was found to be significant with alkalinity which may be due to the presence of chloride ion imparting as electrolytes. In summer, a strong correlation exists between free CO 2 with DO (r 2 = − 0.858), chlorine with DO (r 2 = 0.838), total hardness with temperature (r 2 = 0.825) as shown in Table 5. In winter, strongly interrelated variables in physico-chemical parameters were observed for free CO 2 with temperature, pH and BOD (r 2 = 0.832, − 0.943 and − 0.847). Chlorine was found to correlate strongly with DO (r 2 = − 0.792) as given in Table 5. Positive correlation for free CO 2 with temperature is due to low temperature in winter lowering the activities of microbial decomposition accompanied with less respiration. Negative correlation of free CO 2 with pH indicates that with increase in CO 2 , carbonic acid is formed, hence imparting acidity nature of water.

Arsenic
The presence of toxic arsenic in drinking water has been an inevitable concern for many years due to its occurrence in natural environmental media usually present in the form of compounds with sulfur and with many metals. Apart from that naturally occurring in spring waters, arsenic is primarily sourced from industrial discharges and also enters into ground water through underground rocks, Fig. 11 a Monthly variation in calcium hardness from the 4 sampling stations. b Seasonal variation in calcium hardness from the 4 sampling stations soil and industrial human activities. Increased concentration of arsenic can cause problems to skin, circulatory and nervous system. Daily consumption of water with greater than 0.01 mg/l of As, less than 0.2% of the fatal dose, can on long term lead to problems with the skin as well as circulatory and nervous systems. The maximum acceptable concentration of arsenic in drinking water recommended by the World Health Organization is 10 µg/l. In our study none of the samples were found detected the presence of arsenic.

Cadmium
Cadmium is uniformly distributed in trace amounts in the earth's crust and is highly toxic and responsible for several cases of food poisoning. Small quantities of Cd cause adverse changes in the arteries of human kidneys. Cadmium enters water through industrial discharges or the deterioration of galvanized pipes and can be present in groundwater from a wide variety of sources in the environment and from industry. In the present study, all samples have been found to be exceeding the WHO standards. The concentration of Cd in all the four sampling stations was 0.02 mg/l (Table 6) which is higher than the permissible limit given by WHO (0.005 mg/l) and ISI (0.01 mg/l). The concentration of Cd in all the sampling station is higher than the value obtained by Jamir et al (2011) in five district of Nagaland (0.001 to 0.002 mg/l). Satarug et al. (2003) show a broad review of the possible sources of Cd globally. The sources of Cd at S1 and S4 may be due to disposal of various electronic wastes along with solid waste without any proper segregation and treatment from the nearby residential zones of the village, institutional quarters and hostels. Presence of Cd at S2 may be due to corrosion of galvanized iron pipes used for carrying water and at S4 could be associated with the possibility of rock-water interaction in deep aquifers along with runoff from the institutional residential quarters and hostels.

Copper
Even though Cu is an essential element in human metabolism, intake of excessively large doses leads to severe mucosal irritation and corrosion wide spread capillary damage, hepatic and renal damage and central nervous system irritation followed by depression. The concentration of Cu was found in only two stations, i.e., S1 and S3 with values 0.003 mg/l and 0.001 mg/l, respectively. However, S2 and S3 were below detection limit (BDL) as shown in Table 6. Concentration of the Cu is very less compared to the value obtained by Jamir et al (2011)  Hence, the concentration of the copper in all the sampling station is less than the permissible limit given by WHO and ISI. Rozan et al. (1999) showed that geochemical factors can affect the Cu concentration. Therefore, the possible cause of the increase and pollutant factors of soil, sediments and biota need to be analyzed to determine the accurate reason of the Cu pollution in the studied sites.

Iron
Iron is a naturally occurring metal in nature in the form of magnetite, hematite, etc., and enters into water in the extraction of metal from its ore. It also enters into the water when aluminum waste products containing iron are discharged into water. The concentration of Fe (mg/l) in the four sampling stations followed the trends as S3 (2.0) > S4 (0.65) > S1 (0.17) > S2 (0.02) as shown in Table 6. From our study it was observed that the concentration of Fe at S3 and S4 is beyond the permissible limit given by WHO and ISI (0.3 mg/l). However, S1 and S2 are below the permissible limit of WHO and ISI. Overall, our results for iron are very high compared with the work of Jamir et al (2011) in Lumami wherein the values ranged from 0.02 to 0.18 mg/l. According to Singh (2004), the amount of iron is relatively high and almost all the states in northeast region contain iron above the permissible level in drinking water. The excess concentration of iron at S3 and S4 may be attributed to rocks and soil containing iron, old pipe of water supply system which can dissolve into the water source. Even though iron poses no danger to health at levels found in drinking water, it may affect acceptability of drinking water by altering its appearance, taste, odor, stain clothing and appliances and promote the growth of iron bacteria in the water system (WHO 2000).

Lead
Lead is a naturally occurring toxic metal used in many domestic products and enters into water through the corrosion of pipes. In our present study, none of the water samples found detected the presence of Pb in the sampling stations.

Manganese
Manganese is an essential trace element abundantly present in earth crust in the form of oxides and hydroxides. Manganese commonly finds its way into water through activities such as industrial emissions, soil erosion, volcanic emissions and the burning human activities. Manganese solids may form deposits within pipes and break off as black particles giving water an unpleasant taste and appearance. It increases the growth of unwanted bacteria that form a slimy coating in water pipes and reservoir (Lemly 2002). The concentration of Mn (mg/l) in the four sampling stations followed the trends as S3 (0.036) > S1 (0.014) > S2 (0.013) > S4 (0.011) as given in Table 6. Values were found to be lower compared to the report by Jamir et al (2011) at Lumami (0.12 to 0.21 mg/l) in Nagaland.

Mercury
In the present study none of the sample were found to exceed the WHO and ISI standard though the concentrations at 0.001 mg/l S1, S2 and S4 lie to the maximum permissible limit given by ISI (0.001 mg/l). The concentrations of Hg in three sampling stations, viz., S1, S2 and S4, recorded the value of 0.002 mg/l which is beyond the permissible limit of ISI (0.001 mg/l) as seen in Table 6. Compared with our results the values of Hg in water samples of the 4 station were below the permissible limit of WHO (2008). Maximum concentration of Hg in S1 (stream water), S2 (tap water) and S4 (spring water) may be attributed due to natural deposits as the area around the University campus has no evident of mining activities.

Selenium
Selenium in drinking water can come from discharge from mines, refineries, natural deposits or from agricultural runoff leaching natural selenium compounds from dry, undeveloped land. Prolonged exposure to selenium above the maximum contaminant level can cause fingernail or hair loss, numbness in extreme cases and circulation problems. EPA (Environment Protection Agency) has set the selenium standard for drinking water at 0.05 mg/l to protect consumers served by public water systems from the effects of longterm, chronic exposure to selenium. The concentration of Se (mg/l) in the four sampling stations followed the trends as S3 and S4 (0.013) > S2 (0.012) > S1 (0.009) as given in Table 6. The values of Se concentrations in all the four station lie within the permissible limit given by WHO and ISI (0.01 mg/l). The levels of selenium in groundwater and surface water range from 0.06 µg/l to about 400 µg/l (Scott et al. 1961;Lindberg 1968). In some areas, selenium levels in groundwater may approach 6000 µg/l (Cannon 1964). Concentrations increase at high and low pH as a result of conversion into compounds of greater solubility in water. Levels of selenium in tap water samples from public water supplies around the world are usually much less than 10 µg/l but may exceed 50 µg/l (NAS 1976(NAS , 1977Gore et al 2010).

Zinc
Zinc leaks from zinc pipes and rain pipes, consequential to circulation of carbon-rich water. Increase concentration of zinc may cause toxicity leading to stomach aches, vomiting, colics, fevers and diarrhea (Wang et al. 1991). In our present study none of the samples exceed WHO, ISI and BIS standards. The concentration of Zn (mg/l) in all the sampling stations followed the trend as S2 (0.7) > S4 (0.026) > S3 (0.03) > S1 (0.06) as given in Table 6. Value of Zn at S2 is comparatively similar with the work of Jamir et al (2011) in Lumami, Zunheboto district of Nagaland where they obtained the concentration of Zn ranges from 0.45 to 0.78 mg/l. Higher concentration of Zn from S2 may be attributed due to dissolution of Zn from water pipelines.

Conclusion
The results of the physico-chemical parameters of the water show that value of DO, BOD 5 and free CO 2 at S3 is beyond the permissible limit set by WHO and BIS. The values of DO in all the three stations (S1, S2 and S4) are near to the maximum permissible limit of drinking water, indicating the deterioration of water quality, whereas the rests of the parameters are below the permissible limit of WHO and BIS.
Correlation studies between the variables of the physico-chemical parameters indicate that during rainy season, DO was found to be highly significant with chlorine, free CO 2 , with total alkalinity, Ca hardness and conductivity, total alkalinity with Ca hardness and total hardness with Mg hardness. In summer, pH was found to be highly significant with Cl, BOD, with free CO 2 . In winter, temperature was found to be highly significant Ca hardness, total alkalinity with Mg hardness and total hardness with Ca hardness. These relationships show that DO and free CO 2 are the most important parameters of water quality parameters which indicate the pollution status of water quality. The results of the concentrations of some heavy metals such as Cu, Zn and Mn in all the 4 sampling stations are below the maximum permissible limit prescribed by WHO and ISI. The concentration of Cu is below the detection limit at S2 (tap water) and S4 (spring water), whereas the concentration of As and Pb was not detected in all the 4 stations. The concentrations of Hg were detected in all 4 stations, and the values were higher than the maximum permissible limits ISI (0.001 mg/l) but lower than WHO limit, i.e., 0.010 mg/l. Hg level was similar in all the 4 stations. The concentrations of Fe were beyond the maximum permissible limit at S3 (well water) and S4 (spring water), whereas at S1 (stream water) and S2 (tap water) the values were below the permissible limit prescribed by WHO and ISI, i.e., 0.3 mg/l.
From the study, we can conclude our hypothesis that except well water rest of the water sources, namely spring, stream and tap water, are not much polluted and fit for domestic uses. However, the detection of Hg and Fe in all the four types of water sources is an indication of contaminant hazards which, in turn, have implications on the human health.
This study, therefore, recommends the government and other responsible authorities of the area to: (1) introduce relevant drinking water treatment techniques which can mitigate the current levels of heavy metals, (2) prevent any kind of waste disposal into streams, or any reservoirs that supply domestic drinking water, (3) educate the people to have better drinking water storage practices, (4) support for further study to investigate into biological parameters of significant health concern and (5) lastly, soil and crops cultivated in the vicinity of the areas need to be evaluated for the detection of PTEs.