Negative Prognostic Implication of TERT Promoter Mutations in Human Papillomavirus–Negative Tonsillar Squamous Cell Carcinoma Under the New 8th AJCC Staging System

Telomerase reverse transcriptase gene promoter (TERTp) mutation is a potential candidate for pathogenesis and therapeutic target of tonsillar squamous cell carcinomas (TSCCs) in association with human papillomavirus (HPV). Their clinical relevance has not been validated under the new 8th American Joint Committee on Cancer (AJCC) staging system. We analyzed real-time peptide nucleic acid–mediated PCR and sequencing methods (TERTp mutation) and real-time PCR-based assay (HPV) in 80 surgically resected TSCCs. The 8th edition staging system improved the stratification of the early and advanced stages and between T or N categories for overall survival over the 7th edition. TERTp mutation was found in 7.5%, and HPV in 80.0% of the patients. The majority (83.3%) of TERTp mutation cases were HPV-positive TSCCs. Applying the 8th edition staging system, TERTp mutation was an independent factor of poor prognosis for disease-free survival (DFS) in TSCC patients, supporting the clinical significance of TERTp mutation in tonsil cancer. TERTp mutations were also negatively correlated with overall survival and DFS in HPV-negative TSCCs. Conclusively, TERTp mutation provides negative prognostic impact on survival of surgically managed tonsil cancers staged with the AJCC 8th edition.


Introduction
Tonsillar squamous cell carcinomas (TSCCs) account for 70-80% of the oropharyngeal cancers most prevalent for human papillomavirus (HPV) [1][2][3]. In oropharyngeal squamous cell carcinomas (SCCs), HPV is associated with better prognosis and response to radiochemotherapy compared with HPVnegative oropharyngeal SCCs [3,4]. Tonsils are the subsite of oropharyngeal cancers with the highest HPV-positive rate [2,3]. The 5-year survival in early cases has been reported to be > 90%, which decreases to < 20% in advanced tonsil cancer [1][2][3]5]. Both primary surgery and radiotherapy/ chemotherapy are effective treatments for TSCCs [4]; however, treatment failures can develop unexpectedly. The 8th edition of the American Joint Committee on Cancer (AJCC) staging system for oropharyngeal cancer incorporates HPV infection and extranodal extension [6], but it is unclear whether those result in better stratification of Korean patients, as the area has relatively low incidence of oropharyngeal cancer [7][8][9]. Because risk stratification, prognosis prediction, treatment selection, and follow-up strategies often depend on the AJCC system, validation of staging is crucial.
The TERT promoter (TERTp), a critically important regulatory element for telomerase expression harboring binding sites for a number of transcriptional activators and repressors, contributes to increased telomerase activity that leads to immortalization of cells, which is one of the hallmarks of cancer [10,11]. Two mutually exclusive G-A mutations at nucleotide − 124 and − 146 within the core promoter region of TERT gene occur as a pathogenic mutation in human malignancies [12], which are also driver mutations in head and neck SCCs [13][14][15][16][17]. The TERTp mutation, alone and with the HPV oncogenes, plays an important role in oral and uterine cervical SCCs [14,18,19]. Two viral oncogenes, E6 and E7, expressed by high-risk HPV-associated cancers, affect the oncogenic pathway related to cellular immortalization, typically activating telomerase expression [18,20,21]. Although previous studies examined multiple subsites of oral or oropharyngeal cancers, the frequencies of TERTp mutation in tonsil cancers and the association to HPV have rarely been investigated in the series of their studies [22,23]. Furthermore, the revised 8th AJCC staging system in terms of oropharyngeal cancers with or without HPV has not been validated in the Far East Asian cohort data, specifically on tonsil cancers.
Here, we investigated TERTp mutation, HPV infection, and clinicopathological characteristics in 80 Korean primary TSCC patients. We sought to validate the 8th edition of the AJCC staging system compared with the 7th edition, with variation in patient location and therapy.

Patients
Formalin-fixed, paraffin-embedded (FFPE) tissues were obtained from 80 TSCC patients who underwent primary resection, with no prior treatment and complete medical records at our institution between 1997 and 2018. Clinical information was analyzed using medical records and radiological results. Heavy smoking was defined as > 20 pack-years [6]. Alcohol consumption was defined as > 14 drinks/week [6]. Of these 80 patients, 11 patients underwent postoperative radiotherapy, 2 patients had chemotherapy, and 39 patients had chemoradiotherapy following the surgical resection. The remaining 28 patients were treated with surgery alone. Radiation doses ranged from 5040 to 7200 cGy/36 fractions over 8 weeks.
Diagnosis and histological differentiation were evaluated according to the World Health Organization classification [1]. Patients were re-staged according to the 8th editions of the AJCC/UICC TNM classification [6]. The study protocol was approved by Sacred Heart Hospital Institutional Review Board (No. 14-2-57) and performed in accordance with the relevant guidelines and regulations (Declaration of Helsinki). Informed consent was obtained from the patients and from the next of kin (deceased patients) before enrollment in the study.

DNA Extraction and Detection of TERT Promoter Mutation
Genomic DNA was extracted from 10-μm-thick sections of 10% neutral FFPE tumor tissue blocks using Maxwell 16 FFPE Tissue LEV DNA Purification Kit for DNA (Promega, USA). TERTp mutations (C250 and C228) were identified using the PNAClamp™ TERT mutation detection kit (PANAGENE, Daejeon, South Korea [10]. Subsequently, the TERTp mutation analyses were also confirmed by directional sequencing of PCR fragments amplified from genomic DNA. The primers used for TERTp were as follows [24]: forward, 5′-AGTGGATTCGCGGGCACAGA-3′, and reverse, 5′-AGCACCTCGCGGTAGTGG-3′, which amplified a 346 bp fragment. PCR amplification was carried out in a reaction volume of 30 μl containing 100 ng of template DNA, 10× PCR buffer, 0.25 mM dNTPs, 10 pmol primers, and 1.25 U Taq DNA polymerase (Solgent, Korea). The thermal cycling conditions were as follows: denaturation at 95°C for 3 min, followed by 10 cycles of 95°C denaturation for 30 s, 60°C annealing for 30 s, and 68°C elongation for 1 min. This was followed by 30 cycles under the same settings, with the elongation step modified to continue for an additional 5 s each cycle. PCR was completed with final elongation at 68°C for 7 min. PCR products were electrophoresed on 2% agarose gels and purified with a Solgent PCR purification kit (Solgent). All amplification products were sequenced bidirectionally using an automated sequencer (ABI 3130xl; Applied Biosystems, Foster City, CA, USA) using the BigDye Terminator v1.1 kit (Applied Biosystems) and the appropriate forward and reverse primers.

Detection of HPV
HPV status was evaluated by PANA RealTyper HPV genotyping kit and PANA RealTyper HPV screening kit (PANAGENE). This kit, approved for clinical use in Korea, detects a total of 40 HPV genotypes including 20 high-risk genotypes (16,18,26,31,33,35,39,45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 70, 73, and 82), 2 low-risk genotypes (6 and 11), and 18 other genotypes. Briefly, real-time PCR assays were performed in a 25 μl reaction mixture containing 19 μl of HPV mix, 1 μl of Taq DNA polymerase, and 5 μl of extracted DNA, positive control, or negative control. PCR was performed using the following conditions: 1 cycle of incubation at 50°C for 2 min and Taq activation at 95°C for 15 min; 45 cycles of denaturation at 95°C for 15 s, annealing at 55°C for 45 s, and extension at 72°C for 15 s; and a melting curve step at 95°C for 5 min, 35°C for 5 min, followed by increase in temperature from 35°C to 80°C for 5 min, with a gradual increment of 0.5°C (every 5 s) to achieve fluorescence in all four channels (FAM, HEX or VIC, ROX, and Cy5).

Statistical Analysis
Correlations between the TERTp mutation and clinicopathological variables were assessed using the Chi-squared test or two-tailed Fisher's exact test. Factors found to be significant in univariate analysis were included in subsequent binary logistic regression analysis to identify independent variables associated with TERTp mutation. Survival analyses were performed using the Kaplan-Meier method and were compared using a log-rank test. Overall survival (OS) was defined as the interval from the first day of surgery until death. Disease-free survival (DFS) was defined as the interval from the first day of surgery until tumor recurrence. OS and DFS were analyzed until February 2019. Univariate and multivariate analyses using the Cox proportional hazard regression model were applied to determine the hazard ratio (HR) and 95% confidence intervals (CI) for specific variables related to OS and DFS. SPSS version 18 (SPSS Inc., Chicago, IL, USA) was used for all statistical analyses. P values < 0.05 were considered statistically significant.
We performed Kaplan-Meier survival analyses of OS in 80 patients who had previous TNM information according to AJCC 8th vs. AJCC 7th staging system (Fig. 1). We compared the T category, N category, and overall stages assigned by the 8th and 7th editions of the AJCC staging system. As shown in Fig. 3, the 7th edition of the AJCC staging system performed poorly with respect to the discrimination and stratification of N category and overall stages (P = 0.063 and P = 0.471, respectively). Only the T category was well discriminated according to clinical outcomes (P = 0.043). In contrast, the 8th edition provided statistically significant stratification for the T category, N category, and overall staging (P = 0.041, P < 0.001, and P < 0.001, respectively). Therefore, the 8th edition was applied in our study.
We analyzed the associations of HPV or TERTp mutation with clinical and pathological features of 80 TSCCs ( Table 1). The presence of HPV was more frequently associated with younger age (≤ 60 years) (P = 0.010), low alcohol consumption (P = 0.021), pNpositive status (P < 0.001), lower AJCC stage (P = 0.020), and presence of ipsilateral lymph node metastasis (P = 0.004). HPV positivity was not associated with TERTp mutation. There were no statistical associations between TERTp mutation and clinicopathologic features of TSCCs.

Prognostic Correlation of TERTp Mutation
In Kaplan-Meier survival analyses, TERTp mutation was found to be statistically associated with shorter DFS rates than those of TERTp wild-type (P = 0.007), whereas no statistical difference was observed for OS between TERTp mutation and TERTp wild-type (P = 0.983) (Fig. 3b, c). We further analyzed the prognostic impact of TERTp mutations on OS and DFS according to HPV status. TERTp mutations were strongly correlated with decreased OS and DFS in patients with HPVnegative TSCCs (P = 0.005 and P = 0.018, respectively) (Fig. 3d, e). However, there were no prognostic correlations of TERTp mutations with OS or DFS TERTp mutations in patients with HPV-positive TSCCs (P = 0.202 and P = 0.904, respectively) (Fig. 3f, g).

Discussion
In the present study, the new staging system improved stratification of early and advanced stages and between T or N category concerning overall survival based on Korean population. Under the new AJCC 8th edition, the prognostic implication of TERTp mutation in tonsil cancers has been rarely reported. Applying the new AJCC 8th edition, we confirmed the negative prognostic impact of TERTp mutation on survival of surgically managed tonsil cancers staged with the new AJCC 8th edition, especially on HPV-negative TSCCs. TERTp mutation has been reported as a predictor of poor prognosis in laryngeal cancers but not in oral SCCs [17,25]. A meta-analysis based on published articles concluded that TERTp mutation serves as an adverse prognostic factor in any cancer regardless of organ [26]. Overexpression of TERT by its promoter mutation representing late events of the oncogenic process may increase the self-renewal capacity of cancer stem cells and induce poor clinical outcomes [27,28]. The non-canonical functions of TERTp mutation might biologically sustain how TERTp mutation is related to poor prognosis.
Vinagre et al. [29] divided various tumors into those with a high frequency of mutations (≥ 5%) and tumors with no mutations or with a very low frequency of TERTp mutations (< 5%) because mutations affecting the telomerase coding region are very uncommon in the cancer setting [11]. We observed 7.5% TERTp mutation in TSCCs, which is a relatively high frequency according to Vinagre et al. [29]. The frequency of TERTp mutation is variable in head and neck SCCs, which could be because the TERTp mutation can also result from  HPV human papillomavirus, p pathologic, LN lymph node, BOT base of tongue, AJCC American Joint Committee on Cancer, ENE extranodal extension *Statistically significant, P < 0.05 environmental factors such as ultraviolet radiation and chemical carcinogens [29,30]. Thus, oral cavity SCCs have a wide range of the frequency of 0-65% for the TERTp mutation [14,17,22,23]; this mutation is also common for tongue area (63.6%) and laryngeal SCCs (27%) [22,25]. The lack of specific clinicodemographic features related to TERTp mutation, found in the present study, has been mainly described in SCCs in head and neck or other sites including skin, lung, and uterine cervix [14,22,25,31]. The majority (83.3%) of TERTp mutations occurred exclusively in the HPV16-positive TSCCs, although there was no statistical association between TERTp mutation and HPV. The considerable presence of TERTp mutation in HPV-positive tumors but a lack of statistical association between them have been previously described in the oral, oropharyngeal, and uterine cervical cancers, where all HPV16-positive oral SCCs and 70% Fig. 3 a Representative H&E images according to HPV and TERTp mutation (magnifications, × 200). TERTp mutation is not related to overall survival (b), whereas it has a strong prognostic impact on shorter disease-free survival (c). In HPV-negative tumors, TERTp mutation is associated with worse overall survival (d) and disease-free survival (e). However, there are no survival correlations in terms of overall (f) and disease-free (g) survivals in HPV-positive tumors of HPV-positive uterine cervical cancers harbored TERTp mutations [14,22]. Some previous studies assumed that HPVinduced TERTp activations would be functionally different from the consequences of TERTp mutation in the absence of HPV [11,[18][19][20].
This study thoroughly investigated HPV status and genotypes in clinical specimens and stratified patients according to HPV status, with long-term follow-up data. In the present study, only high-risk HPV was dominated (80%) with the majority (92.2%) of HPV16. High-risk HPVs are the cause of approximately 31% of oropharyngeal SCCs [2,9]. The prevalence of HPV infections in TSCCs is 37-80% in western countries and 35-73% in Korea [3,7,21,32,33].
HPV16 is the dominant virus in oropharyngeal SCC accounting for 82-87% of all HPV-positive cases [9]. The fraction of oropharyngeal cancer attributable to HPV is similarly the highest (> 40%) in developed countries including Europe, North America, Australia, New Zealand, Japan, and Republic of Korea [9,34]. HPV infection was not an independent prognostic factor for OS and DFS in the 8th edition in the present study, which may be because of the incorporation of HPV into T and N categories of the staging system, and the effect of HPV might have been overshadowed by the impact of the staging modification because our study analyzed all together with HPV-positive and HPV-negative tumors.
In conclusion, the present study reveals that the TERTp mutation is present in a subpopulation of patients with TSCC and emphasizes the negative prognostic impact of TERTp mutation in tonsil cancers under the newly 8th edition of the AJCC staging system for oropharyngeal cancers, especially on HPV-negative TSCCs. This may be utilized to determine clinical aggressiveness of TSCCs.
Author Contributions MJK and HK designed the study, interpreted the data, wrote the manuscript, and drafted of the manuscript; ESN, SJC, K-WM, and MH participated in study coordination and pathologic data analysis; BP, HGC, ESK, HSH, TK, and HJK collected clinical samples and analyzed clinical data. All authors read and approved the final manuscript.

Data Availability
The data used to support the findings of this study are available from the corresponding author upon request.

Compliance with Ethical Standards
Declarations Not applicable.

Conflict of Interest
The authors declare that they have no conflict of interest.
Ethics Approval The surgical materials were collected and used according to the instructions of Kangdong Sacred-Heart Hospital Institutional Ethics Committee (No. 14-2-57). Specimens and data were stored anonymously.
Consent to Participate Informed consent was obtained from all individual participants included in the study.
Consent for Publication Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.
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