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Correlation of hsa miR-101-5p and hsa miR-155-3p Expression With c-Fos in Patients of Oral Submucous Fibrosis (OSMF) and Oral Squamous Cell Carcinoma (OSCC)

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Abstract

Aim

MicroRNAs have been widely acknowledged as a diagnostic, prognostic, and/or therapeutic biomarker for the progression of OSCC, but the correlation of hsa-miR-101-5p and hsa-miR-155-3p is yet to be established with c-Fos in OSCC and OSMF.

Methodology

An observational study enrolled 40 patients divided into 2 groups: Group I—21 OSMF patients without malignant transformation, Group II—19 patients with locally advanced, large-operable, or metastatic OSCC, after applying inclusion and exclusion criteria. Both miRNAs were extracted and analyzed from the tissue sample excised from the involved site. The linear regression analysis of the expression of hsa-miR-155-3p, hsa-miR-101-5p, and levels of c-fos in OSMF and OSCC patients and its correlation for habits, age, and gender were evaluated.

Results

The expression of hsa-miR-101-5p was 0.81 times downregulated in OSCC tissue compared to OSMF, whereas hsa-miR-155-3p and c-fos were both upregulated 9.30 times and 1.75 times, respectively, in OSCC tissue. In Gutkha and tobacco chewers, the hsa-miR-155-3p expression could explain 12.3% (p = 0.031) for Gutkha chewers, whereas c-fos could explain 38.6% of the cases (p = 0.020) for tobacco chewers. The expression of hsa-miR-101-5p and hsa-miR-155-3p explained 43.7% and 59.5% of OSCC cases in alcoholics, respectively. Interestingly, in non-alcoholics, hsa-miR-155-3p and hsa-miR-101-5p were significant predictors of OSCC.

Conclusion

Downregulation of tumor-suppressor hsa-miR-101-5p and upregulation of proto-onco hsa-miR-155-3p is responsible for intricate regulation of the progression of OSMF to OSCC via deregulated expression of c-Fos and tobacco chewing and advancing age is significant contributors for OSCC.

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Acknowledgements

We would like to extend our thanks to our study participants for participating in the study. We also like to thank our parent Institute for funding this study.

Funding

This study received funding from the parent institute (Grant Number AIIMS/IEC/2016/238).

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Authors and Affiliations

  1. Department of Dentistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Ankita Chugh, Amanjot Kaur & P. G. Gigi

  2. Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Purvi Purohit, Anupama Modi & Praveen Sharma

  3. Department of Surgical Oncology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Jeewan Ram Vishnoi & Sanjeev Mishra

  4. Department of General Surgery, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Mahaveer Singh Rodha

  5. Department of Radiotherapy, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Puneet Pareek

  6. Department of Pathology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India

    Shilajit Bhattacharya

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  1. Ankita Chugh
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Correspondence to Ankita Chugh.

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Parent Institute Where Research Was Conducted: All India Institute of Medical Sciences, Jodhpur, Rajasthan, INDIA

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Chugh, A., Purohit, P., Vishnoi, J.R. et al. Correlation of hsa miR-101-5p and hsa miR-155-3p Expression With c-Fos in Patients of Oral Submucous Fibrosis (OSMF) and Oral Squamous Cell Carcinoma (OSCC). J. Maxillofac. Oral Surg. 22, 381–387 (2023). https://doi.org/10.1007/s12663-021-01668-0

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