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Proteolysis Coupled with Membrane Separation for the Isolation of Bioactive Peptides from Defatted Smooth Hound Byproduct Proteins

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Abstract

The defatted viscera from smooth hound were the raw material used to produce four protein hydrolysates using three different microbial proteases (Neutrase, Esperase and Purafect) and the endogenous enzymes, named VPH-N, VPH-E, VPH-P and VPH-EE, respectively. Hydrolysates showed different degrees of hydrolysis (DH) depending on the enzyme used, where the VPH-P had the highest DH (30%). The amino acids (AA) characterization showed that Gly was the prominent AA (≥ 22%), followed by Ala, Glx, Lys, Asx, and Pro. In addition, the assessment of the antioxidant, ACE-inhibitory and antimicrobial activities revealed the high potentiality of the Purafect-hydrolysate. Therefore, it was selected to be fractionated by ultra-filtration according to their molecular weight (MW). Data showed that FIV (MW < 5 kDa) exhibited the best antioxidant and ACE inhibition potentials. The differences detected in the bio-activities may be attributed, not only to the peptides size, but also to their sequences and hydrophobic AA contents. The antibacterial activity showed that the best inhibition values were recorded with FIV, particularly against S. typhi. Thus, the present results demonstrated the high potential of low MW peptides from viscera hydrolysate to be used as a promising natural source of bioactive agents in functional food formulations.

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All analysis and data of the present study are included in this published article.

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Acknowledgements

This work was supported by the Ministry of Higher Education and Scientific Research, Tunisia.

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Authors and Affiliations

  1. Laboratory of Functional Physiology and Valorization of Bio-Resources (LR17ES27), Higher Institute of Biotechnology of Beja (ISBB), University of Jendouba, 9000, Beja, Tunisia

    Ola Abdelhedi & Mourad Jridi

  2. Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, B.P. 1173-3038, Sfax, Tunisia

    Ola Abdelhedi & Moncef Nasri

  3. Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino, 7, 46980, Paterna, Valencia, Spain

    Leticia Mora & Fidel Toldrá

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  1. Ola Abdelhedi
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Abdelhedi, O., Mora, L., Jridi, M. et al. Proteolysis Coupled with Membrane Separation for the Isolation of Bioactive Peptides from Defatted Smooth Hound Byproduct Proteins. Waste Biomass Valor 15, 1959–1974 (2024). https://doi.org/10.1007/s12649-023-02303-4

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