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Preparation of Environmental Probiotic Factor γ-Polyglutamic Acid via Microbial Fermentation Using Baijiu Brewing Steamer Bottom Water as a Basic Substrate

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Abstract

Steamer bottom water is the main component of wastewater in the Baijiu industry, Find a suitable method to deal with and use the Steamer bottom water. A new, cheap raw material and process of γ-PGA production were developed. In this study, the single factor and response surface design were used to optimize the medium and conditions for high-yield γ-PGA by submerged fermentation of bottom pot water with the concentration of γ-PGA as the detection object. The volatile components in fermentation broth were detected by GC–MS. The fermentation medium formula and addition amount were determined to be 60 g/L corn saccharification liquid, 8 g/L peptone, 1 g/L K2HPO4, and 0.04 g/L MnSO4·H2O. The inoculation amount was 2%, 37 °C, 150 r/min, and the fermentation was 72 h. The γ-PGA yield was 48.29 g/L, which was 1.98 times higher than that before optimization. We detected 12 volatile substances in the fermentation broth, acetic acid, 2,3-butanedione, 2-methyl-butanoic acid, and acetoin were recognized as Plant probiotic factors. The N, P, K, and total nutrient (sum of N, P, and K) contents were 4100, 3405, 885, and 8390 mg/L, respectively; the number of viable Bacillus subtilis was 4.5 × 107 CFU/mL. Teamer bottom water of Baijiu brewing can completely replace water as a fermentation medium solvent to produce γ-PGA. Moreover, the fermentation broth is a potential functional organic fertilizer.

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Acknowledgements

We gratefully acknowledge the Yibin Wine Factory for providing steamer bottom water. We also thank Editage (www.editage.cn) for English language editing.

Funding

This work was supported by the Innovation and Entrepreneurship Project of Sichuan University of Science and Engineering (cx2021142), Solid State Fermentation Resources Utilization Sichuan Key Laboratory Open Fund Basic Research Project (2021GTJC05), and Yibin Wuliangye Co., Ltd (CXY2019ZR016).

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Authors and Affiliations

  1. College of Biotechnology, Sichuan University of Science and Engineering, Baita Road 1, Sanjiang New District, Yibin, 644000, Sichuan, China

    Fengqing Wang, Can Yang, Yanmei Chen, Mengxue Li & Changfu Bi

  2. Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province, Yibin, 644000, China

    Fengqing Wang

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  1. Fengqing Wang
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Correspondence to Fengqing Wang.

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Wang, F., Yang, C., Chen, Y. et al. Preparation of Environmental Probiotic Factor γ-Polyglutamic Acid via Microbial Fermentation Using Baijiu Brewing Steamer Bottom Water as a Basic Substrate. Waste Biomass Valor 15, 2323–2332 (2024). https://doi.org/10.1007/s12649-023-02299-x

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