Skip to main content

Advertisement

SpringerLink
Log in

Green Immobilization of Glucanobacter xylinum onto Natural Polymers to Sustainable Bacterial Cellulose Production

  • Original Paper
  • Published:
Waste and Biomass Valorization Aims and scope Submit manuscript

Abstract

Bacterial cellulose (BC) has profound applications in different sectors of biotechnology due to its unique properties which made it preferring it about plant cellulose. Although this polymer is extremely important in various applications, many problems still hinder the sustainable production in terms of increasing productivity and low-cost production. In order to overcome these problems, the continuous production potentiality of cellulose using immobilized Glucanobacter xylinum cells onto Sugar cane bagasse (SCB) and Ca-alginate beads will be evaluated. Comparatively, adsorption of Glucanobacter xylinum cells to the cavum of stalk cells of SCB could be efficiently stable while, entrapment of cells onto Ca-alginate has drawback observed by the rapid disruption and instability of the beads in the Potato Peel Waste (PPW) culture medium. Furthermore, the FT-IR, XRD and SEM analysis of the BC derived from immobilized cells on SCB observed a higher crystallinity (86%) than that produced from immobilized cells on alginate beads. Consequently, BC production was statistically optimized by SCB-immobilized cells using Plackett–Burman Design. Among seven selected variables, incubation period and pH value were found to be the highest significant parameters. Reusability of immobilized biomass was studied and showed continuous BC production even after five cycles without losing their activity. Our findings demonstrate that a combination between alternative low-cost medium with continuous production mode by immobilization onto inexpensive natural polymer can promote a sustainable bioprocess and reduction the production cost.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Hasanin, M.S., Mostafa, A.M., Mwafy, E.A., Darwesh, O.M.: Eco-friendly cellulose nano fibers via first reported Egyptian Humicola fuscoatra Egyptia X4: isolation and characterization. Environ. Nanotechnol. Monit. Manage. 10, 409–418 (2018)

    Google Scholar 

  2. Abdelraof, M., Ibrahim, S., Selim, M.A., Hasanin, M.: Immobilization of L-methionine γ-lyase on different cellulosicmaterialsand its potential application in green-selective synthesis of volatile sulfur compounds. J. Environ. Chem. Eng. 8, 103870 (2020)

    Article  Google Scholar 

  3. Hasanin, M.S., Al Kiey, S.A.: Environmentally benign corrosion inhibitors based on cellulose niacin nano-composite for corrosion of copper in sodium chloride solutions. Int. J. Biol. Macromol. 161, 245 (2020)

    Article  Google Scholar 

  4. Abdelraof, M., Hasanin, M.S., El-Saied, H.: Ecofriendly green conversion of potato peel wastes to high productivity bacterial cellulose. Carbohyd. Polym. 211, 75–83 (2019)

    Article  Google Scholar 

  5. Abdelraof, M., Hasanin, M.S., Farag, M.M., Ahmed, H.Y.: Green synthesis of bacterial cellulose/bioactive glass nanocomposites: effect of glass nanoparticles on cellulose yield, biocompatibility and antimicrobial activity. Int. J. Biol. Macromol. 138, 975–985 (2019)

    Article  Google Scholar 

  6. Hasanin, M.S., Darwesh, O.M., Matter, I.A., El-Saied, H.: Isolation and characterization of non-cellulolytic Aspergillus flavus EGYPTA5 exhibiting selective ligninolytic potential. Biocatal. Agric. Biotechnol. 17, 160–167 (2019)

    Article  Google Scholar 

  7. Watanabe, K., Tabuchi, M., Morinaga, Y., Yoshinaga, F.: Structural features and properties of bacterial cellulose produced in agitated culture. Cellulose 5(3), 187–200 (1998)

    Article  Google Scholar 

  8. Kowalska-Ludwicka, K., Cala, J., Grobelski, B., Sygut, D., Jesionek-Kupnicka, D., Kolodziejczyk, M., Bielecki, S., Pasieka, Z.: Modified bacterial cellulose tubes for regeneration of damaged peripheral nerves. Arch. Med. Sci. 9(3), 527 (2013)

    Article  Google Scholar 

  9. Chen, S., Huang, Y.: Bacterial cellulose nanofibers decorated with phthalocyanine: preparation, characterization and dye removal performance. Mater. Lett. 142, 235–237 (2015)

    Article  Google Scholar 

  10. Bilgi, E., Bayir, E., Sendemir-Urkmez, A., Hames, E.E.: Optimization of bacterial cellulose production by Gluconacetobacter xylinus using carob and haricot bean. Int. J. Biol. Macromol. 90, 2–10 (2016)

    Article  Google Scholar 

  11. Hong, F., Guo, X., Zhang, S., Han, S.-F., Yang, G., Jönsson, L.J.: Bacterial cellulose production from cotton-based waste textiles: enzymatic saccharification enhanced by ionic liquid pretreatment. Bioresour. Technol. 104, 503–508 (2012)

    Article  Google Scholar 

  12. Chen, L., Hong, F., Yang, X.-X., Han, S.-F.: Biotransformation of wheat straw to bacterial cellulose and its mechanism. Biores. Technol. 135, 464–468 (2013)

    Article  Google Scholar 

  13. Cheng, Z., Yang, R., Liu, X.: Production of bacterial cellulose by Acetobacter xylinum through utilizing acetic acid hydrolysate of bagasse as low-cost carbon source. BioResources 12(1), 1190–1200 (2017)

    Google Scholar 

  14. Stepanov, N., Efremenko, E.: “Deceived” concentrated immobilized cells as biocatalyst for intensive bacterial cellulose production from various sources. Catalysts 8(1), 33 (2018)

    Article  Google Scholar 

  15. Lu, H., Jia, Q., Chen, L., Zhang, L.: Effect of organic acids on bacterial cellulose produced by Acetobacter xylinum. J. Microbiol. Biotechnol. 5(2), 1–6 (2016)

    Google Scholar 

  16. Morgan, J.L., McNamara, J.T., Zimmer, J.: Mechanism of activation of bacterial cellulose synthase by cyclic di-GMP. Nat. Struct. Mol. Biol. 21(5), 489–496 (2014)

    Article  Google Scholar 

  17. Srivastava, D., Waters, C.M.: A tangled web: regulatory connections between quorum sensing and cyclic di-GMP. J. Bacteriol. 194(17), 4485–4493 (2012)

    Article  Google Scholar 

  18. Nuanpeng, S., Thanonkeo, S., Klanrit, P., Thanonkeo, P.: Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices. Braz. J. Microbiol. 49, 140–150 (2018)

    Article  Google Scholar 

  19. Cheng, K.-C., Catchmark, J.M., Demirci, A.: Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis. J. Biol. Eng. 3(1), 12 (2009)

    Article  Google Scholar 

  20. Basak, B., Bhunia, B., Dey, A.: Studies on the potential use of sugarcane bagasse as carrier matrix for immobilization of Candida tropicalis PHB5 for phenol biodegradation. Int. Biodeterior. Biodegrad. 93, 107–117 (2014)

    Article  Google Scholar 

  21. Liu, J., Chen, S., Ding, J., Xiao, Y., Han, H., Zhong, G.: Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its use in bioremediation of mesotrione-contaminated soils. Appl. Microbiol. Biotechnol. 99(24), 10839–10851 (2015)

    Article  Google Scholar 

  22. Yu, J., Zhang, X., Tan, T.: An novel immobilization method of Saccharomyces cerevisiae to sorghum bagasse for ethanol production. J. Biotechnol. 129(3), 415–420 (2007)

    Article  Google Scholar 

  23. Banerjee, A., Ghoshal, A.K.: Phenol degradation performance by isolated Bacillus cereus immobilized in alginate. Int. Biodeterior. Biodegrad. 65(7), 1052–1060 (2011)

    Article  Google Scholar 

  24. Abdelraof, M., Elsoud, M.M.A., Selim, M.H., Hassabo, A.A.: l-arginine amidinohydrolase by a new Streptomyces isolate: Screening and statistical optimized production using response surface methodology. Biocatal. Agric. Biotechnol. 24, 101538 (2020)

    Article  Google Scholar 

  25. Miller, G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31(3), 426–428 (1959)

    Article  Google Scholar 

  26. Santos, D.T., Sarrouh, B.F., Rivaldi, J.D., Converti, A., Silva, S.S.: Use of sugarcane bagasse as biomaterial for cell immobilization for xylitol production. J. Food Eng. 86(4), 542–548 (2008)

    Article  Google Scholar 

  27. Abdelraof, M., Selim, M.H., Elsoud, M.M.A., Ali, M.M.: Statistically optimized production of extracellular l-methionine γ-lyase by Streptomyces sp. DMMMH60 and evaluation of purified enzyme in sub-culturing cell lines. Biocatal. Agric. Biotechnol. 18, 101074 (2019)

    Article  Google Scholar 

  28. Lin, D., Lopez-Sanchez, P., Li, R., Li, Z.: Production of bacterial cellulose by Gluconacetobacter hansenii CGMCC 3917 using only waste beer yeast as nutrient source. Bioresour. Technol. 151, 113–119 (2014)

    Article  Google Scholar 

  29. Moreno Rivas, S.C., Armenta Corral, R.I., Frasquillo Félix, M.D.C., Islas Rubio, A.R., Vázquez Moreno, L., Ramos-Clamont Montfort, G.: Removal of cadmium from aqueous solutions by Saccharomyces cerevisiae–alginate system. Materials 12(24), 4128 (2019)

    Article  Google Scholar 

  30. Dzionek, A., Wojcieszyńska, D., Guzik, U.: Natural carriers in bioremediation: a review. Electron. J. Biotechnol. 19(5), 28–36 (2016)

    Article  Google Scholar 

  31. Dursun, A.Y., Tepe, O.: Internal mass transfer effect on biodegradation of phenol by Ca-alginate immobilized Ralstonia eutropha. J. Hazard. Mater. 126(1–3), 105–111 (2005)

    Article  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the National Research Center, Egypt for financial support. Since the research experiments was completely sponsored by Department of Microbial Chemistry Dep., and Paper and Cellulose Dep., National Research Centre, Giza, Egypt.

Funding

All expenses whether chemical analysis or molecular identification etc. were funded by ourselves and there is no funder or funding agency support us to finish this work.

Author information

Authors and Affiliations

  1. Microbial Chemistry Department, National Research Centre, Dokki, 12622, Cairo, Egypt

    Mohamed Abdelraof

  2. Cellulose and Paper Department, National Research Centre, Dokki, 12622, Cairo, Egypt

    Houssni El Saied & Mohamed S. Hasanin

Authors
  1. Mohamed Abdelraof
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Houssni El Saied
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohamed S. Hasanin
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MA carries out the immobilization process of the bacterial cells onto biopolymers, statistical optimization of the BC production and applies the reusability and storage stability test. MH makes the characterization of the cellulose-producing from each of system by FT-IR, XRD and SEM. MA, MH, and HE wrote the manuscript and participated in the data discussion, data analyses, and drafting of the manuscript. All authors have read and approved the manuscript.

Corresponding authors

Correspondence to Mohamed Abdelraof or Mohamed S. Hasanin.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interests.

Research Involving Human Participants and/or Animals

Not applicable.

Informed Consent

Not applicable.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Abdelraof, M., El Saied, H. & Hasanin, M.S. Green Immobilization of Glucanobacter xylinum onto Natural Polymers to Sustainable Bacterial Cellulose Production. Waste Biomass Valor 13, 2053–2069 (2022). https://doi.org/10.1007/s12649-021-01666-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12649-021-01666-w

Keywords

Search

Navigation