Skip to main content

Advertisement

SpringerLink
Log in

Exploration of Antidiabetic, Cholesterol-Lowering, and Anticancer Upshot of Probiotic Bacterium Pediococcus pentosaceus OBK05 Strain of Buttermilk

  • Published:
Probiotics and Antimicrobial Proteins Aims and scope Submit manuscript

Abstract

Chronic metabolic disorders such as hyperglycemia (diabetes), hyperlipidemia (high cholesterol), and cancer have become catastrophic diseases worldwide. Accordingly, probiotic intervention is a new approach for alleviating catastrophic diseases. In the present study, Pediococcus pentosaceus OBK05 was investigated as a potential probiotic bacterium for antidiabetic, cholesterol-lowering, and anticancer activities by in vitro and in vivo studies. Cell-free supernatant (CFS) of OBK05 showed potent antidiabetic activity by inhibition of α-amylase (72 ± 0.9%) and α-glucosidase (61 ± 0.8%) activity in vitro when compared to that of acarbose as standard (86 ± 0.9%). Furthermore, the in vivo cholesterol-lowering activity of OBK05 was studied using cholesterol-fed hypercholesterolemic mice. When compared to the cholesterol-fed group, the OBK05-intervened cholesterol-fed mice group had significantly lowered the levels of lipids and showed significantly lower lipid droplet accumulation in the histology of hepatocytes. Similarly, CFS exhibited higher anticancer activity (87.57 ± 1.27%) against HT-29 cells with an IC50 of 54.51 ± 1.8. Fluorescence-activated cell sorting (FACS) analysis revealed that CFS induced the cell cycle arrest by inhibiting the G1 to S phase transition. Further, in vivo anticancer activities were confirmed in BALB/c mice models, which were divided into four groups and held for 16 weeks. HT-29 cells (5×109/mice) were injected subcutaneously twice (0 and 2nd week), and OBK05 (3 ×105 CFU/mL) was given orally to the respective groups. The OBK05-intervened HT-29 cell–induced mice group showed significant improvement at the histological level and alleviated the degree of atypia in the intestine.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Data Availability

All data generated or analyzed during this study are included in this published research article. The supporting raw data of this article will be made available by the authors without undue reservation.

References

  1. Won G, Choi SI, Park N, Kim JE, Kang CH, Kim GH (2021) In vitro antidiabetic, antioxidant activity, and probiotic activities of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei strains. Curr Microbiol 78:3181–3191

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Son SH, Jeon HL, Yang SJ, Lee NK, Paik HD (2017) In vitro characterization of Lactobacillus brevis KU15006, an isolate from kimchi, reveals anti-adhesion activity against foodborne pathogens and antidiabetic properties. Microb Pathog 112:135–141

    Article  CAS  PubMed  Google Scholar 

  3. Yadav R, Dey DK, Vij R, Meena S, Kapila R, Kapila S (2018) Evaluation of anti-diabetic attributes of Lactobacillus rhamnosus MTCC: 5957, Lactobacillus rhamnosus MTCC: 5897 and Lactobacillus fermentum MTCC: 5898 in streptozotocin induced diabetic rats. Microb Pathog 125:454–462

    Article  CAS  PubMed  Google Scholar 

  4. Zhang Y, Wu T, Li W, Zhao Y, Long H, Liu R, Zhang M (2021) Lactobacillus casei LC89 exerts antidiabetic effects through regulating hepatic glucagon response and gut microbiota in type 2 diabetic mice. Food Funct 12:8288–8299

    Article  CAS  PubMed  Google Scholar 

  5. Wang G, Si Q, Yang S, Jiao T, Zhu H, Tian P, Wang L, Li X, Gong L, Zhao J, Zhang H, Chen W (2020) Lactic acid bacteria reduce diabetes symptoms in mice by alleviating gut microbiota dysbiosis and inflammation in different manner. Food Funct 11:5898–5914

    Article  CAS  PubMed  Google Scholar 

  6. Gérard C, Vidal H (2019) Impact of gut microbiota on host glycemic control. Front Endocrinol 29

  7. Lee S, Kim M (2019) Leuconostoc mesenteroides MKSR isolated from kimchi possesses α- glucosidase inhibitory activity, antioxidant activity, and cholesterol lowering effects. LWT 116:108570

    Article  CAS  Google Scholar 

  8. Kim S, Hong SP, Lim SD (2021) Physiological characteristics and anti-diabetic effect of Pediococcus pentosaceus KI62. Food Sci Anim Resour 41:274

    Article  PubMed  PubMed Central  Google Scholar 

  9. Wang G, Huang W, Xia Y, Xiong Z, Ai L (2019) Cholesterol-lowering potentials of Lactobacillus strains overexpression of bile salt hydrolase on high cholesterol diet-induced hypercholesterolemic mice. Food funct 10:1684–1695

    Article  CAS  PubMed  Google Scholar 

  10. World Health Organization (WHO) (2020) https://www.who.int/en/news-room/factsheets/detail/cardiovascular-diseases-(cvds). Accessed 23 Feb 2020

  11. Lim PS, Loke CF, Ho YW, Tan HY (2020) Cholesterol homeostasis associated with probiotic supplementation in vivo. J Appl Microbial 129:1374–1388

    Article  CAS  Google Scholar 

  12. Ding Z, Hani A, Li W, Ke W, Guo X (2020) Influence of a cholesterol-lowering strain Lactobacillus plantarum LP3 isolated from traditional fermented yak milk on gut bacterial microbiota and metabolome of rats fed with a high-fat diet. Food Funct 11:8342–8353

    Article  CAS  PubMed  Google Scholar 

  13. Guan X, Xu Q, Zheng Y, Qian L, Lin B (2017) Screening and characterization of lactic acid bacterial strains that produce fermented milk and reduce cholesterol levels. Braz J Microbiol 48:730–739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Saikia D, Manhar AK, Deka B, Roy R, Gupta K, Namsa ND, Chattopadhyay P, Doley R, Mandal M (2018) Hypocholesterolemic activity of indigenous probiotic isolate Saccharomyces cerevisiae ARDMC1 in a rat model. J Food Drug Anal 26:154–162

    Article  CAS  PubMed  Google Scholar 

  15. Shao Y, Huo D, Peng Q, Pan Y, Jiang S, Liu B, Zhang J (2017) Lactobacillus plantarum HNU082-derived improvements in the intestinal microbiome prevent the development of hyperlipidaemia. Food funct 8:4508–4516

    Article  CAS  PubMed  Google Scholar 

  16. Ding W, Shi C, Chen M, Zhou J, Long R, Guo X (2017) Screening for lactic acid bacteria in traditional fermented Tibetan yak milk and evaluating their probiotic and cholesterol- lowering potentials in rats fed a high-cholesterol diet. J Funct Foods 32:324–332

    Article  CAS  Google Scholar 

  17. Damodharan K, Palaniyandi SA, Y SH, Suh JW, (2016) Functional probiotic characterization and in vivo cholesterol-lowering activity of Lactobacillus helveticus isolated from fermented cow milk. J Microbiol Biotechnol 26:1675–1686

    Article  CAS  PubMed  Google Scholar 

  18. Mann GV, Spoerry A (1974) Studies of a surfactant and cholesteremia in the Maasai. AM J Clin Nutr 27:464–469

    Article  CAS  PubMed  Google Scholar 

  19. Bedada TL, Feto TK, Awoke KS, Garedew AD, Yifat AT, Birri DJ (2020) Probiotics for cancer alternative prevention and treatment. Biomed pharmacother 129:110409

    Article  Google Scholar 

  20. Nazir Y, Hussain SA, Abdul Hamid A, Song Y (2018) Probiotics and their potential preventive and therapeutic role for cancer, high serum cholesterol, and allergic and HIV diseases. Biomed Res Int. https://doi.org/10.1155/2018/3428437

    Article  PubMed  PubMed Central  Google Scholar 

  21. O’Toole PW, Marchesi JR, Hill C (2017) Next-generation probiotics: the spectrum from probiotics to live biotherapeutics. Nat Microbial 2:1–6

    Google Scholar 

  22. Xiao L, Ge X, Yang L, Chen X, Xu Q, Rui X, Fan X, Feng L, Zhang Q, Dong M, Li W (2020) Anticancer potential of exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cell via apoptosis induction. Food Funct 11:10170–10181

    Article  CAS  PubMed  Google Scholar 

  23. Taieb J, André T, Auclin E (2019) Refining adjuvant therapy for non-metastatic colon cancer, new standards and perspectives. Cancer Treat Rev 75:1–11

    Article  CAS  PubMed  Google Scholar 

  24. Tiptiri-Kourpeti A, Spyridopoulou K, Santarmaki V, Aindelis G, Tompoulidou E, Lamprianidou EE, Saxami G, Psilantis PY, Lampri ES, Simopoulos C, Kotsianidis I (2016) Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells. PLoS One 11:e0147960

    Article  PubMed  PubMed Central  Google Scholar 

  25. Bhukya KK, Bhukya B (2021) Unraveling the probiotic efficiency of bacterium Pediococcus pentosaceus OBK05 isolated from buttermilk: an in vitro study for cholesterol assimilation potential and antibiotic resistance status. PLoS One 16:e0259702

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Wang G, Li X, Zhao J, Zhang H, Chen W (2017) Lactobacillus casei CCFM419 attenuates type 2 diabetes via a gut microbiota dependent mechanism. Food Funct 8:3155–3164

    Article  CAS  PubMed  Google Scholar 

  27. Pammi N, Bhukya KK, Lunavath RK, Bhukya B (2021) Bioprospecting of Palmyra palm (Borassus flabellifer) nectar: unveiling the probiotic and therapeutic potential of the traditional rural drink. Front Microbiol 12

  28. AlKalbani NS, Turner MS, Ayyash MM (2019) Isolation, identification, and potential probiotic characterization of isolated lactic acid bacteria and in vitro investigation of the cytotoxicity, antioxidant, and antidiabetic activities in fermented sausage. Microb Cell Fact 18:1–12

    Article  CAS  Google Scholar 

  29. Jafari-Nasab T, Khaleghi M, Farsinejad A, Khorrami S (2021) Probiotic potential and anticancer properties of Pediococcus sp. isolated from traditional dairy products. Biotechnol Rep 29:e00593

  30. Chandel D, Sharma M, Chawla V, Sachdeva N, Shukla G (2019) Isolation, characterization and identification of antigenotoxic and anticancerous indigenous probiotics and their prophylactic potential in experimental colon carcinogenesis. Sci Rep 9:1–12

    Article  CAS  Google Scholar 

  31. Rajoka MSR, Zhao H, Lu Y, Lian Z, Li N, Hussain N, Shi J (2018) Anticancer potential against cervix cancer (HeLa) cell line of probiotic Lactobacillus casei and Lactobacillus paracasei strains isolated from human breast milk. Food Funct 9:2705–2715

    Article  Google Scholar 

  32. Dubey V, Ghosh AR, Bishayee K, Khuda-Bukhsh AR (2016) Appraisal of the anti-cancer potential of probiotic Pediococcus pentosaceus GS4 against colon cancer: in vitro and in vivo approaches. J Funct Foods 23:66–79

    Article  CAS  Google Scholar 

  33. Asadollahi P, Ghanavati R, Rohani M, Razavi S, Esghaei M, Talebi M (2020) Anticancer effects of Bifidobacterium species in colon cancer cells and a mouse model of carcinogenesis. PLoS One 15(5):e0232930

    Article  PubMed  Google Scholar 

  34. Wang L, Shang Q, Guo W, Wu X, Wu L, Wu L, Chen T (2020) Evaluation of the hypoglycemic effect of probiotics via directly consuming glucose in intestines of STZ-induced diabetic mice and glucose water-induced diabetic mice. J Funct Foods 64:103614

    Article  CAS  Google Scholar 

  35. Li X, Wang N, Yin B, Fang D, Zhao J, Zhang H, Wang G, Chen W (2016) Lactobacillus plantarum X1 with α-glucosidase inhibitory activity ameliorates type 2 diabetes in mice. RSC advances 6:63536–63547

    Article  CAS  Google Scholar 

  36. Koh WY, Utra U, Ahmad R, Rather IA, Park YH (2018) Evaluation of probiotic potential and anti-hyperglycemic properties of a novel Lactobacillus strain isolated from water kefir grains. Food Sci Biotechnol 27:1369–1376

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Cai T, Wu H, Qin J, Qiao J, Yang Y, Wu Y, Qiao D, Xu H, Cao Y (2019) In vitro evaluation by PCA and AHP of potential antidiabetic properties of lactic acid bacteria isolated from traditional fermented food. LWT 115:108455

    Article  CAS  Google Scholar 

  38. Kang JH, Yun SI, Park HO (2010) Effects of Lactobacillus gasseri BNR17 on body weight and adipose tissue mass in diet-induced overweight rats. J Microbiol 48:712–714

    Article  PubMed  Google Scholar 

  39. Damodharan K, Lee YS, Palaniyandi SA, Yang SH, Suh JW (2015) Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7and in vivo assessment of its cholesterol-lowering activity. Front Microbial 6:768

    Google Scholar 

  40. Fazeli H, Moshtaghian J, Mirlohi M, Shirzad M (2010) Reduction in serum lipid parameters by incorporation of a native strain of Lactobacillus Plantarum, A7 in Mice. J Diabetes Metab Disord 9:22

    Google Scholar 

  41. Wu Y, Zhang Q, Ren Y, Ruan Z (2017) Effect of probiotic Lactobacillus on lipid profile: A systematic review and meta-analysis of randomized, controlled trials. PLoS One 12:e0178868

    Article  PubMed  PubMed Central  Google Scholar 

  42. Ye H, Li Q, Zhang Z, Sun M, Zhao C, Zhang T (2017) Effect of a novel potential probiotic Lactobacillus paracasei, Jlus66 isolated from fermented milk on nonalcoholic fatty liver in rats. Food Funct 8:4539–4546

    Article  CAS  PubMed  Google Scholar 

  43. El-Deeb NM, Yassin AM, Al-Madboly LA, El-Hawiet A (2018) A novel purified Lactobacillus acidophilus 20079 exopolysaccharide, LA-EPS-20079, molecularly regulates both apoptotic and NF-κB inflammatory pathways in human colon cancer. Microb Cell Fact 17:1–15

    Article  Google Scholar 

  44. Jaskulski IB, Uecker J, Bordini F, Moura F, Gonçalves T, Chaves NG, Camargo F, Grecco FB, Fiorentini AM, da Silva WP, Andreazza R (2020) In vivo action of Lactococcus lactis subsp. lactis isolate (R7) with probiotic potential in the stabilization of cancer cells in the colorectal epithelium. Process Biochem 91:165–171

    Article  CAS  Google Scholar 

  45. Lenoir M, Del Carmen S, Cortes-Perez NG, Lozano-Ojalvo D, Muñoz-Provencio D, Chain F, Langella P, de Moreno de LeBlanc A, LeBlanc JG, Bermúdez-Humarán LG, (2016) Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer. J gastroenterol 51:862–873

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

Authors are grateful to the University Grants Commission (UGC), Govt. of India, Indian Council of Medical Research, Govt. of India and the Ministry of Human Resource Development, Govt. of India, for financial support to carry out the present study.

Funding

This work was supported by the University Grants Commission (UGC), Govt. of India, through major research project (F. No. 41- 1145/2012(SR)) and RGNF fellowship (F. No. 2015-17/ RGNF-2015-17-TEL-1236) and the Ministry of Human Resource Development, Govt. of India, through RUSA 2.0 program.

Author information

Authors and Affiliations

  1. Centre for Microbial and Fermentation Technology, Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India

    Kiran Kumar Bhukya & Bhima Bhukya

Authors
  1. Kiran Kumar Bhukya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bhima Bhukya
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Kiran Kumar Bhukya designed and carried out all the experiments pertaining to microbial screening, strain isolation, in vitro probiotic characteristics, in vivo experiments and prepared the manuscript. Bhima Bhukya conceptualized and monitored the experiments, corrected the manuscript and the corresponding author. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Bhima Bhukya.

Ethics declarations

Disclaimer

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflict of Interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bhukya, K.K., Bhukya, B. Exploration of Antidiabetic, Cholesterol-Lowering, and Anticancer Upshot of Probiotic Bacterium Pediococcus pentosaceus OBK05 Strain of Buttermilk. Probiotics & Antimicro. Prot. 15, 1484–1500 (2023). https://doi.org/10.1007/s12602-022-10002-0

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12602-022-10002-0

Keywords

Search

Navigation