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Effect of Lactobacillus plantarum Tennozu-SU2 on Salmonella Typhimurium Infection in Human Enterocyte-Like HT-29-Luc Cells and BALB/c Mice

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Abstract

The probiotic properties and inhibitory effect on Salmonella Typhimurium adhesion on human enterocyte-like HT-29-Luc cells of three Lactobacillus plantarum strains isolated from fermented fish, beach sand and a coastal plant were determined. Compared with the type strain L. plantarum NBRC 15891T, which was isolated from pickled cabbage, L. plantarum Tennozu-SU2 isolated from the acorn of a coastal tree showed high autoaggregation in de Man, Rogosa and Sharpe (MRS) broth and an antagonistic effect against S. Typhimurium in brain heart infusion (BHI) broth. Furthermore, heat-killed L. plantarum Tennozu-SU2 cells inhibited S. Typhimurium adhesion on HT-29-Luc cells. Both live and heat-killed L. plantarum Tennozu-SU2 cells showed an inhibitory effect on gut colonisation in BALB/c mice, as assessed by viable Salmonella count in faecal samples and by invasion into liver and spleen tissues. The properties shown in this study suggest that L. plantarum Tennozu-SU2 is useful as a starter and probiotic bacteria in functional food material.

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Acknowledgements

The present study was supported in part by the Terrada Warehouse Company, Tokyo, Japan.

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan

    Shino Hirano, Yasushi Yokota, Mika Eda, Takashi Kuda, Ayane Shikano, Hajime Takahashi & Bon Kimura

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  1. Shino Hirano
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  2. Yasushi Yokota
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  4. Takashi Kuda
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Correspondence to Takashi Kuda.

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The animal experiments were performed in compliance with the Fundamental Guidelines for Proper Conduct of Animal Experiment and Related Activities in Academic Research Institutions, under the jurisdiction of the Ministry of Education, Culture, Sports, Science and Technology, and approved by the animal experiment committee of Tokyo University of Marine Science and Technology (approval no. H28-4).

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The authors declare that they have no conflict of interest.

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Hirano, S., Yokota, Y., Eda, M. et al. Effect of Lactobacillus plantarum Tennozu-SU2 on Salmonella Typhimurium Infection in Human Enterocyte-Like HT-29-Luc Cells and BALB/c Mice. Probiotics & Antimicro. Prot. 9, 64–70 (2017). https://doi.org/10.1007/s12602-016-9243-9

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