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Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA

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Abstract

This study aimed at characterizing the distribution of black sea bream Acanthopagrus schlegelii, a major target fish of commercial and recreational fishing, using environmental DNA (eDNA). Twelve survey stations were aligned along an inshore-offshore axis in Tango Bay, southwestern Japan. Water samples were taken from the surface and bottom layers at each station monthly from May 2017 through April 2018 (twice in June 2017 and excluding October 2017). Quantitative polymerase chain reaction (qPCR) with species-specific primers and probe was used to detect DNA of black sea bream in water samples. Throughout the year, eDNA was abundant at inshore stations, especially at stations close to the river mouth in the innermost part of the bay. In May and June, eDNA was detected even at the most offshore station 19 km from the coast, whereas eDNA was detected exclusively within 3 km from the coastline during the months of July through April. The eDNA detected offshore in May and June were likely to have originated from eggs and/or larvae, as this species spawns pelagic eggs in early summer. These results through the use of eDNA indicated that black sea bream is highly dependent on coastal areas in its life history.

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Acknowledgements

We would like to thank Capt. Yoshihito Ogura for operating R/V Ryokuyo maru and also for providing advice for surveys, Mizuki Ogata, Misaki Shiomi in MFRS and Dr. Toshiaki Jo at Ryukoku University for assistance with field surveys and water filtration. We are grateful to Dr. Daiki Tomojiri at Kyoto University for his statistical advice. We also thank the staff and students in MFRS for constructive comments throughout the research. Hunter Godfrey from Santa Barbara, California, kindly proofread the earlier version of the manuscript. Comments from two anonymous reviewers substantially improved the quality of the manuscript. This work was supported by JST CREST Grant Number JPMJCR13A2, Japan and JSPS KAKENHI Grant Number JP19H03031 and JP19H05641.

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Authors and Affiliations

  1. Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University, Nagahama, Maizuru, Kyoto, 625-0086, Japan

    Sachia Sasano, Hiroaki Murakami, Keita W. Suzuki, Yoh Yamashita & Reiji Masuda

  2. Center for the Promotion of Interdisciplinary Education and Research, Kyoto University, Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto, Kyoto, 606-8502, Japan

    Hiroaki Murakami & Yoh Yamashita

  3. Graduate School of Human Development and Environment, Kobe University, Tsurukabuto, Nada, Kobe, Hyogo, 657-8501, Japan

    Toshifumi Minamoto

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  1. Sachia Sasano
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Correspondence to Sachia Sasano.

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Sasano, S., Murakami, H., Suzuki, K.W. et al. Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA. Fish Sci 88, 91–107 (2022). https://doi.org/10.1007/s12562-021-01572-z

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