Improvement of the Bag-Mediated Filtration System for Sampling Wastewater and Wastewater-Impacted Waters

Environmental surveillance of poliovirus (PV) plays an important role in the global program for eradication of wild PV. The bag-mediated filtration system (BMFS) was first developed in 2014 and enhances PV surveillance when compared to the two-phase grab method currently recommended by the World Health Organization (WHO). In this study, the BMFS design was improved and tested for its usability in wastewater and wastewater-impacted surface waters in Nairobi, Kenya. Modifications made to the BMFS included the size, color, and shape of the collection bags, the filter housing used, and the device used to elute the samples from the filters. The modified BMFS concentrated 3–10 L down to 10 mL, which resulted in an effective volume assayed (900–3000 mL) that was 6–20 times greater than the effective volume assayed for samples processed by the WHO algorithm (150 mL). The system developed allows for sampling and in-field virus concentration, followed by transportation of the filter for further analysis with simpler logistics than the current methods. This may ultimately reduce the likelihood of false-negative samples by increasing the effective volume assayed compared to samples processed by the WHO algorithm, making the BMFS a valuable sampling system for wastewater and wastewater-impacted surface waters.


Introduction
Environmental surveillance is crucial for monitoring and preventing the spread of pathogens, which are present in wastewater, surface waters impacted by wastewater, recreational waters, and drinking water sources (Betancourt et al. 2014;Deboosere et al. 2011;Fuhrimann et al. 2015) that humans and animals regularly contact. Environmental surveillance can highlight epidemiological patterns, especially for diseases with high subclinical infection rates, where pathogens silently circulate. For example, although less than 1 in 100 poliovirus infections results in acute flaccid paralysis, the virus is shed in the stool of all infected persons (Nathanson and Kew 2010). Environmental surveillance of wastewater can detect poliovirus from these asymptomatic carriers, resulting in early pathogen detection, focused vaccine efforts, and eventual eradication certification (Hovi et al. 2012).
As pathogens are typically present in low numbers in wastewater-impacted surface waters, detection can be challenging. The likelihood of detection can be improved through concentration of the environmental samples, which results in an increase in the effective volume assayed. Typically, after grab sample collection, concentration occurs in the laboratory by chemical and/or physical mechanisms. For example, when sampling for poliovirus from wastewater and wastewater-impacted surface waters, the two-phase grab sample method commonly used by the World Health Organization (WHO) involves concentrating 500 mL down to 10 mL by a two-phase separation method using polyethylene glycol (PEG) and dextran (World Health Organization 2015). For physical concentration, vacuum-driven or pump-driven filtration is often used (Gantzer et al. 1997;Karim et al. 2009;Katayama et al. 2002). These types of filtration methods require concentration to be completed in the laboratory, which requires transportation and storage of samples prior to processing. In low-resource settings, such sampling methodology can be expensive and logistically challenging to perform.
On-site filtration could be used to concentrate large sample volumes, resulting in a high effective volume assayed and an increased likelihood of pathogen detection. Filtration at the field site is advantageous because it reduces the need to transport large volumes of potentially biohazardous materials, allows for sampling of larger volumes without requiring transportation or shipment of heavy samples, and reduces the storage footprint in spacelimited laboratories. However, in-field filtration typically requires the use of an electric-powered pump (Corsi et al. 2014). This can make filtration in the field prohibitive as pumps are expensive, require a continual power source, and are large and heavy. Portable, low-cost considerations for in-field filtration are needed.
The objective of this study was to improve the usability and sensitivity of the previously developed bag-mediated filtration system (BMFS) (Fig. 1) (Fagnant et al. 2014). This system enables in-field filtration of samples while requiring no power. It is compact enough to fit inside of a backpack and is simple to use. In previous tests, the BMFS could filter more than 10 L of effluent from primary clarifiers at a local wastewater treatment plant and 3-5 L of influent wastewater (Fagnant et al. 2014), which increased the virus concentration compared to the recommended twophase sampling method by the WHO for poliovirus detection. Filtration of wastewater and wastewater-impacted surface waters in Nairobi, Kenya demonstrated the usability of the BMFS, and deployment feedback-guided modifications to the BMFS design. This paper describes those modifications.

BMFS Design
The basic BMFS consists of a 6-to 12-L collection bag, filter, collapsible tripod stand, personal protective equipment, and decontamination supplies. All supplies are transportable in an insulated backpack.
Sample collection bags underwent three design iterations ( Fig. 1; Table 1). First-generation collection bags held 10-L, bright yellow polyurethane-coated nylon sock bags with 1-cm heat-sealed seams (Fig. 1a). The material was flexible, durable, and water-tight. Samples were collected at the top, which was held open by a detachable brass ring that was inserted through holes at the bag's top opening. A rope was attached to the ring for sample a First-generation yellow, 10-L bag. Bag outlet was located 4 cm above the bag's bottom. b Second-generation drab green, 10-L bag. Bag outlet was located 10 cm above the bag's bottom. 0.5-L volume increments were demarcated on the bag. c Third-generation drab green, 6-L bag. Bag outlet was located at the bag's bottom, and a 15.5°slope was heat sealed onto the bottom of the bag. 0.25-L volume increments were demarcated on the bag Food Environ Virol (2018) 10:72-82 73 collection. Samples flowed through a quick-disconnect check valve port located 4 cm above the bag's bottom, to which the filter was attached after collection. Reinforced holes on the bag's side seam allowed it to hang on the tripod stand (Fagnant et al. 2014). Second-generation collection bags held 10 L, were drab green with white screen printing that demarcated 0.5-L volume increments, and had 1-cm heat-sealed seams (Fig. 1b). Samples were collected at the top, which was held open by a stainless steel rolled ring clamp with a spring form mechanism. The inner and outer rings were attached by a lanyard. The clamp held a pre-screen mesh on the inlet of the bag, and the mesh had a uniform pore size. Several different mesh pore sizes were tested, ranging Typically, field technicians processed less than 4-L wastewater or wastewaterimpacted surface waters in Nairobi, making collection of a 10-L water sample unnecessary. A 6-L bag enabled bleeding and collection of an initial 1 L settled volume, and filtration of the remaining clarified 5 L A 12-L bag facilitated use of a new protocol (not discussed in this work). The large bag enabled bleeding and collection of an initial 2 L settled volume, and filtration of the remaining clarified 10 L through two filters Bright yellow bags attracted attention from on-lookers. Therefore, the color was changed to drab green to reduce attention Volumetric demarcations were added, enabling measurement of the filtered volume 3 Drab green with white screen printing Volumetric demarcations: 0.25-L increments The volumetric demarcations were changed to 0.25-L gradations to facilitate easier and more accurate measurement of the filtered volume from 249 lm to 1.2 mm, and a final pore size of 249 lm was selected. The clamp also included a loop for rope attachment and an angled loop for easy placement on the tripod stand. Samples flowed through an open -inch-diameter barbed tubing adapter port located 10 cm above the bag's bottom. An 8-cm section of tubing with a tubing clamp was attached to the port, which was clamped while the bag was filling. Third-generation collection bags held 6 or 12 L, were drab green with white screen printing that demarcated 0.25-L volume increments, and had 1-cm heat-sealed seams (Fig. 1c). Sample collection was completed the same as for the second-generation bag with the clamp and pre-screen mesh. The port was moved to the bottom of the bag and a 15.5°slope was heat sealed onto the bottom of the bag to angle sediments toward the outlet port so they could be collected prior to filtration.

Laboratory Testing of Filters
For laboratory testing, 10 L influent wastewater (after bar screens) grab samples were collected from the West Point Wastewater Treatment Plant in Seattle, WA, USA. Samples were collected by bucket and rope, poured into 20-L carboys, and transported back to the laboratory where they were stored at room temperature until processing (within 7 days of collection). Samples were subsequently filtered through 5-cm ViroCap TM filters (Scientific Methods, Inc., Granger, IN, USA) using a peristaltic pump.

Field Testing of BMFS
For field testing, 10 L surface water grab samples were collected from Lake Union in Seattle, WA, USA and from four study sites in Nairobi, Kenya. In Nairobi, samples were collected from the Motoine River (which is impacted by untreated latrine waste) bordering the Kibera informal settlement, a latrine waste stream in the Mathare informal settlement that flows to the Mathare River, and sewer conveyance lines in the Eastleigh neighborhood (two sites). These collection sites will be referred to as Kibera, Mathare, Eastleigh A, and Eastleigh B.
For sample collection, the BMFS collection bag was lowered into the source by the rope and filled, then hauled up and hung on the tripod. The sample settled for 15 min, and then the settled solids and debris were drained until 1 L of the sample was drained or until a clarified liquid passed from the bag. Tubing and a cartridge filter were subsequently attached to the outlet port (Fig. 2). The tubing clamp was opened and the sample filtered under gravity, with a 0.5-m head for disposable filter housings and a 0.7-m head for reusable filter housings. When the gravity head was inadequate to drive filtration, the collection bag was sealed with a clamp, removed from the tripod stand, and rolled by hand to apply pressure and filter the remaining sample. Samples were filtered through 5-cm ViroCap filters up to 10 L or until clogging. Filters were returned to the laboratory under cold chain and processed.

ViroCap Filter Housings
Commercially produced, disposable ViroCap filter housings and reusable ViroCap filter housings designed for this study were used for laboratory tests and field sampling. The reusable housings aimed to reduce the system's environmental footprint and reduce the holdup/elution volume from 175 to 100 mL. The holdup volume is defined as the non-solid void within the filter housing, a measurement used in calculating the concentration factor of the system. The reusable housing sumps were custom-designed, injection-molded, made from clear polycarbonate (Protolabs, Maple Plain, MN, USA), and used in conjunction with a reinforced polypropylene lid (Pentek, Inc, Upper Saddle River, NJ, USA). The filter is seated in the body of the housing and the lid is tightened with the use of a

Elution and Secondary Concentration
ViroCap filters were eluted in a 1.5% beef extract and 0.05 M glycine solution, at pH 9.5. The eluent was poured into the filter housing inlet, let stand for a specified period of time, and then pumped out with a peristaltic pump. The eluate was adjusted to pH 7.0-7.5 with 5 M HCl and 5 M NaOH. Disposable housings were eluted with 175 mL and the eluent was held in the filter housing for 30 min. Reusable housings were eluted by two methods: (1) a single elution in which they were eluted with 100 mL and the eluent was held in the filter housing for 30 min, and (2) a double elution in which they were eluted two times with 100 mL and the eluent was held in the filter housing for 15 min for each elution. Secondary concentration was performed by PEG/NaCl precipitation. PEG 8000 (14 g/100 mL) and NaCl (1.17 g/ 100 mL) were added to the sample and dissolved by vigorous shaking (5 min). Samples were shaken overnight (200 RPM, 4°C) and then centrifuged (60009g, 4°C, 30 min). The supernatant was discarded and the pellet was resuspended in 10 mL of phosphate-buffered saline.

Elution Device
An elution apparatus was designed to eliminate the need for a peristaltic pump during elution (Fig. 3). A disposable syringe (Fig. 3a) injects 100 mL of eluent into the reusable filter housing. A vent was placed in the injection line to allow air in the injection line to escape, thus preventing the formation of air lock in the line and the filter housing (Fig. 3b). The eluate is held in the ViroCap filter ( Fig. 3c) for 30 min until it is eluted through the filter and transferred into a collection container (stool collection cup) with a modified lid (Fig. 3d). An Alfa Marine manual bilge pump provides suction for the sample elution (Fig. 3f) (Alfa Marine Co., Ltd, Shanghai, China). A Whatman Ò Vacu-Guard hydrophobic filter ( Fig. 3e; GE Healthcare Bio-Sciences, Pittsburgh, PA, USA) is placed on the collection cup outlet to prevent liquid from entering the pump.

Water Sample Characteristics
Concentrations of total dissolved solids (TDS) were determined for the water samples collected at the Kibera, Mathare, Eastleigh A, and Eastleigh B sites. TDS were determined by Kenya Standard Method 459 (Gazette 1985).

Filter Clogging Challenge
To determine particle sizes that resulted in filter clogging in the BMFS, 10 L of tap water seeded with 25 g soil particles was filtered through the BMFS in the laboratory. Particle sizes included \850, \180, \150, \63, and \25 lm. Filters were also tested for clogging by filtering 10 L of surface water and influent wastewater samples from Seattle, WA, USA. Field testing was conducted at the Kibera, Mathare, Eastleigh A, and Eastleigh B sites in Nairobi.

Poliovirus Culture and Recovery Experiments
The vaccine strain of poliovirus type 1 (PV1) stocks were prepared by confluent lysis of buffalo green monkey kidney (BGMK) cell monolayers (Sobsey and Jones 1979). PV1 was extracted using Vertrel XF (E. I. du Pont de Nemours and Company, Wilmington, DE, USA) and purified stocks were stored at -80°C (Mendez et al. 2000).
To determine the poliovirus recovery efficiency using the modified BMFS, laboratory-seeded experiments were completed. Influent wastewater samples were seeded with *10 PFU/mL PV1 prior to filtration through ViroCap filters in either disposable or reusable housings. Samples were then eluted using a single elution (175 mL) with the disposable housing, and either a single elution (100 mL) or a double elution (100 mL each) with the reusable housing. Samples were then concentrated to 10 mL by PEG/NaCl and analyzed for PV1 by plaque assay. Viruses were enumerated on 95% confluent BGMK cells as previously described and modified to include an Avicel RC-581 (FMC Corporation, Philadelphia, PA, USA) overlay (Sobsey and Jones 1979;Matrosovich et al. 2006). Assays were performed in triplicate onto 9.5 cm 2 wells using 200-lL aliquots of relevant dilutions. Infected cells were incubated at 37°C and 5% CO 2 for 40-44 h and then stained with 2% crystal violet in 20% methanol. Plaques were counted for infectious virus enumeration. Viral recovery was calculated by dividing the recovered viral count by the seeded viral count.

Statistical Analyses
The confidence intervals (CI) on sample volume and filtration time were calculated by where x is the mean, t is the critical value when the degrees of freedom is (n -1) and a is 0.05, r is the standard deviation, and n is the number of samples. Unpaired Student's or Welch's t tests were used to compare PV recoveries between filtration through a disposable versus reusable filter housing, while using a single or double elution.

Collection Bag Design
The design of the BMFS went through several iterations based on results from field tests concerning the filterability of the samples and other observations (Table 1; Fig. 1).
For the first generation of the BMFS, laboratory experiments suggested that collection and processing up to 10 L of primary or secondary wastewater samples was feasible when using the ViroCap filter (Fagnant et al. 2014). During field evaluations, however, high amounts of sediments clogged the system after filtration of 2-3 L of wastewater and wastewater-impacted surface waters from several sites in Nairobi. The clogging occurred in either the ViroCap filter (Kibera site) or collection bag (Eastleigh B site). It was thought that the clogging of the filter was due to fine suspended particles in the water samples. Clogging of the collection bag likely occurred inside of the outlet check valve due to an accumulation of celluloid material observed in the water, which prevented liquid from exiting the bag.
Based on these observations, the BMFS was modified to create a second-generation form (Table 1; Fig. 1b). Laboratory evaluation demonstrated that collection and filtration of 10 L of influent wastewater was possible with the ViroCap filters, though filter clogging issues remained during the field evaluation. Collection bag clogging was not encountered in the field because the check valve choke point was removed (Table 1). Water analyses at the four sites in Nairobi found that TDS concentrations were heavily loaded in Mathare and Eastleigh A, and moderately loaded in Kibera and Eastleigh B (Table 2). A visual examination of the sampling process at Kibera revealed visible particle loading of the 249-lm pre-screen mesh and a significant amount of fine sediments passing through the pre-screen. At all sites, the filters clogged after approximately 3-4 L of sample water was filtered. It is likely that filters were clogging during the field evaluation due to fine suspended particles or other constituents blocking the filter sites (e.g., fats, soap). This observation was supported by laboratory experiments, which found that fine particles (\25 lm) clogged the ViroCap filter more than twice as quickly as with larger particles (\63 lm). Filters loaded with particles \850, \180, \150, and \63 lm in size experienced no clogging issues for gravity filtration of 10 L of tap water with 2500 mg/L particle matter.
The third generation of the BMFS collection bag was modified based on these new observations (Table 1; Fig. 1c). This new design enabled a 15-min clarification/ settling period, followed by bleeding of the settled solids to a second vessel prior to filtration of the remaining clarified sample. Initial settling occurred while the user performed other tasks; typically, 15 min passed while the bag hung on the tripod stand. After settling, 0.5-1.0 L of settled sediments were collected in a Whirl-Pak Ò bag (Nasco, Fort Atkinson, WI, USA), and the clarified sample was filtered until the filter clogged. If the full clarified volume was filtered, the collected sediments were poured back into the sample collection bag for filtration.

Filter Design
Disposable ViroCap filter housings were used during the initial development of the BMFS. The filter design was modified based on observations from field tests that indicated slow filtration. Reusable housings were designed to reduce the system's environmental footprint and reduce the holdup volume from 175 to 100 mL. Reduction of the holdup/elution volume from 175 mL with the disposable housing to 100 mL with the reusable housing improves the likelihood of pathogen detection. This is due to an increase in the concentration factor from 1:57 for the disposable housing to 1:100 for the reusable housing when a 10-L sample is filtered. Therefore, if the eluate is assayed directly, the effective volume assayed and subsequent likelihood of detection is increased by 75% for the reusable housing over the disposable housing. A smaller eluate volume also reduces the processing time in the laboratory required for secondary concentration. Secondary concentration may be desirable to increase the effective volume assayed for viruses expected to be present in low concentration or for assays requiring low sample volumes.
The disposable and reusable ViroCap filter housings were evaluated for use with the BMFS and their performance was measured by comparing the time required for gravity filtration (passive) and by roll-expressed filtration (active) (Fig. 4). Similar times were required for active filtration of 2.5 to 4 L wastewater-impacted surface water when using the disposable housings and the reusable housings. By contrast, the disposable housings required 20 min longer on average during passive filtration. Passive filtration provided 4.8 kPa pressure on the system for the disposable housing and 6.9 kPa for the reusable housing. Active filtration exhibited an average of 27.6 kPa on the system and up to 44.8 kPa for both the disposable and reusable housings.
The extra time required for the disposable housing when using passive filtration is due to its shape (Fig. 5a) as compared to that of the reusable housing (Fig. 5b). For the disposable housing, the inlet and outlet are located on the top of the unit, and the outlet tubing has an inverted ''U'' shape where the fluid exits the system. This ''U'' shape reduces the hydrostatic head for gravitational flow by 0.2 m above the filter housing unit and therefore decreases the pressure available to drive filtration. Also, there is insufficient pressure to initially prime the system to allow flow, requiring users to manually wet out the system by ''milking,'' or squeezing the tubing and increasing the time required to filter the sample. By comparison, the inlet and outlet on the reusable housing are located on the sides of the filter housing lid, increasing the potential hydrostatic head compared to that seen in the disposable housing. The added pressure from this orientation is substantial enough to expel air from the housing and create a siphon effect with minimal user effort. Based on time to filter in the field, it was demonstrated that effective filtration is orientation independent and the reusable housing does not require a stand for use on uneven ground (data not shown).
After field evaluation, the reusable housing was yet further modified to improve its efficiency during the elution process. One major concern with the original design was the formation of air bubbles in the filter housing when the liquid level on the inner side of the filter was higher than the liquid level outside of the filter during the elution procedure (Fig. 6a). This foam was generated by the highvelocity air flow that was pumped through the elution device in order to collect the sample from the filter. The Total volume filtered ranged from 2.9 to 4.0 L. n = 2 for disposable housing, n = 6 for reusable housing. Error bars represent standard deviation foam persisted in the filter housing and the elution tubing after the elution procedure was finished, decreasing the recovered sample volume and potentially aerosolizing pathogens (Walls et al. 2014). To reduce foam formation during the elution procedure, the filter holder was modified by inserting an extension tube to the suction port (outlet) of the filter housing (Fig. 6b). This repositioning of the suction port near the bottom of the filter housing (Fig. 6b) enables collection of the liquid at the bottom, limiting the air entrainment into the liquid sample. In this case, the liquid level on the outer side of the filter element is always higher than that on the inner side of the filter, establishing an air pocket between the suction location and the filter surface during elution and minimizing the mixing interface between the aqueous and gaseous phases. Therefore, air passing through the filter has no opportunity to mix with liquid directly, limiting the formation of air bubbles and subsequent pathogen aerosolization. The addition of the extension tube in the filter housing further decreased the dead volume in the reusable housing (Fig. 6b). During elution, the liquid is extracted from the bottom of the filter housing to the outlet, reducing the amount of eluate left in the housing after the elution procedure from 20 to 7 mL.

Elution Device Design
In the initial work, a peristaltic pump was used for elution of the samples from the ViroCap filter. While functional, the pump did not enclose the system, which was a safety concern as there was the potential for aerosolization of biologicals from the sample and cross-contamination due to the system not being fully enclosed (Walls et al. 2014). Also, peristaltic pumps are expensive, not easily transferable internationally due to electrical socket and voltage requirements, and complicated to fix with internal and electronic components. Therefore, an elution apparatus was developed to replace the peristaltic pump and for deployment of the self-contained unit to international collaborators. This apparatus does not use electrical power, has a compact footprint (0.093 m 2 ), and is compatible with autoclave systems and biosafety cabinets available at the international collaborators' facilities. In combination with the modified filter housing, the elution device reduced the potential for cross-contamination and increased technician safety, while maintaining similar elution times. A low-cost ($20-$40) manual bilge pump created a vacuum, which exhibited low resistance and did not require significant force to perform the elution procedure. This Alfa Marine pump is not specific for the elution apparatus and so pumps from different manufacturers are compatible with the system, making replacement simple if needed. Use of the system was intuitive and required minimal training (typically less than 30 min). In addition, the elution device encloses the system outlet to reduce the possibility of cross-contamination in the laboratory. The elution device collected the sample into an enclosed sample collection cup; in combination with the optimized filter housing, the possibility of escape of pathogen-containing aerosols from the system was removed (Fig. 3d). If a small amount of bubbles or foam is present in the collection after the elution procedure, the user can wait less than 5 min for the foam to settle in the enclosed container before replacing the ported lid of the elution apparatus with the solid lid for storage and transport of the sample. All components that come in contact with the samples are autoclavable. Finally, the active personnel time required for a single elution using this pump (13 min, n = 6) was similar to that using the peristaltic pump (13 min, n = 6).

Virus Recovery Efficiency and Concentration Factor of the BMFS
In addition to modifying the BMFS design to improve usability and performance, the sensitivity of the BMFS was also determined. PV1 recovery measured 39.6 ± 9.6% (n = 18) for BMFS samples eluted from ViroCap filters in disposable housings using a single elution (eluate volume 175 mL). With the switch to the reusable housings, the PV1 recovery was 9.7 ± 2.0% (n = 21) using a single elution (eluate volume 100 mL). This recovery efficiency was significantly lower than the PV1 recovery with the disposable housing (p = 3.9 9 10 -6 , Student's t test). Due to the PV1 recovery decrease and a decrease in the eluate pH, the elution protocol was modified. The new double elution method included two elution steps, each with a hold time of 15 min rather than 30 min and an eluent volume of 100 mL. Using this double elution (total eluate volume 200 mL) and the reusable housings, the PV1 recovery measured 33.1 ± 8.8% (n = 11). The PV1 recovery efficiency from the reusable housing using a double elution was not statistically different than the recovery efficiency from the disposable housing using a single elution (p = 0.33, Student's t test). The concentration factor and effective volume assayed for BMFS samples vary by sample volume and type ( Table 3). The BMFS can concentrate 3-10 L of wastewater and wastewater-impacted surface waters to 10 mL, depending on the water source (Fagnant et al. 2014). The grab sampling method recommended for polioviruses by the WHO concentrates 500 mL to a final volume of 10 mL using the polyethylene glycol/dextran concentration method (World Health Organization 2015). Therefore, in its current form the BMFS results in an increase of 5-16.7 times the effective volume assayed compared to volumes assayed for 500-mL two-phase grab samples (Table 3). Hence, the increase in effective assay volume may When using a single elution, volume after primary concentration would be 100 mL. Regardless of volume after primary concentration, the volume after secondary concentration is 10 mL b Primary concentration factor after double elution. When using a single elution, primary concentration factor is 100 mL c Primary concentration factor after double elution. When using a single elution, primary concentration factor is 30 mL d Grab sampling method followed by two-phase concentration method recommended for poliovirus sampling by the World Health Organization (WHO) e v e = v a 9 c 1 , as no secondary concentration is performed ultimately reduce the likelihood of false-negative samples, compared to samples processed by the WHO algorithm. Based on the volumes filtered during the field evaluation, approximately five times the effective volume was assayed with the BMFS compared to the effective volume assayed with the two-phase grab sample method. An average of 2.9 ± 0.4 L was filtered per sample (n = 54) (Fig. 7) using the disposable housings and the 6-L thirdgeneration collection bag design at the four sites in Nairobi. Similar volumes were filtered volumes when using the reusable housing.
This compact and transportable field sampling system for enteric viruses requires no external power source, either in the field or in the laboratory. Transitioning from laboratory samples to field samples presented new challenges with water matrix effects, making field testing vital during kit development for troubleshooting these practical challenges to maximize concentrated volumes. Ultimately, use of the BMFS routinely achieved a concentration factor five times greater than that of the currently recommended 0.5-L grab samples processed by the WHO algorithm. With multiple product design iterations, the BMFS now allows for larger filtered volumes in the field, eases sample processing in the laboratory, and may ultimately reduce the likelihood of false-negative samples compared to samples processed by the WHO algorithm, making it a valuable sampling system for surveillance of poliovirus and other enteroviruses in wastewater and wastewater-impacted surface waters.