Abstract
In pressure-assisted thermal sterilization process (PATS), most of the microbial inactivation occurs under a combination of high temperature and pressure conditions for which meaningful experimental isothermal/isobaric (static) survival data are rarely if ever available. Therefore, a kinetic survival model for the targeted microbe, the magnitudes of its parameters, and the pressure’s direct contribution to the process lethality, i.e., besides the processing temperature elevation, must be determined mathematically from experimental survival ratios obtained after the food cooling at the end of dynamic treatments. At least in principle, this can be achieved with the endpoints method, explained and demonstrated with the aid of simulated realistic dynamic temperature and pressure profiles. The pressure’s net (unmediated) contribution to the process lethality can be expressed as equivalent time at the processing temperature, or as the added number of decades’ reduction to the treatment’s final survival ratio had it been reached in a purely thermal process having the same temperature profile. The pressure’s role is also manifested in the coefficients of a special pressure dependency term incorporated into the dynamic inactivation kinetics model. This term indicates whether the process lethality rises monotonically with temperature and pressure or there exists an optimal combination of the two. The expanded rate model can be used to simulate and examine the efficacy of existing or contemplated PATS processes by varying the temperature and pressure profiles, and/or by modifying the targeted microbe’s survival parameters.
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References
Ahn J, Balasubramaniam VM, Yousef AE (2007) Inactivation kinetics of selected aerobic and anaerobic bacterial by pressure-assisted thermal processing. Intnl J Food Microbiol 113:321–329
Al-Ghamdi S, Sonar CR, Patel J, Albahr Z, Salbani SS (2020) High pressure-assisted thermal sterilization of low-acid fruit and vegetable purees: microbial safety, nutrient, quality, and packaging evaluation. Food Control (114):107233
Alderte-Tapia JA, Torres JA (2020) Enhancing the inactivation of bacterial spores during pressure-assisted thermal processing. Food Eng Rev
Balasubramaniam VM, Barbosa- Cánovas GV, Lelieveld HLM (2016) High pressure processing of food. Springer, New York
Barbosa-Cánovas GV, Medina-Meza I, Candoğan K, Bermúdez-Aguirre D (2014) Advanced retorting, microwave assisted thermal sterilization (MATS), and pressure assisted thermal sterilization (PATS) to process meat products. Meat Sci 98:420–434
Bermudez-Aguirre D, Corradini MG, Candogan K, Barbosa-Canovas GV (2016) High pressure processing in combination with high temperature and other preservation factors. In: Balasubramaniam VM, Barbosa-Canovas GV, Lelieveld HLM (eds) High pressure processing of food. Springer, New York, pp 193–215
Bull MK, Olivier SA, van Diepenbeek RJ, Kormelink F, Chapman B (2009) Synergistic inactivation of spores of proteolitic Clostridium botulinum strain by high pressure and heat is strain and product dependent. Appl Env Microbiol 75:434–444
Buzrul S (2007) Evaluation of different dose-response models for high hydrostatic pressure inactivation of microorganisms Foods 6:79
Corradini MG, Normand MD, Peleg M (2008) Prediction of an organism’s inactivation patterns from three single survival ratios determined at the end of three non-isothermal heat treatments. Intnl J Food Microbiol 126:98–111
Daryaei H, Yousef BVM (2016) Microbiological aspect of high-pressure processing of foods. In: Balasubramaniam VM, Barbosa- Cánovas GV, Lelieveld HLM (eds) (2016) High pressure processing of food. Springer, pp 271–294
Heinz V, Knorr D (1996) High hydrostatic pressure inactivation kinetics of bacillus subtilis cells by three state-model considering distributed resistance mechanisms. Food Biothechnol 10:149–161
Koseki S, Yamamoto K (2007) A novel approach to predicting microbial inactivation kinetics during high pressure processing. Food Microbiol 116:275–282
Margosch D, Ehrman MA, Buckow R, Heinz V, Vogel RF (2006) High-pressure-mediated survival of Clostridium botulinum endospores at high temperature. Appl Env Microbiol 72:3471–3481
Mathys A, Reinke K, Knorr D (2009) High pressure thermal sterilization –development and application of temperature controlled spores inactivation studies. High Pressure Res 29:3–7
Nguyen LT, Balasubramaniam VM (2014) Estimation of accumulated lethality under pressure-assisted thermal processing Food Bioproc Technol 7:633–644
Peleg M (2003) Microbial survival curves: interpretation, mathematical modeling and utilization. Comments Theor Biol 8:357–387
Peleg M (2012) On quantifying nonthermal effects on the lethality of pressure-assisted heat preservation processes. J Food Sci 77:R47–R56
Peleg M (2020) Endpoints method for predicting microbial inactivation, nutrients degradation and quality loss at high and ultra high temperatures. In: Food Safety Engineering, Demirci A, Feng H, Krishnamurthy K (eds). Springer, New York, pp 421–446
Peleg M, Normand MD, Corradini MG, van Asselt AJ, de Jong P, ter Steeg PF (2008) Estimating the heat resistance parameters of bacterial spores from their survival ratios at the end of UHT and other heat treatments. Crit Rev Food Sci Nutr 48:634–648
Rajan S, Pandrangi S, Balasubramaniam VM, Yousef AE (2006) Inactivation of Bacilus stearothermophilus spores in egg patties by pressure-assisted thermal processing. LWT 39:844–851
Ramaswamy HS, Shao Y (2010) High pressure destruction of Clostridium sporogenes spores in salmon slurry at elevated temperatures. Intnl J Food Protect 13:1074–1091
Reinke K, Elinger N, Berger D, Mathys A, Setlow P, Knorr D (2012) Structural analysis of high pressure treated Bacilus subtilis spores. Innov Food Sci Eng Technol 17:43–53
Sermant-Moreno V, Barbosa-Cánovas GV, Torres JA, Welti-Cjhanes J (2014) High-pressure processing kinetics for microbial and enzyme inactivation. Food Eng Rev 6:56–88
Stewart CM, Dunn CP, Keener L (2016) Pressure-assisted thermal sterilization validation. In: Balasubramaniam VM, Barbosa-Canovas GV, Lelieveld HLM (eds) High pressure processing of food. Springer, New York
Tassou CC, Fabagou EZ, Samaras FJ, Galiastatou P, Mallidis CG (2007) Temperature-assisted high hydrostatic pressure inactivation of Staphylococcus aureus in ham model system: evaluation in selective and nonselective medium. J Appl Microbiol 104:1764–1773
Van Boekel MAJS (2002) On the use of the Weibull model to describe thermal inactivation of microbial vegetative cells. Intnl J Food Microbiol 74:139–159
Wang Y, Ismail M, Farid M (2017) Processing of baby food using pressure-assisted thermal sterilization (PATS) and comparison with thermal treatment. High Pressure Res 37:579–593
Wimalaratne SK, Farid MM (2008) Pressure assisted thermal sterilization. Food Bioproduct Proc 35:312–316
Zhang H, Mittal GS (2008) Effect of high-pressure processing (HPP) on bacterial spores: an overview. Rood Rev Intnl 24:330–351
Acknowledgements
The author expresses his deep gratitude to Mark D Normand for programing the Endpoints Method and its implementation in Mathematica®.
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Peleg, M. Assessing the Pressure’s Direct Contribution to the Efficacy of Pressure-Assisted Thermal Sterilization. Food Eng Rev 14, 201–211 (2022). https://doi.org/10.1007/s12393-021-09303-x
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DOI: https://doi.org/10.1007/s12393-021-09303-x