Development and Optimization of a Multilayer Rat Purkinje Neuron Culture

Elucidation of the mechanisms involved in neurodegenerative diseases of the cerebellum has been hampered by the lack of robust single cell models to study Purkinje neurons and replicate at the same time in vivo features. Cerebellar Purkinje neurons are difficult to grow in dispersed cell culture, and only limited work has been done using rat cells. We developed a refined protocol for growing rat Purkinje neurons from embryonic and postnatal tissue ex vivo that results in well-developed, mature, functional, and synaptically active neurons. The rat Purkinje neurons generated are responsive to paracrine factors and genetic manipulation, allowing great experimental flexibility at the single-cell level. This ex vivo model can be used to investigate disease mechanisms that disturb Purkinje neuron morphology, function, and communication in high- and low-throughput screening formats.


Introduction
Neuronal culture systems enable the study of basic principles of the nervous system and disease mechanisms.Ex vivo studies of the neuronal network and its alterations during disease are often done in organotypic slice cultures, but single-cell analyses, genetic modifications, and longterm studies are difficult or impossible with this ex vivo approach.Dissociated neuronal cultures allow manipulation and observation of neurons at the single-cell level; however, the quality and survival of the cultured cells depend on several factors: these include animal species, age of tissue used to derive single cells, the surface onto which single cells are seeded and cultured, and co-factors that drive neuronal growth and development [1][2][3][4][5].To date, the majority of successful Purkinje neuron culture models have used embryonic mouse cerebelli [3,5].It has proven more difficult to culture and transgenically manipulate cells from rats [1,2,4,6], but rats are physiologically, immunologically, genetically, and morphologically closer to humans than are mice [6,7].Furthermore, outbred or transgenic rat models [8][9][10] mimic human neurodegenerative disease mechanisms and progressions more closely than mouse models do [11,12].
Neurodegeneration generally occurs in the adult or aged human brain; therefore, a dissociated culture system derived from mature rather than embryonic tissue is desirable in investigation of the mechanisms behind pathological processes.Nonetheless, previous attempts to culture functional dissociated neurons from late postnatal tissue have largely been unsuccessful [1].Our goal was, therefore, to develop a culture protocol that provided well-developed, mature, Ida Margrethe Uggerud and Torbjørn Kråkenes contributed equally.
Christian Alexander Vedeler and Manja Schubert shared last authorship.
functional, and synaptically active rat Purkinje neurons independent of tissue age that resulted in maximal experimental flexibility and the potential for high-throughput screening.We discovered four essential factors for success: use of a three-dimensional growth structure, pH stability, co-factor supplementation and not using tissue after postnatal day 10.Using more mature or adult tissue at postnatal day 14, 28, 60, and 180 did not, yield surviving Purkinje neurons.

Neuronal Culture Preparation
All procedures were performed according to the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals Norway (FOTS 20,135,149/20157494/20170001).Wistar Hannover GLAST rat pups (n = 328), embryonic day 18 (E18) to postnatal day 10 (P10), were used for neuronal culture preparation.Briefly, following anaesthesia and decapitation, the brains were rapidly transferred into preparation solution of ice cold EBSS (Gibco, #24,010,043) solution containing 0.5% glucose (Sigma, #G8769) and 10 mM HEPES (Gibco, #15,630,056).Under a dissection microscope, the meninges and medulla oblongata were carefully removed, and the cerebellum was separated from the pons and the midbrain.Depending on the culture, either only the cerebellum or the cerebellum including pons was transferred to a 15-mL tube containing 20 U/mL papain (Worthington, #LK003178) dissolved in preparation solution and warmed up to 36 °C.The tube containing the cerebellar tissue was placed into the incubator for 15 min at 36 °C with occasionally swirling to digest the tissue.The papain solution was carefully removed with a fire-polished Pasteur pipette, and the digestion reaction was stopped by addition of stop media (advanced DMEM/ F12 solution (Gibco, #12,634,010) containing 0.5% glucose (Sigma, #G8769) and 10% foetal bovine serum (FBS, Gibco, #10,500,064)), pre-warmed to 36 °C.After 5 min of deactivation, the stop media was removed and 250 µL growth media containing 10% FBS per cerebellum was added.The tissue/media suspension was pipetted up and down with a fire-polished Pasteur pipette at least 100 times until cells were separated.

Purkinje Neuron Layer
Neuronal cells can survive only within a narrow pH range.The effects of the extracellular and intracellular pH on numerous enzymes and channels is an extensive subject which is beyond the scope of this study.However, it has been found that acidification can inhibit important classes of synaptic channels such as VGCC [13], whereas alkalinisation increases neuronal activity [14].Purkinje neurons and unipolar brush cells of the cerebellum are rich in acidsensitive ion channels [15] and rich in proteins that control calcium and thereby activity.We have found that a neutral pH, in the slightly acid range of 6.8 to 7.0, was essential for cell survival of newly plated Purkinje neurons, and therefore, the support cell layer culture was fed 24 h prior to addition of the Purkinje neuron layer to ensure a pH in this range.E18-and P0-derived Purkinje neuron cultures were prepared by seeding 500.000cells per mL from the vermis and the flocculus of the cerebellum onto support cell layers of different in vitro ages.The P10-derived Purkinje neuron culture was prepared by seeding 750.000 cells per mL from only the vermis of the cerebellum onto the support cell layers of different in vitro ages.Depending on the plate, a different volume of single-cell suspension was added to the support cell culture per well: 500 µL for 6-well plates, 250 µL for 12-well plates, and 100 µL for 24-well plates.The support cell layer growth media was supplemented with insulin (Invitrogen, #12,585,014; 1:250, stock 4 mg/mL), progesterone (Sigma, #P8783, 1:2000, stock 80 mM), insulin-like growth factor 1 (IGF1; Promokine, #E-60840, 1:40,000, stock 1 µg/µL), and protein kinase C inhibitor K252a (Alomone, # K-150; IC 50 25 nM).In cultures that were maintained for more than 28 days in vitro, the IGF1 and progesterone concentrations were reduced to 10 ng/mL and 20 µM, respectively.K252a was supplemented for 21 days before the washout process started, its optimal concentration was experimentally determined for each culture type.In the first 10 days of culturing, the K252a concentration was 5 nM for E18-derived Purkinje neuron layers, 10 nM for P0-derived Purkinje neuron layers, and 25 nM for P10-derived Purkinje neuron layers.After 10 days, the K252a concentration was increased to 25 nM for all Purkinje neuron layers until day 21 in vitro.After 21 days in vitro (DIV), the washout phase of K252a was implemented by replacing half of the media with media that did not contain K252a.Half of the culture medium was replaced every 3.5 days for 6-well plates and every 2 days for 12-and 24-well plates.The experiments that determined different culture parameters and their impact on the Purkinje neuron yield were randomly performed, three to six times per experimental setting with five independent repeats for each group and condition.

Lentiviral Gene Editing
The full-length L7 promoter region (1005 bp) [16,17] was custom cloned by SBI System Bioscience into construct pCDH-L7-MCS-copGFP (#CS970S-1), and viral particles with a yield of 2.24 × 10^9 infectious units per µL were produced.Two viral transduction approaches were tested.First, freshly prepared cells of E18 or P0 rat cerebellum (suspended in growth media containing no serum) were incubated for 10 min at 36 °C with 1.22 × 10^6 viral particles per mL cell suspension and were then seeded onto the support cell layer culture.To grow the transduced neurons, either 12-well plates containing coverslips or 35-mm µ-dishes for live-cell imaging (Ibidi, #80,136) were used.Media was replaced after 3 days, and transfection efficiency was evaluated by live-cell microscopy 24 h post transfection, and then daily until 21 DIV.At 21 DIV, the scan interval was changed from daily to every 3 or 4 days for cultures of E18 or P0 rat cerebellum, respectively, until 169 DIV.In the second experimental approach, the lentiviral transduction was performed on neurons in culture.Cells were fed on 15 DIV and 29 DIV, for cultures of E18 or P0 rat cerebellum, respectively, and 1 day later 2.5 × 10^6 viral particles per mL were applied to the culture media.
The neuronal morphology of GFP-expressing Purkinje neurons was analysed by capturing 10 independent 3 × 3 tile scans (Zyla camera configuration: 2048 × 2048; objectives: CFI Plan Apochromat Lambda dry objective 10 (NA 0.45, pixel size 603 nm) or dry objective 20 (NA 0.75, pixel size 301 nm)) on an Andor Dragonfly microscope system (Oxford Instruments).The viral transduction of the cultures was repeated three times.

Dendritic Tree Branch Analysis
The dendritic development (length and order) of the Purkinje neurons was evaluated by analysing 10 Purkinje neurons per coverslip in 10 independent experiments using the ImageJ plugin Simple_Neurit_Tracer (SNT; Neuroanatomy) [19].This free software plugin is available at http:// fiji.sc/ Sim-ple_ Neuri te_ Tracer.The plugin allows manually selection of points along tubular structures in confocal microscope z-stack images to trace the dendritic skeleton of neurons.
The program marks the path between two branch points by determining the highest fluorophore emission intensity.This semi-automated process ensures that the right paths are chosen and traced.Each dendritic tree was evaluated with branching grades (orders) according to the Fujishima protocol [20].
The branch orders for each analyzed Purkinje neuron were determined as follows: Microscope Leica files (.lif) were converted to TIFF files and opened with the Fiji SNT plugin.The reconstruction of Purkinje neuron dendritic branching started with tracing of the primary dendrites by selecting the soma as the starting point and selecting the point of intersection between primary and dendrite segments.Tracing non-primary dendrites was done by selecting a new point on the next dendrite intersection or the ending point of the dendrite.This was performed for the whole dendritic arbor of each Purkinje neuron.The data were exported to Excel where the average length of dendrites of the same order, as well as the number of segments of the same order, was evaluated for each analyzed Purkinje neuron.

3D Representation of Dendrites and Synapses
3D surface visualization of synapses was performed using Oxford Instruments analysis software IMARIS 9.3.1 (Bitplane) and the filament tracer tool [21].The "surface" tool was applied to make a solid filled surface representation of the Purkinje neurons through the CALB1 or PCP2 staining, with the background subtraction option enabled.A threshold was selected that demarcated the neuron structure accurately while excluding background.For the synapse analyses, a 600 × 800 µm selection box was placed around the dendrite in each image, and surfaces were created for synapses within the selection box.The same threshold settings were used across all images, and individual surface data from each dendrite were exported for the 3D representation of the synapses.

Statistical Analysis
All conditions were tested in triplicate or quadruplicate, if not otherwise indicated.Data analysis and calculations were performed using the software Excel 2016 and Graph Pad Prism 7.0.Data are presented as means ± SEM.Statistical significance was determined using the non-parametric two-tailed paired Mann-Whitney's U test.The level of significance is indicated with asterisks: *p < 0.05; **p < 0.01; ***p < 0.001.
Next, we plated a mixture of cerebellar cells and cells of the pons derived from E18, P0 or P10 rat tissue onto PDL-coated coverslips as an initial support cell layer.After culturing the support layer for 7 to 48 days, we plated another cell layer, a pure cerebellar cell layer, on top.We found that the tissue age of cells used to grow the support cell layer (E18 to P10) had no impact on the Purkinje neuron yield of the Purkinje neuron layer (data not shown).However, the in vitro age of the support layer influenced survival of neurons in a manner that depended on the tissue age used to grow the Purkinje neuron layer.The highest survival rate for E18-derived Purkinje neurons was observed when plated onto a support cell layer of 14 DIV (1153 ± 324); for P0-derived Purkinje neurons a support layer of 21 DIV (477 ± 136) was optimal; and for P10derived Purkinje neurons, the optimal support layer age was 28 DIV (84 ± 4) (Fig. 1A).These findings indicate that the older the starting tissue of the Purkinje neuron layer is, the more mature the support layer must be to achieve a high survival rate of Purkinje neurons.We were not able to grow Purkinje neurons from more mature and adult tissue, such as postnatal day 14, 28, 60, and 180.Furthermore, we found the high survival rate in long-term Purkinje neuron culture could only be maintained when non-physiological pH fluctuations were avoided.The higher metabolic demand of the multilayer culture required a short cycle of media replacement.The optimized protocol involved 50% media replacement every 3.5 days for 6-well plates or every second day for 12-and 24-well plates.

Dendritic Morphology of Purkinje Neurons: Importance of Paracrine Factors and Protein Kinase C Activity
Despite the improvement in Purkinje neuron survival resulting from multilayer culture, the dendritic morphology of the embryonic and early postnatal derived Purkinje neurons did not reflect that observed in vivo.E18-and P0-derived Purkinje neurons had fewer and shorter branches than those of P10 rats (Fig. 1B).Purkinje neuron survival and early dendritic tree development are highly dependent on paracrine factors such as progesterone, insulin, and insulin-like growth factor 1 (IGF1), which are secreted by other cells or self-produced by Purkinje neurons during the development of the cerebellum [23][24][25].Addition of 40 µM progesterone to the growth medium led to increased branching of the dendritic trees in E18-derived Purkinje neurons (Fig. 1C) but had no impact on the branch structure of P0-and P10derived Purkinje neurons (data not shown).The supplementation of only insulin or IGF1 did not improve the branch development but did stabilize the long-term growth of the other cerebellar cell types (data not shown).Therefore, insulin, IGF1 and progesterone had to be administered to all three Purkinje neuron cultures to archive a high survival of Purkinje neurons in long-term culture (data not shown).
We next tested the impact of inhibition of calciumdependent protein kinase C (PKC) in the multilayer culture.PKC is known to be important for long-term anatomical maturation of the Purkinje neuron dendritic tree [26].PKC inhibition using the chemical antagonist K252a considerably improved dendritic branching of Purkinje neurons in E18and P0-derived Purkinje neuron layers (Fig. 1B and 1D).This effect was not seen in the P10-derived culture, however (Fig. 1D).Interestingly, PKC inhibition greatly improved the survival of P10-derived Purkinje neurons (by a factor of 28) in a concentration-dependent manner, and a concentration dependence was also seen for P0-derived Purkinje neurons (by a factor of 6; Fig. 1E).Inhibiting PKC activity had no effect on the survival rate of E18-derived Purkinje neurons (Fig. 1E).
Thus, to achieve a well-developed rat Purkinje neuron culture with a functional network the K252a concentration had to be adjusted to the used tissue age during the first 10 days in culture: K252a was used at 5 nM for E18-derived cultures, at 10 nM for P0-derived cultures, and at 25 nM for P10-derived cultures.At 10 DIV, the K252a concentration was set to 25 nM for all groups.At 22 DIV, the washout phase of K252a was begun, and the concentration of K252a dropped to zero at 28 DIV due to media replacement.At 28 DIV, the morphology of the Purkinje neurons closely resembled the in vivo morphology, and therefore, the IGF1 and progesterone concentrations were reduced by factors of 2.5 and 2, respectively, during subsequent culture.
Furthermore, we found that the cultured Purkinje neurons formed functional synapses that showed post-synaptic expression of voltage gated calcium channels (VGCCs), metabotropic glutamate receptor 1 (mGluR1), and post-synaptic density protein 95 (PSD95) (Fig. 2B, upper panel).Presynaptic contacts to the surrounding network were detected through synapses that were positive for glutamate-decarboxylase (GAD65), α-synuclein (SNCA), bassoon (BSN), and synapsin I (SYN1) (Fig. 2B, lower panel).The presence of these biomarkers indicates the maturity of both the Purkinje neurons and the surrounding network.
Next, we tested whether the visualized synaptic network is functional.In vivo, Purkinje neurons fire spontaneous action potentials at frequencies of around 40-50 Hz with a complex trimodal pattern of tonic firing, bursting, and silent modes that depend on the anatomy and functional maturity [27,28].We performed multi-electrode array recordings of E18-derived Purkinje neurons that were cultured in a 24-well multi-electrode array.Cultures grown in our Purkinje neuron multilayer culture media first showed spontaneous spike activity at 11 DIV (0.15 ± 0.03 Hz).The spike rate increased to 2.56 ± 0.59 Hz at 21 DIV.After 28 DIV, the spike activity became erratic with long periods of silence, but a frequency of 2.79 ± 0.55 Hz was maintained until 59 DIV (Fig. 2C).We observed both uniform and highly non-uniform spike intervals and trains with silent periods between bursts and spike frequencies of up to 140 Hz within the bursts.We also evaluated Purkinje neurons activity after exchanged into previously described organotypic brain slice culture media [22] at 28 DIV.These neurons did not show the erratic spike activity after 28 DIV.The organotypic brain slice culture media, which was developed to address the needs of a more mature neuronal network, stabilized the spike frequency at 6.35 ± 1.85 Hz through 63 DIV (data not shown).

Cell Type-Specific Genetic Engineering
Cell type-specific genetic engineering is often an important approach in studding disease mechanisms.Therefore, we evaluate the potential of our Purkinje neuron model system for this approach by using lentiviral particles that transduce a viral vector for expression of green fluorescence protein (GFP) driven by the L7 promoter specifically into Purkinje neurons [16,17].The viral L7-GFP particles were added to dissociated Purkinje neurons on the day of seeding.Within 6 days, we observed Purkinje neurons expressing GFP (Fig. 2D).At 14 DIV, 61.5% of the cultured Purkinje neurons expressed GFP with minimal off-target expression (< 0.02%; data not shown).No alterations were seen in the dendritic structure due to thus endogenous protein expression.We observed a stable expression of GFP through 169 DIV (Fig. 3A).A high transfection rate was also achieved when the lentiviral particles were added to the culture at 14 DIV and 28 DIV; however, transfection efficiency and expression level fell progressively the later the genetic manipulation was implemented (data not shown).Live cell imaging of GFP-positive cultured Purkinje neurons showed very similar development to that seen in vivo which is characterized by three phases [29,30].In our E18 culture, at DIV 0 to 7, we observed the 1 st phase, the fusion phase that happens from embryonic day 17 to postnatal day 5; at DIV 7 to 9, we saw the 2 nd phase, the phase of stellate cells with disoriented dendrites that occurs at postnatal day 5 to 7; and from DIV 9 to 23, we observed the 3 rd phase, the phase of orientation and flattering of the dendritic tree that starts at

Discussion
To investigate the mechanisms underlying neurodegenerative disorders, good model systems are vital.Rat and mouse models are commonly used in biomedical research [6,[8][9][10][11][12], and several protocols for generating cerebellar cultures from mice, including some with healthy Purkinje neurons, have been published [3,5].Rats are more physiologically, genetically, and morphologically similar to humans than are mice, and therefore, rat models mimic many human neurodegenerative disease mechanisms better than mouse models do [6-9, 22, 31, 32].Furthermore, rats have a bigger size and higher stress tolerance than mice, and therefore, operative approaches are easier with low environmental impact [6].
Rat cerebellar cell cultures have been of limited utility, however [1,2,4].In previously reported systems, the longterm Purkinje neuron survival and dendritic development in culture were very poor when using postnatal rat tissue [1]; therefore, only embryonic tissue has been used [1,2,4].Furthermore, the neuronal activity of long-term cultured Purkinje neurons has not been demonstrated [2,4].Here we present an ex vivo culture protocol that not only leads to mature and synaptically active rat Purkinje neurons in a complex and robust long-term culture system but also allows for maximal experimental flexibility.The combined use of a support layer and time-dependent addition of hormones, paracrine factors, and biochemical activity regulators such as progesterone, insulin, IGF1, and K252a gave ideal conditions to grow a balanced cerebellar network from either embryonic or postnatal tissue (Fig. 3).
We showed that four factors are important for long-term survival as well as well-developed Purkinje neurons: (1) the support layer, (2) pH stability, (3) co-factor supplements, and (4) starting tissue age.The support layer affects the pH stability and also provides important cell-to-cell contacts that drive the release of paracrine factors.We found that fluctuation of the pH into the basic range (> 7.5), often seen in monolayer culture, reduced the long-term survival of Purkinje neurons.Fluctuation into the acid range (< 6.5) was better tolerated but indicated a high metabolic demand and therefore the need for a shorter feeding cycle.The fluctuations of the pH are prevented in our protocol by preparing media immediately before use and by feeding the support layer 24 h before plating the Purkinje neuron layer.
Survival and dendritic tree development of the Purkinje neurons are highly dependent on secreted factors and cell-cell interactions.Paracrine factors such as progesterone, insulin, and IGF1 are released by the cell network in an age-dependent manner to ensure Purkinje neuron development and maturity as well as the stability of the surrounding neuronal network [23][24][25].This age-dependent release was clearly seen in our model, as we observed that that the Fig. 3 Optimal conditions for culture of rat Purkinje neurons.A Supplementation with IGF1 and progesterone induced a stable environment that resulted in high survival rates of Purkinje neurons, whereas PKC inhibition (with K252a) shaped dendritic tree development for E18 and P0 cultures or impacted survival for P10-derived culture.The K252a starting concentration depends on the tissue used to start the culture.During the washout phase (DIV 22-28), no K252a is added to the media.At 28 DIV, concentrations of IGF1 and progesterone are reduced.The protocol allows growth of a stable Purkinje neuron culture for up to 6 months (DIV 169) in 6-to 24-well formats.B The time point for placement of the Purkinje neuron layer depends on the age of tissue harvest.For E18, the highest survival was found when placed on a support layer of 14 DIV; for P0, the support layer should be 21 DIV; and for P10, the support layer should be 28 DIV Purkinje neuron culture derived from postnatal tissue needed a more mature support cell layer than embryonic-derived Purkinje neurons.Addition of progesterone improved the dendritic tree branching of E18-derived Purkinje neuron but did not alter the branching in the two postnatal cultures.
Calcium homeostasis of the neuronal network is also critical during development [26,29,33,34].Neuronal dendrites are generated during development by a series of processes involving extension and retraction of dendritic branches and subsequent stabilization of existing dendrites through synaptic connections and neuronal calcium homeostasis [34].Calcium-dependent PKC subtypes, activated by synaptic inputs from parallel fibres (granule cells) through mGluR1/4, trigger functional changes as well as long-term anatomical maturation of the Purkinje neuron dendritic tree during cerebellar development [26,29].Minimizing the PKC activity by the application of K252a improved the dendritic tree development of Purkinje neurons derived from E18 and P0 tissue and considerably improved the survival of Purkinje neurons that were derived from P10 tissue.These results are indicative of biphasic action of PKC during Purkinje neuron development.Furthermore, analyses of the Purkinje neuron culture under modulated PKC activity revealed well-developed synapses that were positive for P/Q-VGCC and mGluR1, markers indicative of maturity.Electrophysiological recordings also indicated that the cultured Purkinje neurons matured throughout the cultivation period.The spontaneous activity of the Purkinje neurons was very similar to activities detected in vivo recordings: Purkinje neurons fire spontaneous action potentials with a complex trimodal pattern of tonic firing, bursting, and silent modes [27,28].However, the fire frequencies of spontaneous action potentials in our multilayer culture was only one-tenth of the frequency (40-50 Hz) that was recorded in vivo [27,28].As the neuronal network of our model system is not as well organized as the in vivo network and consists of only an approximately thickness of 50 µm, the lower frequency was not unexpected.
We also demonstrated that the Purkinje neurons in our model system could be genetically edited.The GFP-positive Purkinje neurons in our E18 culture system developed similarly to stages previously characterised in vivo [29,30,34].We observed the fusion phase, the phase of stellate cells with disoriented dendrites, and the orientation and flattering of the dendritic tree.At 15 DIV, the E18-derived Purkinje neurons had a very complex dendritic arbour structure similar to that observed in P12 rats.We were unable to culture functional Purkinje neurons from rat tissue older than P10 under the optimized conditions.All attempts with tissue of age P14, P28, P60, and P180 failed.The live-cell imaging of the GFP tagged E18 Purkinje neurons at DIV15, which is equivalent to P12, showed that the dendritic arbore consisted of increasing numbers of fine branches that have entered the 3rd phase of development [29,30,34].Therefore, neurons older then P10 cannot be separated without breaking the fine branches and killing the cell.

Conclusion
In summary, the rat Purkinje neurons grown in a multilayer long-term culture were (1) positive for all tested synaptic markers; (2) electrically active; and (3) responsive to genetic manipulation.This demonstrates that the culture protocol is robust and versatile and can thus be used in a wide variety of cerebellar studies.For example, this culture model should prove useful as a high-throughput screening tool to investigate disease mechanisms as well as for testing of potential drugs.
Abbreviations E18: Embryonic day 18; DIV: Days in vitro; FBS: Foetal bovine serum; GAD65: Glutamate-decarboxylase 65; GFP: Green fluorescence protein; GLYT2: Glycine transporter 2; IGF1: Insulinlike growth factor 1; mGluR1: Metabotropic glutamate receptor 1; P0: Postnatal day 0; P10: Postnatal day 10; PKC: Protein kinase C; PDL: Poly-d-lysine; PSD95: Post-synaptic density protein 95; VGCC : Voltage-gated calcium channels were made.The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.To view a copy of this licence, visit http:// creat iveco mmons.org/ licen ses/ by/4.0/.

Fig. 1
Fig. 1 Evaluation of age-and co-factor-dependence of rat Purkinje neuron culture at 21 days in vitro.A Relationship of Purkinje neuron yield to in vitro age of the support cell layer (DIV 7 to 48) for E18-, P0-, and P10-derived Purkinje neurons.B Representative skeletons of E18-, P0-, and P10-derived Purkinje neurons with and without PKC antagonist K252a and of E18-derived cultures grown on support layer or not.Scale bar: 20 µm.C Representative skeletons of E18-derived

Table 1
Primary antibodies.The signal to noise ratio for the antibodies were evaluated for the following conditions: 4% PFA at pH 7.2 diluted in 100 mM PBS; 1.5% PFA at pH 6 diluted in 100 mM natrium acetate buffer (NaAcB)); without heat-induced antigen retrieval (HIAGR); and with HIAGR either TRIS-based (pH 9) or citric acid-based (pH 6).The best conditions for each used antibody are described below EnCorBio, EnCor Biotechnology; SYSY, synaptic systems; PN, Purkinje neuron