Abstract
Hydroxycinnamate-CoA quinate hydroxycinnamoyl transferase (HQT) enzyme affect plant secondary metabolism and are crucial for growth and development. To date, limited research on the genome-wide analysis of HQT family genes and their regulatory roles in chlorogenic acid (CGA) accumulation in leafy vegetable sweet potato is available. Here, a total of 58 HQT family genes in the sweet potato genome (named IbHQT) were identified and analyzed. We studied the chromosomal distribution, phylogenetic relationship, motifs distribution, collinearity, and cis-acting element analysis of HQT family genes. This study used two sweet potato varieties, high CGA content Fushu 7-6-14-7 (HC), and low CGA content Fushu 7-6 (LC). Based on the phylogenetic analysis, clade A was unique among the identified four clades as it contained HQT genes from various species. The chromosomal location and collinearity analysis revealed that tandem gene duplication may promote the IbHQT gene expansion and expression. The expression patterns and profile analysis showed changes in gene expression levels at different developmental stages and under cold, drought, and salt stress conditions. The expression analysis verified by qRT-PCR revealed that IbHQT genes were highly expressed in the HC variety leaves than in the LC variety. Furthermore, cloning and gene function analysis unveiled that IbHQT family genes are involved in the biosynthesis and accumulation of CGA in sweet-potato. This study expands our understanding of the regulatory role of HQT genes in sweet-potato and lays a foundation for further functional characterization and genetic breeding by engineering targeted HQT candidate genes in various sweet-potato varieties and other species.
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This work was supported by the National Key R & D Program of China (Grant Nos. 2018YFD1000700, 2018YFD1000705, 2019YFD1001300, 2019YFD1001305).
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MBM, investigation, validation, writing-original draft, writing-review & editing; RP, formal analysis, validation, writing-review & editing; YP, validation; RGM, validation, writing-original draft, writing-review & editing; AS, writing-review & editing; XSY, Resources, funding acquisition; WZ, conceptualization, writing-review & editing, funding acquisition, and supervision. All authors have read and agreed to the published version of the manuscript.
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12298_2023_1299_MOESM5_ESM.tif
Supplementary Fig. S1 Conserved motifs analysis of ‘DVJKKALAKALVSYYPLAGRL’ sequence denoted by serial number ‘motif 4’ (TIF 1950 KB)
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Supplementary Fig. S2 Analysis of cis-acting elements in the promoter region of IbHQT genes. A large number of cis-acting elements are represented in different colors and the elements are shown on the right (TIF 5052 KB)
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Medison, M.B., Pan, R., Peng, Y. et al. Identification of HQT gene family and their potential function in CGA synthesis and abiotic stresses tolerance in vegetable sweet potato. Physiol Mol Biol Plants 29, 361–376 (2023). https://doi.org/10.1007/s12298-023-01299-4
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DOI: https://doi.org/10.1007/s12298-023-01299-4