Homeobox Gene Six3 is Required for the Differentiation of D2-Type Medium Spiny Neurons

Medium spiny neurons (MSNs) in the striatum, which can be divided into D1 and D2 MSNs, originate from the lateral ganglionic eminence (LGE). Previously, we reported that Six3 is a downstream target of Sp8/Sp9 in the transcriptional regulatory cascade of D2 MSN development and that conditionally knocking out Six3 leads to a severe loss of D2 MSNs. Here, we showed that Six3 mainly functions in D2 MSN precursor cells and gradually loses its function as D2 MSNs mature. Conditional deletion of Six3 had little effect on cell proliferation but blocked the differentiation of D2 MSN precursor cells. In addition, conditional overexpression of Six3 promoted the differentiation of precursor cells in the LGE. We measured an increase of apoptosis in the postnatal striatum of conditional Six3-knockout mice. This suggests that, in the absence of Six3, abnormally differentiated D2 MSNs are eliminated by programmed cell death. These results further identify Six3 as an important regulatory element during D2 MSN differentiation.

The ventral lateral ganglionic eminence (vLGE) is the origin of striatal MSNs, while the dorsal LGE (dLGE) mainly generates olfactory bulb interneurons [12,13]. Based on gene-expression patterns, it has been suggested that the vLGE can be divided into the pLGE3 and pLGE4 (progenitor LGE), and the dLGE can be divided into the pLGE1 and pLGE2 [14]. A variety of transcription factors regulate the development of the LGE. In the dLGE, Pax6 regulates the development of neurons expressing tyrosine hydroxylase in the olfactory bulb [15,16]. Sp8 and Sp9 are required for the production and survival, as well as the tangential and radial migration of interneurons in the olfactory bulb [12]. In the vLGE, early overexpression of the Gsx2 gene induces the generation of striatal MSNs. Gsx2-null mutants have significantly reduced generation of  [17,18]. Progenitor cell differentiation is blocked in the LGE subventricular zone (SVZ) of Dlx1/2 mutant mice [19,20]. D1 and D2 MSNs are also regulated by specific transcription factors. Isl1, Ebf1, and Zfhx3 regulate the development of D1 MSNs [9,[21][22][23][24].
Recently, we reported that Sp8 and Sp9 are coordinated to regulate D2 MSN generation, differentiation, and survival. Six3 expression in the LGE is significantly decreased in Sp8-and Sp9-knockout mice. Accordingly, conditional knockout of Six3 results in a significant reduction in the number of D2 MSNs, similar to the phenotype in the striatum of Sp8-and Sp9-knockout mice [25,26]. The homeobox transcription factor Six3, which contains a conserved Six domain and a Six-type homeobox domain, belongs to the Six gene family [27]. Six3 is expressed as early as E6.5-E7.0 at the most anterior region of the embryo and plays important roles in the development of the forebrain and visual system [28][29][30][31]. Mutation of Six3 causes holoprosencephaly [32,33]. Six3 is also expressed in ependymal cells, and its dysfunction leads to the inability of ependymal cells to inhibit radial glial activity, which leads to developmental defects of the lateral ventricle wall and abnormal neuroblast migration and differentiation [34]. As a direct downstream target of SP9 and SP8, the transcription factor Six3 is required for the production of D2 MSNs [26], but the cellular and molecular mechanisms were unknown.
In this study, we investigated the mechanism underlying the effect of a reduction in the number of D2 MSNs in Dlx5/6-CIE, Six3 flox/flox (referred to as Six3-cKO) mice. We found that knocked out Six3 in progenitor cells using Nestin-Cre line resulted in severe D2 MSN developmental defect, consistent with the results of Dlx5/6-CIE line, but not in Drd2-Cre line, which eliminate Six3 in immature D2 MSNs, indicating Six3 primarily function in progenitor cells at embryonic stage. The reduction in the number of D2 MSNs in Six3-cKO mice mainly ascribe to the abnormal differentiation, but not proliferation defect, of progenitor cells, identifying Six3 as an important regulatory element of MSN development. These findings broaden our comprehension of the transcriptional mechanisms underlying the development of striatal projection neurons.

Animals
All experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by Animal Ethics Committee of Fudan University. We generated mice that conditionally overexpressed Six3 by knocking CAG-promoter-Flox-STOP-Flox-Six3-IRES-Lacz into the Rosa26 locus. Dlx5/6-CIE [35], Nestin-Cre [36,37], Six3 floxed [26,38], and Drd2-Cre mice (from the Mutant Mouse Resource and Research Center) [39] were previously described. Wild-type, Dlx5/6-CIE, Drd2-Cre; Six3 F/? and Six3 floxed littermate mice without the Cre allele were used as controls. These mice were on mixed genetic backgrounds of C57BL/6J, 129S6, and CD1. The day on which a vaginal plug was detected was considered embryonic day 0.5 (E0.5), and the day of birth was calculated as postnatal day 0 (P0).

Immunohistochemistry
Immunohistochemistry was performed as previously described [26]. Briefly, postnatal and embryonic brains were collected and placed in 4% paraformaldehyde overnight at 4°C, cryoprotected in 30% sucrose for at least 24 h, frozen in optimal cutting temperature and cryosectioned. All tissues were sectioned coronally at 12 or 20 lm and stained on glass slides.

In situ RNA Hybridization
In situ hybridization was performed on 20-lm cryostat sections as previously described using digoxigenin-labeled riboprobes [25,26]. Riboprobes were amplified by PCR using the following primers: Drd2 forward: CGGGAGCTGGAAGCCTCGA

Microscopy
Images were captured using an Olympus BX 51 microscope or an Olympus FV1000 confocal microscope system. FV10-ASW software was used to reconstruct the Z-stack confocal images. All images were merged, cropped, and optimized equally using Adobe Photoshop CC.

Quantification and Statistics
The numbers of Drd2-, Adora2a-, Drd1-, and Tac1positive cells in the striatum were counted in 3 randomly-chosen 20-lm sections from each mouse. Three or four control and Six3 conditional knockout mice from each group were analyzed at P11.
The number of FOXP1 ? cells and the integrated density of Ebf1 and Isl1 measured by ImageJ in the LGE SVZ were quantified in 3 randomly-chosen 12-lm or 20-lm sections from each mouse. Three or four Dlx5/6-CIE control and Six3-cKO mice from each group were analyzed at E16.5.
The numbers of BCL11B ? and BCL11B ? /EBF1 ? cells in the striatum was counted in 3 randomly-chosen 12-lm sections from each mouse. Three Dlx5/6-CIE control and Six3-cKO mice from each group were analyzed at P0.
The number of FOXP1 ? cells and the integrated density of BCL11B measured by ImageJ in the LGE SVZ were quantified in 3 randomly-chosen 12-lm sections from each mouse, and three Dlx5/6-CIE control and Dlx5/6-CIE, Rosa-Six3OE/? mice from each group were analyzed at E14.5 and E16.5.
The numbers of BrdU ? and KI67 ? cells in the LGE were counted in 3 randomly-chosen 20-lm sections from each mouse, and 3 control and Six3-cKO mice from each group were analyzed at E14.5 or E16.5.
The numbers of SP8 ? and SP9 ? cells in the LGE SVZ were counted in 3 randomly-chosen 12-lm sections from each mouse. Three or four Dlx5/6-CIE control and Six3-cKO mice from each group were analyzed at E14.5 or E16.5. The integrated density of Six3OS, Adora2a, and Drd2 measured by ImageJ in the LGE or striatum were quantified in 3 randomly chosen 20 lm sections from each mouse. Three or four Dlx5/6-CIE control and Six3-cKO mice from each group were analyzed at E16.5 or P0.
The number of cleaved Caspase-3 ? cells in the striatum was counted in 3 randomly-chosen 20-lm sections from each mouse, and 3 Dlx5/6-CIE control and Six3-cKO mice from each group were analyzed at P0, P3, P7, and P11.

Six3 Mainly Functions in D2 MSN Precursor Cells
We previously reported that the number of striatal D2 MSNs is significantly decreased in Six3 conditional knockout mice [26]. We crossed Six3 floxed mice with Nestin-Cre and Drd2-Cre lines to further examine the function of Six3 (Fig. 1). According to our previously published paper, SIX3 is prominently expressed in the pLGE3 domain of the SVZ and scattered in the LGE mantle zone (MZ) at the embryonic stage. Its expression is later than that of Sp9 but earlier than that of Drd2-EGFP. The expression of Six3 is rapidly down-regulated in the striatum at the postnatal stage [26]. The Nestin-Cre line, in which Six3 was knocked out earlier than in Dlx5/6-CIE mice, expressed CRE protein in neural stem cells in the LGE [40][41][42], whereas in the Drd2-Cre line, Six3 was deleted in immature D2 MSNs after it was expressed for a short time, as Drd2 was expressed later than Six3 in the LGE [26]. At P11, the volume of the lateral ventricles was increased while that of the striatum was reduced in Nestin-Cre, Six3 F/F mice compared with wild-type control mice (Fig. 1A). This is consistent with our previous results [26]. The numbers of Drd2 ? and Adora2a ? MSNs in the striatum of Nestin-Cre, Six3 F/F mice were greatly decreased compared with those in control mice. The reductions of Drd2 ? and Adora2a ? MSNs mainly occurred in the medial striatum, indicating that the generation of late-born D2 MSNs was compromised in Nestin-Cre, Six3 F/F mice (Fig. 1A). The numbers of Drd1 ? and Tac1 ? cells, though significantly decreased, were relatively less affected (Fig. 1A). These decreased Drd1 ? and Tac1 ? cells might be ascribed to an ependymal cell defect since the development of ependymal cells was disturbed in Nestin-Cre, Six3 F/F mice [34]. In contrast, Drd2 ? , Adora2a ? and Drd1 ? , and Tac1 ? MSNs were densely distributed in the striatum of both Drd2-Cre, Six3 F/F mice and Drd2-Cre, Six3 F/? control mice at P11 (Fig. 1B). The numbers of both D1 and D2 MSNs were comparable in Drd2-Cre, Six3 F/F mice and Drd2-Cre, Six3 F/? control mice at P11 (Fig. 1B).
This indicates that the development of D2 MSNs is unaffected when Six3 function is blocked in immature D2 MSNs. These results suggest that Six3 mainly functions before D2 MSNs differentiate and gradually loses its function as they mature.

D2 MSN Neurogenesis is Reduced in the LGE SVZ of Six3-cKO Mice
To investigate the cause of the significant reduction in the number of striatal D2 MSNs in the absence of Six3, we further examined neurogenesis in the striatum of Six3-cKO and Dlx5/6-CIE control mice. The LGE SVZ at later developmental stages contains proliferating cells and differentiated cells [43]. FOXP1 ? cells in the LGE SVZ are differentiated newborn MSNs [44]. We found that the expression of FOXP1 in the LGE SVZ was severely reduced in Six3-cKO mice compared to control mice at E16.5 ( Fig. 2A), indicating a reduction of neurogenesis in both D1 and D2 MSNs. Consistent with this result, we found that the D1 MSN-specific marker Ebf1 [9] was significantly reduced in the LGE SVZ of Six3-cKO mice at E16.5 ( Fig. 2A). The expression of Isl1, another D1 MSNspecific marker [9], was also greatly reduced in the LGE SVZ of Six3-cKO mice compared to that in control mice at E16.5 ( Fig. 2A).
We next examined the number of striatal MSNs at P0 using BCL11B, a pan-striatal MSN marker, combined with EBF1 expression to distinguish D1 MSNs from D2 MSNs [9,45]. BCL11B ? /EBF1 ? cells represented D1 MSNs, BCL11B ? and EBF1 immuno-negative (BCL11B ? / EBF1 -) cells represented D2 MSNs (Fig. 2B). We found no significant difference in the number of EBF1 ? cells (D1 MSNs), but the number of BCL11B ? /EBF1cells (D2 MSNs) was significantly lower in Six3-cKO mice than in controls at P0 (Fig. 2B). These results suggest that neurogenesis of D1 MSNs is reduced at the embryonic stage, but not at the postnatal stage. This indicates that the absence of Six3 results in a subpopulation of D1 MSNs with delayed differentiation at the embryonic stage. In contrast, the neurogenesis of D2 MSNs was significantly decreased in the LGE of Six3-cKO mice from the embryonic to the postnatal stage (Figs 1A and 2).

Cell Proliferation is Unaffected in the LGE of Six3-cKO Mice
Next, we determined whether LGE cell proliferation was changed in Six3-cKO mice, as a small population of SIX3 ? cells were in S-phase, and Six3 has been reported to regulate cell proliferation [46,47]. It is possible that Six3 functions by regulating the cell cycle during LGE development. We performed 30-min BrdU pulse-labeling experiments at E14.5 and E16.5 (Fig. 3A, B). The number of BrdU ? cells was comparable in Six3-cKO and control mice at E14.5 (Fig. 3A). Consistent with this, the data showed that the total number of BrdU ? cells in the LGE was also unchanged in the LGE of Six3-cKO mice compared to that of control mice at E16.5 (Fig. 3B). Notably, BrdU ? cells seemed to accumulate in the ventricular zone (VZ) at E14.5 and E16.5, since we saw more BrdU ? cells (but no significant difference) in the VZ of Six3-cKO mice (Fig. 3B). Because Dlx5/6-CIE mice expressed little CRE in the VZ, we proposed that the slight accumulation of BrdU ? cells in the VZ was a secondary effect of the blocked differentiation of progenitor cells in the LGE SVZ. We also found that the expression of KI67, a classical cell proliferation marker, in the LGE was slightly but not significantly higher in Six3-cKO mice than that in control mice (Fig. 3C). These results suggest that cell proliferation during LGE development is little affected in the absence of Six3 function.

Differentiation of D2 MSN Precursor Cells is Blocked in Six3-cKO Mice
Since cell proliferation was not compromised in Six3-cKO mice, we hypothesized that the significant loss of D2 MSNs was due to abnormal differentiation of precursor cells. We assessed Sp8 and Sp9 expression in the LGE as they are expressed earlier than Six3 and are upstream of it in the LGE SVZ [26]. Normally, cells with high SP8 expression were located in the dLGE SVZ, while cells with low expression level of SP8 were located in the vLGE SVZ at E14.5 and E16.5 (Fig. 4A). SP9 ? cells located in the SVZ were mainly precursor cells, and those in the MZ were mainly D2 MSNs [25]. SP8 ? cells were significantly higher in the vLGE SVZ in Six3-cKO embryos than in controls at E14.5 and E16.5 (Fig. 4A). The number of SP9 ? cells was also significantly increased in the LGE SVZ of Six3-cKO embryos at E16.5 (Fig. 4A). The increased number of SP8 ? and SP9 ? cells in the LGE SVZ indicated that MSN precursor cells accumulated in Six3-cKO embryos. Although SP8 ? and SP9 ? precursor cells accumulated in the LGE SVZ, we still observed many SP9 ? cells in the LGE MZ of Six3-cKO embryos (Fig. 4A). Because SP9 is mainly expressed in D2 MSNs in the striatum [25], these SP9 ? cells in the LGE MZ of Six3-cKO embryos were putative D2 MSNs.
As described above, BCL11B ? /EBF1cells in the striatum were visualized as white dots (Fig. 2B). The numbers of BCL11B ? /EBF1cells was lower in Six3-cKO mice than in controls (Fig 2B), but there were still many BCL11B ? /EBF1cells in the striatum (Fig. 2B). This also indicated that putative D2 MSNs were generated in the LGE MZ of Six3-cKO mice, consistent with above result (Fig 4A). Besides, the ratio of D1 MSNs was increased while that of D2 MSNs was decreased in Six3-cKO mice, indicating that losing Six3 function in LGE leads to abnormal neural differentiation.
Six3OS has been reported to be co-expressed with Six3 in the hypothalamus and retina to indirectly regulate the function of Six3 [48,49]. We immunostained for SIX3 after in situ hybridization for Six3OS mRNA, and found that most, if not all, Six3OS ? cells expressed SIX3 (Fig. 4B). This indicated that Six3OS and SIX3 are expressed in the same cell type in the LGE SVZ. Six3OS was strongly expressed in the LGE SVZ but seldom in the MZ of control mice (Fig. 4C), suggesting that Six3OS is mainly expressed in precursor cells. Six3OS expression was significantly up-regulated in the LGE SVZ of Six3-cKO mice (Fig. 4C), indicating that Six3OS ? precursor cells accumulate in the LGE SVZ in the absence of Six3. Surprisingly, we also found that many Six3OS ? cells were located in the LGE MZ or striatum at E16.5 and P0, suggesting that Six3OS expression in those precursor cells is not down-regulated and they then migrated into the MZ or striatum with insufficient maturation after loss of Six3 (Fig. 4C). Once again, Six3OS ? cells in the MZ indicated that Six3OS ? putative D2 MSNs are generated in the LGE of Six3-cKO mice, consistent with the above results.
We then examined the expression of the differentiated D2 MSN markers Drd2 and Adora2a at E16.5 and P0 (Fig. 4D). Drd2 and Adora2a were strongly expressed in the LGE SVZ and MZ in control mice (Fig. 4D). However, only a few cells expressing high levels of Drd2 and very few Adora2a ? cells were located in the LGE MZ of Six3-cKO mice at E16.5 (Fig. 4D). Similarly, Drd2 and Adora2a were strongly expressed in the striatum of control mice but significantly reduced in that of Six3-cKO mice at P0 (Fig. 4D). We previously reported that the cells that express Drd2 strongly are mainly striatal cholinergic interneurons [25,26]. Thus, SP8 ? and SP9 ? precursor cells accumulated in the LGE SVZ, SP9 ? cells and many BCL11B ? /EBF1putative D2 MSNs located in the LGE MZ and striatum, but few Drd2 ? and Adora2a ? cells were found in the LGE and striatum, providing strong evidence that D2 MSN precursor cells differentiate abnormally in the striatum without Six3.
We used BCL11B and FOXP1 to assess whether Six3 overexpression promotes precursor cell differentiation (Fig. 6). Both BCL11B and FOXP1 were weakly expressed in the LGE SVZ but strongly expressed in the MZ of Dlx5/ 6-CIE control mice (Fig. 6). We found that the expression of BCL11B was significantly increased in the LGE SVZ of Six3 conditional overexpression mice at E14.5 (Fig. 6A). Similarly, very few FOXP1 ? cells were located in the LGE SVZ of control mice, but many FOXP1 ? cells were distributed in the LGE SVZ of Six3 conditional overexpression mice at E14.5 (Fig. 6A). The quantification confirmed that the number of FOXP1 ? cells was significantly higher in the LGE SVZ of Six3 conditional overexpression mice than in controls (Fig. 6A). We also analyzed BCL11B and FOXP1 expression at E16.5 (Fig. 6B). The expression of BCL11B in the LGE SVZ of Dlx5/6-CIE, Rosa-Six3OE/? mice was slightly upregulated compared to controls (Fig. 6B). We confirmed this phenotype by counting the FOXP1 ? cells in the LGE SVZ and found that the number of these cells was significantly increased in Dlx5/6-CIE, Rosa-Six3OE/? mice (Fig. 6B). These results suggested that Six3 promotes precursor cell differentiation in the LGE.

Loss of Six3 Induces Apoptosis in the Postnatal Striatum
We analyzed cell death by evaluating cleaved Caspase-3 expression to investigate whether abnormally differentiated D2 MSNs survived in the striatum of postnatal Six3-cKO mice. The data showed that in control mice, the number of Caspase-3 ? cells increased from P0, peaked at P3, and then decreased to a very low level as the striatum developed (Fig. 7). Without Six3 function, we found that the number of Caspase-3 ? cells was significantly higher than in  controls at P0 and P3, but that there was no significant difference at P7 or P11 (Fig. 7). We inferred that most of the dying cells were abnormally-differentiated D2 MSN precursor cells and that these cells were finally eliminated by programmed cell death, as we did not find a severe reduction in the number of D1 MSNs in Six3-cKO mice (Fig. 1).

Discussion
Striatal MSNs originate from the vLGE, while olfactory bulb interneurons are generated in the dLGE [13]. The LGE VZ was subdivided into 4 domains according to the combinatorial expression of several transcription factors. Pax6, Gsx2, and Er81 (Etv1) were used to identify the dLGE. The vLGE contained the pLGE3, in which Isl1 was strongly expressed while there was no expression of Er81, and the pLGE4, in which Nkx6.2 was strongly expressed while there was little expression of Isl1. The development of the two types of MSNs is regulated by many transcription factors [14]. For example, Gsx2, Ascl1, and Dlx1/2 are required for pan-striatal MSN development [50]; EBf1 and Isl1 specifically regulate D1 MSN differentiation and axonal guidance [9,[21][22][23]; Sp8 and Sp9 regulate D2 MSN generation and survival [25,26]. Here, we found that Six3 is required for D2 MSN differentiation and that its function is gradually reduced during D2 MSN maturation.
Six3 is expressed in ependymal cells of postnatal mice, and is essential for the maturation of ependymal cells. In mice in which Six3 is conditionally knocked out by Nestin-Cre, cells located in the lateral ventricle wall contain mixed characteristics of ependymal cells and radial glia [34]. These defective cells result in the abnormal migration and differentiation of neuroblasts, markedly enlarged lateral ventricles, and hydrocephalus [34]. In this study, we obtained the same results, i.e., a reduction in the number of D2 MSNs and enlarged lateral ventricles, in both Dlx5/6-CIE, Six3 F/F and Nestin-Cre, Six3 F/F mice. The Six3 gene was deleted in ependymal cells in Nestin-Cre, Six3 F/F mice, but not in Dlx5/6-CIE, Six3 F/F mice, as Dlx5/6 were rarely expressed in the progenitors (neural stem cells) of ependymal cells. However, the lateral ventricles of both Dlx5/6-CIE, Six3 F/F and Nestin-Cre, Six3 F/F mice were significantly enlarged, consistent with our previous report on Sp9 mutant mice [25,26]. This might indicate that enlargement of the lateral ventricle in Nestin-Cre, Six3 F/F mice is caused by the significant reduction in the number of D2 MSNs in the striatum.
It has been reported that Isl1, the conditional knockout of which results in a significant reduction in the number of D1 MSNs, regulates the development of D1 MSNs through semaphorin 3E (Sema3e) signaling, and that Ebf1 also regulates the differentiation of D1 MSNs [9,21,22]. We previously showed that few ISL1 ? or EBF1 ? cells express the SIX3 protein [26]. In this study, we found that the numbers of Isl1 ? and Ebf1 ? cells were reduced in the LGE SVZ of Six3-cKO mice at E16.5 but that there was no significant difference at P0. The accumulation of progenitor cells such as Ascl1 ? cells in the LGE SVZ of Six3-cKO mice may retard differentiation by enhancing the Notching signal. We propose that Six3 might cell-nonautonomously promote the differentiation of a subpopulation of D1 MSNs that experience delayed differentiation to a certain degree when Six3 is knocked out in the LGE SVZ.
Six3 was mainly expressed in precursor cells and newborn immature D2 MSNs in the LGE SVZ. Loss of Six3 function in progenitor cells resulted in a significant reduction in the number of mature D2 MSNs, whereas Six3-knockout in differentiated D2 MSNs (Drd2 ? ) had little effect on striatal MSN development. This indicates that the functions of Six3 decrease as D2 MSNs mature. The increased apoptosis in the absence of Six3 may be because Six3 is required for the survival of immature D2 MSNs, since SIX3 is expressed in immature D2 MSNs. However, in Drd2-cre, Six3 F/F mice, in which Six3 was deleted in immature D2 MSNs, the number of D2 MSNs was comparable to that of control mice. This demonstrates that Six3 plays a minor role in immature D2 MSN survival. Apart from that, large numbers of SP9 ? , Six3OS ? putative D2 MSNs were found in the LGE SVZ and MZ of Six3-cKO mice. But few Drd2 ? , Adora2a ? immature D2 MSNs were observed. This suggests that the differentiation of precursor cells is blocked in the absence of Six3. Thus, the increased apoptosis and reduction in the number of D2 MSNs in the absence of Six3 occurs mainly in response to the abnormal differentiation of D2 MSNs.
Here, we found that Six3OS was also co-expressed with Six3 in the LGE SVZ but down-regulated in the LGE MZ.
Six3 was strongly expressed in the LGE SVZ and its expression was scattered in the LGE MZ. The difference in expression patterns between Six3 and Six3OS indicates that they have different functions in LGE development. The Six3OS/Six3 co-expression pattern in the LGE SVZ indicates that Six3OS ? /Six3 ? cells are precursor cells and that Six3OS -/Six3 ? cells are differentiated D2 MSNs. Thus, the accumulation of many Six3OS ? progenitor cells in the LGE SVZ of Six3-cKO mice suggests that D2 MSN differentiation was blocked due to the loss of Six3; however, the migration of D2 MSNs was less affected in Six3-cKO mice, as we found that many Six3OS ? and BCL11B ? /EBFcells were distributed in the LGE MZ.
It is noteworthy that the numbers of Drd2 ? and Adora2a ? cells in the LGE MZ at E16.5 and P0 were significantly fewer than those in the Six3-cKO striatum at P11, indicating that a small population of precursor cells differentiates into mature D2 MSNs postnatally. We hypothesize that these mature D2 MSNs are largely generated in the pLGE4, as Six3 is most prominently expressed in the pLGE3. Thus, we propose that most D2 MSNs are generated in the pLGE3 and that a small number of D2 MSNs are derived from the pLGE4. Whether D2 MSNs with different origins exhibit different axonal guidance and functions requires further study. Our RNAseq data showed that expression of Six2, a homologous gene of Six3, in the LGE was significantly up-regulated in Six3-cKO mice compared to control mice (data not shown). This suggests that Six2 may have functional redundancy with Six3 and in turn partly promotes the generation of a subset of D2 MSNs.
In summary, in this study, we provide evidence that Six3 is an important regulatory element in the LGE SVZ, where it specifically promotes the differentiation of D2 MSN precursor cells. Ongoing studies are aimed at elucidating the molecular mechanisms underlying the distinct functions of Six3. These findings broaden our comprehension of the transcriptional mechanisms underlying the development of striatal projection neurons. source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons. org/licenses/by/4.0/.