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Discovery of a RuBisCO-like Protein that Functions as an Oxygenase in the Novel d-Hamamelose Pathway

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An Erratum to this article was published on 01 November 2018

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Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is the key catalyst of CO2 fixation in nature. RuBisCO forms I, II, and III catalyze CO2 fixation reactions, whereas form IV, also called the RuBisCO-like protein (RLP), is known to have no carboxylase or oxygenase activities. Here, we describe an RLP in Ochrobactrum anthropi ATCC 49188 (Oant_3067; HamA) that functions as an oxygenase in the metabolism of d-hamamelose, a branched-chain hexose found in most higher plants. The d-hamamelose pathway is comprised of five previously unknown enzymes: d-hamamelose dehydrogenase, d-hamamelono-lactonase, d-hamamelonate kinase, d-hamamelonate-2′,5-bisphosphate dehydrogenase (decarboxylating), and the RLP 3-keto-d-ribitol-1,5-bisphosphate (KRBP) oxygenase, which converts KRBP to 3-d-phosphoglycerate and phosphoglycolate. HamA represents the first RLP catalyzing the O2-dependent oxidative C–C bond cleavage reaction, and our findings may provide insights into its applications in oxidative cleavage of organic molecules.

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Change history

  • 17 January 2019

    In the 2018 issue of Biotechnology and Bioprocess Engineering (BBE), an error occurred in the research article: Suk Min Kim, Hyun Seung Lim, and Sun Bok Lee (2018) Discovery of a RuBisCO-like Protein that Functions as an Oxygenase in the Novel <Emphasis Type="SmallCaps">d</Emphasis>-Hamamelose Pathway. <Emphasis Type="Italic">Biotechnol. Bioprocess Eng.</Emphasis> 23: 490–499. In Fig. 3, <Emphasis Type="SmallCaps">l</Emphasis>-Lyxose and <Emphasis Type="SmallCaps">l</Emphasis>-Lyxonate should be replaced by <Emphasis Type="SmallCaps">d</Emphasis>-Ribose and <Emphasis Type="SmallCaps">d</Emphasis>-Ribonate, respectively.

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  1. Both authors contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea

    Suk Min Kim, Hyun Seung Lim & Sun Bok Lee

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  1. Suk Min Kim
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Correspondence to Sun Bok Lee.

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Kim, S.M., Lim, H.S. & Lee, S.B. Discovery of a RuBisCO-like Protein that Functions as an Oxygenase in the Novel d-Hamamelose Pathway. Biotechnol Bioproc E 23, 490–499 (2018). https://doi.org/10.1007/s12257-018-0305-6

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