Abstract
The recombinant Escherichia coli (E. coli) expressing human hepatic metallothionein_IA (hMT_IA) was constructed for bioaccumulation of Arsenic (As). The gene sequence of hMT_IA was modified for codon preference of E. coli and synthesized using chemical method. The vector of pGEX_4T_1 was used and hMT_IA was expressed as the fusion protein with glutathione S-transferase (GST) tag. The bioaccumulation capability of arsenite compounds As(III) of the recombinant E. coli increased more than 3-fold from 76.3 to 319.6 µg/g dry cells compared with the control. The conditions of 50 µM of As(III) and low pHs were optimal for As(III) bioaccumulation. The heavy metals of Cd, Hg, and Zn inhibited As(III) bioaccumulation. The bioaccumulation reached 70% of the saturated value within 1 h. The recombinant E. coli will be useful in bioremediation of arsenic or other kinds of heavy metal contaminated water.
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Macy, J. M., K. Nunan, K. D. Hagen, D. R. Dixon, P. J. Harbour, M. Cahill, and L. I. Sly (1996) Chrysiogenes arsenatis gen. nov., sp. nov., a new arsenate-respiring bacterium isolated from gold mine wastewater. Int. J. Syst. Bacteriol. 46: 1153–1157.
Kostal, J., R. Yang, C. H. Wu, A. Mulchandani, and W. Chen (2004) Enhanced arsenic accumulation in engineered bacterial cells expressing ArsR. Appl. Environ. Microbiol. 70: 4582–4587.
Han, F. X., Y. Su, D. L. Monts, M. J. Plodinec, A. Banin, and G. E. Triplett (2003) Assessment of global industrialage anthropogenic arsenic contamination. Naturwissenschaften. 90: 395–401.
Merrifield, M. E., T. Ngu, and M. J. Stillman (2004) Arsenic binding to Fucus vesiculosus metallothionein. Biochem. Biophys. Res. Commun. 324: 127–132.
Kessel, M., S. X. Liu, A. Xu, R. Santella, and T. K. Hei (2002) Arsenic induces oxidative DNA damage in mammalian cells. Mol. Cell. Biochem. 234-235: 301–308.
Ninomiya, M., T. Kajiguchi, K. Yamamoto, T. Kinoshita, N. Emi, and T. Naoe (2006) Increased oxidative DNA products in patients with acute promyelocytic leukemia during arsenic therapy. Haematologica. 91: 1571–1572.
Karagas, M. R., T. D. Tosteson, J. Blum, J. S. Morris, J. A. Baron, and B. Klaue (1998) Design of an epidemiologic study of drinking water arsenic exposure and skin and bladder cancer risk in a U.S. population. Environ. Health Perspect. 106: 1047–1050.
Neff, J. M. (1997) Ecotoxicology of arsenic in the marine environment. Environ. Toxicol. Chem.16: 917–927.
Ferguson, J. F. and J. Gavis (1972) A review of the arsenic cycle in natural water. Water Res. 6: 1259–1274.
Katsoyiannis, I., A. Zouboulis, H. Althoff, and H. Bartel (2002) As(III) removal from groundwaters using fixedbed upflow bioreactors. Chemosphere 47: 325–332.
Zhang, Q. L., N. Y. Gao, Y. C. Lin, B. Xu, and L. S. Le (2007) Removal of arsenic(V) from aqueous solutions using iron-oxide-coated modified activated carbon. Water Environ. Res. 79: 931–936.
Malik, A. (2004) Metal bioremediation through growing cells. Environ. Int. 30: 261–278.
Kuyucak, N. and B. Volesky (1988) Biosorbents for recovery of metals from industrial solutions. Biotechnol. Lett. 10: 137–142.
Gadd, G. M. (1990) Metaltolerance. pp. 178–210. In C. Edwards (eds.). Microbiology of extreme environments. McGraw-Hill, NY, USA.
Gadd, G. M. and C. White (1993) Microbial treatment of metal pollution-a working biotechnology? Trends Biotechnol. 11: 353–359.
Hamer, D. H. (1986) Metallothionein. Annu. Rev. Biochem. 55: 913–951.
Kapoor, A. and T. Viraraghavan (1995) Fungal biosorption- an alternative treatment option for heavy metal bearing wastewaters: a review. Bioresour. Technol. 53: 195–206.
Yanish-Perron, C., J. Vieira, and J. Messing (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene. 33: 103–119.
Zhao, Q., X. M. Liu, Y. Zhan, J. Q. Lin, W. M. Yan, J. Bian, and Y. Liu (2005) Construction of an engineered Acidithiobacillus caldus with high-efficiency arsenic resistance. Wei Sheng Wu Xue Bao. 45: 675–679.
Sambrook, J. and D. W. Russell (2001) Molecular Cloning: A Laboratory Manual. 3rd ed., pp. 1175–1182. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
Ru, B. G., A. H. Pan, B. Q. Huang, and J. Y. Zhang (1991) Metallothionein. Adv. Biochem. Biophys. 4: 254–259, 289.
Jiang, G., Z. Gong, X. F. Li, W. R. Cullen, and X. C. Le (2003) Interaction of trivalent arsenicals with metallothionein. Chem. Res. Toxicol. 16: 873–880.
Duncan, K. E. R., T. T. Ngu, J. Chan, M. T. Salgado, M. E. Merrifield, and M. J. Stillman (2006) Peptide folding, metal-binding mechanisms, and binding site structures in metallothioneins. Exp. Biol. Med. 231: 1488–1499.
Liu, J., Y. Liu, R. A. Goyer, W. Achanzar, and M. P. Waalkes (2000) Metallothionein-I/II null mice are more sensitive than wild-type mice to the hepatotoxic and nephrotoxic effects of chronic oral or injected inorganic arsenicals. Toxicol. Sci. 55: 460–467.
Chen, S. and D. B. Wilson (1997) Genetic engineering of bacteria and their potential for Hg2+ bioremediation. Biodegradation 8: 97–103.
Tian, K. L., J. Q. Lin, X. M. Liu, Y. Liu, C. K. Zhang, and W. M. Yan (2003) Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans. Biotechnol. Lett. 25: 749–754.
Lin, J. Q., J. B. Peng, and W. M. Yan (2001) Progress of the studies on gene transfer system of Thiobacillus Ferrooxidans. Chinese J. Appl. Environ. Biology 193-196.
Tripathi, R. D., S. Srivastava, S. Mishra, N. Singh, R. Tuli, D. K. Gupta, and F. J. M. Maathuis (2007) Arsenic hazards: strategies for tolerance and remediation by plants. Trends Biotechnol. 25: 158–165.
Valls, M., R. Gonzalez-Duarte, S. Atrian, and V. De Lorenzo (1998) Bioaccumulation of heavy metals with protein fusions of metallothionein to bacterial OMPs. Biochimie. 80: 855–861.
Kao, W. C., Y. P. Chiu, C. C. Chang, and J. S. Chang (2006) Localization Effect on the Metal Biosorption Capability of Recombinant Mammalian and Fish Metallothioneins in Escherichia coli. Biotechnol. Prog. 22: 1256–1264.
Bae, W., A. Mulchandani, and W. Chen (2002) Cell surface display of synthetic phytochelatins using ice nucleation protein for enhanced heavy metal bioaccumulation. J. Inorg. Biochem. 88: 223–227.
Kim, S. K., B. S. Lee, D. B. Wilson, and E. K. Kim (2005) Selective cadmium accumulation using recombinant Escherichia coli. J. Biosci. Bioeng. 99: 109–114.
Xiong, Y. and B. Ru (1997) Purification and characteristics of recombinant mouse metallothionein-I from Escherichia coli. J. Biochem. 121: 1102–1106.
Chen, S. and D. B. Wilson (1997) Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments. Appl. Environ. Microbiol. 63: 2442–2445.
Takeuchi, M., H. Kawahata, L. P. Gupta, N. Kita, Y. Morishita, Y. Ono, and T. Komai (2007) Arsenic resistance and removal by marine and non-marine bacteria. J. Biotechnol. 127: 434–442.
Teclu, D., G. Tivchev, M. Laing, and M. Wallis (2008) Bioremoval of arsenic species from contaminated waters by sulphate-reducing bacteria. Water Res. 42: 4885–4893.
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Su, YJ., Lin, JQ., Lin, JQ. et al. Bioaccumulation of Arsenic in recombinant Escherichia coli expressing human metallothionein. Biotechnol Bioproc E 14, 565–570 (2009). https://doi.org/10.1007/s12257-008-0197-y
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DOI: https://doi.org/10.1007/s12257-008-0197-y