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Effect of Curcumin-Hydroxypropyl-\(\beta\)-Cyclodextrin Complex and the Complex Loaded Gelatin Carrageenan Microparticles on the Various Chemical and Biological Properties

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Abstract

Curcumin was modified with 2-hydroxypropyl-\(\beta\)-cyclodextrin (HP\(\beta\)CD) to enhance its bioavailability. The modified curcumin was loaded into gelatin-carrageenan microparticles to control the drug release behavior. The different analytical techniques like Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy (RS), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM) indicated the formation of the samples. The solubility of the modified curcumin was checked visibly and by using UV-VIS spectroscopy & optical microscopy as well. The effect of surfactant on process yield, drug loading & encapsulation efficiency, swelling and drug release from the microparticles was checked. The samples exhibited more swelling and hence drug release was more in basic compared to acidic medium and the percentage increased with increase in time. The modified curcumin, on examining in both breast and lung cancer cell lines, manifested better anticancer activity compared to curcumin as evidenced by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, clonogenic assay and apoptosis assay. However, the microparticles didn’t reveal better anticancer activities compared to curcumin and modified curcumin. Further, all the prepared samples were found to be non-toxic to human peripheral blood mononuclear cells (PBMCs) and red blood cells (RBCs).

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Data Availability

The datasets generated during and/or analysed during the current study are not publicly available due to some pending research work using the data

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Acknowledgements

The authors would like to thank Tezpur University for the necessary technical facilities and infrastructure to carry out the research work. The authors would like to acknowledge Dr. Rajendra Joshi, Founder & CEO at RI Instruments & Innovation INDIA for the instrumental facility of Raman Spectroscopy. Financial support in the form of Maulana Azad National Fellowship, UGC, Govt. of India is highly acknowledged by the author Bably Khatun.

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No funding was received for conducting this study

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Authors and Affiliations

  1. Department of Chemical Sciences, Tezpur University, Napaam, Tezpur, 784028, India

    Bably Khatun & T. K. Maji

  2. Department of Molecular Biology & Biotechnology, Tezpur University, Napaam, Tezpur, 784028, India

    Munmi Majumder, R. Mukhopadhyay, Rafika Yasmin & Robin Doley

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  1. Bably Khatun
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Correspondence to T. K. Maji.

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The authors have the consent from Tezpur University Ethical Committee (TUEC) Memo No. DoRD/TUEC/10-14/3086-A for the use of isolated lymphocytes for the experiment. Blood sample was given voluntarily by me (Bably Khatun) with the help of Institutional health centre.

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Khatun, B., Majumder, M., Mukhopadhyay, R. et al. Effect of Curcumin-Hydroxypropyl-\(\beta\)-Cyclodextrin Complex and the Complex Loaded Gelatin Carrageenan Microparticles on the Various Chemical and Biological Properties. J Pharm Innov 17, 806–820 (2022). https://doi.org/10.1007/s12247-021-09559-0

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