Abstract
Endophytic fungi are rich sources of structurally complex chemical scaffolds with interesting biological activities. However, their metabolome is still unknown, making them appealing for novel compound discovery. To maximize the number of secondary metabolites produced from a single microbial source, we used the “OSMAC (one strain–many compounds) approach.” In potato dextrose medium, M. phaseolina produced phomeolic acid (1), ergosterol peroxide (2), and a volatile compound 1,4-benzene-diol. Incorporating an epigenetic modifier, sodium valproate, affected the metabolite profile of the fungus. It produced 3-acetyl-3-methyl dihydro-furan-2(3H)-one (3) and methyl-2-(methyl-thio)-butyrate (4), plus volatile chemicals: butylated hydroxy toluene (BHT), di-methyl-formamide, 3-amino-1-propanol, and 1,4-benzenediol, 2-amino-1-(O-methoxyphenyl) propane. The structure of compounds 1–4 was established with the help of spectroscopic data. This study revealed first-time compounds 1–4 in the fungus M. phaseolina using a classical and epigenetic manipulation approach.
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Acknowledgements
We thankfully acknowledge the Council of Scientific and Industrial Research (CSIR) for providing the platform for research. MK acknowledges the DBT, Govt. of India, for financial assistance (BT/PR4669/PBD/17/784/2012), while GS acknowledges the ICMR for the SRFship (ISRM/11(76)/2017). The manuscript bears IIIM Communication No. CSIR-IIIM/IPR/00360.
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Singh, G., Kumar, A., Verma, M.K. et al. Secondary metabolites produced by Macrophomina phaseolina, a fungal root endophyte of Brugmansia aurea, using classical and epigenetic manipulation approach. Folia Microbiol 67, 793–799 (2022). https://doi.org/10.1007/s12223-022-00976-3
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DOI: https://doi.org/10.1007/s12223-022-00976-3