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Electrospun Porcine Acellular Dermal Matrix and Polycaprolactone Composite Nanofibrous Scaffolds for Accelerating Wound Healing

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Abstract

Electrospun nanofibers represent a novel class of scaffold materials that show great potential in wound healing owing to relatively large surface area, better mimicry of native extracellular matrix, adjustable waterproofness and breathability, and programmable drug delivery process. In this work, electrospun polycaprolactone (PCL) and porcine acellular dermal matrix (PADM) composite nanofibrous membranes have been developed by electrospinning technology and inflated into three-dimensional (3D) structural scaffold (PCL-PADM) by gas foaming method. The obtained PCL-PADM has higher thermal stability, hydrophilicity and better cell adhesion compared with PCL. In addition, ε-polylysine (ε-PL) is further attached onto the surface of PADM/PCL to offer its good antibacterial properties. Moreover, the PADM/PCL fibrous scaffolds show excellent cytocompatibility for promoting cell proliferation. In vivo models showed that the resultant PADM/PCL fibrous scaffolds exhibit an accelerating wound healing effect through promoting expression of vascular factor (CD31) and decreasing the expression of tumor necrosis factor-α (TNF-α). These results indicate that the 3D fibrous scaffolds may be a potential wound dressing for wound closure.

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Data Availability

The authors declare that all data supporting the findings of this study are available within the paper and its supplementary information; source data for the figures in this study are available from the online version.

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Acknowledgements

This work was supported by the Natural Science Foundation of Zhejiang Province (LBY20H110001, LY18E030006) and National Natural Science Foundation of China (51873194).

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Authors and Affiliations

  1. School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Xiaofei Gao, Guohua Jiang & Yanting Jing

  2. International Scientific and Technological Cooperation Base of Intelligent Biomaterials and Functional Fibers, Zhejiang Sci-Tech University, Hangzhou, 310018, China

    Guohua Jiang

  3. Department of Dermatology, Beilun People’s Hospital of Ningbo City, Ningbo, 315800, China

    Liming Ruan

  4. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China

    Yanfang Sun

  5. Institute of Polymer Chemistry and Physics, Uzbekistan Academy of Sciences, 100128, Tashkent, Uzbekistan

    Khaydar E. Yunusov

  6. Research Institute for Physical Chemical Problems of the Belarusian State University, 220030, Minsk, Belarus

    Uladzislau E. Aharodnikau & Sergey O. Solomevich

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  1. Xiaofei Gao
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  2. Guohua Jiang
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  3. Liming Ruan
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Contributions

GJ: conceptualization, funding acquisition, methodology, supervision, data curation, writing—review and editing, YS: conceptualization, methodology, supervision, XG: data curation, methodology, visualization, writing—original draft, KEY: methodology, investigation, RW: data curation, validation, PL: formal analysis, writing—review and editing, UEA: formal analysis, writing—review and editing, SOS: writing—review and editing, validation.

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Correspondence to Guohua Jiang or Liming Ruan.

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Gao, X., Jiang, G., Ruan, L. et al. Electrospun Porcine Acellular Dermal Matrix and Polycaprolactone Composite Nanofibrous Scaffolds for Accelerating Wound Healing. Fibers Polym 24, 589–601 (2023). https://doi.org/10.1007/s12221-023-00077-z

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