Strategy for Synthesizing Novel Acetamidines as CO2-Triggered Switchable Surfactants via Acetimidates

In this study, we developed a strategy for using the Scoggins procedure in the synthesis of acetamidines as novel CO2-triggered switchable surfactants via acetimidates by effectively tuning the chemical equilibrium. The as-synthesized N’-alkyl-N,N-diethylacetamidines exhibit excellent CO2/N2 switchability and their bicarbonate salts have the ability to emulsify oil–water mixtures.


Introduction
Surfactants, whose amphiphilic structures enable the stabilization of emulsions with two immiscible phases, are widely applied in many processes such as cleaning [1], viscous oil transportation [2], and enhancing oil recovery [3]. Excellent stabilization is required in these processes, usually followed by a subsequent emulsion-destabilization step to effectively recover the two immiscible phases. However, this remains a challenging task for most commonly used surfactants. To tackle this problem, it is necessary for the surfactants that can be "switched on and off" to reversibly stabilize/destabilize the emulsion under certain trigger conditions. The switchable surfactants reported in the literature can undergo reversible interconversions from surface-active to surfaceinactive forms upon command, and their switchability can usually be triggered by altering the external conditions, such as pH [4], electrochemical redox [5], magnetic-field intensity [6], or light source (UV or visible light) [7]. Compared with these triggers, CO 2 is environmentally benign, inexpensive, and renewable, and more important, it does not accumulate in a system after repeated cycles. Therefore, it has been identified as an innovative and ideal "trigger" for stimuli-responsive surfactants and has recently been studied for use in reversible solvents [8][9][10], switchable Pickering emulsions [11,12], and repeatable polymer-based materials [13,14], particularly for application in enhanced oil recovery [15,16].
Thus far, the majority of CO 2 /N 2 reversible surfactants include guanidines, imidazoles, tertiary amines, and amidines. In their inactive forms, these surfactants can be protonated to form charged bicarbonates that become active upon the addition of CO 2 in aqueous media, increasing their solubility in water. The bicarbonate ions can then be deprotonated by bubbling with N 2 or air [17]. However, these common CO 2 /N 2 reversible surfactants have some inherent drawbacks that hinder their wide application. The headgroup of imidazole surfactants limits their triggering efficiency due to their hydrolysis in aqueous media [18]. Surfactants containing a hydrophilic headgroup of guanidine are generally basic and require more rigorous conditions to achieve deprotonation (such as high temperature, long reaction time, or heavy flow rate of bubbling gas) [19].

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With respect to tertiary amines, their basicity is so weak that stable emulsions can hardly be formed [20]. As such, compounds containing an amidine head [21,22] are attracting more attention due to their mild basicity and superior stability. In addition, the exceptional demulsifying ability of amidines in their neutral form is an unexpected advantage. Scheme 1 illustrates the protonation and deprotonation processes of amidines stimulated by CO 2 and N 2 (or air), respectively, in aqueous media, where R represents a longchain alkyl or ethoxylated groups. Amidine surfactants can easily and reversibly transform into bicarbonate salts.
Amidines can be synthesized using various strategies, such as the nucleophilic addition of amines to nitriles [23], reaction of catalytic three-component coupling [24], displacement reaction of imidates with amines [25,26], and the Scoggins procedure [27,28]. Of these synthetic methods, the Scoggins procedure involving primary amines and N,N-dimethylacetamide dimethyl acetal can synthesize long-chain aliphatic amidines and aromatic amidines with a high yield of 90% accompanied by the formation of a small amount of imidates as a by-product [27,28]. This is particularly attractive since an encouraging acetamidines yield of 99% was achieved by Jessop et al. [21] when they added an excess amount of dimethylamine to the reaction system. However, the types of amidines used in this synthesis method have been fairly limited thus far, due to the limitation of commercially available reactants. The structure of amidines generated by this method differed from those in the hydrophobic tail group, which are mainly long-chain alkyl or ethoxylated groups, or both [16,21], and the hydrophilic head is bonded only with the dimethyl group.
As stated earlier, imidates were the only by-product of the Scoggins procedure. At the same time, the potent electrophilicity of imidates supports the synthesis of amidines through displacement reactions with amines. Therefore, imidates have potential for use in the synthesis of new CO 2 /N 2 switchable amidines if various imidates can be generated as the main product by modifying the Scoggins procedure. We also believe it is important to gain insight into the reaction path of the Scoggins procedure to facilitate the development of this novel synthesis strategy. In this study, we mainly investigated the reaction path of the Scoggins procedure for use in developing a new approach for the synthesis of novel CO 2 /N 2 switchable surfactants.

General Procedure for the Synthesis of N-alkyl-O-Methylacetimidates
We put N,N-dimethylacetamide dimethyl acetal (1.72 g, 0.012 mol) and dodecyl amine (1.89 g, 0.01 mol) into a three-neck round-bottom brown flask in a dark environment and an oil bath, to which we then added methanol (40 mL). We kept this mixture at 68 °C for 2 h with a methanol reflux in the condenser. To determine when the conversion of the dodecylamine was complete, we used the thin-layer chromatography method (Merck TLC plates, silica gel 60 F254, with chloroform and methanol as developing solvents, V CHCl3 /V CH3OH = 3:1). Then, we removed the methanol and dimethylamine from the reaction mixture by rotary evaporation at 50 °C and − 0.1 MPa for 20 min, obtaining a light-yellow liquid product consisting of N-dodecyl-O-methylacetimidate (1a, 71.7% yield) and N'-dodecyl-N,Ndimethylacetamidine (1b, 28.3% yield). We calculated the yield based on its 1 H NMR spectrum. Then, we subjected the resulting mixture to flash column chromatography on aluminum oxide (active, neutral, 200-300 mesh) using ethyl acetate and dichloromethane (V CH3COOCH2CH3 /V CH2Cl2 = 1:2) as mobile phases to obtain purified N-dodecyl-O-methylacetimidate (1a) as a colorless liquid. We also synthesized Scheme 1 Protonation and deprotonation processes of amidines and bicarbonate salts  Fig. 1 We added N,N-dimethylacetamide dimethyl acetal (1.72 g) and dodecyl amine (1.89 g) to a three-neck round-bottom brown flask in a dark environment, to which we added 40 mL of methanol. Then we maintained the mixture at 68 °C for 2 h by controlling the temperature of the oil bath with a methanol reflux in the condenser. We extracted 1 mL samples at intervals and immediately transferred them into an ice bath. Then, we removed the methanol and dimethylamine from the samples using a rotary evaporator at 10 °C and − 0.1 MPa. The remaining product was a mixture of N-dodecyl-O-methylacetimidate (1a) and N'dodecyl-N,N-dimethylacetamidine (1b). Based on their 1 H NMR spectra, we calculated the selectivities of 1a and 1b at different reaction times.  Experimental Process for the Results Shown in Fig. 2 First, we prepared a certain amount of purified N'-dodecyl-N,N-dimethylacetamidine (1b) using the method developed by Jessop et al. [21]. We added about 0.012 mol of N,N-dimethylacetamide dimethyl acetal (1.72 g) and 76.4 mmol of Me 2 NH (38 mL, 2 mol/L solution in tetrahydrofuran) into a three-neck round-bottom brown flask and stirred the mixture (100 r/min) at 25 °C. After that, we added about 0.01 mol dodecylamine (1.89 g) dropwise to this mixture at room temperature, stirred it for 10 min, and then left it for 18 h in the dark. To remove most of the tetrahydrofuran and Me 2 NH, we kept the mixture at 55 °C and − 0.1 MPa in a rotary evaporator for 8 h, which yielded a yellow liquid consisting of N'dodecyl-N,N-dimethylacetamidine (1b, yield = 97.6%) and a trace impurity of N-dodecyl-O-methylacetimidate (1a, yield = 2.4%), which we identified based on its 1 H NMR spectrum. We then reacted the above product (2.54 g) with methanol (40 mL) in a three-neck round-bottom brown flask with a condenser and thermometer and kept this mixture at 68 °C for 2 h. We extracted 1 mL samples at intervals, which we immediately transferred into an ice bath. Then, we removed the methanol and dimethylamine from the samples using a rotary evaporator at 10 °C and −0.1 MPa. By 1 H NMR, we identified the remaining product as a mixture of N-dodecyl-O-methylacetimidate (1a) and N'-dodecyl-N,N-dimethylacetamidine (1b). Based on their 1 H NMR spectra, we calculated the normalized concentrations of 1a and 1b at different reaction times.

General Procedure for the Switchable Characterization Tests on N'-Octadecyl-N,N-Diethylacetamidine
We determined the conductivity of the solution bubbled by CO 2 or N 2 using a DDSJ-308F conductivity meter (Shanghai Leici, China) and controlled the gas-flow rate using a mass flow controller (Beijing Shengye, China). We bubbled the solution of DMSO and water (V DMSO /V water = 45 mL/5 mL) with N'-octadecyl-N,N-diethylacetamidine (4c, 0.22 g) by CO 2 , followed by N 2 at 65 °C, during which we recorded the conductivity. We obtained four reversible cycles by bubbling CO 2 (400 mL/min) for 40 min and then N 2 (800 mL/min) for 40 min. We also determined the conductivity of the solution containing purified N-octadecyl-O-methylacetimidate (4a, 0.08 g) using the same method as that in the blank experiment. We examined the capability of stabilizing an emulsion by mixing dodecane and water (5 mL, v/v = 1:1) containing N'-octadecyl-N,N-diethylacetamidine (4c, 0.022 g) and observed the state of the oil-water emulsion layers formed by the bubbling CO 2 at different stages.

Consecutive Reactions of Scoggins Procedure in the Presence of Excess Methanol
To effectively regulate product distribution in the Scoggins procedure, we conducted this reaction by varying the organic additives. Table 1 summarizes the reaction results based on the 1 H NMR characterization. Compared with the results (Fig. S2) obtained in experiment 1 using the Oszczapowicz' method [28] without any additives, we obtained a high N'-dodecyl-N,N-dimethylacetamidine (1b) yield of about 97.6% (Fig. S3) when using NH(CH 3 ) 2 as the additive, as developed by the Jessop et al. [21]. It seems that NH(CH 3

) 2 can inhibit the formation of N-dodecyl-O-methylacetimidate.
Interestingly, the addition of methanol (experiment 3) led to a dramatic decrease in the yield of 1b, which represents only 28.3% of the total conversion of dodecylamine, whereas we found 1a to be the only by-product with a yield of 71.7% (Fig. S4). This remarkable decline in the acetamidine yield and the formation of such a large amount of acetimidate demonstrates that the presence of methanol may control the product distribution to a great extent.
To further understand the reaction path of the Scoggins procedure in the presence of excess methanol, we designed an experiment (experiment 3), in which we performed interval sampling during the reaction process to obtain a detailed product distribution as a function of the reaction time. We used 1 H NMR to analyze the relative selectivity of acetamidine and acetimidate during the reaction. Figure 1 shows plots of the evolution of the selectivities of 1a and 1b versus reaction time. In Fig. 1, we can see that the selectivity of 1b increases rapidly to its peak within 2 min and then decreases slowly until reaching a constant selectivity of 28.1%, whereas the selectivity of 1a increases gradually until reaching 71.9% at 120 min. These results indicate that the process is likely a consecutive reaction, in which 1b is easily formed as an intermediate in the first step and then reacts with methanol to generate 1a in the second step. The fact that 1b is readily generated in this reaction may be due to a bimolecular nucleophilic substitution reaction (SN2) between N,N-dimethylacetamide dimethyl acetal with high electrophilicity and the nucleophilic reagent dodecylamine. This reaction may be constrained by a thermodynamic equilibrium, in which the excess methanol in the mixture promotes the generation of acetimidates.
To better confirm the consecutive reaction pathway of this reaction system, we performed a reaction between 1b (synthesized during experiment 2 in advance) and methanol. Using 1 H NMR analysis, we identified only 1a and 1b in the resulting mixture by after removing the volatile phase from the samples in high vacuum conditions. Figure 2 shows the normalized concentrations of 1a and 1b in the samples as a function of reaction time. We can see that the normalized concentration of 1b significantly decreases with reaction time, reaching a constant value of about 28.3% after about 90 min. The normalized concentration of 1a shows the opposite trend at first and ultimately remains constant at 71.7%. The time required to reach the equilibrium concentration is about 90 min, which is shorter than that of the Scoggins procedure (120 min) in the conditions described above. These results indicate that 1a is generated from the reaction between 1b and methanol, thereby providing further evidence that the Scoggins procedure involves a consecutive reaction. In addition, the higher selectivity of 1b at the beginning stage of the Scoggins procedure and the lower equilibrium yield of 1b due to the limiting effect of the thermodynamic equilibrium prove that the reaction of 1b with methanol in the second step is the control step for the formation of 1a.
Based on the above findings, we successfully synthesized a class of N-alkyl-O-methylacetimidates (A, Fig.  S1) by enhancing the Scoggins procedure with the excess methanol (using the same reaction conditions as experiment 3 in Table 1). As listed in Table 2, all the yields of A are around 70% (Figs. S4-S7), which means that all these reactions reach roughly the same level of equilibrium regardless of the different hydrophobic groups of the primary amines. Next, we purified a series of product A to colorless liquids using flash column chromatography with Al 2 O 3 as the stationary phase and ethyl acetate/hexane (v/v, 2:3) as the eluent. We then confirmed the resulting structures by HRMS (Figs. S8-S11), 1 H NMR (Figs. S14, S17, S20, S23), 13 C NMR (Figs. S15, S18, S21, S24), and IR spectroscopy (Figs. S32-S35). Taking into consideration the characteristic of the equilibrium reaction, these acetimidates might have potential for use as precursors in the preparation of novel acetamidines via reactions with different primary or secondary amines.

Synthesis of Novel Acetamidines via Acetimidates
To verify the feasibility of generating new acetamidines from acetimidates, we performed a series of reactions between purified A and NH(CH 2 CH 3 ) 2 . As expected, we successfully produced two N'-alkyl-N,N-diethylacetamidines (C) with a diethyl group linked to the amidine group for the first time via a displacement reaction (Table 3) with an The conversion of primary amines is 100% a The acetimidates yield was calculated based on the relative integration of the peak at δ H = 3.60 with respect to δ H = 2.87, which represents the groups of -O(CH 3 ) and -N(CH 3 ) 2 , respectively b The acetamidines yield was calculated based on the relative integration of the peaks at δ H = 2.87 and δ H = 3.60 c We observed no characteristic peak at δ H = 2.87 The selectivity to C is 100%, from which C is obtained as the exclusive product a The conversion of acetimidates b The yields of 3c and 4c were calculated from the relative integration of the quartet near δ H = 3.30 (4H, -N(CH 2 CH 3 ) 2 ) [30] with the triplet at δ H = 3.60 (3H, -OCH 3 ) based on the 1 H NMR spectrum extremely high selectivity of nearly 100%. We confirmed the structures of N'-hexadecyl-N,N-diethylacetamidine (3c) and N'-octadecyl-N,N-diethylacetamidine (4c) by HRMS (Figs. S12, S13), 1 H NMR (Figs. S26, S29), 13 C NMR (Figs. S27, S30) and IR spectroscopy (Figs. S36, S37). The quartets at δ H = 3.29 in the 1 H NMR spectra and at δ C = 41.68 in the 13 C NMR spectra indicate methylene in the -N(CH 2 CH 3 ) 2 group. We also detected solution peaks of trace 1,4-dioxane at δ H = 3.70 and δ C = 67.03 [30]. This strategy opens a new possible way to design and efficiently synthesize novel acetamidines with various terminal alkyl groups by simply replacing NH(CH 2 CH 3 ) 2 with other dialkylamines.

CO 2 -Triggered Switchable Characterization of Novel Acetamidines
Next, we examined the CO 2 /N 2 switchability of N'-octadecyl-N,N-diethylacetamidine by monitoring the conductivity in wet dimethyl sulfoxide. As shown in Fig. 3, N'-octadecyl-N,N-diethylacetamidine (4c) exhibited excellent switchability when CO 2 and then N 2 were bubbled through the solution over four cycles, which indicates the reversible transformation between neutral amidine and bicarbonate ions. We also examined the capability of N'-octadecyl-N,N-diethylacetamidine bicarbonates salts to stabilize an emulsion by mixing dodecane and water containing 4c. As shown in Fig. 4, the two immiscible phases became a homogeneous emulsion after the bubbling of CO 2 . When kept at room temperature for 12 h, the emulsion volume still accounted for about 90% of the oil-water system, including a thin cloudy liquid at the bottom of the measurement cylinder (Fig. 4c). Then, bubbling N 2 rapidly breaks the emulsion into two separate phases (Fig. 4d). The conductivity and emulsification tests containing only 4a evidenced that the rest of 4a had little effect and can be ignored. These results demonstrate that the synthesized N'-alkyl-N,N-diethylacetamidines have CO 2 /N 2 switchability in the emulsification and demulsification of the oil-water system.

Conclusions
In conclusion, in this study, we developed a new approach for synthesizing CO 2 -triggered switchable surfactants by the reaction between amines and imidates, produced using an enhanced Scoggins procedure. This reaction process consists of two reactions in a series, and the yield of acetimidates can be significantly improved by shifting the reaction equilibrium using excess methanol as an additive. This method provides an effective route for synthesizing varieties of CO 2 /N 2 switchable acetamidines with the desired functional groups bonded to the amidine group.
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