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An Antibody Ordered Assembly Functional BPE-ECL Platform for Aflatoxin B1 Detection

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Abstract

Due to the complex characteristics of food matrix, false positives may occur when using traditional methods to detect aflatoxin B1 (AFB1). In this paper, we utilize the advantages of closed bipolar electrode (BPE) that can effectively avoid the impact of complex food matrices on signal collection, and design a biosensor based on immune competition to detect highly toxic AFB1. The biosensor uses cathode of closed BPE as a functional sensing interface and anode as a signal collection interface. On the functional sensing interface, AFB1 connects to the monoclonal antibody at the top of DNA tetrahedron structure. Under the action of horseradish peroxidase (HRP), 4-chloro-1-naphthol (4-CN) is catalyzed by hydrogen peroxide (H2O2) to generate precipitations (4-CD). AFB1 competes with HRP-AFB1 to bind monoclonal antibody, resulting in a decrease in the content of HRP involved in catalytic oxidation reactions and a synchronous reduction in precipitations produced, causing an increase in the intensity of [Ru(bpy)3]2+/TPA system electrochemiluminescence (ECL) on the BPE anode. Analysis shows that the relative deviation between the detection results and ELISA is between −4.5 and 9.8%, and there is no significant difference in the performance of the two in detecting AFB1. The BPE-ECL sensor exhibits high sensitivity and specificity in detecting AFB1, with a linear range of 0.01–40 ng mL−1 and a detection limit of 3 pg mL−1. In the future, it can be applied to the detection of other toxins, with broad application prospects.

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Data will be made available on request.

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Funding

This work was financially supported by the National Natural Science Foundation of China (No. 21804071) and the Natural Science Foundation of Jiangsu Province of China (No. BK20180688).

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Authors and Affiliations

  1. Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing, 211800, China

    Fanglin Qiao, Yin Chen, Yunjie Fei, Yanjun Jiang, Xiaohui Xiong & Yuanjian Liu

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  1. Fanglin Qiao
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  2. Yin Chen
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  3. Yunjie Fei
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  4. Yanjun Jiang
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  5. Xiaohui Xiong
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Correspondence to Yuanjian Liu.

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Conflicts of Interests

Fanglin Qiao declares no competing interests. Yin Chen declares no competing interests. Yunjie Fei declares no competing interests. Yanjun Jiang declares no competing interests. Xiaohui Xiong declares no competing interests. Yuanjian Liu declares no competing interests.

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Qiao, F., Chen, Y., Fei, Y. et al. An Antibody Ordered Assembly Functional BPE-ECL Platform for Aflatoxin B1 Detection. Food Anal. Methods 17, 260–268 (2024). https://doi.org/10.1007/s12161-023-02558-6

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